Gene:
Gene Symbol:
EF2447
Gene Name:
DNA internalization-related competence protein ComEC/Rec2
Original Clone ID:
GE.387.1.29_GO.10479
Keyword:
None
Species:
Enterococcus faecalis V583
Type:
cDNA
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Format:
CLOSED
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
New York Consortium on Membrane Protein Structure
EfCD00534397
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 2340nts
Open reading frame: 48 to 2387
ATCACCATCACCACCATCACGAAAACCTGTATTTTCAATCCTACGTAATGCCACACTTGA
GGACAATCCTTCCTCAGCTGAGGAATAATTGGTTCTTCATAGCTCTGCTGACTAGTTGTG
GGCTGTGGTGTTTTTTCGCACCTAGTCTATTAGGAGCTCTTCTTTTTTCTATCACTGTTA
TCCGTATTGTCTGTACAAAAAGTTTTCCTGTTATATTGGGAGGTTTTTTGTGCAGTTTTT
TAATAGTAGGGGGCATATTGATTTTCCAGTGGTTAAAGCCAGTCTCTTTGTTGCCTAAAG
AAGTCCGCTTGTCAGGACAGCTAACGATATTACCAGATGAAATACAAGTCGATGGTGATC
AAGTAAAATTTCCTGCCAATTGGATCGTAAAAGGCAAAAGGCAGAAAGTGATGGCATTTT
ATCAATTAAAAAGTGTGAAAGAAAAACAGCAATGGCAACAACTAACCACAACCATTCAGG
CAACCGCTGTAGGTATTTTAGACTCGCCCCAAGGTCAAACCAACAAAAATGGCTTCAATT
ATCAACTATTTTTAAAGAATCAAGGAATCGAAGCTATTTTATTCATTGAGGAGATAGGAC
AACCGCGTTCCTTTTTTTCTTGGCGGTTTAAAGAAATCATTAGCGGCTGGCGGAAGTGGG
CGATGGTACATATTCAGAAAAATTTTGCTGAGACTAGTGCAATGTATATGAATGCGTTGC
TATTAGGTTTTCGTAATGAAGAATTTGCTGAAATAAGCGATAAATTTTCAGCATTAGGCA
TTTTGCATCTTTTTAGTTTGTCAGGGATGCATGTTTCTTTTTTCCTTCGTTTATTTCGCT
TGCTAATGCTAAGAATTGCTCGCCGAACGCAAGAAAGTTATTTTTTGTGGCAATGCGGTT
TTTCTCTGATATATGCTGGTTTAACAGGATTCTCAGTTAGTGTCATGCGGGCGCTTTTAC
AAACAAACCTCAATACGTTCAATCGACGGTTTAATTGGCAGGTTCCGGCCCTCGATTGTT
GGAGTTTAACTCTCTTAGTGCACTTGATTGTTCAGCCAACCCTCTTTTTTTCAGTCGGCG
GTCAATTTAGTTATTTACTTTCCTTTGTTCTGCTGTTTTTAGAGCAAACATTGGTTGGTT
ATTCTTCGCCTTTCATTAAAAATTGTTTAGGCTCTTTCCTATTGGCTGTGGCTTCCTTAC
CTATTTTAGTACAATCATTTTATGAATGGCCCTTGTTAGGAAGTTTCTTAACATTTCTCT
TGTTACCAATTTTTGAACGGTTCTTGTTACCGCTTTTGACGTTGAGCTTTTTTCTAGTGA
TTATTATGCCAGCACGTTATTTAGCAGCCTTGCTCGAACCACTATTTTTAACAATGAATG
GCTTATTTGAGTGGGTGCTAGTTCACTTAAATCCGAAGCTGACCATCGGCTATTTAAATT
CTTGGATACAAATTAGTTTGTTATTACTAATCGGATTTTCAATGATTTATTTAGCAGACA
AGTCTAAAAAAATTTTTTTCTCAATCGGACTACTAATTGTTTTGATAAATAGTAAATACC
TTTCGCCAATTGGCATTGTTGCAATGATCGATGTAGGCCAAGGGGATAGTTTGTTTATCC
AAGCGCCTTTTCACAGAAAAAATACACTGATCGATACAGGGGGGCGGTTGTCTTTTGGCA
AAGAAGCTTGGCGGCAACGGAAAAATAGTCGATCAGGAGCAGAATATTCAGTGATTCCTT
TTCTCAAAAGTCAAGGAGTCAAACGTTTAGATGAAGTGATCATCACACACGCACATGAAG
ACCATTTTGGCGATTTATTAGCCATTGCGCAGAAGGTTCCAATTTCTGTTATTTATTTCC
CTAAAGGAGCAGCTGAAGCCAATTTTTCGTTTCGCCAAGTCTTGAAACAACTCCAAAAAA
GCGGGACAGACTGTCGGCCAATTTTAGCGCCCAAGCGAATTAATGGGCCCGTTTCTTTTC
AAATTTTATCACCAAATAAGAGCAGTATTGGGGGCAATAATGACTCGATAGTGTTTTCCA
CACAATTAGGCGGACGGCATTTTTTATTTACTGGTGACTTGGAAGCCGAAGGAGAAGAAC
AAGTGTTGGCTCGCTATCCCCAGTTGAAAATCGACATCTTGAAAGTCGGTCATCATGGTA
GTAAAACGTCGTCTACTAAAAGATTTATCAAACAGCTTAATCCGCGAGAAGCATTGATTT
CAGCTGGACTGAATAATCGTTTTAAACATCCTCATCAGGAGACATTAGCGACGCTGGAGG
AGCAAAAAATAAATTATTATCGGACCGATCTTCAAGGAATGATTTATTATCAATGGTTAC
CTTGGCAAGGGCTTTCTAAAGCCAAATTTGTTAAAGAACAAGACTAG
Protein sequence predicted based on nucleotide sequence
MPHLRTILPQLRNNWFFIALLTSCGLWCFFAPSLLGALLFSITVIRIVCTKSFPVILGGF LCSFLIVGGILIFQWLKPVSLLPKEVRLSGQLTILPDEIQVDGDQVKFPANWIVKGKRQK VMAFYQLKSVKEKQQWQQLTTTIQATAVGILDSPQGQTNKNGFNYQLFLKNQGIEAILFI EEIGQPRSFFSWRFKEIISGWRKWAMVHIQKNFAETSAMYMNALLLGFRNEEFAEISDKF SALGILHLFSLSGMHVSFFLRLFRLLMLRIARRTQESYFLWQCGFSLIYAGLTGFSVSVM RALLQTNLNTFNRRFNWQVPALDCWSLTLLVHLIVQPTLFFSVGGQFSYLLSFVLLFLEQ TLVGYSSPFIKNCLGSFLLAVASLPILVQSFYEWPLLGSFLTFLLLPIFERFLLPLLTLS FFLVIIMPARYLAALLEPLFLTMNGLFEWVLVHLNPKLTIGYLNSWIQISLLLLIGFSMI YLADKSKKIFFSIGLLIVLINSKYLSPIGIVAMIDVGQGDSLFIQAPFHRKNTLIDTGGR LSFGKEAWRQRKNSRSGAEYSVIPFLKSQGVKRLDEVIITHAHEDHFGDLLAIAQKVPIS VIYFPKGAAEANFSFRQVLKQLQKSGTDCRPILAPKRINGPVSFQILSPNKSSIGGNNDS IVFSTQLGGRHFLFTGDLEAEGEEQVLARYPQLKIDILKVGHHGSKTSSTKRFIKQLNPR EALISAGLNNRFKHPHQETLATLEEQKINYYRTDLQGMIYYQWLPWQGLSKAKFVKEQD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c1804a,L602I
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.10479
Recommended expression in:
Not Applicable
Vector Name:
pNYCOMPS-LIC-FH10T+ (N term)
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector; T7 promoter; N terminal Flag epitope and 10X His tags; tags can be removed by TEV protease; kanamycin resistance; ligation independent cloning.
Comments:
None
Size (bp):
6984
Parent Vector:
None
Properties:
bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name:
New York Consortium on Membrane Protein Structure
New York Structural Biology Center
New York Structural Biology Center
Publications:
PMID:
20394823
Title: High-throughput expression and purification of membrane proteins.
Vector Map:
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Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
1583
2265
death cassette
ccdB
ccdB death cassette (removed in plasmids containing an insert)
5393
5602
promoter
T7
T7 promoter
5146
5165
protease cleavage site
TEV
TEV protease cleavage site
5290
5310
repressor protein gene
LacI
Lac inhibitor
3677
4693
ribosome binding site
RBS
Ribosome Binding Site
5225
5230
selectable marker
KanR
kanamycin resistance
725
1537
selectable marker
CmR
Chloramphenicol resistance gene
6040
6696
tag
Flag
Flag epitope tag; N terminal
5236
5259
tag
His
10xHis tag; N terminal
5260
5289
trxn regulatory element
lac operator
lac operator
5165
5187
trxn termination sequence
T7 term
T7 terminator
91
137
