VC0419 (V. cholerae )
In pSB3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  VC0419
Gene Name:  ribonuclease G
Original Clone ID:
2880c1TCt1b1
Keyword:
full-length cds
Species:
Vibrio cholerae
Type:
cDNA
Vector Name:
pSB3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
VcCD00534204
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1497nts
Open reading frame: 1 to 1497

AGTGCAGAGTTGCTGTTAAACGTCACCCCGAGTGAAACTCGGGTGGCGATGATTGAAGGG
GGAATCCTTCAGGAAATCCATATTGAACGTGAAGCCAAACGCGGCATTGTTGGCAATATT
TACAAAGGCAAAGTCAGCCGGGTATTACCGGGCATGCAAGCTGCTTTTGTGGATATTGGA
CTCGACAAGGCCGCTTTTTTACATGCCTCGGATATTGTGCCCCACACCGAGTGTGTTGCC
GAAAGTGAGAAGCAGCAGTTCCAAGTGCGCGATATTTCCGAGCTGGTGCGCCAAGGGCAA
GATATTGTGGTGCAAGTGGTCAAAGACCCGCTTGGTACTAAGGGTGCTCGCTTAACTACC
GATATTACGCTCCCTTCTCGTTATCTGGTGTTTATGCCGGGGGCAAGCCATGTTGGCGTA
TCACAACGCATCGAAAGCGAAGCGGAGCGCGAGCGCCTAAAGAAAATCGTTTCTCATTAT
TGCGATGAGCATGGCGGGTTCATTATCCGAACCGCAGCAGAAGGGGCAAACGAAAAAGAG
TTGACTCAAGATGCGGCGTTTCTTAAGCGTTTGTGGTCAAAAGTGATTGAGCGGCGCAGC
AAATACAAGAACCGCTCTATGCTGTATGGCGAGCTTGGTCTGGCGCAGCGCATTTTGCGT
GACTTTGTCGGCACCGAACTGACCAAAATTCTCATCGATTCACGACAAGAGTTCGAAAAC
CTCAAAGAGTTTACCTCCGAATATGTCCCTGAGCTAACGGCTAAATTAGAGCTGTACCAC
GGCGATAAGCCCATTTTTGACATGTACGATACGGAAAATGAGATTCAGCGCTCTCTCGAA
CGCAAAGTGGAGCTCAAATCGGGGGGGTATCTGATCATCGATCAGACCGAAGCCATGACC
ACGATTGACATCAATACGGGCGCGTTTGTTGGCCGCCGTAATCTCGAAGAGACCATTTTC
AACACCAATATTGAAGCGACTCAAGCCATTGCTCGTCAGCTGCGTCTGCGCAATCTGGGT
GGGATTATCATTATTGACTTTATCGATATGGCGTCGGATGAGCACCGTAAGCGAGTGCTC
ACCTCTTTAGAGACGGCGTTGATGAAAGATCGGGTCAAAACCAACATCAACGGCTTTACT
CAGCTTGGGTTGGTGGAGATGACGCGTAAAAGAACCCGAGAAAGCATTGAACATGTGCTC
TGTTCGACTTGTCCAACGTGTGAAGGGCGAGGCAGCGTGAAAACGGTGGAAACGGTGTGC
TTTGAGATTTTGCGCGAAATTACGCGCGTGAATCGAGCCTACGATGCGGACAAATTCGTG
GTTTATGCTTCACCGGCAGTGGCCGATACTTTGCAAGGTGAAGAGTCACATGCCTTGGCG
GAGTTGGAAGTGTTCATTGGTAAAGAAGTGCGAATTCAAGCAGAACCACTCTACAACCAA
GAGCAATTTGATGTTGTGATGATGGAAGGGGGATCCCACCACCACCACCACCACTGAGAT
CGGAAT

Protein sequence predicted based on nucleotide sequence

SAELLLNVTPSETRVAMIEGGILQEIHIEREAKRGIVGNIYKGKVSRVLPGMQAAFVDIG
LDKAAFLHASDIVPHTECVAESEKQQFQVRDISELVRQGQDIVVQVVKDPLGTKGARLTT
DITLPSRYLVFMPGASHVGVSQRIESEAERERLKKIVSHYCDEHGGFIIRTAAEGANEKE
LTQDAAFLKRLWSKVIERRSKYKNRSMLYGELGLAQRILRDFVGTELTKILIDSRQEFEN
LKEFTSEYVPELTAKLELYHGDKPIFDMYDTENEIQRSLERKVELKSGGYLIIDQTEAMT
TIDINTGAFVGRRNLEETIFNTNIEATQAIARQLRLRNLGGIIIIDFIDMASDEHRKRVL
TSLETALMKDRVKTNINGFTQLGLVEMTRKRTRESIEHVLCSTCPTCEGRGSVKTVETVC
FEILREITRVNRAYDADKFVVYASPAVADTLQGEESHALAELEVFIGKEVRIQAEPLYNQ
EQFDVVMMEGGSHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a1c,g2c,S1P;c5t,a6t,A2V;
g7t,E3*
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-2880c
Recommended expression in:
Not Applicable