CPSF100 (A. thaliana Columbia )
In pDONR221 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  CPSF100
Gene Name:  cleavage and polyadenylation specificity factor 100
Original Clone ID:
5566.1.34_GO.29756
Keyword:
full-length cds
Species:
Arabidopsis thaliana Columbia
Type:
cDNA
Vector Name:
pDONR221
Format: 
CLOSED
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Wisconsin Madison
PSI
Center for Eukaryotic Structural Genomics
AtCD00534293
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 2238nts
Open reading frame: 51 to 2288

ATGAGCAATGCTTTTTTATAATGCCAACTTTGTACAAAAAAGCAGGCTCCGAAAACCTGT
ACTTCCAGTCCGGTACTTCGGTGCAAGTGACTCCTCTCTGCGGAGTTTACAACGAGAATC
CTCTCTCCTACTTAGTCTCCATTGATGGTTTCAACTTCCTCATCGACTGTGGTTGGAACG
ATCTCTTCGACACATCGCTCCTCGAACCTCTCTCCAGGGTTGCTTCTACTATAGATGCAG
TTTTGCTTTCTCATCCAGATACACTTCACATTGGTGCTCTTCCTTATGCTATGAAGCAGC
TTGGACTCTCCGCTCCTGTTTATGCTACTGAGCCTGTTCATAGATTAGGTCTCCTTACAA
TGTATGATCAGTTTTTGTCCAGGAAGCAAGTATCCGACTTTGATCTGTTTACACTGGATG
ACATAGATTCTGCTTTCCAGAATGTGATCAGATTGACTTACTCTCAAAATTACCATCTTT
CTGGAAAGGGAGAGGGTATTGTAATTGCTCCTCATGTTGCTGGACACATGCTGGGAGGTA
GCATCTGGAGGATAACAAAGGATGGGGAGGATGTTATATATGCTGTTGACTACAATCATC
GGAAAGAAAGGCATTTGAATGGAACTGTTTTACAGTCTTTTGTTCGGCCTGCTGTTCTGA
TAACCGATGCATATCATGCTCTTTATACCAATCAAACCGCAAGACAGCAAAGGGACAAAG
AATTTCTAGATACCATTTCAAAACATCTTGAAGTTGGAGGAAATGTTCTATTGCCAGTAG
ACACTGCGGGTCGTGTCCTGGAACTTCTCTTGATACTTGAACAGCATTGGTCACAAAGAG
GTTTCAGCTTTCCCATTTATTTTCTCACGTACGTGTCATCTAGCACAATTGACTATGTTA
AGAGTTTCCTCGAGTGGATGAGTGACTCCATTTCAAAGTCCTTTGAGACTTCACGTGATA
ATGCCTTTCTATTGAGGCATGTCACTCTGTTGATAAACAAGACTGATCTGGATAATGCTC
CACCTGGTCCAAAGGTTGTTCTTGCTTCCATGGCTAGTCTCGAAGCCGGTTTTGCTCGAG
AGATATTCGTGGAGTGGGCAAATGATCCCAGAAATTTAGTCCTCTTTACTGAAACAGGCC
AGTTTGGCACTTTAGCTCGTATGCTTCAGTCAGCCCCACCTCCAAAATTTGTTAAAGTCA
CCATGTCTAAGAGGGTTCCTTTGGCTGGGGAAGAGTTAATTGCATATGAGGAAGAGCAAA
ACAGACTGAAAAGGGAAGAAGCTTTGCGAGCTAGCCTCGTTAAAGAGGAGGAAACAAAAG
CTTCCCATGGATCCGATGATAATTCAAGTGAACCTATGATCATAGATACCAAGACTACTC
ACGATGTTATTGGTTCTCACGGGCCTGCATATAAAGATATATTGATTGATGGATTTGTTC
CCCCATCCAGCAGCGTAGCTCCAATGTTCCCATATTATGATAACACATCTGAATGGGACG
ACTTTGGGGAGATTATTAATCCAGATGACTATGTGATCAAGGATGAAGACATGGACCGAG
GAGCAATGCATAACGGAGGTGATGTGGACGGAAGGCTTGATGAGGCAACTGCTAGTCTCA
TGCTTGATACAAGACCTTCGAAAGTCATGTCCAATGAGCTCATTGTGACTGTTAGTTGTT
CACTTGTTAAAATGGACTATGAAGGTCGGTCAGATGGCCGCTCAATCAAGTCAATGATTG
CGCATGTTTCTCCTTTAAAACTTGTTTTGGTGCACGCGATAGCTGAGGCTACAGAGCATT
TGAAGCAACACTGCTTGAACAACATCTGTCCACACGTGTATGCTCCTCAAATAGAGGAAA
CGGTCGATGTGACTTCTGATTTATGTGCTTACAAGGTCCAACTTTCTGAAAAGCTGATGA
GCAATGTGATCTTCAAGAAGCTGGGAGATTCAGAAGTAGCTTGGGTGGATTCCGAAGTAG
GGAAGACAGAGAGGGACATGAGGTCTCTACTACCGATGCCAGGTGCTGCTTCGCCACACA
AACCTGTTCTAGTAGGTGATCTGAAAATCGCAGACTTCAAGCAGTTTCTGTCGAGCAAGG
GTGTTCAGGTAGAATTTGCAGGTGGAGGAGCTTTACGTTGTGGTGAATATGTCACTCTAC
GAAAGGTTGGACCGACGGGTCAAAAGGGAGGAGCATCGGGTCCACAGCAAATTCTGATAG
AAGGACCGTTGTGTGAAGACTATTACAAAATCAGGGATTATCTCTATTCTCAGTTCTACC
TCCTCTGATAGGACC

Protein sequence predicted based on nucleotide sequence

ENLYFQSGTSVQVTPLCGVYNENPLSYLVSIDGFNFLIDCGWNDLFDTSLLEPLSRVAST
IDAVLLSHPDTLHIGALPYAMKQLGLSAPVYATEPVHRLGLLTMYDQFLSRKQVSDFDLF
TLDDIDSAFQNVIRLTYSQNYHLSGKGEGIVIAPHVAGHMLGGSIWRITKDGEDVIYAVD
YNHRKERHLNGTVLQSFVRPAVLITDAYHALYTNQTARQQRDKEFLDTISKHLEVGGNVL
LPVDTAGRVLELLLILEQHWSQRGFSFPIYFLTYVSSSTIDYVKSFLEWMSDSISKSFET
SRDNAFLLRHVTLLINKTDLDNAPPGPKVVLASMASLEAGFAREIFVEWANDPRNLVLFT
ETGQFGTLARMLQSAPPPKFVKVTMSKRVPLAGEELIAYEEEQNRLKREEALRASLVKEE
ETKASHGSDDNSSEPMIIDTKTTHDVIGSHGPAYKDILIDGFVPPSSSVAPMFPYYDNTS
EWDDFGEIINPDDYVIKDEDMDRGAMHNGGDVDGRLDEATASLMLDTRPSKVMSNELIVT
VSCSLVKMDYEGRSDGRSIKSMIAHVSPLKLVLVHAIAEATEHLKQHCLNNICPHVYAPQ
IEETVDVTSDLCAYKVQLSEKLMSNVIFKKLGDSEVAWVDSEVGKTERDMRSLLPMPGAA
SPHKPVLVGDLKIADFKQFLSSKGVQVEFAGGGALRCGEYVTLRKVGPTGQKGGASGPQQ
ILIEGPLCEDYYKIRDYLYSQFYLL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in GO.29756
Recommended expression in:
Not Applicable