polA (B. burgdorferi B31 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  polA
Gene Name:  DNA polymerase I
Original Clone ID:
11084b1BCt8p1
Keyword:
full-length cds
Species:
Borreliella burgdorferi B31
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BcCD00534349
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1524nts
Open reading frame: 44 to 1567

AAACATAATATTTAGAAATTATCACGTAATGAAAAATTATCCACTTTTAAACACACAAGG
AGAAAATGTAAACGCATTTATTGGCTTTTTCAAAACATTATTTTTCATAATAAAAGAAAA
AAATCCTGAACATTTGATTATCACCTTTGACTCAGAGGTACCAACTTTTAGAAAACAAAA
ATATCCAAGCTACAAGGCAACAAGAGATTTACCTCCGGACGATTTAATACCTCAAATAGG
ATGGATAAAAGAAGGCCTTTTAAAGGCAAAAATACCAATCTTTGAGATGGAAGGCTACGA
AGCTGACGATCTTTTAGCTAGTTTTGCCAAAAAGGCTGCAAAGAATAACTATTTAACTTA
CATTATTTCTCCAGATAAAGACTTGCTGCAAACAATGTCAGAGTACGTAAAAATACTTAA
AATTGAAAACAACAGCTTTATTGAAATGGATAATGAGTACGTAACAAAAAAATTTGGAGT
AAATAGCTTTCAAATAAAAGATTATTTAGCTATTGTTGGAGACAGGTCTGACAATATACC
TGGAATAAAAGGCATTGGCGCAAAAGGAGCAGCAAATTTATTAAGAGAATTTAAAACCTT
AGACGGGATATATTCAAATTTAGAAATAATAAATAAAAAACACCGAGAACTTTTAATCAA
AGAAAAAGAAAATGCTTTTTTAAGCTATGAACTTGTAAGTCTTGAAGAAAATTTAAAAAT
TCCAGAAATTGAAAACTTCGCCTTAAAAAATTTTAGCGAAGAGATAATATCTTTGTTTGA
AAAGCACTCAGCAATTGCCCTAATAAAAACTATAAAAAAGGATATCTTAAAACAAGAAAA
AGAAAATGCAGACCAAAAAAGTCTATTTAAGCAAGAACCTACTACCAACAGCTTAGATGA
CATAAATACAATTGACACAGAAAATGTTAAATACCGCTCAATAACAACAAAAATAGAGCT
TGATGATTTAATAGAAAGCCTTAAAAAGGCTAAATACATATCAATAGACACAGAAACGTC
TTCGCTTGATACTTACACAGCAAAATTAATTGGGATTTCTATTTCATTTAAAGAATTTGA
AGGTTACTATATTCCAATCGAAGCCAAAGGAAAAATTTACATAGAAAAAAACTATATAAT
ACAAAAATTTAACAATCTTTTTGAATCAAATCCAAAAATAATTGGTCAAAATTATAAATT
TGACTATAAAATACTTAAAAACAATGGATTTAACCCTATACCACCTTATTTTGACACAAT
GATTGCTGCATACCTTATCGACACAAACTCAAAAGTATCGCTTGATTTTCTTGCAGAAAA
ATATTTAATGCATAAAAACATTAAATATGAAGATGTGATACAAAAAAATGACAACTTCGC
AAATATATCTTTAGAAATGGCAACAAGCTATTCATCCGAAGATGCTGATATTACATTTAG
ATTATTTAATATATTTACCAAAAAATTAAAAGAAGACAAACTCGACAAGTTAATGCACGA
AATAGAAATGCCTTTTAACAAGGTGATTATAGAAATGGAAGAAGGGCATCATCACCATCA
CCACTGATCCGAATTCGAGCTCCGTC

Protein sequence predicted based on nucleotide sequence

LLNTQGENVNAFIGFFKTLFFIIKEKNPEHLIITFDSEVPTFRKQKYPSYKATRDLPPDD
LIPQIGWIKEGLLKAKIPIFEMEGYEADDLLASFAKKAAKNNYLTYIISPDKDLLQTMSE
YVKILKIENNSFIEMDNEYVTKKFGVNSFQIKDYLAIVGDRSDNIPGIKGIGAKGAANLL
REFKTLDGIYSNLEIINKKHRELLIKEKENAFLSYELVSLEENLKIPEIENFALKNFSEE
IISLFEKHSAIALIKTIKKDILKQEKENADQKSLFKQEPTTNSLDDINTIDTENVKYRSI
TTKIELDDLIESLKKAKYISIDTETSSLDTYTAKLIGISISFKEFEGYYIPIEAKGKIYI
EKNYIIQKFNNLFESNPKIIGQNYKFDYKILKNNGFNPIPPYFDTMIAAYLIDTNSKVSL
DFLAEKYLMHKNIKYEDVIQKNDNFANISLEMATSYSSEDADITFRLFNIFTKKLKEDKL
DKLMHEIEMPFNKVIIEMEEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
ins@768,1;del@771,1
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-11084b
Recommended expression in:
Not Applicable