Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
5541.1.64_GO.34680
Keyword:
full-length cds (mutant)
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
ScCD00534175
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2838nts
Open reading frame: 51 to 2888

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCAGCAATGATCCATCTGCTGTATCGGAACTACCAGACAAGGACAGTCTTG
ATAACGGTATCAGCAATGACAATGAAAGGGCCATGGGCGGCAATGGCGATGGCGGCGATG
GATTACGATTACCAAGGACCACTGGAACTTTGAACGTCAATGCCTTACAAAAAGGCACTA
ATGCTGCCCATGAAGCTGGTGGATACAAATCCATGGATCCTGCGAAGAACGCGGAGACAA
CCAATGATGATGACAATAATGTCGTTTCACTAGATGACCCTATTCAATTTACCCGGGTAT
CTTCCTCCTCTGTCATCAGTGGAATGTCTTCATCCGTGAGTCCTCATTCTAACATCGATG
AAACCAAATCTCTAGAAGCAGTCACTCCAAACATAAATACCAGCAATATGACCCCGGATC
ATTCTGCTGACAACACATTTTCTACCATAAATGCGTCCGAGTCAGATCACCAGTTTAATG
ACACTTTACTATCAAAACTGTCGTTAACAGATTCTACAGAAACTATAGAAAATAACGCGA
CAGTGAAGCACCAGCAGCCAGTTGCATCTTCCACAGTAAACTCGAATAAGAGCTCCACTG
ATATACGAAGGGCTACACCAGTGTCCACTCCCGTTATCTCTAAACCATCGATGACAACCA
CGCCAAGACAGATCAATTCAGCTTCCCATTCGCTTTCGAACCCTAAGCATAAGCAACATA
AACCAAAAGTTAAACCGTCCAAGCCTGAAGCAAAAAGTAAACCGGTTTCTGTGAAAAAAA
GCTTTCCTTCGAAAAATCCTTTAAAAAACTCCTCTCCATCTAAAAAGCAAACAGAAAAAT
CGTATTATTCTTCCTCTTCGAAAAAAAGGAAAAGCGGTTCAAATAGTGGTACACTAAGAA
TGAAAGATGTCTTTACGTCCTTTGTACAGAATATAAAGAGAAATTCTCAGGATGATAAAA
GGGCCTCATCGTCGTCCAATAATTCTTCCTCATCTTCTATAACCACCGCTTTGAGGATAT
CTACGCCATACAATGCCAAGCATATCCACCATGTGGGCGTGGACTCCAAGACTGGTGAGT
ACACAGGTTTGCCGGAGGAATGGGAAAAATTGTTGACTTCTAGTGGTATTTCCAAAAGAG
AACAACAGCAAAACATGCAAGCAGTCATGGATATTGTCAAATTCTATCAGGATGTCACGG
AAACAAACGGTGAAGATAAAATGTTCAAGACTTTCAACACAACCACAGGATTGCCGGGAA
ATCCTCAAGTTTCAACACCGCCTGCAAACTCATTCAATAAATTTCCTCCGTCGACAAGTG
ATTCGCACAATTACGGTTCCAGAACAGGTACACCAATGTCCAATCACGTCATGTCTCCAA
CCTTAAATACAGATTCTAGTTCAGCAAACGGGAAATTCATACCAAGTAGACCGGCTCCTA
AGCCCCCATCTTCTGCGTCCGCTTCAGCTCCAATTATAAAATCACCCGTCATGAATTCTG
CCGCCAATGTTTCGCCCTTGAAGCAGACTCATGCACCTACAACTCCGAACAGAACCAGCC
CAAACAGGTCCTCAATATCAAGAAATGCCACTTTAAAAAAAGAGGAGCAGCCACTACCAC
CAATACCTCCAACCAAATCCAAAACATCTCCAATCATCTCCACAGCTCACACACCACAGC
AAGTTGCTCAATCGCCAAAAGCGCCGGCGCAAGAGACGGTAACGACACCTACTTCGAAGC
CAGCTCAAGCAAGAAGCTTGTCTAAAGAATTAAATGAGAAAAAGAGAGAGGAAAGGGAAA
GACGTAAAAAACAACTATATGCCAAATTGAACGAAATTTGCTCAGACGGTGACCCAAGTA
CAAAATATGCCAATTTAGTAAAAATTGGTCAAGGTGCATCAGGTGGTGTTTATACTGCTT
ATGAAATAGGTACGAATGTCTCAGTGGCCATTAAGCAAATGAATCTCGAAAAGCAACCAA
AAAAGGAGCTAATCATCAATGAGATTCTGGTCATGAAGGGTAGCAAACACCCTAATATAG
TTAATTTCATTGATTCTTACGTTTTAAAAGGCGACCTTTGGGTCATTATGGAATACATGG
AAGGTGGCTCCTTAACTGATGTGGTCACCCATTGTATTTTGACAGAAGGTCAAATTGGTG
CCGTTTGTAGAGAAACTTTGAGTGGGTTGGAATTTTTACATTCTAAAGGTGTTCTTCACA
GAGATATCAAATCCGATAACATCCTATTGTCCATGGAAGGGGATATTAAGTTAACGGATT
TCGGTTTTTGCGCTCAAATCAATGAATTGAACTTGAAAAGAACTACTATGGTGGGAACGC
CTTATTGGATGGCGCCTGAAGTGGTTTCTAGGAAAGAATATGGCCCAAAAGTAGATATCT
GGTCGTTGGGTATCATGATCATTGAAATGATCGAGGGGGAGCCTCCATATTTAAATGAAA
CCCCGCTAAGAGCACTGTATTTAATTGCTACAAATGGTACACCCAAGTTAAAGGAACCCG
AGAATCTATCGTCAAGCTTGAAAAAATTCCTTGATTGGTGTTTATGTGTGGAGCCCGAAG
ATAGAGCAAGCGCTACGGAATTGCTTCATGATGAATATATCACGGAGATAGCTGAAGCCA
ATTCCTCATTGGCCCCGCTAGTCAAGTTAGCAAGATTGAAGAAAGTAGCTGAGAACATGG
ATGCTGATGAAGATAATGACGACGATAACGACAACGAGCATATTAATAAGACAAACAATT
GTGACGACAATAACGATAGCAAAGAAACCGTAAATTTGGACGTAACTGAAGATGATAAAC
AAAAGTAATAGGACCCAGCTTTCTTGTACAAAGTTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSSNDPSAVSELPDKDSLDNGISNDNERAMGGNGDGGDGLRLPRTTGTLNVNALQ
KGTNAAHEAGGYKSMDPAKNAETTNDDDNNVVSLDDPIQFTRVSSSSVISGMSSSVSPHS
NIDETKSLEAVTPNINTSNMTPDHSADNTFSTINASESDHQFNDTLLSKLSLTDSTETIE
NNATVKHQQPVASSTVNSNKSSTDIRRATPVSTPVISKPSMTTTPRQINSASHSLSNPKH
KQHKPKVKPSKPEAKSKPVSVKKSFPSKNPLKNSSPSKKQTEKSYYSSSSKKRKSGSNSG
TLRMKDVFTSFVQNIKRNSQDDKRASSSSNNSSSSSITTALRISTPYNAKHIHHVGVDSK
TGEYTGLPEEWEKLLTSSGISKREQQQNMQAVMDIVKFYQDVTETNGEDKMFKTFNTTTG
LPGNPQVSTPPANSFNKFPPSTSDSHNYGSRTGTPMSNHVMSPTLNTDSSSANGKFIPSR
PAPKPPSSASASAPIIKSPVMNSAANVSPLKQTHAPTTPNRTSPNRSSISRNATLKKEEQ
PLPPIPPTKSKTSPIISTAHTPQQVAQSPKAPAQETVTTPTSKPAQARSLSKELNEKKRE
ERERRKKQLYAKLNEICSDGDPSTKYANLVKIGQGASGGVYTAYEIGTNVSVAIKQMNLE
KQPKKELIINEILVMKGSKHPNIVNFIDSYVLKGDLWVIMEYMEGGSLTDVVTHCILTEG
QIGAVCRETLSGLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINELNLKRTTM
VGTPYWMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKL
KEPENLSSSLKKFLDWCLCVEPEDRASATELLHDEYITEIAEANSSLAPLVKLARLKKVA
ENMDADEDNDDDNDNEHINKTNNCDDNNDSKETVNLDVTEDDKQK*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.34680
Recommended expression in:
Not Applicable