Gene:
Gene Symbol:
IL2005
Gene Name:
Na+/proline symporter
Original Clone ID:
GE.541.1.29_GO.13369
Keyword:
None
Species:
Idiomarina loihiensis L2TR
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format:
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
New York Consortium on Membrane Protein Structure
IlCD00534236
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 3474nts
Open reading frame: 51 to 3524
ACCATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGACCTGGT
TACTGATATTTGCCTCGCTGGCTTATGTGATTTTCCTCTTCGCAGTTGCCACCTGGGGCG
ACCGTTTAGGCTCGTGGGCGAATAAAATCAGTCACAAGCCAGTTGTCTACTCTATGTCGC
TGGCCATCTACTGCACGTCCTGGACGTATTACGGCGCTGTGGGTAATGCAGCAGTTGACG
GTTGGGCGTTCTTTCCTATTTTGCTCGGGCCAATTTTGCTGTTCTTGTTTGGTATCCGAA
TTCTGGAAAAGCTGGTTTCGGTTAGTAAACAACAAAATATTACTTCAATAGCCGACTTTA
TTTCTTCCCGCTTTGGTAAACGCCGCCGGCTTGCCGTGGCTGTTACCGCCATTGCTGCCG
TTGCTATTATTCCTTACATTGCGCTTCAGTTAAAAGCTGTGGGCATTAGCTTTGCGGTTC
TGACTGAAAGCTCTGGAGTGATGGCACAGTTTTCGATAAAAGAACTGGGTGCTGCTGCGC
TAATGGCAATTTTTGCTATGTTGTTTGGTACCCGCCACGTCGAAGTCACTGAATATCGCA
GTGGCATGATACTGGCTATTGCTGTTGAGTCGATGATCAAACTGGTTGCATTGCTGGCAG
CTGGTGTTTTTGCCTGGTTCCTGATTCAGGGAGACGCGCAATCGGACTTATTCAGTTCAT
TCAGTGACGCAACGAAACCTCAGTGGTCATTTGCAGCCTTTGGCGAGTTTGAGTTCATTG
TACAAACCCTGATGGCGGCCGGGGCAATTTTGTGTTTACCCAGACAGTTTCATGTGGCGG
TAGTGGACAATGTTGATGTCCGGCATTTACGCACGGCTCGTTGGGGCTTTCCTCTGTATC
TGGCGCTGACCGCTGCCGCAATTGTCCCCATTGCTATTGCAGGAACTTTCTATTTGTCAG
CAACCACCGACGGTTCAACGTTTGCGCTGCAACTGCCGTTACTCAAAGACCAGCTTTGGT
TATCACTGGTTATTTTTATTGGTGGTATATCCGCAGCAACAGCCATGGTGATTATCGCGT
CCGTAACCTTAAGCACCATGATAACTAACGACGTTATCATGCCCATTTTGCTGCAGCGCG
AGTCGCGCTTGCCGCTAAAACATCGGCGTCGTGAATACGACCGGGCCTTACTATTAATTC
GGCGTGTGGCTATTGCCGTTACTATGGTGCTGTCGTTCTTGTGTTATTACTTCTGGGCAG
CCCGGACTAACCTGGTCAGTATTGGTTTGTTGGCGTTCTCGTTGGTGTTGCAGTTGCTGC
CGGCGTTGCTGGCCGCTCTGTATTGGCGTAAAGGCCACGCGCGGGGTGTTTACGCCGGGT
TGGCTGCAGGTTCAGTCGGCTGGGTTTTAGCGGTACTAATACCACTTTACACCGACTTGC
AACTGACCGATGTGGAAATAATAACCCGCGGTACTGTGGTTTCTTTAGCCTTAAATTGCT
TCGGGTTCATTGTATTTTCATTGTTGGCAAAACCACGTCTGGTTGATCGTATTCAGGCAA
CGGCTTTTGTTAAACCCCGAGCCGCTTTACCCGATTTGCATTTCAACAAAAACGACAGTG
CTACCAATGCCGATATGTTGTTGCTGTTAAGGACCTTTTTAGGGGCGGAACGGGCTCAGC
AGTTAATGCAGTATTATCAGCGTCCGGAAAACACTGAGCTTCCGCTGGAAGGTGTGGCTG
AAAAAACCTTTATCAGTTTTGTGGAAAGAGCGATAGCTGGTGTACTTGGCTCTGCCACGG
CGCGCTCTCTTGTCGATGCTGCACTGCGCGATAAGCAGCTAAATTTAGAAGAAGTTGTTC
ACTTCTTTGACGATACAACGCAAGCTCTGCAAACTCAGCAATCTATTTTGTTTAGCTCAC
TGGAGAACCTGGCTCAGGGCATTAGTGTTGTTGACGCGGAGCTGAGGCTGGTGGCCTGGA
ATAAACGCTATATGGATTTATTCCAGTACCCTGAGGGCATGGTGAAACCCGGACAGCCCA
TAGCGACCCTTATTCGTTATAACGCAGAGCGCGGAGAATGTGGTCCCGGTGACGTTGACG
GGTTGGTGAATAAGCGTTTGCGCTATATGCAGCTGGGATCGGCACACCGGTTTATTCGCC
GGCGCGCCGATGGCCGGGTTATAGAAATGGTAGGTAACCCTTTGCCGACCGGAGGCTTCG
TTACCAGCTTTACCGACATTACTTCGCACGTTGAAACCCAGCAGGCACTGGAAGATGCCA
ATATTGATCTGGAACAGCGGGTTGACGTTCGGACCCAGGAAATACGCGAGATAAACCAGG
AGCTGACAGCGGAAATAGACAGGCGCCGGCAAGTTGAGGACGAGCTGAAAAAAGCCAAGG
CTGAAGCGGAAGCGACTAATGCTTCTAAAACACGTTTTCTGGCGTTGGCCAGTCATGATA
TTTTGCAGCCTCTTAATGCGGCGCGGTTGTACCTTTCGGCTATTCAGGAGAAAGAACTTT
CGGAGCGGAATTTGTCTCTGGTTGGCAAGCTGGACACCGCGCTTGGTTCAACAGAGCATT
TACTCTCTACTTTATTGTCGATAGCAAAAATGGATCAGGGGGCTTTAAAACCGCAGTTCC
GGCATGTGCAACTTAGCTCATTATTGGAGCCTTTGGTTGAAGAATATGCAGTGCTGGCAG
AGCAACGAAAGCTGGATTTTCGTGCGTTCTATAAAGACCGGCCGGTGTATACCGACCCAA
CGTATTTGCGCCGAATTGTGCAGAACTTTTTGTCTAATGCCATAAAATACACCCAAAGGG
GCAGCGTGCTGTTGGGCGTGCGCAAACGCGGAGATGAGCTTCAGATAGCCGTGTGGGATA
CCGGGCCGGGCATTGCAGCGCAGCAGCAAAATAAGGTATTTGATGCATTTTATCGAGTGC
AGAACCAAACCGTAGACGGTGTCGGCCTTGGATTAAGTGTCGCGCAACGTATGGGTGAGC
AGTTAAACTGTGACGTACAGGTTGAGTCGGTTGAAGGTAAAGGCTCGTGTTTTTCGGTAC
GAGTGCCGTTAGGCAATGCTGCTCAGGTTGTCAGCCGACCGAAAAAACAACTGGAAGATG
CTTTATCTGATCCTTTACAGCTTATTGTGGTCGATGACGACAATGAAAATTTAAGTGCGT
TGAGTGCGCTGCTGAATAAGTGGGGGTGCGAAGCCCTGACATTTAATCGTTATAGCAAAG
CTTTAGCCTATGCTAAGGCTCACAGCCAGAACAAAGCGCCGCACGGACTGTTATTAGATT
ACCAGTTAGGTGACGGTGACGGCTTGCAGTTGGCGCAGGAACTACGTGATATTTGGCAGC
AACAGATACCGGCTGCTTTGGTGACAGCGATGCGTGACGATGCCGTGAAGCAGGCGGCCC
GCAAAAGCGGGCTGCAGTTTTTACCTAAACCCGCTAAACCTGCCGCATTAAAAGCATTTT
TAAAATACATTAGTACCCGAAAGGCGGGAGGAGCCAGAAGTTAGCCCGTATAATATTGAG
GGCTCGAGCACCACCACCACCACCACTGA
Protein sequence predicted based on nucleotide sequence
MTWLLIFASLAYVIFLFAVATWGDRLGSWANKISHKPVVYSMSLAIYCTSWTYYGAVGNA AVDGWAFFPILLGPILLFLFGIRILEKLVSVSKQQNITSIADFISSRFGKRRRLAVAVTA IAAVAIIPYIALQLKAVGISFAVLTESSGVMAQFSIKELGAAALMAIFAMLFGTRHVEVT EYRSGMILAIAVESMIKLVALLAAGVFAWFLIQGDAQSDLFSSFSDATKPQWSFAAFGEF EFIVQTLMAAGAILCLPRQFHVAVVDNVDVRHLRTARWGFPLYLALTAAAIVPIAIAGTF YLSATTDGSTFALQLPLLKDQLWLSLVIFIGGISAATAMVIIASVTLSTMITNDVIMPIL LQRESRLPLKHRRREYDRALLLIRRVAIAVTMVLSFLCYYFWAARTNLVSIGLLAFSLVL QLLPALLAALYWRKGHARGVYAGLAAGSVGWVLAVLIPLYTDLQLTDVEIITRGTVVSLA LNCFGFIVFSLLAKPRLVDRIQATAFVKPRAALPDLHFNKNDSATNADMLLLLRTFLGAE RAQQLMQYYQRPENTELPLEGVAEKTFISFVERAIAGVLGSATARSLVDAALRDKQLNLE EVVHFFDDTTQALQTQQSILFSSLENLAQGISVVDAELRLVAWNKRYMDLFQYPEGMVKP GQPIATLIRYNAERGECGPGDVDGLVNKRLRYMQLGSAHRFIRRRADGRVIEMVGNPLPT GGFVTSFTDITSHVETQQALEDANIDLEQRVDVRTQEIREINQELTAEIDRRRQVEDELK KAKAEAEATNASKTRFLALASHDILQPLNAARLYLSAIQEKELSERNLSLVGKLDTALGS TEHLLSTLLSIAKMDQGALKPQFRHVQLSSLLEPLVEEYAVLAEQRKLDFRAFYKDRPVY TDPTYLRRIVQNFLSNAIKYTQRGSVLLGVRKRGDELQIAVWDTGPGIAAQQQNKVFDAF YRVQNQTVDGVGLGLSVAQRMGEQLNCDVQVESVEGKGSCFSVRVPLGNAAQVVSRPKKQ LEDALSDPLQLIVVDDDNENLSALSALLNKWGCEALTFNRYSKALAYAKAHSQNKAPHGL LLDYQLGDGDGLQLAQELRDIWQQQIPAALVTAMRDDAVKQAARKSGLQFLPKPAKPAAL KAFLKYISTRKAGGARS*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.13369
Recommended expression in:
Not Applicable
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments:
None
Size (bp):
6984
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
New York Consortium on Membrane Protein Structure
New York Structural Biology Center
New York Structural Biology Center
Publications:
PMID:
20394823
Title: High-throughput expression and purification of membrane proteins.
Vector Map:
.jpg)
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1583
2265
death cassette
ccdB
ccdB death cassette (removed in plasmids containing an insert)
5393
5602
promoter
T7
T7 promoter
5146
5165
protease cleavage site
TEV
TEV protease cleavage site
5290
5310
repressor protein gene
LacI
Lac inhibitor
3677
4693
ribosome binding site
RBS
Ribosome Binding Site
5225
5230
selectable marker
KanR
kanamycin resistance
725
1537
selectable marker
CmR
Chloramphenicol resistance gene
6040
6696
tag
Flag
Flag epitope tag; N terminal
5236
5259
tag
His
10xHis tag; N terminal
5260
5289
trxn regulatory element
lac operator
lac operator
5165
5187
trxn termination sequence
T7 term
T7 terminator
91
137
