Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
pGTDelta238
Keyword:
None
Species:
Tobacco etch virus
Type:
cDNA
Vector Name:
pMinVecK
Format:
FUSION
Source:
Center for Eukaryotic Structural Genomics
Description:
None
Comments:
This vector only exists in silico
Mutation / Discrepancy:
No / No
Publications:
PMID: 17543538
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
Authors:
University of Wisconsin Madison
Paul Blommel
Brian Fox
PSI
Center for Eukaryotic Structural Genomics
Paul Blommel
Brian Fox
PSI
Center for Eukaryotic Structural Genomics
TvCD00541083
Price:
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 651nts
Open reading frame: 1 to 651
ATGGGTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTG
GAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGG
CGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGT
GATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATG
TTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGAT
ATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGAT
TTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACA
TATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTT
GTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAA
CGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGG
CCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA
Protein sequence predicted based on nucleotide sequence
MGILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDG DVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVD FLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKK RIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
ProteinExpressed: Not_Tested
Recommended expression in:
Not Applicable
Vector Name:
pMinVecK
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector used in the pGTDelta238 plasmid with a T5 promoter; kanamycin resistance in bacteria; restriction enzyme cloning
Comments:
This vector only exists in silico
Size (bp):
3914
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site
Author Name:
University of Wisconsin
Brian Fox
Paul Blommel
Center for Eukaryotic Structural Genomics
Brian Fox
Paul Blommel
Center for Eukaryotic Structural Genomics
Publications:
PMID:
17543538
Title: A combined approach to improving large-scale production of tobacco etch virus protease.
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
pBR322 origin
pBR322 origin
1711
2938
primer
forward primer
"Upstream NcoI" forward sequencing primer 5' TTGCTTTGTGAGCGGATAAC 3'
141
160
primer
reverse primer
"MinVec Rev" reverse sequencing primer 5' AAGCTTCGAACGCGTATCG 3'
310
329
promoter
T5
T5 promoter
122
202
repressor protein gene
LacI
LacI-wt coding region
541
1632
selectable marker
KanR
Kanamycin resistance gene
2946
3740
trxn termination sequence
rrnB
rrnB terminator
357
453
