Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
5541.1.65_GO.34677
Keyword:
full-length cds
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
ScCD00534176
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2085nts
Open reading frame: 51 to 2135

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCAAAGTCCAAATAACCAATAGTAGAACAGAGGAAATCTTAAAAGTTCAAG
CTAATAATGAAAACGATGAAGTCAGTAAAGCTACTCCGGGCGAAGTTGAAGAATCGCTAA
GGTTAATCGGCGATCTAAAATTCTTTTTAGCCACAGCGCCGGTAAATTGGCAAGAAAACC
AAATTATAAGGCGATACTATCTGAATAGTGGACAAGGCTTTGTCTCTTGTGTATTTTGGA
ACAATCTATACTATATTACAGGTACTGATATTGTCAAATGTTGTCTTTACAGAATGCAAA
AGTTTGGGAGAGAAGTAGTTCAAAAGAAAAAATTTGAAGAGGGTATTTTTTCAGATTTAA
GAAATCTCAAATGTGGTATAGATGCAACTTTAGAACAACCAAAGTCCGAATTTTTGTCGT
TTCTATTCAGAAATATGTGTCTGAAAACCCAAAAAAAGCAGAAAGTATTTTTTTGGTTCA
GTGTAGCACATGATAAGTTGTTTGCGGATGCGTTGGAAAGAGATTTAAAAAGAGAAAGTT
TGAATCAGCCTTCAACGACTAAGCCCGTTAATGAGCCCGCCTTATCTTTTTCATATGATT
CCTCATCTGATAAGCCTCTCTACGATCAGTTACTTCAACATTTAGATTCTAGAAGACCAT
CTAGTACAACAAAATCAGATAATTCGCCTCCAAAATTAGAAAGCGAGAATTTTAAGGATA
ATGAGTTGGTAACAGTAACTAATCAGCCGCTTTTAGGCGTTGGCCTCATGGATGACGATG
CGCCAGAATCCCCCTCTCAAATTAATGATTTTATTCCTCAGAAATTGATTATAGAACCCA
ATACTCTCGAATTGAATGGTCTCACAGAAGAAACGCCTCATGACTTACCCAAGAATACCG
CTAAGGGCAGAGACGAAGAAGATTTTCCTCTCGACTATTTTCCTGTATCTGTTGAATACC
CTACGGAGGAAAATGCGTTTGATCCGTTCCCTCCACAGGCTTTTACGCCAGCTGCCCCTT
CCATGCCTATTTCCTATGATAACGTGAATGAAAGGGATTCTATGCCCGTTAATTCTCTTC
TTAATAGATACCCCTATCAGTTATCAGTGGCACCCACTTTCCCAGTGCCACCATCATCAT
CGAGGCAACATTTTATGACAAATCGGGATTTTTATTCATCTAACAATAACAAGGAAAAAT
TGGTATCTCCTAGCGACCCTACCAGCTACATGAAGTATGACGAACCAGTTATGGATTTTG
ATGAATCTCGGCCAAATGAAAACTGTACAAATGCAAAATCTCACAACTCTGGCCAGCAAA
CTAAACAACACCAATTATATTCTAACAACTTCCAGCAATCTTACCCAAACGGAATGGTTC
CAGGATACTACCCAAAAATGCCGTATAATCCCATGGGGGGGGATCCTCTACTCGATCAAG
CCTTTTATGGCGCGGACGATTTTTTCTTTCCACCAGAAGGATGTGATAACAATATGCTGT
ATCCACAAACTGCAACTTCATGGAATGTTTTGCCCCCTCAAGCTATGCAACCAGCTCCAA
CCTATGTTGGGAGACCATACACACCGAATTATAGATCGACACCAGGTTCCGCGATGTTCC
CATACATGCAAAGTTCAAATTCCATGCAGTGGAACACTGCTGTTTCACCTTATAGTTCGA
GAGCACCATCTACAACTGCTAAAAACTATCCTCCTAGCACATTTTATTCTCAAAATATAA
ATCAATACCCACGGCGAAGAACTGTGGGAATGAAGTCTTCACAAGGAAATGTTCCAACAG
GTAATAAACAATCTGTGGGCAAGTCTGCAAAAATTTCAAAGCCTCTACATATTAAGACAA
GTGCTTATCAGAAGCAATACAAAATCAACTTGGAAACGAAAGCCAGGCCAAGTGCTGGTG
ACGAAGATTCTGCTCATCCTGATAAGAACAAAGAAATTTCGATGCCTACTCCGGATTCCA
ATACTTTGGTGGTCCAGTCAGAAGAAGGTGGAGCTCATTCACTTGAGGTAGATACCAATC
GAAGGTCCGATAAAAACCTTCCAGATGCAACCTGATAGGACCCAGCTTTCTTGTACAAAG
TTGGCATTATAAGAAAGCATTGCTTA

Protein sequence predicted based on nucleotide sequence

ENLYFQSKVQITNSRTEEILKVQANNENDEVSKATPGEVEESLRLIGDLKFFLATAPVNW
QENQIIRRYYLNSGQGFVSCVFWNNLYYITGTDIVKCCLYRMQKFGREVVQKKKFEEGIF
SDLRNLKCGIDATLEQPKSEFLSFLFRNMCLKTQKKQKVFFWFSVAHDKLFADALERDLK
RESLNQPSTTKPVNEPALSFSYDSSSDKPLYDQLLQHLDSRRPSSTTKSDNSPPKLESEN
FKDNELVTVTNQPLLGVGLMDDDAPESPSQINDFIPQKLIIEPNTLELNGLTEETPHDLP
KNTAKGRDEEDFPLDYFPVSVEYPTEENAFDPFPPQAFTPAAPSMPISYDNVNERDSMPV
NSLLNRYPYQLSVAPTFPVPPSSSRQHFMTNRDFYSSNNNKEKLVSPSDPTSYMKYDEPV
MDFDESRPNENCTNAKSHNSGQQTKQHQLYSNNFQQSYPNGMVPGYYPKMPYNPMGGDPL
LDQAFYGADDFFFPPEGCDNNMLYPQTATSWNVLPPQAMQPAPTYVGRPYTPNYRSTPGS
AMFPYMQSSNSMQWNTAVSPYSSRAPSTTAKNYPPSTFYSQNINQYPRRRTVGMKSSQGN
VPTGNKQSVGKSAKISKPLHIKTSAYQKQYKINLETKARPSAGDEDSAHPDKNKEISMPT
PDSNTLVVQSEEGGAHSLEVDTNRRSDKNLPDAT*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.34677
Recommended expression in:
Not Applicable