rpsA (N. meningitidis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
MR95-21.6
Keyword:
None
Species:
Neisseria meningitidis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
NpCD00536281
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1683nts
Open reading frame: 49 to 1731

CGGGCAATTCCTCTGAATATTTTGTTTACTTTAGAAGGAGATATACATATGTCTATGGAA
AATTTTGCTCACCTGTTGGAAGAAAGCTTTACCCTGCAAGAAATGAACCCGGGTGAGGTG
ATTACCGCTGAAGTAGTGGCAATCGACCAAAACTTCGTTACCGTAAACGCAGGTCTGAAA
TCAGAATCCCTGATTGATGTAGCTGAATTCAAAAACGCTCAAGGCGAAATTGAAGTTAAA
GTCGGCGACTTCGTTACCGTTACCATCGAATCCGTCGAAAACGGCTTCGGCGAAACCAAA
CTGTCCCGCGAAAAAGCCAAACGTGCAGCCGATTGGATTGCCCTGGAAGAAGCCATGGAA
AACGGCGACATCCTGTCCGGCATCATCAACGGAAAAGTCAAAGGCGGCCTGACCGTTATG
ATTAGCAGCATCCGCGCATTCCTGCCGGGTTCTTTGGTCGACGTACGTCCTGTAAAAGAC
ACTTCTCACTTCGAAGGCAAAGAGATCGAATTCAAAGTGATCAAACTGGACAAAAAACGC
AACAACGTCGTTGTTTCCCGCCGCGCCGTTCTGGAAGCCACTTTGGGTGAAGAACGCAAA
GCCCTGCTGGAAAACCTGCAAGAAGGCTCCGTCATCAAAGGCATCGTTAAAAACATTACC
GATTACGGTGCATTCGTTGACTTGGGCGGCATCGACGGTCTGTTGCACATCACCGATTTG
GCATGGCGGCGCGTGAAACACCCGAGTGAAGTCTTGGAAGTCGGTCAGGAAGTTGAAGCC
AAAGTATTGAAATTCGACCAAGAAAAACAACGCGTTTCCTTGGGTATGAAACAACTGGGC
GAAGATCCTTGGAGCGGTCTGACCCGCCGTTATCCTCAAGGCACCCGCCTGTTCGGCAAA
GTATCCAACCTGACCGACTACGGCGCATTCGTCGAAATCGAACAAGGCATCGAAGGTTTG
GTACACGTCTCCGAAATGGACTGGACCAACAAAAACGTACACCCGAGCAAAGTCGTACAA
CTGGGCGACGAAGTCGAAGTCATGATTTTGGAAATCGACGAAGGCCGCCGCCGTATCTCT
TTGGGTATGAAACAATGCCAAGCCAATCCTTGGGAAGAATTTGCCGCCAACCACAACAAA
GGCGACAAAATCTCCGGCGCGGTTAAATCCATTACCGATTTCGGCGTATTCGTCGGCCTG
CCCGGCGGCATCGACGGTTTGGTTCACCTGTCCGACCTGTCCTGGACCGAATCCGGCGAA
GAAGCCGTACGCAAATACAAAAAAGGCGAAGAAGTCGAAGCCGTCGTATTGGCAATCGAC
GTGGAAAAAGAACGCATCTCCTTGGGTATCAAACAACTGGAAGGCGATCCGTTCGGCAAC
TTCATCAGCGTGAACGACAAAGGTTCTTTGGTTAAAGGTTCCGTGAAATCTGTTGACGCC
AAAGGTGCTGTTATCGCCCTGTCTGACGAAGTAGAAGGCTACCTGCCTGCTTCCGAATTT
GCAGCCGACCGCGTTGAAGATTTGACCACCAAACTGAAAGAAGGCGACGAAGTTGAAGCC
GTCATCGTTACCGTTGACCGCAAAAACCGCAGCATCAAACTTTCCGTTAAAGCCAAAGAT
GCCAAAGAAAGCCGCGAAGCACTGAACTCCGTCAATGCCACCGCCAATGCGAATGCCGGC
ACCACCAGCTTGGGCGACCTGCTGAAAGCCAAACTCTCCGGCGAACAAGAA

Protein sequence predicted based on nucleotide sequence

MSMENFAHLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLKSESLIDVAEFKNAQGE
IEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEAMENGDILSGIINGKVKGG
LTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKLDKKRNNVVVSRRAVLEATLG
EERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGLLHITDLAWRRVKHPSEVLEVGQ
EVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYPQGTRLFGKVSNLTDYGAFVEIEQG
IEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMILEIDEGRRRISLGMKQCQANPWEEFAA
NHNKGDKISGAVKSITDFGVFVGLPGGIDGLVHLSDLSWTESGEEAVRKYKKGEEVEAVV
LAIDVEKERISLGIKQLEGDPFGNFISVNDKGSLVKGSVKSVDAKGAVIALSDEVEGYLP
ASEFAADRVEDLTTKLKEGDEVEAVIVTVDRKNRSIKLSVKAKDAKESREALNSVNATAN
ANAGTTSLGDLLKAKLSGEQE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g24c,Q8H;g1612a,A538T
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in MR95
Recommended expression in:
Not Applicable