CC_2852 (C. vibrioides )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  CC_2852
Gene Name:  sensory box histidine kinase/response regulator
Original Clone ID:
CcR186-21.3
Keyword:
None
Species:
Caulobacter vibrioides
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI
Northeast Structural Genomics Consortium
CcCD00546453
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1959nts
Open reading frame: 51 to 2009

AATTTCCCCTCTAGAATAATTTTGTTTAACTTTAAGAAGGAGATATACATATGAACGCCG
CTACTCTTGTGGACACACACGCCATCGACACCGCGCTGCAAGAGGCGTTCGAAGCCGCGC
CGGAAGGCTTTGCGCTGTTCGACGCCGAGGATCGCTGCGTTATCTGGAACGCCCGGTACG
GCGAACTGTGGTCCAAGTGGGGCGTCACCCTGGCGGTGGGGGCCACGTTCGAGAGCCTGC
TGCTGGCGGGCCTGGCGGCGGGGCGCTTTCCCGAGGCCGATGGCGATCGCGCGGCCTGGC
TGGCCCACCAGCTGGGCCGGCGCAAGCTGGACCGCATCGAAGAGATCCGCGAAGAGTTCG
GCGAATATCTACGGTTCGAGAACCGGCGCACGCCCGCCGGCGGGCTCATCACCACCTGCG
CCAACCTCACCAACCTGCGTCGCCGCGAGGCCCAGGCCCAGGCGGCCCGCAGCTTTCTGG
ACACCATTGTCGAGAACACGCCGGCCATGCTGTTCGTCAAGGACGGCGATACGGGCGCCT
TCCTGTTGGTCAATCGGGCCGCCGAGAATCAGCTGGGCGTGCCGCGCGCCGAGCTCCTGG
GCAAGAGCGACTACGACTTCTTTCCCAAGGAGCAGGCCGACGCCTTCGCCGCCGACGACC
GCCGGGTGATCGCAAGCGGGAAGCTGGTCACCATCGACGAAGAGCCGCTGGATACGCCCC
ACAATGGTCGCCGCTGGTTGCAGACCCGCAAGTTCGCCATCCCGGGCGAGGACGGCCGCC
GCCACCTGCTGGTGATCTGCGAGGACATCACCGAGCGCAAGGCCGGCGCCGCGGCCCTGG
CCGAGGCCCTGCAGAAGGCCGAGGCGGGCAGCGTCGCCAAGAGCGAGTTCCTGGCCAATA
TGAGCCACGAAATCCGCACGCCCCTGAACGGGGTGATCGGCATGGCCGAGGTGCTGAGCC
GCACCGCCCTGGACGACGCCCAGCGCGAGATGATGGAGGTGATCCTCAATTCCGGCCGGG
CGCTGAACCTGCTGCTCAGCGACATTCTCGACCTGTCCAAGATCGAAGCCGGCGGGCTTG
AGCTGTCGCGCGACCCGCTGGACCTGCGCGAGGCGATCACCGCGGCGGTCGCCGTGTTCA
AGGCCGTCGCCGAGAGCAAGGGGCTGGTCTTCCGGCTGACCTTCGCCGACGACTTCCACG
ACCATGTCCTGGGCGACGCCCTGCGGGTGCGCCAGATCGTGGCCAACCTGACCAGCAACG
CCGTCAAGTTCACGACCGCCGGCTCGGTGATCATCGAGGCCCGGACCCTTGTCGCCGAAG
ACGGGCGGATCGACCTGACCGTCAGCGTCCAGGACACCGGCGAGGGGTTTGACGAGGACA
GCGCCGACCGATTGTTCGAGCGCTTCCAGCAGGCCGATGGTTCGGTGACGCGCAAGGTCG
GCGGCACCGGGCTTGGCCTGCCGATCGCCCGGCGCCTGGCTCGGCTGATGGACGGCGAGA
TCTCCTGCGTGGCCCATCCGGGCGAGGGGGCCACCTTCGTCTTCGAGGCCCTCTTCCTGG
CCGATGGTCAGCGCACGCCGGCGGCGTCCGCCGATCCGGCCGGCGAGCTGGCCCTGGATC
GTCGCCTGCGGGTGCTGCTGGCCGAGGACCATCCCACCAACCAGAAGGTGATCGGCCTGA
TGCTGGCGGACGCCGCCGACGTCACGGTGGCCGAGGACGGCCAGGCCGCCGTCGAAGCCT
TCGAGAAGCACGCCTATGACGTGGTGCTGATGGACACCCAGATGCCGGTCATGGACGGCC
TGGCCGCCATCGCCGAAATCCGCCGGCGCGAACGCGAGGCGCAGGCGCGCCGCACGCCGA
TCATCTCGCTGACCGCCAACGCCATGCCCCATCAGGTGCGCGCCTGCCTGGAGGCCGGCG
CGGATCTGCACCTGGCCAAGCCCGTCAGCATCCAGGGCCTCTTCGCCACGCTCGGCGCCG
CGCTGGAGACCCGTCCGGGCGGTGAGGGC

Protein sequence predicted based on nucleotide sequence

MNAATLVDTHAIDTALQEAFEAAPEGFALFDAEDRCVIWNARYGELWSKWGVTLAVGATF
ESLLLAGLAAGRFPEADGDRAAWLAHQLGRRKLDRIEEIREEFGEYLRFENRRTPAGGLI
TTCANLTNLRRREAQAQAARSFLDTIVENTPAMLFVKDGDTGAFLLVNRAAENQLGVPRA
ELLGKSDYDFFPKEQADAFAADDRRVIASGKLVTIDEEPLDTPHNGRRWLQTRKFAIPGE
DGRRHLLVICEDITERKAGAAALAEALQKAEAGSVAKSEFLANMSHEIRTPLNGVIGMAE
VLSRTALDDAQREMMEVILNSGRALNLLLSDILDLSKIEAGGLELSRDPLDLREAITAAV
AVFKAVAESKGLVFRLTFADDFHDHVLGDALRVRQIVANLTSNAVKFTTAGSVIIEARTL
VAEDGRIDLTVSVQDTGEGFDEDSADRLFERFQQADGSVTRKVGGTGLGLPIARRLARLM
DGEISCVAHPGEGATFVFEALFLADGQRTPAASADPAGELALDRRLRVLLAEDHPTNQKV
IGLMLADAADVTVAEDGQAAVEAFEKHAYDVVLMDTQMPVMDGLAAIAEIRRREREAQAR
RTPIISLTANAMPHQVRACLEAGADLHLAKPVSIQGLFATLGAALETRPGGEG

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g34a,V12I;a229g,N77D;

g332a,S111N;t410a,L137Q;

a703t,I235F
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in CcR186
Recommended expression in:
Not Applicable