AGT (Homo sapiens) in pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  AGT
Gene Name:  angiotensinogen (serpin peptidase inhibitor, clade A, member 8)
Original Clone ID:
99243
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI:Biology
Joint Center for Structural Genomics
HsCD00547348
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1458nts
Open reading frame: 51 to 1508

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGCGGAAGC
GAGCACCCCAGTCTGAGATGGCTCCTGCCGGTGTGAGCCTGAGGGCCACCATCCTCTGCC
TCCTGGCCTGGGCTGGCCTGGCTGCAGGTGACCGGGTGTACATACACCCCTTCCACCTCG
TCATCCACAATGAGAGTACCTGTGAGCAGCTGGCAAAGGCCAATGCCGGGAAGCCCAAAG
ACCCCACCTTCATACCTGCTCCAATTCAGGCCAAGACATCCCCTGTGGATGAAAAGGCCC
TACAGGACCAGCTGGTGCTAGTCGCTGCAAAACTTGACACCGAAGACAAGTTGAGGGCCG
CAATGGTCGGGATGCTGGCCAACTTCTTGGGCTTCCGTATATATGGCATGCACAGTGAGC
TATGGGGCGTGGTCCATGGGGCCACCGTCCTCTCCCCAACGGCTGTCTTTGGCACCCTGG
CCTCTCTCTATCTGGGAGCCTTGGACCACACAGCTGACAGGCTACAGGCAATCCTGGGTG
TTCCTTGGAAGGACAAGAACTGCACCTCCCGGCTGGATGCGCACAAGGTCCTGTCTGCCC
TGCAGGCTGTACAGGGCCTGCTAGTGGCCCAGGGCAGGGCTGATAGCCAGGCCCAGCTGC
TGCTGTCCACGGTGGTGGGCGTGTTCACAGCCCCAGGCCTGCACCTGAAGCAGCCGTTTG
TGCAGGGCCTGGCTCTCTATACCCCTGTGGTCCTCCCACGCTCTCTGGACTTCACAGAAC
TGGATGTTGCTGCTGAGAAGATTGACAGGTTCATGCAGGCTGTGACAGGATGGAAGACTG
GCTGCTCCCTGACGGGAGCCAGTGTGGACAGCACCCTGGCTTTCAACACCTACGTCCACT
TCCAAGGGAAGATGAAGGGCTTCTCCCTGCTGGCCGAGCCCCAGGAGTTCTGGGTGGACA
ACAGCACCTCAGTGTCTGTTCCCATGCTCTCTGGCATGGGCACCTTCCAGCACTGGAGTG
ACATCCAGGACAACTTCTCGGTGACTCAAGTGTCCTTCACTGAGAGCGCCTGCCTGCTGC
TGATCCAGCCTCACTATGCCTCTGACCTGGACAAGGTGGAGGGTCTCACTTTCCAGCAAA
ACTCCCTCAACTGGATGAAGAAACTGTCTCCCCGGACCATCCACCTGACCATGCCCCAAC
TGGTGCTGCAAGGATCTTATGACCTGCAGGACCTGCTCGCCCAGGCTGAGCTGCCCGCCA
TTCTGCACACCGAGCTGAACCTGCAAAAATTGAGCAATGACCGCATCAGGGTGGGGGAGG
TGCTGAACAGCATTTTTTTTGAGCTTGAAGCGGATGAGAGAGAGCCCACAGAGTCTACCC
AACAGCTTAACAAGCCTGAGGTCTTGGAGGTGACCCTGAACCGCCCATTCCTGTTTGCTG
TGTATGATCAAAGCGCCACTGCCCTGCACTTCCTGGGCCGCGTGGCCAACCCGCTGAGCA
CAGCATAACGCGACTTAATTAACTCGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG

Protein sequence predicted based on nucleotide sequence

MRKRAPQSEMAPAGVSLRATILCLLAWAGLAAGDRVYIHPFHLVIHNESTCEQLAKANAG
KPKDPTFIPAPIQAKTSPVDEKALQDQLVLVAAKLDTEDKLRAAMVGMLANFLGFRIYGM
HSELWGVVHGATVLSPTAVFGTLASLYLGALDHTADRLQAILGVPWKDKNCTSRLDAHKV
LSALQAVQGLLVAQGRADSQAQLLLSTVVGVFTAPGLHLKQPFVQGLALYTPVVLPRSLD
FTELDVAAEKIDRFMQAVTGWKTGCSLTGASVDSTLAFNTYVHFQGKMKGFSLLAEPQEF
WVDNSTSVSVPMLSGMGTFQHWSDIQDNFSVTQVSFTESACLLLIQPHYASDLDKVEGLT
FQQNSLNWMKKLSPRTIHLTMPQLVLQGSYDLQDLLAQAELPAILHTELNLQKLSNDRIR
VGEVLNSIFFELEADEREPTESTQQLNKPEVLEVTLNRPFLFAVYDQSATALHFLGRVAN
PLSTA*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
t803c,M268T;c1003t,P335S;

a1116g,L372L
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 422920
Recommended expression in:
Not Applicable