P4HA1 (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  P4HA1
Gene Name:  prolyl 4-hydroxylase, alpha polypeptide I
Original Clone ID:
85577
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Joint Center for Structural Genomics
HsCD00545912
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1605nts
Open reading frame: 51 to 1655

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGATCTGGT
ATATATTAATTATAGGAATTCTGCTTCCCCAGTCTTTGGCTCATCCAGGCTTTTTTACTT
CAATTGGTCAGATGACTGATTTGATCCATACTGAGAAAGATCTGGTGACTTCTCTGAAAG
ATTATATTAAGGCAGAAGAGGACAAGTTAGAACAAATAAAAAAATGGGCAGAGAAGTTAG
ATCGGCTAACTAGTACAGCGACAAAAGATCCAGAAGGATTTGTTGGGCATCCAGTAAATG
CATTCAAATTAATGAAACGTCTGAATACTGAGTGGAGTGAGTTGGAGAATCTGGTCCTTA
AGGATATGTCAGATGGCTTTATCTCTAACCTAACCATTCAGAGACAGTACTTTCCTAATG
ATGAAGATCAGGTTGGGGCAGCCAAAGCTCTGTTACGTCTCCAGGATACCTACAATTTGG
ATACAGATACCATCTCAAAGGGTAATCTTCCAGGAGTGAAACACAAATCTTTTCTAACGG
CTGAGGACTGCTTTGAGTTGGGCAAAGTGGCCTATACAGAAGCAGATTATTACCATACGG
AACTGTGGATGGAACAAGCCCTAAGGCAACTGGATGAAGGCGAGATTTCTACCATAGATA
AAGTCTCTGTTCTAGATTATTTGAGCTATGCGGTATATCAGCAGGGAGACCTGGATAAGG
CACTTTTGCTCACAAAGAAGCTTCTTGAACTAGATCCTGAACATCAGAGAGCTAATGGTA
ACTTAAAATATTTTGAGTATATAATGGCTAAAGAAAAAGATGTCAATAAGTCTGCTTCAG
ATGACCAATCTGATCAGAAAACTACACCAAAGAAAAAAGGGGTTGCTGTGGATTACCTGC
CAGAGAGACAGAAGTACGAAATGCTGTGCCGTGGGGAGGGTATCAAAATGACCCCTCGGA
GACAGAAAAAACTCTTTTGCCGCTACCATGATGGAAACCGTAATCCTAAATTTATTCTGG
CTCCAGCTAAACAGGAGGATGAATGGGACAAGCCTCGTATTATTCGCTTCCATGATATTA
TTTCTGATGCAGAAATTGAAATCGTCAAAGACCTAGCAAAACCAAGGCTGAGGCGAGCCA
CCATTTCAAACCCAATAACAGGAGACTTGGAGACGGTACATTACAGAATTAGCAAAAGTG
CCTGGCTCTCTGGCTATGAAAATCCTGTGGTGTCTCGAATTAATATGAGAATACAAGATC
TAACAGGACTAGATGTTTCCACAGCAGAGGAATTACAGGTAGCAAATTATGGAGTTGGAG
GACAGTATGAACCCCATTTTGACTTTGCACGGAAAGATGAGCCAGATGCTTTCAAAGAGC
TGGGGACAGGAAATAGAATTGCTACATGGCTGTTTTATATGAGTGATGTGTCTGCAGGAG
GAGCCACTGTTTTTCCTGAAGTTGGAGCTAGTGTTTGGCCCAAAAAAGGAACTGCTGTTT
TCTGGTATAATCTGTTTGCCAGTGGAGAAGGAGATTATAGTACACGGCATGCAGCCTGTC
CAGTGCTAGTTGGCAACAAATGGGTATCCAATAAATGGCTCCATGAACGTGGACAAGAAT
TTCGAAGACCTTGTACGTTGTCAGAATTGGAATAACGCGACTTAATTAACTCGTTTAAAC
GGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWA
EKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQY
FPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADY
YHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQR
ANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKM
TPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRL
RRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANY
GVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKG
TAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 421244
Recommended expression in:
Not Applicable