DNASU Plasmid | gi|82746774:c61794-60595 (C. beijerinckii NCIMB 8052 )<br>In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol:

Gene Name:

Original Clone ID:

9459d2BCt11p1

Keyword:

partial length cds

Species:

Clostridium beijerinckii NCIMB 8052

Type:

cDNA

Vector Name:

pSGX3

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

Yes / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

CbCD00534300

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1281nts

Open reading frame: 51 to 1331

TCCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTGAACCGACCA
TTATCACGGATGTGCTGTGCTATATTACTAAACCAGACCGTCATAATCTGGTGGTAGTTA
AGGTGGAGACAAATAAGGGGATCTACGGTCTGGGGTGTGCGACCTTTCAGCAACGCCCGA
AAGCCGTTAGCCTGGTCGTATCCGAGTACCTGAAACCAATTCTGATCGGTCGCGATGCAA
ACAACATTGAGGACCTGTGGCAGATGATGATGGTGAATAGTTATTGGCGCAATGGTCCGA
TCCTGAATAACGCTATTTCAGGTGTTGACATGGCGCTGTGGGACATCAAAGGAAAGCTGG
CTAATATGCCGCTGTATCAACTGTTCGGTGGCAAGTCACGTGACGCCATTGCCGCGTACA
CTCACGCTGTAGCAGATAACCTGGAGGACCTGTATACTGAAATCGACGAGATCCGCAAGA
AAGGGTACCAGCATATCCGTTGCCAGCTGGGGTTTTACGGCGGTAATTCGAGCGAGTTCC
ATACTACGGACAACCCGACGCAAGGCTCTTATTTTGATCAGGACGAGTACATGCGCACCA
CTGTGTCTATGTTTTCGTCCCTGCGTGAAAAGTACGGTTACAAATTTCATATTCTGCACG
ACGTACACGAACGCCTGTTCCCGAATCAAGCTGTACAGTTTGCGAAAGACGTGGAGAAGT
ATAAACCGTACTTCATCGAGGACATTCTGCCTCCGGATCAGAACGAGTGGCTGGGGCAAA
TTCGTTCGCAGACTTCCACCCCACTGGCCACCGGCGAACTGTTTAATAACCCGATGGAAT
GGAAATCCCTGATTGCGAATCGCCAAGTCGACTTTATTCGTTGTCACGTTAGTCAGATCG
GTGGGATTACTCCGGCCCTGAAACTGGGCTCACTGTGCGCGGCCTTTGGAGTACGTATCG
CATGGCATACACCGTCCGATATTACCCCGATCGGCGTGGCGGTCAATATCCACCTGAATA
TTAATCTGCATAATGCTGCTATTCAGGAAAATATCGAAATTAATGATAACACGCGTTGTG
TTTTTTCGGGTATCCCGGAAGCAAAGAACGGGTTCTTTTATCCGATTGAAAGCCCGGGCA
TCGGTGTGGACATTGATGAAAACGAGATCATTAAATACCCGGTAGAACATCGCCCGCACG
AGTGGACGCAGTCTCGTATTCCGGACGGCACTATCGTCACAAAGGGCATCATCACCATCA
CCACTGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACC
ACCACCACTGAGATCCGG

Protein sequence predicted based on nucleotide sequence

EPTIITDVLCYITKPDRHNLVVVKVETNKGIYGLGCATFQQRPKAVSLVVSEYLKPILIG
RDANNIEDLWQMMMVNSYWRNGPILNNAISGVDMALWDIKGKLANMPLYQLFGGKSRDAI
AAYTHAVADNLEDLYTEIDEIRKKGYQHIRCQLGFYGGNSSEFHTTDNPTQGSYFDQDEY
MRTTVSMFSSLREKYGYKFHILHDVHERLFPNQAVQFAKDVEKYKPYFIEDILPPDQNEW
LGQIRSQTSTPLATGELFNNPMEWKSLIANRQVDFIRCHVSQIGGITPALKLGSLCAAFG
VRIAWHTPSDITPIGVAVNIHLNINLHNAAIQENIEINDNTRCVFSGIPEAKNGFFYPIE
SPGIGVDIDENEIIKYPVEHRPHEWTQSRIPDGTIVTKGIITITTDPNSSSVDKLAAALE
HHHHHH*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

Y381H

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in NYSGXRC-9459d

Recommended expression in:

Not Applicable

Vector Name:

pSGX3

Synonyms:

pSGX-3, pSGX3_BC

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen

Size (bp):

5339

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAAGGGCATCATCACCATCACCACTGATCCCACCACCACCACCACCACTGAGATCCGAATTCGAGCTCCGTCGACAAGCTTGCGGCCGCACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

ATG start

start

initiation start (Met, Ser, Leu)

5072

5079

TOPO recognition site

TOPO

TOPO cloning site

5076

5085

bacterial origin

ori

pBR322-type bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4983

5003

repressor protein gene

LacI

LacI CDS

3515

4531

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

His

6xHis tag

5086

5103

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 terminator

5231

5307

Gene

GenBank Accession :

82746826

Target GenBank:

None

Protein

SMART domain :

MR_MLE : Mandelate racemase / muconate lactonizing enzyme, C-terminal domain

Structural Annotation Wiki :

NYSGXRC-9459d

Clone

Cloning Information :

480481

HIP Master Clone ID :

398374

Original Clone ID :

9459d2BCt11p1

Experimental

TargetTrack - Experimental data :

NYSGXRC-9459d

gi|82746774:c61794-60595 (C. beijerinckii NCIMB 8052 )In pSGX3 (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

gi|82746774:c61794-60595 (C. beijerinckii NCIMB 8052 )
In pSGX3 (His-tagged bacterial expression vector)