rpsA (V. parahaemolyticus )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  rpsA
Gene Name:  30S ribosomal protein S1
Original Clone ID:
VpR192-21.2
Keyword:
None
Species:
Vibrio parahaemolyticus
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
VpCD00539644
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1668nts
Open reading frame: 49 to 1716

GGGCGAATTCCTCTGAATATTTTGTTAACTTTAGAAGGAGATATACATATGACTGAATCT
TTTGCTCAACTCTTTGAAGAGTTTCTAAACGAAACAGAATTCCAACAAGGCAGCATCGTT
AAAGGTACTGTAGTAGCTATCGAGAACGGTTTCGTTCTTGTTGACGCTGGTCTTAAGTCT
GAATCTGCTATCCCTGCTGAACAGTTCAAGAACGCTGCTGGCGAACTTGAAGTTGAAGTT
GGCGCTGAAGTAGACGTAGCTCTAGACGCTGTTGAAGATGGTTTCGGTGAAACTCAACTT
TCTCGTGAGAAAGCTAAGCGTCACGAAGCTTGGATCGTTCTTGAGAAAGCTTACGAAGAA
GCTGAAACTGTTGTTGGTATCATCAACGGTAAAGTTAAAGGCGGTTTCACTGTTGAACTA
AACGGTATCTGTGCTTTCCTTCCTGGCTCTCTAGTAGACGTACGTCCAATCCGCGACACT
GCTCACCTAGAAAACAAAGAGCTAGAGTTCAAAGTTATCAAACTTGACCAAAAACGTAAC
AACGTAGTTGTTTCACGTCGTGCTGTTATCGAATCTGAAAACAGCGTTGAGCGTGACGAG
CTTCTAGAAACTCTACAAGAAGGTTCTGAAGTTAAAGGTATCGTTAAGAACCTTACTGAC
TACGGTGCGTTCGTTGACCTAGGTGGCGTTGACGGTCTTCTACATATCACAGATATGGCT
TGGAAGCGCGTTAAGCACCCATCTGAAATCGTTAACGTTGGTGACGAGATCCAAGTTAAA
GTTCTTAAGTTCGATCGTGAGCGTACTCGCGTATCACTAGGTCTTAAGCAACTAGGCGAA
GATCCATGGGTAGCAATCGCTAAGCGTTACCCAGAAGGTCACAAACTAACTGGTCGCGTA
ACTAACCTAACTGACTACGGCTGCTTCGTTGAAATCGAAGAAGGCGTTGAAGGTCTAGTA
CACGTTTCTGAAATGGATTGGACTAACAAGAACATCCACCCTTCTAAAGTTGTTAATGTT
GGCGACGAAGTTGAGGTTATGGTTCTTGAAATCGACGAAGAACGTCGTCGTATCTCTCTA
GGTCTGAAACAGTGTAAAGCTAACCCATGGCAGTCATTCGCTGAAGCACAAGCTAAAGGC
GACAAAGTTACTGGTAAGATCAAGTCAATCACTGACTTCGGTATCTTCATCGGTCTAGAA
GGCGGCATCGATGGTCTAGTTCACCTATCTGACATTTCTTGGAATGTTGCTGGCGAAGAA
GCTGTACGTGAGTACAAGAAAGGCGACGAGATCTCTGCAGTTGTTCTAGCAGTAGACGCT
GAGCGTGAGCGTATCTCTCTAGGCATCAAGCAAATGGAAAACGACCCATTCAATGCTTAC
GTTGCTGACAACAAGAAAGGTACTCTAGTTAACGCTACTGTAACTGCAGTTGACGCTAAA
GGTGCTACTGTTGAAATCGCAGAAGGCGTTGAAGGTTACATCCGTGCTTCTGAAGTATCT
CGCGATCGCGTAGAAGATGCTTCTCTAATCCTAAGCGCTGGTGATGTTGTTGAAGCTAAG
TTCACAGGTGTAGACCGTAAGAACCGCGTAATCAACCTATCTATCAAAGCTAAAGATGAA
GCTGAAGAGCAAGAAGCAATGGCATCTATCAACAAGCAAGACGAAGCTGCTTTTGGTAAT
GCAATGGCTGACGCTTTCAAAGCTGCTAAAGGCGAA

Protein sequence predicted based on nucleotide sequence

MTESFAQLFEEFLNETEFQQGSIVKGTVVAIENGFVLVDAGLKSESAIPAEQFKNAAGEL
EVEVGAEVDVALDAVEDGFGETQLSREKAKRHEAWIVLEKAYEEAETVVGIINGKVKGGF
TVELNGICAFLPGSLVDVRPIRDTAHLENKELEFKVIKLDQKRNNVVVSRRAVIESENSV
ERDELLETLQEGSEVKGIVKNLTDYGAFVDLGGVDGLLHITDMAWKRVKHPSEIVNVGDE
IQVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPEGHKLTGRVTNLTDYGCFVEIEEGV
EGLVHVSEMDWTNKNIHPSKVVNVGDEVEVMVLEIDEERRRISLGLKQCKANPWQSFAEA
QAKGDKVTGKIKSITDFGIFIGLEGGIDGLVHLSDISWNVAGEEAVREYKKGDEISAVVL
AVDAERERISLGIKQMENDPFNAYVADNKKGTLVNATVTAVDAKGATVEIAEGVEGYIRA
SEVSRDRVEDASLILSAGDVVEAKFTGVDRKNRVINLSIKAKDEAEEQEAMASINKQDEA
AFGNAMADAFKAAKGE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c382t,R128C;t825a,P275P;

c1569t,D523D
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in VpR192
Recommended expression in:
Not Applicable