DNASU Plasmid | RFWD2 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RFWD2

Gene Name: ring finger and WD repeat domain 2, E3 ubiquitin protein ligase

Original Clone ID:

HR5947A-111-309-14.16

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Rutgers University
PSI
Northeast Structural Genomics Consortium

HsCD00539821

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 603nts

Open reading frame: 51 to 653

Reference Sequence Alignment

CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGCGTAAAC
GTCCGCTGCTGGCGCCGCTGTGCAACGGCCTGATTAATAGCTATGAAGATAAAAGCAACG
ATTTTGTGTGCCCGATTTGCTTTGATATGATTGAAGAAGCGTATATGACCAAATGCGGCC
ATAGCTTTTGCTATAAATGCATTCATCAGAGCCTGGAAGATAATAACCGTTGCCCGAAAT
GCAACTATGTGGTGGATAATATTGATCATCTGTATCCGAACTTTCTGGTGAACGAACTGA
TTCTGAAGCAGAAGCAGCGTTTTGAAGAAAAACGTTTTAAACTGGATCATAGCGTAAGCA
GCACCAACGGCCATCGTTGGCAGATTTTTCAGGATTGGCTGGGCACCGATCAGGATAACC
TGGATCTGGCGAACGTGAACCCGATGCTGGAACTGCTGGTGCAGAAAAAAAAGCAGCTGG
AAGCGGAAAGCCATGCGGCGCAGCTGCAGATTCTGATGGAATTTCTGAAAGTGGCGCGTC
GTAATAAACGTGAGCAGCTGGAGCAGATTCAGAAAGAACTGAGCGTGCTGGAAGAAGATA
TTAAACGTGTGGAAGAAATGAGCGGCCTGTATAGCCCGGTGAGCGAAGATTAGCTCGAGA
TCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTG

Protein sequence predicted based on nucleotide sequence

MRKRPLLAPLCNGLINSYEDKSNDFVCPICFDMIEEAYMTKCGHSFCYKCIHQSLEDNNR
CPKCNYVVDNIDHLYPNFLVNELILKQKQRFEEKRFKLDHSVSSTNGHRWQIFQDWLGTD
QDNLDLANVNPMLELLVQKKKQLEAESHAAQLQILMEFLKVARRNKREQLEQIQKELSVL
EEDIKRVEEMSGLYSPVSED*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g306a,V102V;t392c,L131P

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinExpressed: Protein_Confirmed

ProteinPurified: Protein_Purified

SolubleProtein: Protein_Soluble

Find experimental protocols in HR5947A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RFWD2

Symbol Nomenclature :

RFWD2

SYNONYM :

COP1; RNF200

Designation :

E3 ubiquitin-protein ligase RFWD2|RING finger protein 200|constitutive photomorphogenesis protein 1 homolog|putative ubiquitin ligase COP1

Full Nomenclature :

ring finger and WD repeat domain 2, E3 ubiquitin protein ligase

GENEID :

64326

Locus Tag :

RP11-318C24.3

GI :

21359963

GenBank Accession :

NM_022457

HGNC :

17440

MIM :

608067

Vega :

OTTHUMG00000034986

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCG
XM_005245447.1      ATGTCTGGTAGCCGCCAGGCCGGGTCGGGCTCCGCTGGGACAAGCCCCGGGTCCTCGGCG
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         GCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTTCCGTG
XM_005245447.1      GCCTCCTCGGTGACTTCCGCCTCCTCGTCTTTATCCTCTTCCCCGTCGCCGCCTTCCGTG
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         GCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
XM_005245447.1      GCGGTTTCGGCGGCAGCGCTGGTGTCCGGCGGGGTGGCCCAGGCCGCCGGCTCGGGCGGC
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGG
XM_005245447.1      CTCGGGGGCCCGGTGCGGCCTGTGTTGGTGGCGCCCGCCGTATCGGGTAGCGGCGGCGGG
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         GCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCGTAGGA
XM_005245447.1      GCGGTGTCCACGGGCCTGTCCCGGCACAGCTGCGCGGCCAGGCCCAGCGCCGGCGTAGGA
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         GGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
XM_005245447.1      GGCAGCAGCTCCAGCCTAGGCAGCGGCAGCAGGAAGCGACCTCTCCTCGCCCCCCTCTGC
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTT
XM_005245447.1      AACGGGCTCATCAACTCCTACGAGGACAAAAGCAACGACTTCGTATGCCCCATCTGCTTT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         GATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATT
XM_005245447.1      GATATGATTGAAGAAGCATACATGACAAAATGTGGCCACAGCTTTTGCTACAAGTGTATT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      -------------------------------------------ATGTTGTGGACAATATT
NM_022457.6         CATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
XM_005245447.1      CATCAGAGTTTGGAGGACAATAATAGATGTCCCAAGTGTAACTATGTTGTGGACAATATT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      GACCATCTGTATCCTAATTTCTTG------------------------------------
NM_022457.6         GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTT
XM_005245447.1      GACCATCTGTATCCTAATTTCTTGGTGAATGAACTCATTCTTAAACAGAAGCAAAGATTT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         GAGGAAAAGAGGTTCAAATTGGACCACTCAGTGAGTAGCACCAATGGCCACAGGTGGCAG
XM_005245447.1      GAGGAAAAGAGGTTCAAATTGGACCACTCA------------AATGGCCACAGGTGGCAG
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      ------------------------------------------------------------
NM_022457.6         ATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTT
XM_005245447.1      ATATTTCAAGATTGGTTGGGAACTGACCAAGATAACCTTGATTTGGCCAATGTCAATCTT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      -----------------------------------------GGAATCACATGCAGCCCAA
NM_022457.6         ATGTTGGAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAA
XM_005245447.1      ATGTTGGAGTTACTAGTGCAGAAGAAGAAACAACTGGAAGCAGAATCACATGCAGCCCAA
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      CTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAA
NM_022457.6         CTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAA
XM_005245447.1      CTACAGATTCTTATGGAATTCCTCAAGGTTGCAAGAAGAAATAAGAGAGAGCAACTGGAA
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      CAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGT
NM_022457.6         CAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGT
XM_005245447.1      CAGATCCAGAAGGAGCTAAGTGTTTTGGAAGAGGATATTAAGAGAGTGGAAGAAATGAGT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      GGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCA
NM_022457.6         GGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCA
XM_005245447.1      GGCTTATACTCTCCTGTCAGTGAGGATAGCACAGTGCCTCAATTTGAAGCTCCTTCTCCA
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      TCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGT
NM_022457.6         TCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGT
XM_005245447.1      TCACACAGTAGTATTATTGATTCCACAGAATACAGCCAACCTCCAGGTTTCAGTGGCAGT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      TCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTT
NM_022457.6         TCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTT
XM_005245447.1      TCTCAGACAAAGAAACAGCCTTGGTATAATAGCACGTTAGCATCAAGACGAAAACGACTT
                                                                                

HsCD00539821        ------CTTTAAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGC
NM_001286644.1      ACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCA
NM_022457.6         ACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCA
XM_005245447.1      ACTGCTCATTTTGAAGACTTGGAGCAGTGTTACTTTTCTACAAGGATGTCTCGTATCTCA
                          *:**::****.. : .:.*..**  .*:* :* * .*  ** :*:   **.* .

HsCD00539821        GTAAACGTCCGCTGCTGGCGCCGCTG------------------------TGCAACGGCC
NM_001286644.1      GATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACT
NM_022457.6         GATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACT
XM_005245447.1      GATGACAGTCGAACTGCAAGCCAGTTGGATGAATTTCAGGAATGCTTGTCCAAGTTTACT
                    *::.**.  **.:    ..***. *                          ...:  .* 

HsCD00539821        TGATTAATAGCTATGAAGATAAAAGCAACGATTTTGTGTGCCCGATTTGCTTTGATATGA
NM_001286644.1      CGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGT
NM_022457.6         CGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGT
XM_005245447.1      CGATATAATTCAGTACGACCTTTAGCCACATTGTCATATGCTAGTGATCTCTATAATGGT
                     ***::*:: *:.*..... :::***.**.:* * .*.*** .*: :*   *: *:: *:

HsCD00539821        TTGAAGAAGCGTATATGACCAAATGCGGCCATAGCTTTTGCTATAAATGCATTCATCAGA
NM_001286644.1      TCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGA
NM_022457.6         TCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGA
XM_005245447.1      TCCAGTATAGTCTCTAGTATTGAATTTGACCGGGATTGTGACTATTTTGCGATTGCTGGA
                    *  *. *:.   : ::*:. :.*:   *.*. .*.** **. :::::***.:* .  .**

HsCD00539821        GCCTGGAAGATAATAACCGTTGCCCGAAATGCAACTATGTGGTGGATAATATTGATCATC
NM_001286644.1      GTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATT
NM_022457.6         GTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATT
XM_005245447.1      GTTACAAAGAAGATTAAAGTCTATGAATATGACACTGTCATCCAGGATGCAGTGGATATT
                    *  : .****:.**:*..**  .  .*:***..***.* :   .*.::. * **.: ** 

HsCD00539821        TGTATCCGAACTTTCTGGTGAACGAACTGATTCTGAAGCAGAAGCAGCGTTTTGAAGAAA
NM_001286644.1      CATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGT
NM_022457.6         CATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGT
XM_005245447.1      CATTACCCTGAGAATGAAATGACCTGCAATTCGAAAATCAGCTGTATCAGTTGGAGTAGT
                     .*::** :.. ::  ..: .** :.*:.:*  :.** ***.:* * *. ** **. *.:

HsCD00539821        AACGTTTTAAACTG--------------------------------GATCATAGCGTAAG
NM_001286644.1      TACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGA
NM_022457.6         TACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGA
XM_005245447.1      TACCATAAGAACCTGTTAGCTAGCAGTGATTATGAAGGCACTGTTATTTTATGGGATGGA
                    :** :*::.***                                   :* **.* .*...

HsCD00539821        CAGCACCAACGGCCATCGTTGGCAGATTTTTCAGGATTGGCTGGGCACCGATCAGGATAA
NM_001286644.1      TTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGAC
NM_022457.6         TTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGAC
XM_005245447.1      TTCACAGGACAGAGGTCAAAGGTCTATCAGGAGCATGAGAAGAGGTGTTGGAGTGTTGAC
                     : ... .**.*. .**.::** . ** :  .. .: :*.. .** .  *.: :* : *.

HsCD00539821        CCTGGATCTGGCGAACGTGAACCCGATGCTGGAACTGCTGGTGCAGAAAAAAAAGCAGCT
NM_001286644.1      TTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTG
NM_022457.6         TTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTG
XM_005245447.1      TTTAATTTGATGGATCCTAAACTCTTGGCTTCAGGTTCTGATGATGCAAAAGTGAAGCTG
                      *..:*  .  **:* *.*** * : ***  *. * ***.**.:*.****.:....   

HsCD00539821        GGAAGCGGAAAGCCATGCGGCGCAGCTGCAGATTCTGATGGAATTTCTGAAAGTGGCG--
NM_001286644.1      TGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGT
NM_022457.6         TGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGT
XM_005245447.1      TGGTCTACCAATCTAGACAACTCAGTGGCAAGCATTGAGGCAAAGGCTAATGTGTGCTGT
                     *.:  . .** * * .*..* ***  ***.. : *** * **:  **.*:.   **   

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      GTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTC
NM_022457.6         GTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTC
XM_005245447.1      GTTAAATTCAGCCCCTCTTCCAGATACCATTTGGCTTTCGGCTGTGCAGATCACTGTGTC
                                                                                

HsCD00539821        ---------CGTCGTAATAAACGTGAGCAGCTGGAGCAGATTCAGAAAGAACTGAGCGTG
NM_001286644.1      CACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAA
NM_022457.6         CACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAA
XM_005245447.1      CACTACTATGATCTTCGTAACACTAAACAGCCAATCATGGTATTCAAAGGACACCGTAAA
                              .** *..***.. *.*.**** ..: .:*.*: : ****.**: .* .:.

HsCD00539821        CTGGAAGAAGATATTAAACGTGTGGAAGAAATGAGCGGCCTGTATAGCCCGGTGAGCG--
NM_001286644.1      GCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGT
NM_022457.6         GCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGT
XM_005245447.1      GCAGTCTCTTATGCAAAGTTTGTGAGTGGTGAGGAAATTGTCTCTGCCTCAACAGACAGT
                      .*:. .: **. :**.  ****..:*.:.:*....   * *.*. * *.. ...*.  

HsCD00539821        ---------------------------AAGATTAGCTCGAGATCCGGCTGCTAACAAAGC
NM_001286644.1      CAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATC
NM_022457.6         CAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATC
XM_005245447.1      CAGCTAAAACTGTGGAATGTAGGGAAACCATACTGCCTACGTTCCTTCAAGGGTCATATC
                                               ...:: :**  ..*:***  *:.  .:**:* *

HsCD00539821        CCGAAAGGAAGCTGAGTTGGCTGCTG----------------------------------
NM_001286644.1      AATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAA
NM_022457.6         AATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAA
XM_005245447.1      AATGAAAAAAACTTTGTAGGCCTGGCTTCCAATGGAGATTATATAGCTTGTGGAAGTGAA
                    .. .**..**.** :**:***                                       

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      AATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTT
NM_022457.6         AATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTT
XM_005245447.1      AATAACTCTCTCTACCTGTACTATAAAGGACTTTCTAAGACTTTGCTAACTTTTAAGTTT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      GATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTT
NM_022457.6         GATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTT
XM_005245447.1      GATACAGTCAAAAGTGTTCTCGACAAAGACCGAAAAGAAGATGATACAAATGAATTTGTT
                                                                                

HsCD00539821        ------------------------------------------------------------
NM_001286644.1      AGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAAC
NM_022457.6         AGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAAC
XM_005245447.1      AGTGCTGTGTGCTGGAGGGCACTACCAGATGGGGAGTCCAATGTGCTGATTGCTGCTAAC
                                                                                

HsCD00539821        ------------------------------------
NM_001286644.1      AGTCAGGGTACAATTAAGGTGCTAGAATTGGTATGA
NM_022457.6         AGTCAGGGTACAATTAAGGTGCTAGAATTGGTATGA
XM_005245447.1      AGTCAGGGTACAATTAAGGTGCTAGAATTGGTATGA

Pathways

NCI :

ATM pathway

NCI :

Direct p53 effectors

NCI :

p53 pathway

Panther :

P53 pathway feedback loops 1

Reactome :

Autodegradation of the E3 ubiquitin ligase COP1

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

G1/S DNA Damage Checkpoints

Reactome :

p53-Dependent G1 DNA Damage Response

Reactome :

p53-Dependent G1/S DNA damage checkpoint

Reactome :

Stabilization of p53

Protein

SMART domain :

RING : Ring finger

Structural Annotation Wiki :

HR5947A

UniProt :

Q8NHY2

HPRD :

07455

Clone

Cloning Information :

619575

HIP Master Clone ID :

516556

Original Clone ID :

HR5947A-111-309-14.16

Experimental

TargetTrack - Experimental data :

HR5947A

Organism

Species Specific ID:

64326

Genome

Chromosome :

Map Location :

1q25.1-q25.2

Ensembl :

ENSG00000143207

Reagents

Labome :

COP1-antibody

RFWD2 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

RFWD2 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)