ipaH_4 (S. flexneri 2a str. 2457T )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  ipaH_4
Gene Name:  invasion plasmid antigen
Original Clone ID:
13502b2BCt14p1
Keyword:
full-length cds
Species:
Shigella flexneri 2a str. 2457T
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
SfCD00534339
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1767nts
Open reading frame: 1 to 1767

ACAAATAACAATCACAGATTAATTTCAAATTCGTTCTCCACTTATTCAATCGACACTAGC
CGCGCATATGAAAATTATCTAACCCATTGGACTGAATGGAAAAATAACCGCATACAAGAA
GAACAACGAGACATCGCTTTTCAGCGACTAGTATCATGTCTACAAAACCAAGAGACGAAC
CTGGACTTGTCTGAATTAGGCCTGACAACATTACCTGAAATCCCCCCGGGAATTAAATCA
ATTAATATAAGTAAAAATAATTTAAGCTTAATCCCCCCATTGCCTGCGTCCCTTACACAA
CTTAATGTCAGCTATAACAGACTTATTGAACTGCCTGCTTTGCCTCAAGGACTTAAATTA
TTGAATGCGTCCCACAATCAACTAATCACACTACCCACACTCCCCATATCTTTGAAGGAG
CTTCATGTCTCAAATAATCAATTATGTTCTCTTCCTGTTTTACCAGAACTACTGGAAACA
TTAGATGTATCATGTAATGGGCTGGCAGTTTTACCACCTTTACCATTTTCTTTACAAGAG
ATTAGCGCAATAGGGAATCTTCTTAGTGAACTCCCCCCTCTACCTCACAACATTCACTCC
ATATGGGCAATCGACAATATGTTAACCGATATTCCATACCTGCCGGAAAATTTAAGGAAC
GGTTATTTTGACATAAATCAGATAAGTCATATCCCGGAAAGCATTCTTAATCTGAGGAAT
GAATGTTCAATAGATATTAGTGATAACCCATTGTCATCCCATGCTCTGCAATCCCTGCAA
AGATTAACATCTTCGCCGGACTACCACGGCCCGCAGATTTACTTCTCCATGAGTGACGGA
CAACAGAATACACTCCATCGCCCCCTGGCTGATGCCGTGACAGCATGGTTCCCGGAAAAC
AAACAATCTGATGTATCACAGATATGGCATGCTTTTGAACATGAAGAGCACGCCAACACC
TTTTCCGCGTTCCTTGACCGCCTTTCCGATACCGTCTCTGCACGCAATACCTCCGGATTC
CGTGAACAGGTCGCTGCATGGCTGGAAAAACTCAGTGCCTCTGCGGAGCTTCGACAGCAG
TCTTTCGCTGTTGCTGCTGATGCCACTGAAAGCTGTGAGGACCGTGTCGCGCTCACATGG
AACAATCTCCGGAAAACCCTCCTGGTCCATCAGGCATCTGAAGGCCTTTTCGATAATGAT
ACCGGCGCTCTGCTCTCCCTGGGCAGGGAAATGTTCCGCCTCGAAATTCTGGAGGACATT
GCCCGGGATAAAGTCAGAACTCTCCATTTTGTGGATGAGATAGAAGTCTACCTGGCCTTC
CAGACCATGCTCGCAGAGAAACTTCAGCTCTCCACTGCCGTGAAGGAAATGCGTTTCTAT
GGCGTGTCGGGAGTGACAGCAAATGACCTCCGCACTGCCGAAGCCATGGTCAGAAGCCGT
GAAGAGAATGAATTTACGGACTGGTTCTCCCTCTGGGGACCATGGCATGCTGTACTGAAG
CGTACGGAAGCTGACCGCTGGGCGCTGGCAGAAGAGCAGAAATATGAGATGCTGGAGAAT
GAGTACCCTCAGAGGGTGGCTGACCGGCTGAAAGCATCAGGTCTGAGCGGTGATGCGGAT
GCGGAGAGGGAAGCCGGTGCACAGGTGATGCGTGAGACTGAACAGCAGATTTACCGTCAG
CTGACTGACGAGGTACTGGCCCTGCGATTGCCTGAAAACGGCTCACAACTGCACCATTCA
GAAGGGCATCATCACCATCACCACTGA

Protein sequence predicted based on nucleotide sequence

TNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEEQRDIAFQRLVSCLQNQETN
LDLSELGLTTLPEIPPGIKSINISKNNLSLIPPLPASLTQLNVSYNRLIELPALPQGLKL
LNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPELLETLDVSCNGLAVLPPLPFSLQE
ISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRN
ECSIDISDNPLSSHALQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHRPLADAVTAWFPEN
KQSDVSQIWHAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQ
SFAVAADATESCEDRVALTWNNLRKTLLVHQASEGLFDNDTGALLSLGREMFRLEILEDI
ARDKVRTLHFVDEIEVYLAFQTMLAEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSR
EENEFTDWFSLWGPWHAVLKRTEADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDAD
AEREAGAQVMRETEQQIYRQLTDEVLALRLPENGSQLHHSEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-13502b
Recommended expression in:
Not Applicable