Original Clone ID:
92944
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSpeedET
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
The Scripps Research Institute
PSI:Biology
Joint Center for Structural Genomics
PSI:Biology
Joint Center for Structural Genomics
HsCD00545607
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 420nts
Open reading frame: 51 to 470
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCCACACTGAGA
AGAGGGGGCGGATTTACCTAAAGGCTGAGGTTGCTGATGAAAAGCTCCATGTCACAGTAC
GAGATGCAAAAAATCTAATCCCTATGGATCCAAACGGGCTTTCAGATCCTTATGTGAAGC
TGAAACTTATTCCTGATCCCAAGAATGAAAGCAAGCAAAAAACCAAAACCATCCGCTCCA
CACTAAATCCGCAGTGGAATGAGTCCTTTACATTCAAATTGAAACCTTCAGACAAAGACC
GACGACTGTCTGTAGAAATCTGGGACTGGGATCGAACAACAAGGAATGACTTCATGGGAT
CCCTTTCCTTTGGAGTTTCGGAGCTGATGAAGATGCCGGCCAGTGGATGGTACAAGTTGC
TTAACCAAGAAGAAGGTGAGTACTACAACGTACCCATTCCGGAAGGGTAACGCGACTTAA
TTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG
Protein sequence predicted based on nucleotide sequence
HTEKRGRIYLKAEVADEKLHVTVRDAKNLIPMDPNGLSDPYVKLKLIPDPKNESKQKTKT IRSTLNPQWNESFTFKLKPSDKDRRLSVEIWDWDRTTRNDFMGSLSFGVSELMKMPASGW YKLLNQEEGEYYNVPIPEG*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in 422958
Recommended expression in:
Not Applicable
Vector Name:
pSpeedET
Synonyms:
pSpeed-ET
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments:
If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp):
5464
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
PMID:
18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type origin of replication
3306
3988
inducible trxn activator protein gene
araC
araC coding sequence
4563
5438
negative selection marker
ccdB
ccdB death cassette gene (lost in with-insert form)
1596
1805
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
CmR
chloramphenicol resistance gene (CmR) (lost in with-insert form)
502
1158
selectable marker
KanR
kanamycin resistance gene
2396
3208
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
2258
2284
Gene
Gene Symbol :
PRKCA
Symbol Nomenclature :
PRKCA
SYNONYM :
AAG6; PKC-alpha; PKCA; PRKACA
Designation :
PKC-A|aging-associated gene 6|protein kinase C alpha type
Full Nomenclature :
protein kinase C, alpha
GENEID :
GI :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
a6b1 and a6b4 Integrin signaling
NCI :
ATF-2 transcription factor network
NCI :
Canonical NF-kappaB pathway
NCI :
Downstream signaling in naïve CD8+ T cells
NCI :
Endothelins
NCI :
ErbB1 downstream signaling
NCI :
IL8- and CXCR1-mediated signaling events
NCI :
IL8- and CXCR2-mediated signaling events
NCI :
mTOR signaling pathway
NCI :
PAR1-mediated thrombin signaling events
NCI :
PDGFR-beta signaling pathway
NCI :
Ras signaling in the CD4+ TCR pathway
NCI :
Regulation of Ras family activation
NCI :
Retinoic acid receptors-mediated signaling
NCI :
Role of Calcineurin-dependent NFAT signaling in lymphocytes
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
Syndecan-4-mediated signaling events
NCI :
TCR signaling in naïve CD4+ T cells
NCI :
TCR signaling in naïve CD8+ T cells
NCI :
Thromboxane A2 receptor signaling
NCI :
VEGFR1 specific signals
Panther :
5HT2 type receptor mediated signaling pathway
Panther :
Alpha adrenergic receptor signaling pathway
Panther :
Alzheimer disease-amyloid secretase pathway
Panther :
Angiogenesis
Panther :
Angiotensin II-stimulated signaling through G proteins and beta-arrestin
Panther :
Apoptosis signaling pathway
Panther :
EGF receptor signaling pathway
Panther :
Endothelin signaling pathway
Panther :
FGF signaling pathway
Panther :
Heterotrimeric G-protein signaling pathway-Gq alpha and Go alpha mediated pathway
Panther :
Histamine H1 receptor mediated signaling pathway
Panther :
Muscarinic acetylcholine receptor 1 and 3 signaling pathway
Panther :
Oxytocin receptor mediated signaling pathway
Panther :
PDGF signaling pathway
Panther :
Thyrotropin-releasing hormone receptor signaling pathway
Panther :
VEGF signaling pathway
Panther :
Wnt signaling pathway
Reactome :
Acetylcholine regulates insulin secretion
Reactome :
beta-catenin independent WNT signaling
Reactome :
Ca-dependent events
Reactome :
Ca2+ pathway
Reactome :
Calmodulin induced events
Reactome :
CaM pathway
Reactome :
Cell Cycle
Reactome :
Cell Cycle, Mitotic
Reactome :
DAG and IP3 signaling
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Depolymerisation of the Nuclear Lamina
Reactome :
Disease
Reactome :
Diseases associated with visual transduction
Reactome :
Disinhibition of SNARE formation
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling of activated FGFR
Reactome :
EGFR interacts with phospholipase C-gamma
Reactome :
EGFR Transactivation by Gastrin
Reactome :
Extracellular matrix organization
Reactome :
G alpha (z) signalling events
Reactome :
G-protein mediated events
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
Gene Expression
Reactome :
Glutamate Binding, Activation of AMPA Receptors and Synaptic Plasticity
Reactome :
GPCR downstream signaling
Reactome :
Hemostasis
Reactome :
HuR stabilizes mRNA
Reactome :
Immune System
Reactome :
Inactivation, recovery and regulation of the phototransduction cascade
Reactome :
Innate Immune System
Reactome :
Integration of energy metabolism
Reactome :
M Phase
Reactome :
Metabolism
Reactome :
Metabolism of mRNA
Reactome :
Metabolism of RNA
Reactome :
Mitotic M-M/G1 phases
Reactome :
Mitotic Prophase
Reactome :
Neuronal System
Reactome :
Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
Non-integrin membrane-ECM interactions
Reactome :
Nuclear Envelope Breakdown
Reactome :
Opioid Signalling
Reactome :
PCP/CE pathway
Reactome :
Phospholipase C-mediated cascade
Reactome :
Platelet activation, signaling and aggregation
Reactome :
PLC beta mediated events
Reactome :
PLC-gamma1 signalling
Reactome :
PLCG1 events in ERBB2 signaling
Reactome :
Regulation of insulin secretion
Reactome :
Regulation of KIT signaling
Reactome :
Regulation of mRNA stability by proteins that bind AU-rich elements
Reactome :
Response to elevated platelet cytosolic Ca2+
Reactome :
Signal Transduction
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by GPCR
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by Wnt
Reactome :
Signalling by NGF
Reactome :
Syndecan interactions
Reactome :
The phototransduction cascade
Reactome :
Trafficking of AMPA receptors
Reactome :
Trafficking of GluR2-containing AMPA receptors
Reactome :
Transmission across Chemical Synapses
Reactome :
Visual phototransduction
Reactome :
WNT5A-dependent internalization of FZD4
WikiPathway :
Alpha 6 Beta 4 signaling pathway
WikiPathway :
angiogenesis overview
WikiPathway :
Calcium Regulation in the Cardiac Cell
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
ErbB signaling pathway
WikiPathway :
FSH signaling pathway
WikiPathway :
G Protein Signaling Pathways
WikiPathway :
Insulin Signaling
WikiPathway :
Keap1-Nrf2 Pathway
WikiPathway :
Kit receptor signaling pathway
WikiPathway :
miRs in Muscle Cell Differentiation
WikiPathway :
Myometrial Relaxation and Contraction Pathways
WikiPathway :
TOR signaling
WikiPathway :
Wnt Signaling Pathway
WikiPathway :
Wnt Signaling Pathway and Pluripotency
Protein
PDB - Structural Annotation :
SMART domain :
Structural Annotation Wiki :
HPRD :
01498
PDB ID
Style
Color |
Clone
Cloning Information :
Original Clone ID :
92944
Experimental
Structural Biology Knowledgebase :
TargetTrack - Experimental data :
422958
Organism
Species Specific ID:
Genome
Reagents
Labome :