MM_0241 (M. mazei Go1 )
In pNIC28-Bsa4 (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  MM_0241
Gene Name:  hypothetical protein
Original Clone ID:
19052
Keyword:
None
Species:
Methanosarcina mazei Go1
Type:
cDNA
Vector Name:
pNIC28-Bsa4
Format: 
CLOSED
Source:
New York Structural Genomics Research Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Albert Einstein College of Medicine
Steven Almo
PSI:Biology
New York Structural Genomics Research Consortium
MmCD00545452
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1500nts
Open reading frame: 34 to 1533

CTGGGTACCGAGAACCTGTACTTCCAATCCATGGTAAAAAAATCAATTCATGAGATTAAT
AAAAAAATTGAAGATGGGAGCGTCAATGTAGTCACAGCCGAAGAAATGGTTGGAATTGTT
GAAGACCTCGGCGTGGAAAGTGCTGCGAAAGAAGTGGATGTGGTGACAACAGGCACTTTC
GGAGCCATGTGCTCATCAGGTTTGATGCTCAATCTGGGTCATTCAGAACCCCCGATTAAA
ATTCAGAAATTATGGTTCAATAATGTTGAGGCGTACAGCGGGATTGCTGCTGTTGATGCT
TACCTCGGAGCTGCCCAGATCTCGGACACACGCGGGATTCAGTATGGAGGAGCACACGTT
ATTGAAGACCTTCTCAGGGGAAAAGAAATCAATGTTCATGCAACTTCCTACGGGACAGAC
TGCTACCCGAGAAAAGTGCTTGATACGGCGATCACCCTTGAAGACTTAAACGAAGCCGTT
CTTCTCAACCCCAGGAACGCATACCAGAAATACAACGCTGCAACAAACAGTTCTAAAAGG
ATCCTGAATACTTATATGGGTGAGCTTCTGCCCAACTTCGGAAACGTCACTTATTCCGGA
GCAGGAGTGCTTTCTCCCCTGTCAAACGACCCGGGATATGAAACTATAGGAACAGGCACA
AGGATTTTTCTTGGAGGTGCGCAGGGTTATGTCATAGGCAACGGGACTCAGCATTCCCCA
TCCGGAAGTTTTGGAACCCTCATGCTTAAAGGGAACCTTAAGGAAATGAGCCCTGATTAT
TTAAGAGCCGCCTCTTTTGCAGGTTACGGGACAACCCTTTACATGGGAATCGGGATTCCT
ATTCCTATCCTGAATGAAAAGCTTGCAGCTGCAACTGCAGTACGTGACGAGGACATCACC
ACCAGTGTGCTTGACTATGCTATCGGAAGCAGGGACAAGCCTGTGGTCAGGCAGGTAAAT
TATGCCGAACTCAGATCTGGATCTGTGCAGATTGAAGGAAAGGATGTTCCAACATCTTCC
CTTTCAAGCTTCAAGAACGCCAGAAAGATTGCAAATGAGCTGAAGGAATGGGTAAAACAC
GGAAAGTTCTTTGTCAGCATGCCTGTGGAAAAGCTTTGCAGTGAAGGCTCGGCAAAGCCC
ATGAAGCAGACTCAGGCAGTGCCTCTTGTAAAAGACGTTATGTCCGATTTCATCGTCACT
ATCAAAAAGGATCAGACTGTCCAGGACGCTGCAAAGAAAATCTGGGAGAACTCTTTCAAC
CACCTTGCAGTAGTTTCGGATACCGGTGAACTTGTAGGGATCCTCACAGCCTGGGATATC
TCAAAGGCAGTCGCGGAAAACATTTTCGATTCCGTAGAAAGCGTTATGACGAAAAAGGTC
CTTACCTGCGCCCCTAACGAACCTGTGGACCTTGCAGCCCGCAGGCTTGACCGCTACGGA
GTTTCAGCAATGCCGGTGATTGATGCGCAGAAGAAAGTACTCGGAATTATCACAAGCGAC
AATATAAGCAAGCTCTTTGCAAGGAGGTACTAACAGTAAAGGTGGATACGGATCCG

Protein sequence predicted based on nucleotide sequence

VKKSIHEINKKIEDGSVNVVTAEEMVGIVEDLGVESAAKEVDVVTTGTFGAMCSSGLMLN
LGHSEPPIKIQKLWFNNVEAYSGIAAVDAYLGAAQISDTRGIQYGGAHVIEDLLRGKEIN
VHATSYGTDCYPRKVLDTAITLEDLNEAVLLNPRNAYQKYNAATNSSKRILNTYMGELLP
NFGNVTYSGAGVLSPLSNDPGYETIGTGTRIFLGGAQGYVIGNGTQHSPSGSFGTLMLKG
NLKEMSPDYLRAASFAGYGTTLYMGIGIPIPILNEKLAAATAVRDEDITTSVLDYAIGSR
DKPVVRQVNYAELRSGSVQIEGKDVPTSSLSSFKNARKIANELKEWVKHGKFFVSMPVEK
LCSEGSAKPMKQTQAVPLVKDVMSDFIVTIKKDQTVQDAAKKIWENSFNHLAVVSDTGEL
VGILTAWDISKAVAENIFDSVESVMTKKVLTCAPNEPVDLAARRLDRYGVSAMPVIDAQK
KVLGIITSDNISKLFARRY*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
SolubleProtein: Not_Applicable
ProteinExpressed: Tested_Not_Found
ProteinPurified: Tested_Not_Purified
Find experimental protocols in NYCGRC-019052
Recommended expression in:
Not Applicable