MCA1271 (M. capsulatus str. Bath )
In pNIC28-Bsa4 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MCA1271
Gene Name:  short chain dehydrogenase/reductase oxidoreductase
Original Clone ID:
14057
Keyword:
None
Species:
Methylococcus capsulatus str. Bath
Type:
cDNA
Vector Name:
pNIC28-Bsa4
Format: 
CLOSED
Source:
New York Structural Genomics Research Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Albert Einstein College of Medicine
Steven Almo
PSI:Biology
New York Structural Genomics Research Consortium
McCD00544959
Price: 
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 657nts
Open reading frame: 40 to 696

GTAGATCTGGGTACCGAGAACCTGTACTTCCAATCCATGGGCCGCACCGTCTTGATCACC
GGCGCCGGGGGCGATCTCGGCGCCGTCGCAGCCGAGGCCTGTGCCGGTGCCGGCGCCACG
GTGATCCTCCTGGGCCGTACCCTCGGACGGCTGGAAGCCACTTACGACCGCATCGTCCGG
CAGGGCGGCTCCGAACCACTCCTGGCGCCGCTGGACCTGGCCACCGCCACGCTCGAAGAA
TTGTCGGCCATCGCCGGCTCGATCGAATCCCGGCTCGGCGCATTGCATGGGCTGATCCAT
TGCGCCGCGCACATGGATTACCTGGGCCCTTTGCGGGATGTCGGCGACGCCCAGTGGCGC
GATACGCTCGACGTCAACCTGACCGGCGCGTTCCGCCTGACACGGACTCTGTTGCCCCTG
CTGACGCGCTCGGAAGCCGCCGCCGCGGTCTTCGTCAGTGACAGCGAAGCCGAGCGGCGG
CGTAGCTACTGGGGACCCTATGCCGTCGCCAAGGCCGGACTGGAAGCCTTCGCCCGGATT
CTCGCGGATGAATACGAAAGCGCCGGCACGCTCAGGGTCAACCTGCTCACGCCCGGCGCG
ATCCGCACCCGCCTGCGGCGCAAGGCATTTCCCACGGAAACCGCGCCGGAACCCGGGGCG
CTCGCCGCCCGTTTCGTCGACCTGATGCGCAGCTAACAGTAAAGGTAA

Protein sequence predicted based on nucleotide sequence

GRTVLITGAGGDLGAVAAEACAGAGATVILLGRTLGRLEATYDRIVRQGGSEPLLAPLDL
ATATLEELSAIAGSIESRLGALHGLIHCAAHMDYLGPLRDVGDAQWRDTLDVNLTGAFRL
TRTLLPLLTRSEAAAAVFVSDSEAERRRSYWGPYAVAKAGLEAFARILADEYESAGTLRV
NLLTPGAIRTRLRRKAFPTETAPEPGALAARFVDLMRS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
c51a,A17A;c144g,H48Q;

c444t,R148R;t516c,Y172Y
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYCGRC-014057
Recommended expression in:
Not Applicable