Original Clone ID:
99023
Keyword:
None
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pSpeedET
Format:
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Joint Center for Structural Genomics
Joint Center for Structural Genomics
HsCD00547435
Price:
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AVAILABLE
No restriction
Special MTA: PSI
Special MTA: PSI
Insert sequence: 654nts
Open reading frame: 51 to 704
CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCATGGAAGCCA
TCGCCAAATATGACTTCAAAGCTACTGCAGACGACGAGCTGAGCTTCAAAAGGGGGGACA
TCCTCAAGGTTTTGAACGAAGAATGTGATCAGAACTGGTACAAGGCAGAGCTTAATGGAA
AAGACGGCTTCATTCCCAAGAACTACATAGAAATGAAACCACATCCGTGGTTTTTTGGCA
AAATCCCCAGAGCCAAGGCAGAAGAAATGCTTAGCAAACAGCGGCACGATGGGGCCTTTC
TTATCCGAGAGAGTGAGAGCGCTCCTGGGGACTTCTCCCTCTCTGTCAAGTTTGGAAACG
ATGTGCAGCACTTCAAGGTGCTCCGAGATGGAGCCGGGAAGTACTTCCTCTGGGTGGTGA
AGTTCAATTCTTTGAATGAGCTGGTGGATTATCACAGATCTACATCTGTCTCCAGAAACC
AGCAGATATTCCTGCGGGACATAGAACAGGTGCCACAGCAGCCGACATACGTCCAGGCCC
TCTTTGACTTTGATCCCCAGGAGGATGGAGAGCTGGGCTTCCGCCGGGGAGATTTTATCC
ATGTCATGGATAACTCAGACCCCAACTGGTGGAAAGGAGCTTGCCACGGGCAGACCGGCA
TGTTTCCCCGCAATTATGTCACCCCCGTGAACCGGAACGTCTAACGCGACTTAATTAACT
CGTTTAAACGGTCTCCAGCTTGGCTGTTTTGGCGG
Protein sequence predicted based on nucleotide sequence
MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPW FFGKIPRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFL WVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRG DFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
kanamycin
Bacterial Selection Condition:
50ug/mL kanamycin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 422465
Recommended expression in:
Not Applicable
Vector Name:
pSpeedET
Synonyms:
pSpeed-ET
Sequencing Primer:
Forward:
pBAD forward
Reverse:
alt_bad_rev
Description:
Bacterial expression vector, arabinose or T7 induced expression, adds N-terminal MGSDKIHHHHHHENLYFQG tag; kanamycin resistance; PIPE cloning.
Comments:
If this vector contains an insert, the ccdB death cassette will not be present.
Size (bp):
5464
Parent Vector:
None
Properties:
PIPE cloning, bacterial expression, in vitro transcription, inducible expression, with tag/fusion/marker
Author Name:
Joint Center for Structural Genomics
Publications:
PMID:
18004753
Title: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ori
ColE1-type origin of replication
3306
3988
inducible trxn activator protein gene
araC
araC coding sequence
4563
5438
negative selection marker
ccdB
ccdB death cassette gene (lost in with-insert form)
1596
1805
promoter
T7
T7 promoter
290
310
promoter
araC
araC promoter
256
285
protease cleavage site
TEV
TEV protease cleavage site
382
402
selectable marker
CmR
chloramphenicol resistance gene (CmR) (lost in with-insert form)
502
1158
selectable marker
KanR
kanamycin resistance gene
2396
3208
tag
His
N-terminal 6xHis tag
364
381
trxn regulatory element
arabinose O1 operator
arabinose O1 operator
161
182
trxn regulatory element
arabinose O2 operator
arabinose O2 operator
5
23
trxn termination sequence
rrnB
rrnB T2 transcription terminator
2258
2284
Gene
Gene Symbol :
GRB2
Symbol Nomenclature :
GRB2
SYNONYM :
ASH; EGFRBP-GRB2; Grb3-3; MST084; MSTP084; NCKAP2
Designation :
HT027|SH2/SH3 adapter GRB2|abundant SRC homology|epidermal growth factor receptor-binding protein GRB2|growth factor receptor-bound protein 3|protein Ash
Full Nomenclature :
growth factor receptor-bound protein 2
GENEID :
GI :
GenBank Accession :
HGNC :
MIM :
Vega :
Target GenBank:
Pathways
NCI :
a6b1 and a6b4 Integrin signaling
NCI :
Angiopoietin receptor Tie2-mediated signaling
NCI :
BCR signaling pathway
NCI :
EGF receptor (ErbB1) signaling pathway
NCI :
EGFR-dependent Endothelin signaling events
NCI :
EPHA2 forward signaling
NCI :
EPHB forward signaling
NCI :
EPO signaling pathway
NCI :
ErbB1 downstream signaling
NCI :
ErbB2/ErbB3 signaling events
NCI :
ErbB4 signaling events
NCI :
Fc-epsilon receptor I signaling in mast cells
NCI :
FGF signaling pathway
NCI :
GMCSF-mediated signaling events
NCI :
IGF1 pathway
NCI :
IL2 signaling events mediated by PI3K
NCI :
IL2 signaling events mediated by STAT5
NCI :
IL2-mediated signaling events
NCI :
IL3-mediated signaling events
NCI :
IL4-mediated signaling events
NCI :
IL5-mediated signaling events
NCI :
IL6-mediated signaling events
NCI :
Insulin Pathway
NCI :
Internalization of ErbB1
NCI :
Nephrin/Neph1 signaling in the kidney podocyte
NCI :
Neurotrophic factor-mediated Trk receptor signaling
NCI :
PDGFR-alpha signaling pathway
NCI :
PDGFR-beta signaling pathway
NCI :
Plasma membrane estrogen receptor signaling
NCI :
Regulation of Ras family activation
NCI :
SHP2 signaling
NCI :
Signaling events mediated by focal adhesion kinase
NCI :
Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met)
NCI :
Signaling events mediated by PTP1B
NCI :
Signaling events mediated by Stem cell factor receptor (c-Kit)
NCI :
Signaling events mediated by TCPTP
NCI :
Signaling events mediated by VEGFR1 and VEGFR2
NCI :
Signaling events regulated by Ret tyrosine kinase
NCI :
TCR signaling in naïve CD4+ T cells
NCI :
TCR signaling in naïve CD8+ T cells
NCI :
TGF-beta receptor signaling
NCI :
Trk receptor signaling mediated by PI3K and PLC-gamma
NCI :
VEGFR3 signaling in lymphatic endothelium
Panther :
Angiogenesis
Panther :
B cell activation
Panther :
PDGF signaling pathway
Panther :
Ras Pathway
Reactome :
Adaptive Immune System
Reactome :
Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
Reactome :
Axon guidance
Reactome :
CD28 co-stimulation
Reactome :
CD28 dependent Vav1 pathway
Reactome :
Cell surface interactions at the vascular wall
Reactome :
Cell-Cell communication
Reactome :
Constitutive PI3K/AKT Signaling in Cancer
Reactome :
Costimulation by the CD28 family
Reactome :
Cytokine Signaling in Immune system
Reactome :
DAP12 interactions
Reactome :
DAP12 signaling
Reactome :
Developmental Biology
Reactome :
Disease
Reactome :
Downstream signal transduction
Reactome :
Downstream signaling events of B Cell Receptor (BCR)
Reactome :
Downstream signaling of activated FGFR
Reactome :
EGFR downregulation
Reactome :
EGFR Transactivation by Gastrin
Reactome :
Fc epsilon receptor (FCERI) signaling
Reactome :
FCERI mediated Ca+2 mobilization
Reactome :
FCERI mediated MAPK activation
Reactome :
Fcgamma receptor (FCGR) dependent phagocytosis
Reactome :
FRS2-mediated cascade
Reactome :
GAB1 signalosome
Reactome :
Gastrin-CREB signalling pathway via PKC and MAPK
Reactome :
GRB2 events in EGFR signaling
Reactome :
GRB2 events in ERBB2 signaling
Reactome :
GRB2:SOS provides linkage to MAPK signaling for Integrins
Reactome :
Hemostasis
Reactome :
IGF1R signaling cascade
Reactome :
Immune System
Reactome :
Innate Immune System
Reactome :
Insulin receptor signalling cascade
Reactome :
Integrin alphaIIb beta3 signaling
Reactome :
Interleukin receptor SHC signaling
Reactome :
Interleukin-2 signaling
Reactome :
Interleukin-3, 5 and GM-CSF signaling
Reactome :
IRS-mediated signalling
Reactome :
IRS-related events
Reactome :
IRS-related events triggered by IGF1R
Reactome :
NCAM signaling for neurite out-growth
Reactome :
Negative regulation of FGFR signaling
Reactome :
NGF signalling via TRKA from the plasma membrane
Reactome :
PI-3K cascade
Reactome :
PI3K Cascade
Reactome :
PI3K events in ERBB2 signaling
Reactome :
PI3K events in ERBB4 signaling
Reactome :
PI3K/AKT activation
Reactome :
PI3K/AKT Signaling in Cancer
Reactome :
PIP3 activates AKT signaling
Reactome :
Platelet activation, signaling and aggregation
Reactome :
Platelet Aggregation (Plug Formation)
Reactome :
Regulation of actin dynamics for phagocytic cup formation
Reactome :
Regulation of KIT signaling
Reactome :
Regulation of signaling by CBL
Reactome :
Role of LAT2/NTAL/LAB on calcium mobilization
Reactome :
SHC-mediated cascade
Reactome :
SHC-mediated signalling
Reactome :
SHC-related events
Reactome :
SHC-related events triggered by IGF1R
Reactome :
SHC1 events in EGFR signaling
Reactome :
SHC1 events in ERBB2 signaling
Reactome :
SHC1 events in ERBB4 signaling
Reactome :
Signal attenuation
Reactome :
Signal regulatory protein (SIRP) family interactions
Reactome :
Signal Transduction
Reactome :
Signaling by constitutively active EGFR
Reactome :
Signaling by EGFR
Reactome :
Signaling by EGFR in Cancer
Reactome :
Signaling by ERBB2
Reactome :
Signaling by ERBB4
Reactome :
Signaling by FGFR
Reactome :
Signaling by FGFR in disease
Reactome :
Signaling by FGFR mutants
Reactome :
Signaling by FGFR1 fusion mutants
Reactome :
Signaling by FGFR1 mutants
Reactome :
Signaling by GPCR
Reactome :
Signaling by Insulin receptor
Reactome :
Signaling by Interleukins
Reactome :
Signaling by PDGF
Reactome :
Signaling by SCF-KIT
Reactome :
Signaling by the B Cell Receptor (BCR)
Reactome :
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R)
Reactome :
Signalling by NGF
Reactome :
Signalling to ERKs
Reactome :
Signalling to RAS
Reactome :
SOS-mediated signalling
Reactome :
Spry regulation of FGF signaling
Reactome :
Tie2 Signaling
WikiPathway :
angiogenesis overview
WikiPathway :
B Cell Receptor Signaling Pathway
WikiPathway :
DNA damage response (only ATM dependent)
WikiPathway :
EGF/EGFR Signaling Pathway
WikiPathway :
EPO Receptor Signaling
WikiPathway :
ErbB signaling pathway
WikiPathway :
Focal Adhesion
WikiPathway :
IL-2 Signaling pathway
WikiPathway :
IL-3 Signaling Pathway
WikiPathway :
IL-4 signaling pathway
WikiPathway :
IL-5 signaling pathway
WikiPathway :
IL-6 signaling pathway
WikiPathway :
IL-9 signaling pathway
WikiPathway :
Insulin Signaling
WikiPathway :
Integrin-mediated cell adhesion
WikiPathway :
Leptin signaling pathway
WikiPathway :
MAPK signaling pathway
WikiPathway :
p38 MAPK Signaling Pathway
WikiPathway :
Prolactin Signaling Pathway
WikiPathway :
Signaling of Hepatocyte Growth Factor Receptor
WikiPathway :
TCR Signaling Pathway
WikiPathway :
TGF beta Signaling Pathway
WikiPathway :
TNF alpha Signaling Pathway
Protein
SMART domain :
SMART domain :
Structural Annotation Wiki :
HPRD :
00150
Clone
Cloning Information :
Original Clone ID :
99023
Experimental
TargetTrack - Experimental data :
422465
Organism
Species Specific ID:
Genome
Reagents
Labome :