CPE1231 (C. perfringens )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CPE1231
Gene Name:  surface protein
Original Clone ID:
CpR82G-548-608-21.12
Keyword:
None
Species:
Clostridium perfringens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
CpCD00540864
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 186nts
Open reading frame: 51 to 236

AATTCCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGACTGAAA
AAACAGGAGTAGTAAATGTAAGTAGCTCTTTAAACGTAAGAGAAGGAGCAAGTACAAGCA
GTAAAGTTATAGGAAGCTTAAGCGGAAACACAAAGGTAACAATAGTAGGAGAAGAAGGAG
CCTTCTATAAAATAGAATATAAAGGTTCTCATGGGTATGTAGCTAAGAAATACATTCTCG
AGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MTEKTGVVNVSSSLNVREGASTSSKVIGSLSGNTKVTIVGEEGAFYKIEYKGSHGYVAKK
YI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c33t,S11S;t36c,S12S;

c40t,L14L;t69c,S23S;

t110c,I37T;a132c,A44A;

c138t,Y46Y;g153a,K51K;

g178a,E60K
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in CpR82G
Recommended expression in:
Not Applicable