leuA (C. acetobutylicum ATCC 824 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  leuA
Gene Name:  alpha-isopropylmalate synthase
Original Clone ID:
11104h2BCt9p1
Keyword:
full-length cds
Species:
Clostridium acetobutylicum ATCC 824
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
CaCD00534301
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1647nts
Open reading frame: 1 to 1647

GGCAAGGAAAAGAAGGTGTACATTTTTGATAGTATACTTAGAGACGGTTCTCAAGCACAA
GGTGTTTCCTTTAGTGTGGCTGATAAGATTAAAATAGTAAAAAAGTTAGATGAACTGGGA
ATTGACTATATAGAAGCTGGAAATCCTAGCTCAAACCCAAAAGATAGAGAATTCTTTGAA
GAAATAAGAAGTTTAAAGCTTAAACATTCTAAGTTGTGTGCATTTGGAAGTACTAGAAGA
GCAGGTGCAAAGGCTGAGGAAGATGAAGGAATAAAAAAACTCGTAGATAGTGGAGTAAAT
GTATTCTCTATATTTGGTAAGGCATGGGACTTTCATGTTACAGATATAATAAAAACCTCT
ATTGAAGAAAATTTAAACATGATTAGAGATAGCATAAAATACTTAGTTTCTTTGAATAAG
GAAGTTGTATTTGACGCAGAACACTTCTTTGATGGTTTTAAAAGCAATAAAGAATATGCC
ATAGAAGTTTTAAATACTGCGAGATTAGCAGGAGCAAAATGGATTGTGCTTTGTGACACC
AATGGAGGAACTATGCCAAATGAAGTGAGTGACATAGTCAAGTACTTAGTAAGGGATTTA
AACTTTGATAATTTGGGAGTACATTTTCATGATGACAGCGGCATGGCAGTAGCCAATTCA
GTTGTAGCAGTGCATTATGGAGTTAATCATGTTCAAGGAACCTTTACAGGTGTAGGAGAA
AGATGTGGAAATGCTAATTTATCAAACATAATAGCAAATCTAAGCCTTAAAATGGGCTAT
AATACCATAAAAAATGATAAGCTTGAATATTTGACAGTGGCTTTTAGATATATATGTGAA
GCCTGTAATATGACACCTAATGAGAGGGTTGCTTATGTTGGAAATTGTGCTTTCTCTCAT
AAAGGTGGTATGCATATAGATGCTGTAAGAAAGAATCCAAAGTCCTTTGAACACATAGAT
CCATCTAGGGTTGGAAACTCAAGAAGAATACTTGTATCTGAGGTATCTGGAAGGAGTACA
ATACTTCCAGCTTTAAAAAGGATAAATCCTAAAATAGACAAGGATTCTCCCGAAACAAAG
GCAGTAGTTAAAAGAATAAAGGACTTAGAGTATCTTGGATATCAATTTGAAGGAGCAGAA
GGAAGTTTTGAGCTTCTTATGAGAAAAGAACTTAACGTATTTAGACCATCTTTTGAACTT
GTAGAGTATAAGGTAATTGCTGAAAGGCCATATCTAAGTGACTTAAGTGCTACAGCAATA
GTTAAAATAATGGTTAATGGCAAACTTGAAACAACCGCAGCAGATGGTAAAGGACCTGTA
AACGCACTTGATAAAGCTTTAAGAAAAGCACTTGGAGTGTTCTACAAAGAATTAGCAAAT
GTTTATCTTACAGACTATAAGGTAAGAGTAATCAGTGGAACTAAGGCTACAAGTGCAAAA
ATAAGAGTATTAATTGAGTCTACAGATGGTGTATCAACATGGACAACAGTTGGTGTTTCC
TATGACATAATAGAAGCCAGCTGGCTAGCACTTGTAGATTCAGTAGAATATAAATTAATA
CAATGCAATAAGGAGGAAAATAACAATGAAAAAACCGATGACTATGACACAAAAAATACT
GAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

GKEKKVYIFDSILRDGSQAQGVSFSVADKIKIVKKLDELGIDYIEAGNPSSNPKDREFFE
EIRSLKLKHSKLCAFGSTRRAGAKAEEDEGIKKLVDSGVNVFSIFGKAWDFHVTDIIKTS
IEENLNMIRDSIKYLVSLNKEVVFDAEHFFDGFKSNKEYAIEVLNTARLAGAKWIVLCDT
NGGTMPNEVSDIVKYLVRDLNFDNLGVHFHDDSGMAVANSVVAVHYGVNHVQGTFTGVGE
RCGNANLSNIIANLSLKMGYNTIKNDKLEYLTVAFRYICEACNMTPNERVAYVGNCAFSH
KGGMHIDAVRKNPKSFEHIDPSRVGNSRRILVSEVSGRSTILPALKRINPKIDKDSPETK
AVVKRIKDLEYLGYQFEGAEGSFELLMRKELNVFRPSFELVEYKVIAERPYLSDLSATAI
VKIMVNGKLETTAADGKGPVNALDKALRKALGVFYKELANVYLTDYKVRVISGTKATSAK
IRVLIESTDGVSTWTTVGVSYDIIEASWLALVDSVEYKLIQCNKEENNNEKTDDYDTKNT
EGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11104h
Recommended expression in:
Not Applicable