dnaA (H. halodurans )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  dnaA
Gene Name:  chromosome replication initiator DnaA
Original Clone ID:
BhR154-21.1
Keyword:
None
Species:
Halalkalibacterium halodurans
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
BhCD00540537
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1347nts
Open reading frame: 51 to 1397

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGGAAAATA
TCCATGATTTATGGGAGCGCGCCTTGAAATCGATGGAAAAAAAGGTGAGCAAACCGAGCT
TCGAGACATGGTTAAAGCAAACAAAGGCAAACAGCATTGAAGATAGCACGATCATCATAA
CAGCACCAAATGAATTTGCTCGGGACTGGTTGGAGAAACATTACGACGAGCTCATTTCTG
AAACAATTGATGATCTAACAGGTGTTCGCCTATATCCAAAGTTCGTCATTCCGACAAGTC
AACTTGATGAACCTTTCGTTGAGCAAGAATTGAAAAAGCCGATGAAACAGCCTCCAGCAC
AAAATGGAGAAATGCCTAATAACATGCTCAATGACAAATACACATTCGATACGTTTGTCA
TCGGATCTGGCAACCGCTTCGCTCACGCAGCATCACTTGCGGTAGCGGAAGCTCCTGCAA
AAGCATACAACCCACTTTTTATTTATGGTGGAGTCGGTCTGGGAAAAACCCACTTAATGC
ACGCCATCGGACATTATGTGATGGATCATAATCCGAATGCGAAAGTCGTCTATTTGTCAT
CTGAGAAATTTACGAATGAATTTATTAATGCGATTCGTGACAACAAAGCGGTCAATTTCC
GAAACAAATATCGAAATGTTGATGTGTTGCTGATTGACGATATTCAGTTTCTCGCAGGAA
AAGAGCAAACACAGGAAGAGTTCTTCCATACGTTTAATGCACTTCATGAAGATAACAAGC
AGATTGTCATCTCAAGCGATCGCCCTCCTAAGGAAATTCCAACGTTGGAAGATCGCTTGC
GCTCTCGATTTGAGTGGGGCTTAATTACTGACATTACTCCACCAGATTTAGAAACGAGAA
TTGCGATTTTACGGAAAAAGGCGAAGGCTGAAAACCTCGACATCCCAAATGAAGTGATGC
TCTATATTGCCAATCAAATTGATACGAACATTCGTGAACTAGAGGGAGCGCTCATCCGCG
TCGTCGCTTATTCATCCCTCATTAATCAGGATATGAATGCAGATCTAGCAGCAGAAGCAT
TGAAAGATATTATCCCAAATGCTAAGCCGCGCGTGTTGACAATTACCGATATTCAAAAAA
CCGTTGGCGAGTATTTCCATGTGAAACTTGAAGACTTTAAAGCGAAAAAACGAACAAAAT
CCGTTGCTTTCCCAAGGCAAATTGCCATGTATCTATCGAGGGAGCTAACGGATGCCTCCC
TGCCGAAAATCGGAAGCGAATTTGGAGGCCGGGATCATACGACTGTCATTCATGCCCATG
AAAAAATTTCAAAGCTTCTTTCAACAGACCAAGAATTACAGGATAAAATCCAAGATATTA
GCGACAAGCTACGCTCA

Protein sequence predicted based on nucleotide sequence

MENIHDLWERALKSMEKKVSKPSFETWLKQTKANSIEDSTIIITAPNEFARDWLEKHYDE
LISETIDDLTGVRLYPKFVIPTSQLDEPFVEQELKKPMKQPPAQNGEMPNNMLNDKYTFD
TFVIGSGNRFAHAASLAVAEAPAKAYNPLFIYGGVGLGKTHLMHAIGHYVMDHNPNAKVV
YLSSEKFTNEFINAIRDNKAVNFRNKYRNVDVLLIDDIQFLAGKEQTQEEFFHTFNALHE
DNKQIVISSDRPPKEIPTLEDRLRSRFEWGLITDITPPDLETRIAILRKKAKAENLDIPN
EVMLYIANQIDTNIRELEGALIRVVAYSSLINQDMNADLAAEALKDIIPNAKPRVLTITD
IQKTVGEYFHVKLEDFKAKKRTKSVAFPRQIAMYLSRELTDASLPKIGSEFGGRDHTTVI
HAHEKISKLLSTDQELQDKIQDISDKLRS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
Find experimental protocols in BhR154
Recommended expression in:
Not Applicable