PP_1490 (P. putida KT2440 )
In pSGX3 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  PP_1490
Gene Name:  methyltransferase, CheR-like
Original Clone ID:
11110h2BCt22p1
Keyword:
full-length cds 2-424 of 424 aa
Species:
Pseudomonas putida KT2440
Type:
cDNA
Vector Name:
pSGX3
Format: 
CLOSED
Source:
New York SGX Research Center for Structural Genomics
Description:
None
Comments:
Linker not verified.
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI
New York SGX Research Center for Structural Genomics
PdCD00537057
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1296nts
Open reading frame: 1 to 1296

AACGAACAGCGTTTCTTCCGCTTCTTGCGCGAGCGTATTGGCCTGGACGTGGAGTCGGTG
GGCGCGCCCATGGTCGAACGCGCGCTGCGCCAACGTTGCGTGGCTGCGGGCGCAATGGAC
CTGGACGATTACTGGCTGCGCCTGCAGCAATCGGCAGATGAGCAACAGGCGCTGATCGAA
GCGGTGATCGTCCCGGAAACCTGGTTTTTCCGTTACCCCGAATCCTTCACTGCGCTGGCC
AGCCTGGCCCATAAACGGCTGGCTCAACTGGCTGGCGCGCGCCCGCTGCGATTGCTCAGC
CTGCCGTGCTCTACCGGCGAGGAGCCCTATTCGCTGGCCATGGCCTTGTTTGACGCCGGC
ATGGCGCCTGGGGCATTCCTTGTCGATGGAATGGACATCAGCCCAAGTTCGGTAGCCAAG
GCCGGGCAGGCGGTATATGGGCGCAATGCGTTCCGGGGTAGCGAACTGGGCTTTCGCGAG
CGCTACTTCGATGCTCTGGACGAAGGCCACCGCCTGCATGAGCGGGTACGCCAGCAAGTC
AGCCTGCGGGTGGGCAATGTGCTTGACCCGGCGCTGGCCAGCCGCGACGGCCTGTATGAC
TTCGTGTTCTGTCGCAACCTGTTGATCTATTTCGACGTACCGACCCAACAGCGTGTGTTC
GAAGTGCTCAAGCGCTTGCTGCATCCGCAGGGTGTGCTGTTCATCGGCCCGGCCGAAGGC
AGCTTGCTGGCACGCATGGGCATGCGTCCATTGGGGATCGCCCAGTCGTTTGCCTATGTG
CGTCACGAAGGCGACAGCGCTCCCTTGGCGGCGGCACCCGCCCAAACTGCCAAGCGTGCG
TTCACGACGTTGCCCGCACCGGTTTATCCGCAGCCCAGTGTGCCGTTGCCTCGTTCGCGG
CGTGTGCTGCCGGTGGCGGCCAGGCCAGCGCGAGCCCGCGAGCACAGCCACGAGGGGGCC
AGCGAGTTGCTGGCCGGCATTGCCCGGCTGGCCAATGCCGGTGCCAGCGAGCAGGCCCGT
AGCGAATGCCAACGCTACCTGAGCCAGTACCCGCCGTCGGCACAGGTGTACTACTGGCTG
GGTTTGCTCAGTGATACCGAAGGCGATGCGCAGCAGGCGCTGAGCCACTATCGCAAGGCC
CTGTACCTGGAACCACAGCACCCCGAGGCGCTGGTGCACCTGGCCGCCTTGCTGGCGGCC
CAGGGTGACCTGGCCGGCGCTCGGCGCCTTCAGGAGCGCGCGGCGCGGGCTGGCCGGGAG
TCTGAACGAGAAGGGCATCATCACCATCACCACTGATCCGAATTCGAGCTCCGT

Protein sequence predicted based on nucleotide sequence

NEQRFFRFLRERIGLDVESVGAPMVERALRQRCVAAGAMDLDDYWLRLQQSADEQQALIE
AVIVPETWFFRYPESFTALASLAHKRLAQLAGARPLRLLSLPCSTGEEPYSLAMALFDAG
MAPGAFLVDGMDISPSSVAKAGQAVYGRNAFRGSELGFRERYFDALDEGHRLHERVRQQV
SLRVGNVLDPALASRDGLYDFVFCRNLLIYFDVPTQQRVFEVLKRLLHPQGVLFIGPAEG
SLLARMGMRPLGIAQSFAYVRHEGDSAPLAAAPAQTAKRAFTTLPAPVYPQPSVPLPRSR
RVLPVAARPARAREHSHEGASELLAGIARLANAGASEQARSECQRYLSQYPPSAQVYYWL
GLLSDTEGDAQQALSHYRKALYLEPQHPEALVHLAALLAAQGDLAGARRLQERAARAGRE
SEREGHHHHHH*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYSGXRC-11110h
Recommended expression in:
Not Applicable