prkA (Bacillus subtilis) in pSGX4

Explanation of Terms

Gene:
Gene Symbol:  prkA
Gene Name:  serine protein kinase PrkA
Original Clone ID:
10039b5BSt12p1
Keyword:
full-length cds
Species:
Bacillus subtilis
Type:
cDNA
Vector Name:
pSGX4
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
BsCD00534548
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1173nts
Open reading frame: 51 to 1223

AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTCGGCTGAAAT
GGGAAGGAACTTTCGCCGATTATTTGGAGATTATTAAAGAAAATCCGATGGTCGCGCAAT
CTGCTCATTCTCGAGTTTTTAATATGATAAAAGACAGCGGGATAGAGGAGATTGACGGAC
GCAAGAAGTACAGTTTTTTTGACCGCGAGCTGTTCGGGCTTGAAGAATCTCTGGAGCGTC
TTGTGGAAGAGTATTTTCATCCGGCTGCCAAACGTCTAGATGTCAGAAAGCGGATTCTAT
TGCTGATGGGGCCTGTGAGCGGCGGTAAATCCACGCTGGTCACAATGCTTAAAAAAGGTC
TCGAAGCGTACACGCTGACAGATAATGGCGCTGTATATGCGATTAAAGGCTGCCCGATGC
ACGAGGACCCTCTACATTTAATCCCCCATCATTTACGCGATGACTTTTACAGAGAATACG
GCATTAGAATAGAAGGAAGTCTTTCTCCTTTAAATGTCATGAGGCTGGAGGAAGAGTACG
GAGGAAGAATTGAGGATGTGAAGGTGGAGCGCATCTTCTTTTCAGAAGATAAGCGTACGG
GAATTGGAACATTCAGTCCCTCTGATCCGAAATCTCAGGATATTGCCGATTTGACGGGGA
GTATTGATTTCTCAACAATCGCCGAGTACGGTTCAGAATCAGATCCGCGCGCTTATCGAT
TTGACGGAGAGCTGAATAAAGCGAACCGCGGGATGATGGAGTTTCAGGAGATGCTGAAAT
GTGATGAGAAATTTCTCTGGCATTTGCTTTCCTTGACACAGGAAGGGAATTTTAAAGCTG
GGCGGTTTGCGTTAATTTCTGCTGATGAGCTGATCGTGGCTCACACGAATGAAACGGAGT
ATCGCTCATTTATTTCTAATAAGAAGAATGAAGCCCTGCACTCAAGAATCATCGTGATGC
CGGTTCCTTATAATTTAAAAGTGTCTGAGGAAGAGAGAATTTATGAAAAAATGATTGCAG
AAAGCGATGTGGCGGATGTTCATATTGCCCCGCATACGTTGAAGGTCGCCGCGATGTTCT
CAATCCTTACCCGGTTAAAAGAGCCGAAGCGGTCGGATATTGATCTGGTCAAGAAAATGA
GACTGTATGATGGGGAAAGTGTGGAAGGATATAACTCTGTCGACGTTGAGGATATGAAAA
AAGAATATAATGATGAAGGCTAAGGGTAACTCGAGCACCACCACCACCACCACTGAGATC
CGGCTGCTAACAAA

Protein sequence predicted based on nucleotide sequence

RLKWEGTFADYLEIIKENPMVAQSAHSRVFNMIKDSGIEEIDGRKKYSFFDRELFGLEES
LERLVEEYFHPAAKRLDVRKRILLLMGPVSGGKSTLVTMLKKGLEAYTLTDNGAVYAIKG
CPMHEDPLHLIPHHLRDDFYREYGIRIEGSLSPLNVMRLEEEYGGRIEDVKVERIFFSED
KRTGIGTFSPSDPKSQDIADLTGSIDFSTIAEYGSESDPRAYRFDGELNKANRGMMEFQE
MLKCDEKFLWHLLSLTQEGNFKAGRFALISADELIVAHTNETEYRSFISNKKNEALHSRI
IVMPVPYNLKVSEEERIYEKMIAESDVADVHIAPHTLKVAAMFSILTRLKEPKRSDIDLV
KKMRLYDGESVEGYNSVDVEDMKKEYNDEG*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-10039b
Recommended expression in:
Not Applicable