J6R (Vaccinia virus WR) in pDONR222 (Gateway donor/master)

Explanation of Terms

Gene:
Gene Symbol:  J6R
Gene Name:  temporal expression: early/late
Original Clone ID:
VV098-8
Keyword:
RNA pol; 2 or more aa changes
Species:
Vaccinia virus WR
Type:
cDNA
Vector Name:
pDONR222
Format: 
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Nancy Villa
VvCD00543601
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 3858nts
Open reading frame: 1 to 3858

ATGGCTGTAATCTCTAAGGTTACGTATAGTCTATATGATCAAAAAGAGATTAATGCTACA
GATATTATCATTAGTCATGTTAAAAATGACGACGATATCGGTACCGTTAAAGATGGTAGA
CTAGGTGCTATGGATGGGGCATTATGTAAGACTTGTGGGAAAACGGAATTGGAATGTTTC
GGTCACTGGGGTAAAGTAAGTATTTATAAAACTCATATAGTTAAGCCTGAATTTATTTCA
GAAATTATTCGTTTACTGAATCATATATGTATTCACTGCGGATTATTGCGTTCACGAGAA
CCGTATTCCGACGATATTAACCTAAAAGAGTTATCGGGACACGCTCTTAGGAGATTAAAG
GATAAAATATTATCCAAGAAAAAGTCATGTTGGAACAGTGAATGTATGCAACCGTATCAA
AAAATTACTTTTTCAAAGAAAAAGGTTTGTTTCGTCAACAAGTTGGATGATATTAACGTT
CCTAATTCTCTCATCTATCAAAAGTTAATTTCTATTCATGAAAAGTTTTGGCCATTATTA
GAAATTCATCAATATCCAGCTAACTTATTTTATACAGACTACTTTCCCATCCCTCCGTTG
ATTATTAGACCGGCTATTAGTTTTTGGATAGATAGTATACCCAAAGAAACCAATGAATTA
ACTTACTTATTAGGTATGATCGTTAAGAATTGTAACTTGAATGCTGATGAACAGGTTATC
CAGAAGGCGGTAATAGAATACGATGATATTAAAATTATTTCTAATAACACTTCCAGTATC
AATTTATCATATATTACATCCGGCAAAAATAATATGATTAGAAGTTATATCGTCGCCCGA
CGAAAAGATCAGACGGCTAGATCTGTAATTGGTCCCAGTACATCTATCACCGTTAATGAG
GTAGGAATGCCCGCATATATTAGAAATACACTTACAGAAAAGATATTTGTTAATGCCTTT
ACAGTGGATAAAGTTAAACAACTATTAGCATCAAACCAAGTTAAATTTTACTTTAATAAA
CGATTAAACCAATTAACAAGAATACGCCAAGGAAAGTTTATCAAAAATAAAATACATTTA
TTGCCTGGTGATTGGGTAGAAGTAGCTGTTCAAGAATATACAAGTATTATTTTTGGAAGA
CAGCCGTCTCTACATAGATACAACGTCATCGCTTCATCTATCAGAGCTACCGAAGGAGAT
ACTATCAAAATATCTCCCGGAATTGCCAACTCTCAAAATGCTGATTTCGACGGAGATGAA
GAATGGATGATATTAGAACAAAATCCTAAAGCTGTAATTGAACAAAGTATTCTTATGTAT
CCGACGACGTTACTCAAACACGATATTCATGGAGCCCCCGTTTATGGATCTATTCAAGAT
GAAATCGTAGCAGCGTATTCATTGTTTAGGATACAAGATCTTTGTTTAGATGAAGTATTG
AACATCTTGGGGAAATATGGAAGAGAGTTCGATCCTAAAGGTAAATGTAAATTCAGCGGT
AAAGATATCTATACTTACTTGATAGGTGAAAAGATTAATTATCCGGGTCTCTTAAAGGAT
GGTGAAATTATTGCAAACGACGTAGATAGTAATTTTGTTGTGGCTATGAGGCATCTGTCA
TTGGCTGGACTCTTATCCGATCATAAGTCGAACGTGGAAGGTATCAACTTTATTATCAAG
TCATCTTATGTTTTTAAGAGATATCTATCTATTTACGGTTTTGGGGTGACATTCAAAGAT
CTGAGACCAAATTCGACGTTCACTAATAAATTGGAGGCCATCAACGTAGAAAAAATAGAA
CTTATCAAAGAAGCATACGCCAAATATCTCAACGATGTAAGAGACGGGAAAATAGTTCCA
TTATCTAAAGCTTTAGAGGCGGACTATGTGGAATCCATGTTATCCAACTTGACAAATCTT
AATATCCGAGAGATAGAAGAACATATGAGACAAACGCTGATAGATGATCCAGATAATAAC
CTCCTGAAAATGGCCAAAGCGGGTTATAAAGTAAATCCTACAGAACTAATGTATATTCTA
GGTACGTATGGACAACAAAGGATTGATGGTGAACCAGCAGAGACTCGAGTATTGGGTAGA
GTCTTACCTTACTATCTTCCAGACTCTAAGGATCCAGAAGGAAGAGGTTACATTCTTAAT
TCTTTAACAAAAGGATTAACAGGTTCTCAATATTACTTTTCGATGCTGGTTGCAAGATCT
CAATCTACTGATATCGTCTGTGAAACATCACGTACCGGAACACTGGCTAGAAAAATCATT
AAAAAGATGGAGGATATGGTGGTCGACGGATACGGACAAGTAGTTATAGGTAATACGCTC
ATCAAGTACGCCGCCAATTATACCAAAATTCTAGGCTCAGTATGTAAACCTGTAGATCTT
ATCTATCCAGATGAGTCCATGACTTGGTATTTGGAAATTAGTGCTCTGTGGAATAAAATA
AAACAGGGATTCGTTTACTCTCAGAAACAGAAACTTGCAAAGAAGACATTGGCGCCGTTT
AATTTCCTAGTATTCGTCAAACCCACCACTGAGGATAATGCTATTAAGGTTAAGGATCTG
TACGATATGATTCATAACGTCATTGATGATGTGAGAGAGAAATACTTCTTTACGGTATCT
AATATAGATTTTATGGAGTATATATTCTTGACGCATCTTAATCCTTCTAGAATTAGAATT
ACAAAAGAAACGGCTATCACTATCTTTGAAAAGTTCTATGAAAAACTCAATTATACTCTA
GGTGGTGGAACTCCTATTGGAATTATTTCTGCACAGGTATTGTCTGAGAAGTTTACACAA
CAAGCCCTGTCCAGTTTTCACACTACTGAAAAAAGTGGTGCCGTCAAACAAAAACTTGGT
TTCAACGAGTTTAATAACTTGACTAATTTGAGTAAGAATAAGACCGAAATTATCACTCTG
GTATCCGATGATATCTCTAAACTTCAATCTGTTAAGATTAATTTCGAATTTGTATGTTTG
GGAGAATTAAATCCAGACATCACTCTTCGAAAAGAAACAGATAGGTATGTAGTAGATATA
ATAGTCAATAGATTATACATCAAGAGAGCAGAAATTACCGAATTAGTCGTCGAATATATG
ATTGAACGATTCATCTCCTTTAGCGTCATTGTAAAGGAATGGGGTATGGAAACATTCATT
GAGGACGAGGATAATATTAGATTTACTGTCTATCTAAATTTCGTTGAACCAGAGGAATTG
AATCTTAGTAAGTTTATGATGGTTCTTCCGGGGGCAGCCAACAAGGGAAAGATTAGTAAA
TTCAAGATTCCTATCTCTGATTATACGGGTTATGACGACTTCAATCAAACAAAAAAGCTC
AATAAGATGACTGTAGAACTCATGAATCTAAAAGAATTAGGTTCTTTCGATTTGGAAAAC
GTCAACGTGTATCCTGGAGTATGGAATACATACGATATCTTCGGTATCGAGGCCGCTCGT
GAATACTTGTGCGAAGCCATGTTAAACACCTATGGAGAAGGGTTCGATTATCTGTATCAG
CCTTGTGATCTTCTCGCTAGTTTACTATGTGCTAGTTACGAACCAGAATCAGTGAATAAA
TTCAAGTTCGGCGCAGCTAGTACTCTTAAGAGAGCTACGTTCGGAGACAATAAAGCATTG
TTAAACGCGGCTCTTCATAAAAAGTCAGAACCTATTAACGATAATAGTAGCTGCCACTTT
TTTAGCAAGGTCCCTAATATAGGAACTGGATATTACAAATACTTTATCGACTTGGGTCTT
CTCATGAGAATGGAAAGGAAACTATCTGATAAGATATCTTCTCAAAAGATCAAGGAAATG
GAAGAAACAGAAGACTTT

Protein sequence predicted based on nucleotide sequence

MAVISKVTYSLYDQKEINATDIIISHVKNDDDIGTVKDGRLGAMDGALCKTCGKTELECF
GHWGKVSIYKTHIVKPEFISEIIRLLNHICIHCGLLRSREPYSDDINLKELSGHALRRLK
DKILSKKKSCWNSECMQPYQKITFSKKKVCFVNKLDDINVPNSLIYQKLISIHEKFWPLL
EIHQYPANLFYTDYFPIPPLIIRPAISFWIDSIPKETNELTYLLGMIVKNCNLNADEQVI
QKAVIEYDDIKIISNNTSSINLSYITSGKNNMIRSYIVARRKDQTARSVIGPSTSITVNE
VGMPAYIRNTLTEKIFVNAFTVDKVKQLLASNQVKFYFNKRLNQLTRIRQGKFIKNKIHL
LPGDWVEVAVQEYTSIIFGRQPSLHRYNVIASSIRATEGDTIKISPGIANSQNADFDGDE
EWMILEQNPKAVIEQSILMYPTTLLKHDIHGAPVYGSIQDEIVAAYSLFRIQDLCLDEVL
NILGKYGREFDPKGKCKFSGKDIYTYLIGEKINYPGLLKDGEIIANDVDSNFVVAMRHLS
LAGLLSDHKSNVEGINFIIKSSYVFKRYLSIYGFGVTFKDLRPNSTFTNKLEAINVEKIE
LIKEAYAKYLNDVRDGKIVPLSKALEADYVESMLSNLTNLNIREIEEHMRQTLIDDPDNN
LLKMAKAGYKVNPTELMYILGTYGQQRIDGEPAETRVLGRVLPYYLPDSKDPEGRGYILN
SLTKGLTGSQYYFSMLVARSQSTDIVCETSRTGTLARKIIKKMEDMVVDGYGQVVIGNTL
IKYAANYTKILGSVCKPVDLIYPDESMTWYLEISALWNKIKQGFVYSQKQKLAKKTLAPF
NFLVFVKPTTEDNAIKVKDLYDMIHNVIDDVREKYFFTVSNIDFMEYIFLTHLNPSRIRI
TKETAITIFEKFYEKLNYTLGGGTPIGIISAQVLSEKFTQQALSSFHTTEKSGAVKQKLG
FNEFNNLTNLSKNKTEIITLVSDDISKLQSVKINFEFVCLGELNPDITLRKETDRYVVDI
IVNRLYIKRAEITELVVEYMIERFISFSVIVKEWGMETFIEDEDNIRFTVYLNFVEPEEL
NLSKFMMVLPGAANKGKISKFKIPISDYTGYDDFNQTKKLNKMTVELMNLKELGSFDLEN
VNVYPGVWNTYDIFGIEAAREYLCEAMLNTYGEGFDYLYQPCDLLASLLCASYEPESVNK
FKFGAASTLKRATFGDNKALLNAALHKKSEPINDNSSCHFFSKVPNIGTGYYKYFIDLGL
LMRMERKLSDKISSQKIKEMEETEDF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable