Gene:
Gene Symbol:
No insert
Gene Name:
No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pET16-DEST
Source:
Kelley Moremen
Description:
Bacterial expression vector with a T7 promoter and a N-terminal 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning.
Comments:
Empty vector contains ccdB death cassette and Chloramphenicol resistance genes. Empty vector must be propagated in DB3.1 bacterial cells.
Publications:
None
Authors:
University of Georgia
Kelley Moremen
Kelley Moremen
EvNO00548203
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Vector Name:
pET16-DEST
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with a T7 promoter and a N-terminal 10xHis tag; ampicillin resistance in bacteria; Gateway recombinational cloning.
Comments:
None
Size (bp):
7428
Parent Vector:
None
Properties:
Gateway, acceptor (destination), bacterial expression, recombinational cloning, with tag/fusion/marker
Author Name:
University of Georgia
Kelley Moremen
Kelley Moremen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
death cassette
ccdB
ccdB death cassette (removed in clones with the insert)
6626
6931
promoter
T7
T7 promoter
5230
5245
protease cleavage site
Factor X
Factor X protease cleavage site
5365
5376
recombination site
attR1
Recombination site
5392
5516
recombination site
attR2
recombination site
6972
7096
repressor protein gene
LacI
Lac repressor coding sequence
3761
4720
selectable marker
CmR
chloramphenicol resistance (removed in clones with the insert)
5625
6284
selectable marker
AmpR
ampicillin resistance gene
209
1843
tag
His
N-terminal 10xHis tag
5323
5352
trxn regulatory element
lac operator
lac operator
5249
5273
trxn termination sequence
T7 term
T7 terminator
7155
7216
