GSU0036 (G. sulfurreducens )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  GSU0036
Gene Name:  poly-gamma-glutamate capsule biosynthesis protein
Original Clone ID:
GsR203-24-351-21.2
Keyword:
None
Species:
Geobacter sulfurreducens
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
Rutgers University
PSI
Northeast Structural Genomics Consortium
GsCD00546481
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 987nts
Open reading frame: 43 to 1029

TCTAGAATAATTTTGGTTTAACTTTAAGAAGGAGATATACATATGGAGCGCATCAGCCTT
TCTTTTGTGGGTGATGTGATGCTGGCCGGCAGCGCAACTGACACCCTCCAGCGGTACGGC
TACAGCTATCCGTTTTCCGCTACCGCCGCAGAGCTGCGACGTAGCGACCAGGTGGTGGGC
AACCTGGAAGCCCCCCTCACCGACGGGGGGCGCGAGTTCCGCGCCAAACGTTTCCGCTTC
AAGGCTTCTCCCGTCGCGGCAGCGGCCCTGAAACGGGCCGGCTTCTCGGTCATGACACTG
GCCAATAACCACATGATGGACTTTGGCGCCGATGGGTTGAGCGACACGATTCACCATCTC
AATCGTAACGGCATCGCCTTCGCAGGCGCTGGGCCGTCAATTGCTGATGCCCGACGCGAA
GCATCCGTGACGGTCAGGGGGCAGACCGTCGCCTTCCTGGCCTACTCGCTCACCCAGCCG
ATCGAATTCTTCGCCACAGAGGGACGTCCTGGCACCGCACCCGGCTACGCAGGCCACTAT
CTCGCGGATATCCGACGGGTCCGCAGCAGCGCCGATCATGTGGTCGTCTCCTTCCACTGG
GGACAGGAGCGCGCCGCGCTACCATCGCCCTACCAGATCGAAACAGCCCATCGCGCTATC
GATGTAGGGGCTGACATCGTCATCGGCCACCATCCCCATGTCCTCCAGGGAATCGAAATC
TATCGCGGCAGCCCGATCTTTTACAGCCTCGGTAACTTTGCCTTCGGCAGCCGGAGCCCC
TCGGCAGACCGGAGCATCATCGCCCGGGTGACCCTCGGCGAAGGACCGCCGATTGTGGAG
GTCATCCCTCTCAACGTTCTTTTCCGCGAGGTACGCTTTCAACCGGCCATCCTCACGGGC
CGTAAGGCGGCGGACGTAGTGGACCGGCTGAATCGTCTGTCAGCCCCCTTTAGCACGGTC
ATCACCTCCACCGCTGGCAGCCACCTCGTCGCGCCTGCGGAGGCTAATGCCCGGCTTGCC
CGCAGGCAGCTCGAGCACCACCACCACCACCACTGAGATCCGGCTGCTAACAAAGCCCGA

Protein sequence predicted based on nucleotide sequence

MERISLSFVGDVMLAGSATDTLQRYGYSYPFSATAAELRRSDQVVGNLEAPLTDGGREFR
AKRFRFKASPVAAAALKRAGFSVMTLANNHMMDFGADGLSDTIHHLNRNGIAFAGAGPSI
ADARREASVTVRGQTVAFLAYSLTQPIEFFATEGRPGTAPGYAGHYLADIRRVRSSADHV
VVSFHWGQERAALPSPYQIETAHRAIDVGADIVIGHHPHVLQGIEIYRGSPIFYSLGNFA
FGSRSPSADRSIIARVTLGEGPPIVEVIPLNVLFREVRFQPAILTGRKAADVVDRLNRLS
APFSTVITSTAGSHLVAPAEANARLARRQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t128a,L43Q;c456a,T152T;

c623t,A208V
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in GsR203
Recommended expression in:
Not Applicable