PSPPH_4247 (P. savastanoi pv. phaseolicola 1448A )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PSPPH_4247
Gene Name:  GAF domain/GGDEF domain/EAL domain-containing protein
Original Clone ID:
11201e3BCt7p1
Keyword:
None
Species:
Pseudomonas savastanoi pv. phaseolicola 1448A
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
PsCD00536999
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1845nts
Open reading frame: 51 to 1895

CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGTCCCTTAACGCCAGTG
CCCCCATTCCGCTGAATGAAACGCAACGTCTGTTGCGCATCAGGGAGCTGTGCGTACTTG
AAAACACCTCTGACGACGTGTTCGATGAAATCGTGGCGATGACCGCCGATTTCTTCCAGA
CGCCGATTGCGTTGATCTCGATTGTCGATGAACACCGTCAGTGGTTTCGCGCCAGAGTGG
GTTTGAATGCCCAGGAAACGCCACGCAACGTCTCCTTCTGTGCTTACACGATCCTTTCCG
ACTCGCTGTTTGAAATTCCGGATGCGACCCTCGACGACCGGTTCATGAACAATAGCCTGG
TGACCGGCCACCCCGATATTCGCTACTATGCGGGTGCCCCGCTGATTACCGATGACGGCA
TCGCTCTGGGCAGCCTGTGCGTGATCGACACCAAGCCGCGTGAACCGATGAGCGAGCATC
AGATGCAGATGCTCAAGCGTTTCGCCTCACTGGTCATGAAGCGCATAGTCAGCTTGCGCC
TCAGTTGCTTCATCGACCAGCCGACCGGCCTGTACAACCGGTCACGCCTGCAGGAAGACA
TCCATCAGGCACTGCTATCGAGCGTTGATTATCAGTTGGTCGTGGTGGACATGATCACCT
CTGCGTTCCTGAACGATATCGTCAAGGCTCTGGGCTACAGCTTCTCTCAGGATCTGGTGA
CAGAAATCAAGAGCCGCCTGGAGCATCTGCTGCCGCCAACCTGTTCGCTGTACAAGGTCA
GCCCTACCCGCTTCGGCTTTCTGCTGCCCGGAGAAAGAGCGCCTGATCATCTGTTTCGTA
CCATTCTCGAAGACTTCGAGACACCGGTAGAATGCCGGGGCATCCCGGTGCAGATGCAGG
TGGGTCTGGGGGTTGTCACCCTGAACCGCGACCCGACCGAAGAGCAGGACTGGATGCGCA
TGGTCATCAGCGCTGCCGATGATGCCCGCGACCGGAATCTGGGCTGGGCGATGTATGAGC
CGCACTTCGATGCCGCCCAGCAACGTGCTTTCAAACTGCTCAGTTCGTTGACCAGCGCAG
TCCACTCGCATGATCAACTGCGCCTGGTCTACCAGCCGCGCATCGACCTCGCCTCTGGCT
TGTGCACCTCGGTAGAAGCCTTGCTGCGCTGGAACCACCCGACGCTTGGTCCCATCGGAC
CTGCCGAATTCGTACCGCTGGCCGAGAAAACCGCACTCATGCGTCCGCTGAGTCTGTGGG
TGCTGACAAGCGCAATCGAGCAGGCCGCCCGCTGGCAGCAACAAGGCTTCGATTTCCGTA
TTGCGATCAACGTCACCCCGGAAGACCTTACCGGTCCGGCTTTCACTGACCGGATGATTC
GCCTGCTGGGCCAGCACAAGATCGACCCCACACGCTTTGAGCTGGAGTTCACCGAGGGCG
CGTTGATGCACAACCCGGCCGAAGTACGTCACCAGTTGGAACGCATGCGTCAGATGGGCA
TGGATGTGGCGATCGATGACTTTGGTACGGGCTACAGCAACTGGAACTACCTGCGGCAAT
TGCCCGCGACCACCGTCAAGCTCGATCAGTCGCTGATTCGCAATCTGGCGTCTGACAAGA
CCGATCAGCGTCTGGTCAAGGCACTGATCGGCCTTGCCAAGAAGCTGGGTTATTGCGTGG
TGGCCGAAGGCATCGAAACAGACGAAATTCGCCGCCTGGTCAAGCAATGGGGCTGTGATG
AGGGGCAGGGCTATCTGATTTCCAAACCTATGGAAGCCGACGCCGTACTCAACTGGCTAG
GCCCGGACCGCCGCCTGCAAACGGTCACTGAAGCCGCCGAAGCCGCTGTCATCCGCGACA
AACGGTTAGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGTCGACAAG
CT

Protein sequence predicted based on nucleotide sequence

NASAPIPLNETQRLLRIRELCVLENTSDDVFDEIVAMTADFFQTPIALISIVDEHRQWFR
ARVGLNAQETPRNVSFCAYTILSDSLFEIPDATLDDRFMNNSLVTGHPDIRYYAGAPLIT
DDGIALGSLCVIDTKPREPMSEHQMQMLKRFASLVMKRIVSLRLSCFIDQPTGLYNRSRL
QEDIHQALLSSVDYQLVVVDMITSAFLNDIVKALGYSFSQDLVTEIKSRLEHLLPPTCSL
YKVSPTRFGFLLPGERAPDHLFRTILEDFETPVECRGIPVQMQVGLGVVTLNRDPTEEQD
WMRMVISAADDARDRNLGWAMYEPHFDAAQQRAFKLLSSLTSAVHSHDQLRLVYQPRIDL
ASGLCTSVEALLRWNHPTLGPIGPAEFVPLAEKTALMRPLSLWVLTSAIEQAARWQQQGF
DFRIAINVTPEDLTGPAFTDRMIRLLGQHKIDPTRFELEFTEGALMHNPAEVRHQLERMR
QMGMDVAIDDFGTGYSNWNYLRQLPATTVKLDQSLIRNLASDKTDQRLVKALIGLAKKLG
YCVVAEGIETDEIRRLVKQWGCDEGQGYLISKPMEADAVLNWLGPDRRLQTVTEAAEAAV
IRDKRLEGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in NYSGXRC-11201e
Recommended expression in:
Not Applicable