Gene:
Gene Symbol:
leuA
Gene Name:
2-isopropylmalate synthase
Original Clone ID:
13572a2BCt7p1
Keyword:
full-length cds
Species:
Listeria monocytogenes serotype 4b str. F2365
Type:
cDNA
Vector Name:
pSGX3
Format:
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
New York SGX Research Center for Structural Genomics
LmCD00534457
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1560nts
Open reading frame: 51 to 1610
TTCCCCAGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCCCTTAAGAAAATCC
AGTTTTTTGATACTACACTTAGAGATGGCGAACAAACCCCTGGAGTTAATTTTGACGTAA
AGGAGAAAATCCAGATTGCCTTACAACTCGAAAAACTTGGAATTGATGTAATTGAAGCCG
GCTTTCCGATTTCTTCTCCTGGAGATTTTGAATGCGTCAAGGCGATTGCAAAAGCGATTA
AACATTGTTCTGTAACAGGGCTAGCGCGCTGTGTTGAAGGAGATATTGACCGTGCCGAGG
AAGCGCTAAAAGACGCTGTTTCTCCGCAAATACATATCTTCCTTGCAACGAGCGATGTGC
ATATGGAATACAAATTAAAAATGAGTCGGGCGGAAGTTCTTGCTTCCATTAAACACCACA
TTAGTTATGCGAGACAAAAATTTGATGTTGTACAGTTTTCACCAGAAGATGCAACTCGGT
CAGATCGTGCTTTTCTGATTGAAGCAGTTCAAACAGCGATTGATGCAGGAGCAACTGTCA
TCAACATTCCAGATACGGTCGGATATACAAACCCCACCGAATTTGGGCAATTATTCCAAG
ATTTGCGCCGCGAAATAAAGCAATTTGATGATATTATTTTCGCTTCTCATTGCCATGATG
ACCTTGGTATGGCGACAGCAAACGCACTTGCTGCAATCGAAAATGGCGCAAGTCGCGTCG
AAGGTACGATTAATGGGATTGGTGAGCGTGCTGGGAATACGGCACTGGAAGAAGTTGCGG
TAGCGCTTCATATTCGCAAAGATTTTTATCAAGCTGAAACAAACATTGTATTGAATCAAT
TTAAAAATTCAAGTGATTTGATTAGCCGCTTGTCTGGTATGCCTGTTCCACGTAACAAAG
CTGTAATTGGTGGCAATGCTTACGCGCATGAATCTGGTATTCATCAAGATGGTGTCCTAA
AAAATCCTGATACGTATGAAATCATCACCCCTGCTCTTGTCGGCGTGGATAAAAACTCTC
TTCCACTTGGCAAACTTTCTGGCAAACATGCCTTCAATACGCGCATGGAAGAAATGGGCT
ACACACTAACCGAACAAGAACAAAAAGATGCCTTTAAACGTTTCAAACAATTAGCAGATG
CGAAAAAAGAAGTAACAGAAGAAGACTTGCACGCGTTAATTCTCGGACAATCTTCCGAAT
CAGCCGATGATTTCGAACTAAAACATTTGCAAGTTCAATATGTTACTGGCGGCGTCCAAG
GTGCGATTGTTCGCATTGAAGAACGTGATGGCGCTCTAGTAGAAGACGCAGCAACTGGTT
CAGGTAGCATTGAAGCGATTTATAATACGATTAACCGTCTTATGAAACAAGATATTGAAT
TAACCGATTATCGTATCCAAGCAATTACAGCCGGTCAAGATTCCCAAGCAGAAGTCCATG
TCGTCATTAAAAATGACAAAGGCGCTGTGTTCCATGGTATCGGTATTGATTTTGACGTTT
TAACTGCTAGTGCTAAAGCTTATTTGCAAGCATCAGGCAAAAGCAAAACCGCCAGTAAGC
AAGCTGATTTCGAGGAGGTAAAAGAAGGGCATCATCACCATCACCACTGATCCGAATTCG
AGCTCCGTC
Protein sequence predicted based on nucleotide sequence
KKIQFFDTTLRDGEQTPGVNFDVKEKIQIALQLEKLGIDVIEAGFPISSPGDFECVKAIA KAIKHCSVTGLARCVEGDIDRAEEALKDAVSPQIHIFLATSDVHMEYKLKMSRAEVLASI KHHISYARQKFDVVQFSPEDATRSDRAFLIEAVQTAIDAGATVINIPDTVGYTNPTEFGQ LFQDLRREIKQFDDIIFASHCHDDLGMATANALAAIENGASRVEGTINGIGERAGNTALE EVAVALHIRKDFYQAETNIVLNQFKNSSDLISRLSGMPVPRNKAVIGGNAYAHESGIHQD GVLKNPDTYEIITPALVGVDKNSLPLGKLSGKHAFNTRMEEMGYTLTEQEQKDAFKRFKQ LADAKKEVTEEDLHALILGQSSESADDFELKHLQVQYVTGGVQGAIVRIEERDGALVEDA ATGSGSIEAIYNTINRLMKQDIELTDYRIQAITAGQDSQAEVHVVIKNDKGAVFHGIGID FDVLTASAKAYLQASGKSKTASKQADFEEVKEGHHHHHH*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g51a,V17V;t99c,V33V;
a594t,R198S;g1303t,A435S
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in NYSGXRC-13572a
Recommended expression in:
Not Applicable
Vector Name:
pSGX3
Synonyms:
pSGX-3, pSGX3_BC
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial (E. coli) expression vector with T7 promoter, adds C-terminal 6xHis tag; kanamycin resistance; TOPO cloning.
Comments:
Modified from pET26b from Novagen
Size (bp):
5339
Parent Vector:
None
Properties:
TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker
Author Name:
New York SGX Research Center for Structural Genomics
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
ATG start
start
initiation start (Met, Ser, Leu)
5072
5079
TOPO recognition site
TOPO
TOPO cloning site
5076
5085
bacterial origin
ori
pBR322-type bacterial origin of replication
1421
2103
gene fragment
ROP
ROP
2527
2703
promoter
T7
T7 promoter
4983
5003
repressor protein gene
LacI
LacI CDS
3515
4531
selectable marker
KanR
kanamycin resistance gene
563
1375
tag
His
6xHis tag
5086
5103
trxn regulatory element
lac operator
lac operator
5003
5025
trxn termination sequence
T7 term
T7 terminator
5231
5307
