SMa0034 (S. meliloti 1021 )
In pNIC28-Bsa4 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  SMa0034
Gene Name:  protease
Original Clone ID:
20543
Keyword:
None
Species:
Sinorhizobium meliloti 1021
Type:
cDNA
Vector Name:
pNIC28-Bsa4
Format: 
CLOSED
Source:
New York Structural Genomics Research Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Albert Einstein College of Medicine
Steven Almo
PSI:Biology
New York Structural Genomics Research Consortium
SmCD00544951
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1653nts
Open reading frame: 19 to 1671

CTGTACTTCCAATCCATGCCTGCAGCAACCACTTATGCGACGACTGGTAACGCCTATATC
GACGGTCTCCTTGGCGACTGGAAATGGGGGATCAAGGACTTCACGTTCAGTTTCCCAACC
AGCGCTTCATTCTACAGCGCCGGTTATGGCAATGGCGAGCCGCTCAAGGGCTTTGCGGCC
TTGAGCGGCGCACAGCAGGCTGCCACGCGAGCGGCACTTGATCAGTTTTCCTCCGTCGCC
AACGTGACATTCACCGAGATCACGGAGAGTGCGACCAAGCACGCCGATCTTCGCCTCGCC
TCGTCCGACGCTCCGAGCACCGCGTGGGCCTATTTCCCTTCGACCGCGGCAGAAGGCGGC
GACGCGTGGTTCAACAAATCATCTGGCCACTACAGCAGACCGGTGAAGGGAAACTACGCA
TATGTGACATTCCTTCACGAGACCGGCCACGCGCTTGGGCTGGAACACGCCCATGAGGGC
AACGTCATGCCGGTGAACCGCGACTCTATGGAATACACGGTCATGAGTTACCGCTCCTAT
GTCGGTGCCTCGACGACGACGGGCTATACCAACGAGACCTGGGGATACGCACAATCGCTG
ATGATGTACGATATCGCTGCCTTGCAGCATATGTACGGCGCGGATTTTACCACCCACAGC
GAAAATACGACATATCGCTGGAGCCCGACGTCAGGGGAGATGTTCGTCAATGGTATGGGT
CAGGGTGCTCCTGGCGGCAACAAGATCCTGCTTACGGTATGGGACGGCGGCGGAACGGAC
ACCTACGATTTCTCCAACTATACGACTGCACTGAAGGTCGATCTCCGCCCGGGCGAATGG
ACGACCACCTCGGCCGCGCAATTGGCGAAGCTGCACTACGACGGTTCGAAGGTGGCGATC
GGCAATATTGCCAACGCGCTACAGTATCAGGGCGACACGCGCTCCCTCATCGAGAACGCG
AAGGGTGGCGCAGGCAACGACGCGATCACCGGCAATGCTGCTGCAAATGCTCTTTGGGGT
AACGGCGGCAACGACAGGCTGATCGGCGGCGACGGGAACGACAATCTTGCCGGCGGTGCG
GGCGCCGACCGGCTCGACGGCGGCAATGGCACCGATCTGGCGAATTACTCCAATGCCACC
GCCGGCATGGTTGCGGATCTTTACTCGCCCGGTTCCAACACGGGGGAAGCGGCTGGCGAT
ACATATGTATCGATCGAGCGGCTCTATGGTTCAGCCTTCAACGACACCCTGCGCGGAGAC
AATCGCGCAAACCTCCTGAACGGCCTAGCAGGTAACGACATGCTGAATGGGCGTGACGGC
AACGACACCCTGATCGGCGGTAACGGCGCCGATAGGCTGATTGGCGGCGGCGGCGCGGAC
ACTTTTGTCTTTCAGACGACGGCGCAATCGGCGCCGGCTTTCAGGGACGTGATCGACGAT
TTCGCGTCCGGCGTTGATCGGATGGATTTGCGTTCAATCGACGCCAGCAGCAAGGCCATC
GGAGACCAGGCGTTTCTATTTATCGGCAGCAGTGCGTTCCACGGCAAAGCAGGTGAACTT
AACTTCAGGAGTGGGATAGTTTCCGGTGACGTCAATGGCGACGGCCTTGCAGATTTCCAA
ATCAGGGTGATGAATCTGTCGGCTCTTTCCGGAAGCGATTTCCTGCTTTAACAGTAAAGG

Protein sequence predicted based on nucleotide sequence

PAATTYATTGNAYIDGLLGDWKWGIKDFTFSFPTSASFYSAGYGNGEPLKGFAALSGAQQ
AATRAALDQFSSVANVTFTEITESATKHADLRLASSDAPSTAWAYFPSTAAEGGDAWFNK
SSGHYSRPVKGNYAYVTFLHETGHALGLEHAHEGNVMPVNRDSMEYTVMSYRSYVGASTT
TGYTNETWGYAQSLMMYDIAALQHMYGADFTTHSENTTYRWSPTSGEMFVNGMGQGAPGG
NKILLTVWDGGGTDTYDFSNYTTALKVDLRPGEWTTTSAAQLAKLHYDGSKVAIGNIANA
LQYQGDTRSLIENAKGGAGNDAITGNAAANALWGNGGNDRLIGGDGNDNLAGGAGADRLD
GGNGTDLANYSNATAGMVADLYSPGSNTGEAAGDTYVSIERLYGSAFNDTLRGDNRANLL
NGLAGNDMLNGRDGNDTLIGGNGADRLIGGGGADTFVFQTTAQSAPAFRDVIDDFASGVD
RMDLRSIDASSKAIGDQAFLFIGSSAFHGKAGELNFRSGIVSGDVNGDGLADFQIRVMNL
SALSGSDFLL*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYCGRC-020543
Recommended expression in:
Not Applicable