DNASU Plasmid | PPM1L (Homo sapiens) in pSGX4

Explanation of Terms

Gene:

Gene Symbol: PPM1L

Gene Name: protein phosphatase, Mg2+/Mn2+ dependent, 1L

Original Clone ID:

8730a3BSt4p1

Keyword:

full-length cds

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pSGX4

Format:

CLOSED

Source:

New York SGX Research Center for Structural Genomics

Description:

None

Comments:

Linker not verified.

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI
New York SGX Research Center for Structural Genomics

HsCD00536721

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 924nts

Open reading frame: 51 to 974

Reference Sequence Alignment

AGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTGACGCCGTGA
AGATGGTGAAGGGCAAGGTAGCCGAGATCATGCAGAACGATCGACTCGGGGGGCTTGATG
TGCTCGAGGCCGAGTTTTCCAAGACCTGGGAGTTCAAGAACCACAACGTGGCGGTGTACT
CCATCCAGGGCCGGAGAGACCACATGGAGGACCGCTTCGAAGTTCTCACGGATCTGGCCA
ACAAGACGCACCCGTCCATCTTCGGGATCTTCGACGGGCACGGGGGAGAGACTGCAGCTG
AATATGTAAAATCTCGACTCCCAGAGGCCCTTAAACAGCATCTTCAGGACTACGAGAAAG
ACAAAGAAAATAGTGTATTATCTTACCAGACCATCCTTGAACAGCAGATTTTGTCAATTG
ACCGAGAAATGCTAGAAAAATTGACTGTATCCTATGATGAAGCAGGCACAACGTGTTTGA
TTGCTCTGCTATCAGATAAAGACCTCACTGTGGCCAACGTGGGTGACTCGCGCGGGGTCC
TGTGTGACAAAGATGGGAACGCTATTCCTTTGTCTCATGATCACAAGCCTTACCAGTTGA
AGGAAAGAAAGAGGATAAAGAGAGCAGGTGGTTTCATCAGTTTCAATGGCTCCTGGAGGG
TCCAGGGAATCCTGGCCATGTCTCGGTCCCTGGGGGATTATCCGCTGAAAAATCTCAACG
TGGTCATCCCAGACCCAGACATCCTGACCTTTGACCTGGACAAGCTTCAGCCTGAGTTCA
TGATCTTGGCATCAGATGGTCTCTGGGATGCTTTCAGCAATGAAGAAGCAGTTCGATTCA
TCAAGGAGCGCTTGGATGAACCTCACTTTGGGGCCAAGAGCATAGTTTTACAGTCATTTT
ACAGAGGCTGCCCTGACAATATAACAGTCATGGTGGTGAAGTTCAGAAATAGCAGCAAAA
CAGAAGAGCAGTAA

Protein sequence predicted based on nucleotide sequence

DAVKMVKGKVAEIMQNDRLGGLDVLEAEFSKTWEFKNHNVAVYSIQGRRDHMEDRFEVLT
DLANKTHPSIFGIFDGHGGETAAEYVKSRLPEALKQHLQDYEKDKENSVLSYQTILEQQI
LSIDREMLEKLTVSYDEAGTTCLIALLSDKDLTVANVGDSRGVLCDKDGNAIPLSHDHKP
YQLKERKRIKRAGGFISFNGSWRVQGILAMSRSLGDYPLKNLNVVIPDPDILTFDLDKLQ
PEFMILASDGLWDAFSNEEAVRFIKERLDEPHFGAKSIVLQSFYRGCPDNITVMVVKFRN
SSKTEEQ*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: kanamycin

Bacterial Selection Condition: 50ug/mL kanamycin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in NYSGXRC-8730a

Recommended expression in:

Not Applicable

Vector Name:

pSGX4

Synonyms:

pSGX-4, pSGX4_BS

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 promotor, adds N-terminal 6xHis and smt-3 tags, cleavage site; kanamycin resistance; TOPO cloning.

Comments:

Modified from pET26b from Novagen. N-terminal 6xHis-tag followed by smt3 fusion protein. Smt3 fusion cloned into NheI and BamHI sites. TOPO adaptation at BamHI and XhoI sites.

Size (bp):

5611

Parent Vector:

None

Properties:

TOPO Cloning, bacterial expression, in vitro transcription, with tag/fusion/marker

Author Name:

New York SGX Research Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

TGGCGAATGGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAATTAATTCTTAGAAAAACTCATCGAGCATCAAATGAAACTGCAATTTATTCATATCAGGATTATCAATACCATATTTTTGAAAAAGCCGTTTCTGTAATGAAGGAGAAAACTCACCGAGGCAGTTCCATAGGATGGCAAGATCCTGGTATCGGTCTGCGATTCCGACTCGTCCAACATCAATACAACCTATTAATTTCCCCTCGTCAAAAATAAGGTTATCAAGTGAGAAATCACCATGAGTGACGACTGAATCCGGTGAGAATGGCAAAAGTTTATGCATTTCTTTCCAGACTTGTTCAACAGGCCAGCCATTACGCTCGTCATCAAAATCACTCGCATCAACCAAACCGTTATTCATTCGTGATTGCGCCTGAGCGAGACGAAATACGCGATCGCTGTTAAAAGGACAATTACAAACAGGAATCGAATGCAACCGGCGCAGGAACACTGCCAGCGCATCAACAATATTTTCACCTGAATCAGGATATTCTTCTAATACCTGGAATGCTGTTTTCCCGGGGATCGCAGTGGTGAGTAACCATGCATCATCAGGAGTACGGATAAAATGCTTGATGGTCGGAAGAGGCATAAATTCCGTCAGCCAGTTTAGTCTGACCATCTCATCTGTAACATCATTGGCAACGCTACCTTTGCCATGTTTCAGAAACAACTCTGGCGCATCGGGCTTCCCATACAATCGATAGATTGTCGCACCTGATTGCCCGACATTATCGCGAGCCCATTTATACCCATATAAATCAGCATCCATGTTGGAATTTAATCGCGGCCTAGAGCAAGACGTTTCCCGTTGAATATGGCTCATAACACCCCTTGTATTACTGTTTATGTAAGCAGACAGTTTTATTGTTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGCGCCTGATGCGGTATTTTCTCCTTACGCATCTGTGCGGTATTTCACACCGCATATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATACACTCCGCTATCGCTACGTGACTGGGTCATGGCTGCGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGGCAGCTGCGGTAAAGCTCATCAGCGTGGTCGTGAAGCGATTCACAGATGTCTGCCTGTTCATCCGCGTCCAGCTCGTTGAGTTTCTCCAGAAGCGTTAATGTCTGGCTTCTGATAAAGCGGGCCATGTTAAGGGCGGTTTTTTCCTGTTTGGTCACTGATGCCTCCGTGTAAGGGGGATTTCTGTTCATGGGGGTAATGATACCGATGAAACGAGAGAGGATGCTCACGATACGGGTTACTGATGATGAACATGCCCGGTTACTGGAACGTTGTGAGGGTAAACAACTGGCGGTATGGATGCGGCGGGACCAGAGAAAAATCACTCAGGGTCAATGCCAGCGCTTCGTTAATACAGATGTAGGTGTTCCACAGGGTAGCCAGCAGCATCCTGCGATGCAGATCCGGAACATAATGGTGCAGGGCGCTGACTTCCGCGTTTCCAGACTTTACGAAACACGGAAACCGAAGACCATTCATGTTGTTGCTCAGGTCGCAGACGTTTTGCAGCAGCAGTCGCTTCACGTTCGCTCGCGTATCGGTGATTCATTCTGCTAACCAGTAAGGCAACCCCGCCAGCCTAGCCGGGTCCTCAACGACAGGAGCACGATCATGCGCACCCGTGGGGCCGCCATGCCGGCGATAATGGCCTGCTTCTCGCCGAAACGTTTGGTGGCGGGACCAGTGACGAAGGCTTGAGCGAGGGCGTGCAAGATTCCGAATACCGCAAGCGACAGGCCGATCATCGTCGCGCTCCAGCGAAAGCGGTCCTCGCCGAAAATGACCCAGAGCGCTGCCGGCACCTGTCCTACGAGTTGCATGATAAAGAAGACAGTCATAAGTGCGGCGACGATAGTCATGCCCCGCGCCCACCGGAAGGAGCTGACTGGGTTGAAGGCTCTCAAGGGCATCGGTCGAGATCCCGGTGCCTAATGAGTGAGCTAACTTACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCCAGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTAACGGCGGGATATAACATGAGCTGTCTTCGGTATCGTCGTATCCCACTACCGAGATATCCGCACCAACGCGCAGCCCGGACTCGGTAATGGCGCGCATTGCGCCCAGCGCCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTCAGCATTTGCATGGTTTGTTGAAAACCGGACATGGCACTCCAGTCGCCTTCCCGTTCCGCTATCGGCTGAATTTGATTGCGAGTGAGATATTTATGCCAGCCAGCCAGACGCAGACGCGCCGAGACAGAACTTAATGGGCCCGCTAACAGCGCGATTTGCTGGTGACCCAATGCGACCAGATGCTCCACGCCCAGTCGCGTACCGTCTTCATGGGAGAAAATAATACTGTTGATGGGTGTCTGGTCAGAGACATCAAGAAATAACGCCGGAACATTAGTGCAGGCAGCTTCCACAGCAATGGCATCCTGGTCATCCAGCGGATAGTTAATGATCAGCCCACTGACGCGTTGCGCGAGAAGATTGTGCACCGCCGCTTTACAGGCTTCGACGCCGCTTCGTTCTACCATCGACACCACCACGCTGGCACCCAGTTGATCGGCGCGAGATTTAATCGCCGCGACAATTTGCGACGGCGCGTGCAGGGCCAGACTGGAGGTGGCAACGCCAATCAGCAACGACTGTTTGCCCGCCAGTTGTTGTGCCACGCGGTTGGGAATGTAATTCAGCTCCGCCATCGCCGCTTCCACTTTTTCCCGCGTTTTCGCAGAAACGTGGCTGGCCTGGTTCACCACGCGGGAAACGGTCTGATAAGAGACACCGGCATACTCTGCGACATCGTATAACGTTACTGGTTTCACATTCACCACCCTGAATTGACTCTCTTCCGGGCGCTATCATGCCATACCGCGAAAGGTTTTGCGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTCCTGCATTAGGAAGCAGCCCAGTAGTAGGTTGAGGCCGTTGAGCACCGCCGCCGCAAGGAATGGTGCATGCAAGGAGATGGCGCCCAACAGTCCCCCGGCCACGGGGCCTGCCACCATACCCACGCCGAAACAAGCGCTCATGAGCCCGAAGTGGCGAGCCCGATCTTCCCCATCGGTGATGTCGGCGATATAGGCGCCAGCAACCGCACCTGTGGCGCCGGTGATGCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCTCGATCCCGCGAAATTAATACGACTCACTATAGGGGAATTGTGAGCGGATAACAATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACCATGGGCAGCAGCCATCATCATCATCATCACAGCAGCGGCCTGGTGCCGCGCGGCAGCCATATGGCTAGCATGTCGGACTCAGAAGTCAATCAAGAAGCTAAGCCAGAGGTCAAGCCAGAAGTCAAGCCTGAGACTCACATCAATTTAAAGGTGTCCGATGGATCTTCAGAGATCTTCTTCAAGATCAAAAAGACCACTCCTTTAAGAAGGCTGATGGAAGCGTTCGCTAAAAGACAGGGTAAGGAAATGGACTCCTTAAGATTCTTGTACGACGGTATTAGAATTCAAGCTGATCAGACCCCTGAAGATTTGGACATGGAGGATAACGATATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTAAGGGTAACTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTGCTGAAAGGAGGAACTATATCCGGAT

Vector Features:

Type

Name

Description

Start

End

TOPO recognition site

TOPO

TOPO cloning site

5435

5444

bacterial origin

ColE

ColE1 bacterial origin of replication

1421

2103

gene fragment

ROP

2527

2703

promoter

T7 promoter

4984

5003

protease cleavage site

ULP site

ULP cleavage site (recognizes the tertiary structure of SUMO)

5140

repressor protein gene

LacI

lacI cds

3515

4351

ribosome binding site

RBS

ribosome binding site (RBS)

5062

5068

selectable marker

KanR

kanamycin resistance gene

563

1375

tag

Smt3

Smt3 tag

5140

5424

tag

His

6xHis tag

5083

5100

trxn regulatory element

lac operator

5003

5025

trxn termination sequence

T7 term

T7 termination sequence

5539

5585

Gene

Gene Symbol :

PPM1L

Symbol Nomenclature :

PPM1L

SYNONYM :

PP2C-epsilon; PP2CE; PPM1-LIKE

Designation :

PP2C epsilon|Protein phosphatase 2C epsilon isoform|protein phosphatase 1L|protein phosphatase 2C isoform epsilon

Full Nomenclature :

protein phosphatase, Mg2+/Mn2+ dependent, 1L

GENEID :

151742

GI :

63003905

GenBank Accession :

NM_139245

HGNC :

16381

MIM :

611931

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00536721      ------------------------------------------------------------
NM_139245.2       ATGATAGAGGATACAATGACTTTGCTGTCTCTGCTGGGTCGCATCATGCGCTACTTCTTG
                                                                              

HsCD00536721      -------------------------------------------------AGGATAACGAT
NM_139245.2       CTGAGACCCGAGACGCTTTTCCTGCTGTGCATCAGCTTGGCTCTATGGAGTTACTTCTTC
                                                                   .  * ::* : 

HsCD00536721      ATTATTGAGGCTCACAGAGAACAGATTGGTGGATCCCTTGACGCCGTGAAGATGGTGAAG
NM_139245.2       CACACCGACGAGGTGAAGACCATCGTGAAGTCCAGCCGGGACGCCGTGAAGATGGTGAAG
                  .: *  ** *.  : *......: .* ..   .: **  *********************

HsCD00536721      GGCAAGGTAGCCGAGATCATGCAGAACGATCGACTCGGGGGGCTTGATGTGCTCGAGGCC
NM_139245.2       GGCAAGGTAGCCGAGATCATGCAGAACGATCGACTCGGGGGGCTTGATGTGCTCGAGGCC
                  ************************************************************

HsCD00536721      GAGTTTTCCAAGACCTGGGAGTTCAAGAACCACAACGTGGCGGTGTACTCCATCCAGGGC
NM_139245.2       GAGTTTTCCAAGACCTGGGAGTTCAAGAACCACAACGTGGCGGTGTACTCCATCCAGGGC
                  ************************************************************

HsCD00536721      CGGAGAGACCACATGGAGGACCGCTTCGAAGTTCTCACGGATCTGGCCAACAAGACGCAC
NM_139245.2       CGGAGAGACCACATGGAGGACCGCTTCGAAGTTCTCACGGATCTGGCCAACAAGACGCAC
                  ************************************************************

HsCD00536721      CCGTCCATCTTCGGGATCTTCGACGGGCACGGGGGAGAGACTGCAGCTGAATATGTAAAA
NM_139245.2       CCGTCCATCTTCGGGATCTTCGACGGGCACGGGGGAGAGACTGCAGCTGAATATGTAAAA
                  ************************************************************

HsCD00536721      TCTCGACTCCCAGAGGCCCTTAAACAGCATCTTCAGGACTACGAGAAAGACAAAGAAAAT
NM_139245.2       TCTCGACTCCCAGAGGCCCTTAAACAGCATCTTCAGGACTACGAGAAAGACAAAGAAAAT
                  ************************************************************

HsCD00536721      AGTGTATTATCTTACCAGACCATCCTTGAACAGCAGATTTTGTCAATTGACCGAGAAATG
NM_139245.2       AGTGTATTATCTTACCAGACCATCCTTGAACAGCAGATTTTGTCAATTGACCGAGAAATG
                  ************************************************************

HsCD00536721      CTAGAAAAATTGACTGTATCCTATGATGAAGCAGGCACAACGTGTTTGATTGCTCTGCTA
NM_139245.2       CTAGAAAAATTGACTGTATCCTATGATGAAGCAGGCACAACGTGTTTGATTGCTCTGCTA
                  ************************************************************

HsCD00536721      TCAGATAAAGACCTCACTGTGGCCAACGTGGGTGACTCGCGCGGGGTCCTGTGTGACAAA
NM_139245.2       TCAGATAAAGACCTCACTGTGGCCAACGTGGGTGACTCGCGCGGGGTCCTGTGTGACAAA
                  ************************************************************

HsCD00536721      GATGGGAACGCTATTCCTTTGTCTCATGATCACAAGCCTTACCAGTTGAAGGAAAGAAAG
NM_139245.2       GATGGGAACGCTATTCCTTTGTCTCATGATCACAAGCCTTACCAGTTGAAGGAAAGAAAG
                  ************************************************************

HsCD00536721      AGGATAAAGAGAGCAGGTGGTTTCATCAGTTTCAATGGCTCCTGGAGGGTCCAGGGAATC
NM_139245.2       AGGATAAAGAGAGCAGGTGGTTTCATCAGTTTCAATGGCTCCTGGAGGGTCCAGGGAATC
                  ************************************************************

HsCD00536721      CTGGCCATGTCTCGGTCCCTGGGGGATTATCCGCTGAAAAATCTCAACGTGGTCATCCCA
NM_139245.2       CTGGCCATGTCTCGGTCCCTGGGGGATTATCCGCTGAAAAATCTCAACGTGGTCATCCCA
                  ************************************************************

HsCD00536721      GACCCAGACATCCTGACCTTTGACCTGGACAAGCTTCAGCCTGAGTTCATGATCTTGGCA
NM_139245.2       GACCCAGACATCCTGACCTTTGACCTGGACAAGCTTCAGCCTGAGTTCATGATCTTGGCA
                  ************************************************************

HsCD00536721      TCAGATGGTCTCTGGGATGCTTTCAGCAATGAAGAAGCAGTTCGATTCATCAAGGAGCGC
NM_139245.2       TCAGATGGTCTCTGGGATGCTTTCAGCAATGAAGAAGCAGTTCGATTCATCAAGGAGCGC
                  ************************************************************

HsCD00536721      TTGGATGAACCTCACTTTGGGGCCAAGAGCATAGTTTTACAGTCATTTTACAGAGGCTGC
NM_139245.2       TTGGATGAACCTCACTTTGGGGCCAAGAGCATAGTTTTACAGTCATTTTACAGAGGCTGC
                  ************************************************************

HsCD00536721      CCTGACAATATAACAGTCATGGTGGTGAAGTTCAGAAATAGCAGCAAAACAGAAGAGCAG
NM_139245.2       CCTGACAATATAACAGTCATGGTGGTGAAGTTCAGAAATAGCAGCAAAACAGAAGAGCAG
                  ************************************************************

HsCD00536721      TAA
NM_139245.2       TGA
                  *.*