rpsA (S. pneumoniae )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
SpR68-21.1
Keyword:
None
Species:
Streptococcus pneumoniae
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
SpCD00539625
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1200nts
Open reading frame: 51 to 1250

GGGGCGAATTCCCCTTAAATATTTTGTTTACTTTAGAAGGAGATATACATATGAACGAAT
TTGAAGATTTGCTAAATAGCGTTAGTCAAGTTGAGACTGGTGATGTTGTTAGTGCTGAAG
TATTGACAGTTGATGCGACTCAAGCTAACGTTGCAATCTCTGGAACTGGTGTTGAAGGTG
TCTTGACTCTTCGCGAATTGACAAACGATCGTGATGCAGATATCAATGACTTTGTTAAAG
TAGGAGAAGTATTGGATGTTCTTGTACTTCGTCAAGTAGTTGGTAAAGATACTGATACAG
TTACATACCTTGTATCTAAAAAACGCCTTGAAGCTCGCAAAGCATGGGACAAACTTGTTG
GTCGCGAAGAAGAAGTTGTTACTGTTAAAGGAACGCGTGCCGTTAAAGGTGGACTTTCAG
TAGAATTTGAAGGTGTTCGTGGATTTATCCCAGCTTCAATGTTGGATACTCGTTTCGTAC
GTAACGCTGAGCGTTTTGTAGGTCAAGAATTTGATACTAAAATCAAAGAAGTTAACGCTA
AAGAAAACCGCTTCATCCTTTCACGTCGTGAAGTTGTTGAAGCAGCTACTGCAGCAGCTC
GCGCTGAAGTATTCGGTAAATTGGCTGTTGGTGATGTTGTAACTGGTAAAGTTGCTCGTA
TCACAAGCTTCGGCGCTTTCATCGACCTTGGTGGTGTTGACGGATTGGTTCACTTGACTG
AATTGTCACATGAACGTAACGTATCACCAAAATCAGTTGTAACTGTTGGTGAAGAAATTG
AAGTGAAAATCCTTGATCTTAACGAAGAAGAAGGACGTGTATCACTTTCACTTAAAGCAA
CAGTACCAGGACCATGGGATGGCGTTGAGCAAAAATTGGCTAAAGGTGATGTAGTAGAAG
GAACAGTTAAACGTTTGACTGACTTCGGTGCATTTGTTGAAGTATTGCCAGGTATCGATG
GACTTGTTCACGTATCACAAATTTCACACAAACGGATTGAAAATCCAAAAGAAGCTCTTA
AAGTTGGTCAAGAAGTTCAAGTTAAAGTTCTTGAAGTTAACGCAGATGCAGAACGCGTGT
CACTTTCTATTAAAGCTCTTGAAGAACGTCCAGCCCAAGAAGAAGGACAAAAAGAAGAAA
AACGTGCTGCTCGTCCACGTCGTCCAAGACGTCAAGAAAAGCGTGATTTCGAACTTCCAG
AAACACAAACAGGATTTTCAATGGCTGATTTGTTTGGTGATATCGAACTT

Protein sequence predicted based on nucleotide sequence

MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGVLTLRELTNDRDADIND
FVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKLVGREEEVVTVKGTRAVKG
GLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKEVNAKENRFILSRREVVEAAT
AAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDGLVHLTELSHERNVSPKSVVTVG
EEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKLAKGDVVEGTVKRLTDFGAFVEVLP
GIDGLVHVSQISHKRIENPKEALKVGQEVQVKVLEVNADAERVSLSIKALEERPAQEEGQ
KEEKRAARPRRPRRQEKRDFELPETQTGFSMADLFGDIEL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g631a,V211I;t690c,N230N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in SpR68
Recommended expression in:
Not Applicable