K4L (Vaccinia virus WR) in pDONR222 (Gateway donor/master)

Explanation of Terms

Gene:
Gene Symbol:  K4L
Gene Name:  phospholipase-D-like protein
Original Clone ID:
VV035
Keyword:
Nicking-Joining Enzyme (Cop-K4L)
Species:
Vaccinia virus WR
Type:
cDNA
Vector Name:
pDONR222
Format: 
FUSION
Source:
University of Utah
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
University of Utah
Leiliang Zhang
VvCD00543613
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 1272nts
Open reading frame: 1 to 1272

ATGAATCCGGATAATACAATCGCAGTGATTACAGAGACTATTCCTATAGGTATGCAATTT
GATAAAGTATATTTGTCTACATTTAACATGTGGAGGGAAATTCTATCCAATACCACAAAA
ACACTAGATATATCATCTTTTTATTGGAGTTTATCGGATGAAGTGGGTACGAATTTCGGC
ACGATAATATTAAACAAGATTGTACAATTACCCAAAAGAGGAGTACGAGTTAGAGTAGCC
GTCAATAAATCTAACAAACCATTAAAGGATGTTGAAAGACTACAAATGGCCGGAGTTGAA
GTACGATACATAGATATTACAAATATCCTAGGAGGAGTTCTTCATACAAAATTTTGGATA
TCTGATAATACACATATTTATTTAGGAAGCGCTAACATGGATTGGAGATCACTAACTCAG
GTCAAAGAATTGGGTATTGCGATCTTCAATAATAGGAACTTGGCAGCGGATCTCACTCAA
ATTTTTGAGGTATACTGGTATCTTGGAGTTAACAATCTACCATATAATTGGAAAAACTTT
TATCCGTCGTATTATAATACAGATCATCCTCTTAGTATTAACGTAAGCGGCGTTCCACAC
TCTGTATTTATTGCTTCTGCACCGCAACAACTATGTACTATGGAAAGAACCAATGATTTA
ACCGCTTTATTGTCATGTATCAGAAATGCGAGTAAATTCGTTTATGTATCTGTTATGAAC
TTTATCCCTATTATTTATTCGAAGGCAGGCAATATTTTGTTTTGGCCTTATATAGAAGAT
GAATTAAGAAGAGCCGCTATAGACAGACAAGTGTCAGTTAAGCTATTGATTAGTTGCTGG
CAACGATCTTCGTTTATCATGAGAAACTTTTTAAGATCTATCGCTATGCTAAAATCTAAA
AACATAAATATAGAAGTAAAGCTATTTATTGTACCAGATGCTGATCCTCCCATTCCGTAT
TCTAGGGTAAACCATGCCAAATATATGGTAACCGATAAAACGGCGTATATAGGTACCTCA
AATTGGACAGGAAATTACTTTACGGATACATGTGGAGCATCTATTAATATTACACCGGAT
GATGGATTAGGTCTTCGTCAACAATTGGAAGATATTTTTATGCGTGATTGGAATTCAAAA
TACAGCTATGAATTGTACGATACTAGTCCTACTAAAAGGTGTAGACTATTAAAAAATATG
AAACAATGTACAAATGATATATACTGCGATGAGATACAACCGGAAAAAGAAATTCCTGAA
TATTCTCTTGAA

Protein sequence predicted based on nucleotide sequence

MNPDNTIAVITETIPIGMQFDKVYLSTFNMWREILSNTTKTLDISSFYWSLSDEVGTNFG
TIILNKIVQLPKRGVRVRVAVNKSNKPLKDVERLQMAGVEVRYIDITNILGGVLHTKFWI
SDNTHIYLGSANMDWRSLTQVKELGIAIFNNRNLAADLTQIFEVYWYLGVNNLPYNWKNF
YPSYYNTDHPLSINVSGVPHSVFIASAPQQLCTMERTNDLTALLSCIRNASKFVYVSVMN
FIPIIYSKAGNILFWPYIEDELRRAAIDRQVSVKLLISCWQRSSFIMRNFLRSIAMLKSK
NINIEVKLFIVPDADPPIPYSRVNHAKYMVTDKTAYIGTSNWTGNYFTDTCGASINITPD
DGLGLRQQLEDIFMRDWNSKYSYELYDTSPTKRCRLLKNMKQCTNDIYCDEIQPEKEIPE
YSLE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
None
Recommended expression in:
Not Applicable