BT_4645 (B. thetaiotaomicron VPI-5482 )
In pSpeedET (bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BT_4645
Gene Name:  hypothetical protein
Original Clone ID:
79568
Keyword:
partial cds
Species:
Bacteroides thetaiotaomicron VPI-5482
Type:
cDNA
Vector Name:
pSpeedET
Format: 
CLOSED
Source:
Joint Center for Structural Genomics
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
PSI:Biology
Joint Center for Structural Genomics
BtCD00547734
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AVAILABLE
No restriction
Special MTA: PSI
Insert sequence: 1473nts
Open reading frame: 51 to 1523

CTGATAAAATTCATCATCATCATCATCACGAAAACCTGTACTTCCAGGGCGACGGAGAAG
ATGTGCTGGAGCGTATGATCAGCCTGCCGAAGATGAAAGGAACGGTCTATTCACTGTTAG
GCAATATTTCTCAGCAGTCAGGTTATCTGTTTATCTATGACAGTAAAGTAGTAGATAATG
ATGTAACGGTGAAGATACGGAAAGGAGAGCGAACCATTCGTCAGGCTATTTATGAGATAA
CAGGAGATACCAGCCTGGAATTTAAAGTAATAGGGACTCATATTCTGATAACTTCTTCTT
CACCGACCAAACAGCAACAGGCGAAACCTTCATCCGTTCACCCTGTAAATCTGATGCTTA
CCGGAACTTTACTGGATAAGGAAACCGGAATGCCTGTTGCTTCTGCATCCGTTGGAGTCC
GGAGGACATCTGTGGGGATTGTTACTAATCAGGAAGGAGAGTTCAGACTTTCCTTACCGG
ATTCTTTGCAAAACGATTCGGTTGTTTTTTCTCATATAGGATATGTTTCACAAACTTTGG
AAGTTTCTGTTTTGGCAGGTCGCCATCATATTCTGAGCTTGGAACCAAAGGTTGTTCCTT
TACAGGAAGTAGTGGTTCAATGGGTCGATCCTTATAAGCTGCTGAAAGAAATGGGAAGGC
AAAGAGAGCAGAACTATTCTCATTCTCCTGCCTACCTGACCACATTTTACCGTGAAGGAG
TTTTATTGAAAAATAAGGTTCAGAATCTTACTGAAGCAGTTTTCAAAGTTTATAAGATCG
CTTCGCATTCTCCCGTTTCCGATCAGGCGAAGCTGTTGAAGATGAGTCGGCTGAGTAATG
TCGAAGCCAAAGACAGTCTGCTTGTAAAGGTGAAGTCCGGCATTCAGGCATGTTTTCAGA
TGGATATAATGAAGGATATGCCTTCATTCCTGATACCTGACGCAGGTGATAACGGTTATC
TGTATACTTCGCAGGGAGTGACATTCATAGACGACCGGTGTGTGAATGTCATACACTTTG
CACAAAAGAAGGAGATTATCGAGCCTCTCTATTGCGGAGATTTGTATATTGATGCCGAGA
CGAATGCCTTGTTACAAGCTCGTTTTGAGGTAGATCCCCAGCGTGTAAAGAAAGCGTCGG
AAATGTTTGTAGAAAGGCGCACGCATGGCATTCAGATAATACCGCAAAAGGTGGTTTATA
CTATTTCCTACAAACCATGGCAAGGTATCTATTATATTCATCATATCCGTGGAGACCTTT
CTTTCAAGGTGAAACGGCGACGGTTGCTGTCTGCTAGTCCGCAGATGCAGATCTGGTTTG
AAATGATCACTTGCAAAGTGGATACGGAACAAGTGACTGCATTCCCGCGGGCAGAAAGAT
TACCGACGCGTACTATCTTCTCTGATCTGAATTTCAAATATGATGAAGACTTCTGGAGGG
ATTTTAATGTGATACCTCTGGAAGAGGAACTTGGTAAGCTGATTGAGAGAATCTCTTTGA
AAATAGAAGAAATCGGCCCTTAACGCGACTTAATTAATTTGGCGGATGAGAGAAGATTTT
CAGCCT

Protein sequence predicted based on nucleotide sequence

DGEDVLERMISLPKMKGTVYSLLGNISQQSGYLFIYDSKVVDNDVTVKIRKGERTIRQAI
YEITGDTSLEFKVIGTHILITSSSPTKQQQAKPSSVHPVNLMLTGTLLDKETGMPVASAS
VGVRRTSVGIVTNQEGEFRLSLPDSLQNDSVVFSHIGYVSQTLEVSVLAGRHHILSLEPK
VVPLQEVVVQWVDPYKLLKEMGRQREQNYSHSPAYLTTFYREGVLLKNKVQNLTEAVFKV
YKIASHSPVSDQAKLLKMSRLSNVEAKDSLLVKVKSGIQACFQMDIMKDMPSFLIPDAGD
NGYLYTSQGVTFIDDRCVNVIHFAQKKEIIEPLYCGDLYIDAETNALLQARFEVDPQRVK
KASEMFVERRTHGIQIIPQKVVYTISYKPWQGIYYIHHIRGDLSFKVKRRRLLSASPQMQ
IWFEMITCKVDTEQVTAFPRAERLPTRTIFSDLNFKYDEDFWRDFNVIPLEEELGKLIER
ISLKIEEIGP*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition: 50ug/mL kanamycin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results:
ProteinExpressed: Not_Applicable
ProteinPurified: Not_Applicable
SolubleProtein: Not_Applicable
Find experimental protocols in 386483
Recommended expression in:
Not Applicable