SO_4017 (S. oneidensis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  mltD
Gene Name:  membrane bound lytic murein transglycosylase MltD
Original Clone ID:
SoR123-21.1
Keyword:
None
Species:
Shewanella oneidensis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
SoCD00540828
Price: 
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1431nts
Open reading frame: 51 to 1481

GGGAGGACATTCCTCTAGATATTTTGTTTACTTTAGAAGGAGATATACATATGCGGAAAA
CCTTTCGTCAACCCCATGCATTAGCTGCGATGGTTATTGCCATGCTGATGGCCCAGCTAA
GTGGCTGCGCAACAACCTCGGCACAGACATCGCCAACGGAATTTGGTGCTGGTTTACCAG
AAGCCGAAGATCATAGCGAATCAGCAGAACCTCAATCAGTTGAAGCTGAGGTTATCGACG
AGCGTTATGCCGATGTGTGGGAGAAAATCCAACACGCCCGCACTATTGATGTACATGACG
ATGCAGAAGTACGCAAACAACATAACTTTTTCGATGATAAACAAAAATTTATGACTCAGG
TGACACAGCGCGCCGAGCCATTTTTATATTATATTGTCAGCCAACTTGAGGCTCGCAATA
TGCCGCTTGAACTGGCACTCCTGCCAATTGTAGAGAGTGGTTACAATCCGCTCGCTCAGG
CAAATGGCCCCGCCGGACTTTGGCAAATGATCCCCGCCACGGGGCGTAACTTTGGCCTAA
CCATCAACTCAGCCTACGATGGCCGCAAAGATGCTCTGGCCTCAACCGATGCGGTACTCG
ACTATCTCCAACATTTATACAATACCCTAGGCAATGACTGGATTAACGCCGTAGCGGGTT
ACAACAGTGGCGAACTGGTCATCAAAGCTGCCATTGATAGAAATAAAGCCAAGGGCAAAC
CCACGGATTTTTGGTCGCTCAATATTCCGGCTCGCCAAGTGCAAACCGTACCTAAATGGC
TAGCGTTTATCCAAATTATCCGCGAACCCAGCCATTACAATCTTAAAGTGATGCCGATTG
CAAACCGTCCCTTTTTAGAACGCTTACCCGCTCCCAATGGCGTTGAGATAAACCAAATCG
CCAATGCTGCGGGACTCACTAAAGCGGAGTTCAAAACCTATAACCCAGGATACCGCCAAA
GCGTGATCCCCAGTAAAGGCAAATACCAAATTGCCCTGCCGATTGAAAACATTGGCCACT
ATCAAGAAAACCAGCACAAGCTATATGCACAAAAACGTTACGACAGCCAAACCTATATTG
TGAAATCAGGTGATAGCTTAGGCACTATTGCGGCTAAATTTGATTTATCAGTCAAAGAGT
TAAAACAGGCGAATAATCTGACCTCAGATCGCCTTAAAATCGGCCAAGAGCTTACACTTT
TAACCCCAATGGCGGCCAATGATAATGAGCCAGAACCAGTAAAGACCAAGGCTAATACCG
ATAAATCATCGACCAAAGCGAATGCGAGTAGCACTAAAGCTACCAGCTCAAAATCGACCG
ATAAGCCAGTGGCAAAAGCCAAAACCTACAAAGTAAAATCAGGGGATTCACTAGATAAAA
TCGCGCGTAAAAGCAAAGTGAAGCTCGCCGATTTAATGAAGTGGAACCAGCTAAATGCCA
AGAGCATCATCAAGCCAGGTCAAGAACTCAAAATAAGCGAA

Protein sequence predicted based on nucleotide sequence

MRKTFRQPHALAAMVIAMLMAQLSGCATTSAQTSPTEFGAGLPEAEDHSESAEPQSVEAE
VIDERYADVWEKIQHARTIDVHDDAEVRKQHNFFDDKQKFMTQVTQRAEPFLYYIVSQLE
ARNMPLELALLPIVESGYNPLAQANGPAGLWQMIPATGRNFGLTINSAYDGRKDALASTD
AVLDYLQHLYNTLGNDWINAVAGYNSGELVIKAAIDRNKAKGKPTDFWSLNIPARQVQTV
PKWLAFIQIIREPSHYNLKVMPIANRPFLERLPAPNGVEINQIANAAGLTKAEFKTYNPG
YRQSVIPSKGKYQIALPIENIGHYQENQHKLYAQKRYDSQTYIVKSGDSLGTIAAKFDLS
VKELKQANNLTSDRLKIGQELTLLTPMAANDNEPEPVKTKANTDKSSTKANASSTKATSS
KSTDKPVAKAKTYKVKSGDSLDKIARKSKVKLADLMKWNQLNAKSIIKPGQELKISE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g272a,R91H;g842a,S281N
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
SolubleProtein: Protein_Soluble
ProteinPurified: Tested_Not_Purified
Find experimental protocols in SoR123
Recommended expression in:
Not Applicable