Gene:
Gene Symbol:
VPA0149
Gene Name:
two-component system sensor kinase
Original Clone ID:
GE.531.1.46_GO.12512
Keyword:
None
Species:
Vibrio parahaemolyticus RIMD 2210633
Type:
cDNA
Vector Name:
pNYCOMPS1
Format:
FUSION
Source:
New York Consortium on Membrane Protein Structure
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
New York Consortium on Membrane Protein Structure
New York Consortium on Membrane Protein Structure
VpCD00534467
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 1374nts
Open reading frame: 40 to 1413
AGGGGGGAGAATTCCCTCAGACTTAGAAGGAGATATACTATGATGAAGCTGGTGTGTCCA
TCCTACATAGCAAAACGCAAAGACAGCCTTGCGTTTAAGCTTTTCAGTTACATGACCGCG
ATAGTCATCAGCATTTTGCTGGTGCAAGCGTTAGCGGAACAAGCCTTGGTGAAAGCTATG
TTGAAAGTTCCAAATAGCGTCAAAATGGAAATGTTGGATCTTGCTCACCAAGCCAACGTG
CTGATTGAACATGGCGACATGGATGAGTTGGCTGATTGGGCGAACGCGCAGCAGTTCTAT
TTGTTTGTACTGAACGAGCAACTTCAACCAGTGACGCATCGTCTCATGCATCCACATTTT
GAATTTAAGCTCCAATTCAAACGCAGCATTGATGATCAATTGGAAGACAAAGTGAATCGA
CCAATCATCAGCATTCCATTGGTTCAGCAGCACAACTTAGTCATTCAACTGCCGCATCGC
TTACATCCTGCGAATGACTTCATACTGTACTTTTCCATTATCAAAGTGGTGATTGCCGCC
GTGATTTTGGCTTTGTTCTCGCTTTTAATGGCACGCAACTTACAGCAACCGTTGGACAGA
CTCAGAGAGGCGAGCAAGAAGCTTTCTAACGGCGATTTTAATGTCAGTGTTGTGCAGGAG
CTAAAGTCCAACACGCGAGAATTTAATGAACTCGCGCAAGATTTTGACCACATGACGAAA
GAGATTCACTCGCTAGCGGAGAAGCAACGTCTTTTGATTCGCGATGTGTCCCATGAACTA
AGAACGCCTCTTGCTAGACAGAACTTGGTTTTGCACCTATTGCGTAAAAGAGTTGGTGAA
AGCGAACAAGAACTAGTGACGCGGTTGGAAGACGAAGCCAAAGAGCTGAATCATCTAATC
AGTGACATTTTGGAATTTAGTCGCCTTGAGAATGCTCGCTACGACAAAGAGACCAAGCGT
AGTGATCTTGAAGTGCTACTAGCCCCACAAGTTGAGCGTTGCAAAATCGATTTGAGGGAA
TCACAGAGCATTGTATTTATTTCTCGTGTGACCGAGCCGAGAGCTTACTGTGAAGATACT
CTCGTGGTGCGTTGTCTGAATAATCTAGTTGGCAACGCTATCAAATACGCGGGTGAAGAT
GCTCGGATCGAAGTGACTCTGACTAATCAAGAAATCAATGGGCAGGCTTATCTGGTTATG
TCTGTCTCTGACGATGGGCCTGGCATCCCAGAGAACAAGTTATCCGATATCTTTAACCCG
TTTACCCGCTTGGATGCCTCGCGAGACAAAAAGCTTGGTGGATACGGCCTTGGACTGGCG
ATCGTTAAGGAATCGATGCGAATGATGGGTGGTTCCGTCAATGCAGAGAATCGTTCTCAA
GGTGGTTTAACCGTGAGTTTGGTGTTCAGACAAGCCGAAAACCTCTATTTTCAGGGACAT
CATCACCATCACCATCACCACCAT
Protein sequence predicted based on nucleotide sequence
MMKLVCPSYIAKRKDSLAFKLFSYMTAIVISILLVQALAEQALVKAMLKVPNSVKMEMLD LAHQANVLIEHGDMDELADWANAQQFYLFVLNEQLQPVTHRLMHPHFEFKLQFKRSIDDQ LEDKVNRPIISIPLVQQHNLVIQLPHRLHPANDFILYFSIIKVVIAAVILALFSLLMARN LQQPLDRLREASKKLSNGDFNVSVVQELKSNTREFNELAQDFDHMTKEIHSLAEKQRLLI RDVSHELRTPLARQNLVLHLLRKRVGESEQELVTRLEDEAKELNHLISDILEFSRLENAR YDKETKRSDLEVLLAPQVERCKIDLRESQSIVFISRVTEPRAYCEDTLVVRCLNNLVGNA IKYAGEDARIEVTLTNQEINGQAYLVMSVSDDGPGIPENKLSDIFNPFTRLDASRDKKLG GYGLGLAIVKESMRMMGGSVNAENRSQGGLTVSLVFRQ
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c138t,A46A;g282a,Q94Q;
t534a,A178A;t633c,N211N;
t780a,L260L;a783g,L261L;
c790a,R264R;t814c,L272L;
c826t,a828g,L276L;c924t,S308S;
a1005t,S335S
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in GO.12512
Recommended expression in:
Not Applicable
Vector Name:
pNYCOMPS1
Synonyms:
pNYCOMPS-1
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter, adds C-terminal EGFP and 10xHis tag; kanamycin resistance; restriction enzyme cloning (including rare-cutter cloning into MCS).
Comments:
For the expression of membrane proteins in E.Coli, producing a fusion protein with a native N-terminus, and a C-terminal fusion of EGFP and a deca histadine tag. Requires C-term IN topology of MP for EGFP to be flourescent.
Size (bp):
6040
Parent Vector:
None
Properties:
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker
Author Name:
New York Consortium on Membrane Protein Structure
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type bacterial origin of replication
1421
2103
promoter
T7
T7 promoter
4984
5003
repressor binding site
lac operator
lac operator
5003
5025
selectable marker
KanR
kanamycin resistance gene
563
1375
ssDNA origin
f1 ori
f1 origin
457
417
tag
His
C-terminal 10xHis tag
5845
5874
tag
EGFP
C-terminal EGFP tag
5131
5841
trxn regulatory element
LacI
LacI
3515
4594
trxn termination sequence
T7 term
T7 terminator
5969
6015
