DNASU Plasmid | Q88KM0 (P. putida KT2440 )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol:

Gene Name:

Original Clone ID:

11062a1BCt5pi1

Keyword:

full-length cds

Species:

Pseudomonas putida KT2440

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

PdCD00534223

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1641nts

Open reading frame: 1 to 1641

AATCGAAAGCATGAAACAGTGAGTGAGTTCGTGCGCCACCTGCCCTGTGAAAACTGTGGT
TCAAGTGATGCCAACTCTATCTATACCGACAACCACCAATATTGCTTCGCCTGTGAGCAC
TACGTTCACGGTGATGGCGAAACCCCAACTACTGGAGGGCGAACAAAACGAATGGAAGGT
TTAATCTCCGGCGAATTCAAGCCGCTGATGAAGCGCAAACTTACCCTGGAAACTTGCCGC
AAGTTCGGATACTTCGTAAGTGAGGTGCGTGGCAGGCTAGTTCAAGTTGCCCCTTACTTC
GACAACAGTGGTGTGATGGTCGCCCAAAAACTGCGCGATCAGGATAAAGGCTTCGCCATC
CTGGGCGATGGTGCAAAGTTGACCCTCTTCGGCCAGAACCTTTGGGCGAGTGGGGGCAAG
AAGATTGTTGTCACCGAAGGCGAGCTGGACGCTATGTCTGTCTCCCAGGTGCAAAACAAC
AAGTGGCCCGTGGTGTCGCTCCCGAACGGGGCTCCGGCAGCACGCAAGGCCATCCAGCGA
AACATCGAATATCTGGAGAGCTTCGAAGAAGTCATCCTCATGTTCGATATGGACGAACCG
GGCCGCGAAGCAGCGCAGGAATGCGCAGAGCTATTCAGCCCAGGCAAGTGCAAGATCGCC
ACGCTATCCATGAAGGACGCCAACGAACTTCTGGTAGCGGGCCGTGAGCAGGAAATCGTC
ACCGCCATCTGGAACGCCAAGCTGTACCGGCCTGATGGTGTAGTGAACGTCCGAGACCTC
CTCGAAGAGATTCGCAAGGCCCTGGTCATGGGCTTGCCGTGGTTCCTTGATCCACTCACC
CAACTCACCTATGGACGCCGCTACGGCGAAGTCTACGGGCTGGGCGCTGGTACTGGTGTG
GGCAAGACCGACTTCCTCACGCAGCAAATCGCATACGACATCCAAGTCCTGGGCGAGCGT
GTAGGCACCATTTTCCTGGAGCAGAAACCGACCGAGACCGCCAAGCGCGTGGCCGGGAAG
ATCGCCGGGAAACGCTTCCACGTTCCCAAGGACACCGCTGGCTGGACCGATGAGGAGCTG
GATGCAGCCGTAGATGCGCTGGGCGAAAACCTGGTCATGTACGACGCATTTGGTGAAACC
GAGTGGGACATTGTGAAGCGCAAGGTGCGCTACATGGCTGTCTCCGAGGGCATCAAGCTT
ATCTACATTGACCACCTCACTGCGATGGCTGACACCGCAGATGAGAAGGGCTCGCTTGAG
CAGATCATGAAAGAGATGGCGGGCCTGGCGAACGAGCTGGGAATCATCATCACGTTCATC
TCTCACCTGACCACCCCCGAGGGCAAACCCCATGAGGAAGGCGGGCGGGTAACGATTCGC
CACTTCAAAGGCTCCCGAGCCATCGGCTTCTGGAGCTACTTCATGTTCGGCCTTGAGCGT
GACCAACAGGCCGAAGACCCCGTGGTTCGCCAAACCACCACCTTCCGCATCCTCAAGGAC
CGCTGCACCGGCCAAGCGACCGGGGAAGTGCTGTACCTGGCATACGACCGCGACACCGGA
TTGCTCTCGCTCACCGAAGCGCCCGAGCCGTCCTCACCCTTTAAAGATGAATCCGAAGGG
CATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

NRKHETVSEFVRHLPCENCGSSDANSIYTDNHQYCFACEHYVHGDGETPTTGGRTKRMEG
LISGEFKPLMKRKLTLETCRKFGYFVSEVRGRLVQVAPYFDNSGVMVAQKLRDQDKGFAI
LGDGAKLTLFGQNLWASGGKKIVVTEGELDAMSVSQVQNNKWPVVSLPNGAPAARKAIQR
NIEYLESFEEVILMFDMDEPGREAAQECAELFSPGKCKIATLSMKDANELLVAGREQEIV
TAIWNAKLYRPDGVVNVRDLLEEIRKALVMGLPWFLDPLTQLTYGRRYGEVYGLGAGTGV
GKTDFLTQQIAYDIQVLGERVGTIFLEQKPTETAKRVAGKIAGKRFHVPKDTAGWTDEEL
DAAVDALGENLVMYDAFGETEWDIVKRKVRYMAVSEGIKLIYIDHLTAMADTADEKGSLE
QIMKEMAGLANELGIIITFISHLTTPEGKPHEEGGRVTIRHFKGSRAIGFWSYFMFGLER
DQQAEDPVVRQTTTFRILKDRCTGQATGEVLYLAYDRDTGLLSLTEAPEPSSPFKDESEG
HHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

Y506C

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in NYSGXRC-11062a

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

GenBank Accession :

AE015451

Target GenBank:

AE015451

Protein

SMART domain :

Prim_Zn_Ribbon : Zinc-binding domain of primase-helicase

SMART domain :

TOPRIM :

Structural Annotation Wiki :

NYSGXRC-11062a

Clone

Cloning Information :

480064

HIP Master Clone ID :

397957

Original Clone ID :

11062a1BCt5pi1

Experimental

TargetTrack - Experimental data :

NYSGXRC-11062a

Q88KM0 (P. putida KT2440 )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Q88KM0 (P. putida KT2440 )
In pSGX3 (His-tagged bacterial expression vector)