DNASU Plasmid | ABCD4 (Homo sapiens) in pPICZa

Explanation of Terms

Gene:

Gene Symbol: ABCD4

Gene Name: ATP-binding cassette, sub-family D (ALD), member 4

Original Clone ID:

TPDB-0094

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pPICZa

Format:

FUSION

Source:

Center for the X-ray Structure Determination of Human Transporters

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Center for the X-ray Structure Determination of Human Transporters

HsCD00583074

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1815nts

Open reading frame: 20 to 1834

Reference Sequence Alignment

CGATGACGATAAGTCTAGAGCGGTCGCGGGGCCCGCGCCCGGAGCTGGCGCCAGGCCCAG
GTTAGATCTGCAATTTCTCCAGCGGTTCCTGCAGATACTGAAGGTTTTGTTTCCTTCTTG
GTCATCACAAAATGCCTTGATGTTCCTGACCCTTTTGTGCCTGACCCTACTGGAGCAATT
TGTGATCTACCAGGTTGGCCTGATCCCCAGTCAGTACTATGGGGTCCTGGGAAACAAAGA
CTTGGAAGGGTTTAAGACTCTGACATTCCTGGCTGTCATGCTCATTGTTCTGAACTCCAC
GCTGAAGAGCTTTGATCAGTTCACCTGCAACCTGCTGTATGTGAGCTGGAGGAAGGACCT
CACTGAGCACCTTCACCGCCTCTACTTCCGGGGCCGTGCGTACTACACCCTCAACGTGCT
GCGGGATGACATCGATAACCCGGACCAGCGCATCAGCCAGGACGTGGAGCGATTCTGCCG
GCAGCTCAGCAGCATGGCCAGCAAGCTCATCATCTCCCCGTTCACCCTCGTCTACTACAC
TTACCAGTGCTTCCAAAGCACAGGCTGGCTCGGGCCTGTGAGCATCTTCGGGTATTTCAT
CCTGGGGACCGTGGTGAACAAAACTTTGATGGGCCCCATTGTGATGAAGCTGGTGCATCA
GGAGAAGCTGGAGGGAGATTTTAGGTTCAAGCACATGCAGATTCGGGTGAATGCGGAGCC
TGCTGCTTTCTACAGAGCTGGGCATGTGGAGCACATGAGGACAGACCGCAGGCTGCAGAG
ACTCCTTCAGACCCAGAGGGAGCTGATGTCCAAGGAGCTCTGGCTGTACATCGGCATCAA
CACCTTTGACTATCTGGGCAGCATCCTGAGTTACGTTGTCATCGCAATCCCCATTTTCAG
CGGGGTCTATGGAGACCTGAGTCCCACAGAGCTTAGCACCCTGGTCAGCAAGAATGCCTT
TGTGTGCATCTACCTCATCAGCTGCTTCACCCAGCTAATCGACCTGTCCACGACGCTCTC
AGATGTGGCTGGCTACACGCACAGAATTGGGCAGCTTCGGGAGACGCTTCTGGACATGTC
CCTGAAGTCACAGGACTGCGAGATCCTGGGCGAGAGCAAGTGGGGCTTGGACACACCCCC
AGGGTGGCCAGCGGCAGAGCCAGCAGACACAGCATTTCTCCTTGAGCGGGTCTCCATCTC
TGCCCCCTCCTCTGACAAACCCCTAATCAAGGATCTGAGCCTAAAGATCTCCGAGGGACA
GAGCCTGCTCATCACAGGCAACACGGGCACTGGCAAGACCTCCTTGCTCCGGGTTCTGGG
TGGCCTCTGGACGAGTACACGGGGCTCAGTGCAGATGCTGACGGACTTTGGGCCCCATGG
GGTGCTATTCCTGCCACAAAAGCCATTCTTCACTGACGGGACCCTTCGGGAGCAGGTGAT
ATATCCCCTGAAGGAGGTCTACCCCGACTCAGGTTCTGCCGATGATGAGAGGATCTTGAG
GTTCTTGGAATTGGCAGGCCTGTCCAACTTGGTGGCAAGGACAGAGGGCCTGGACCAGCA
GGTGGACTGGAACTGGTATGATGTTCTGTCCCCGGGGGAGATGCAACGGCTCTCCTTTGC
CCGACTCTTCTACCTGCAGCCGAAGTACGCAGTGCTTGATGAAGCCACCAGTGCCCTGAC
AGAGGAAGTGGAGAGCGAGCTCTATCGCATCGGCCAGCAGCTGGGGATGACGTTCATCAG
TGTGGGACATCGGCAGAGCCTTGAGAAGTTTCATTCCTTGGTTCTGAAACTCTGTGGAGG
AGGAAGATGGGAGCTGATGAGAATCAAAGTGGAAGGTACCAGAGGTTCTCACCACCATCA
CCACCATCACCACCATCACTAATAA

Protein sequence predicted based on nucleotide sequence

AVAGPAPGAGARPRLDLQFLQRFLQILKVLFPSWSSQNALMFLTLLCLTLLEQFVIYQVG
LIPSQYYGVLGNKDLEGFKTLTFLAVMLIVLNSTLKSFDQFTCNLLYVSWRKDLTEHLHR
LYFRGRAYYTLNVLRDDIDNPDQRISQDVERFCRQLSSMASKLIISPFTLVYYTYQCFQS
TGWLGPVSIFGYFILGTVVNKTLMGPIVMKLVHQEKLEGDFRFKHMQIRVNAEPAAFYRA
GHVEHMRTDRRLQRLLQTQRELMSKELWLYIGINTFDYLGSILSYVVIAIPIFSGVYGDL
SPTELSTLVSKNAFVCIYLISCFTQLIDLSTTLSDVAGYTHRIGQLRETLLDMSLKSQDC
EILGESKWGLDTPPGWPAAEPADTAFLLERVSISAPSSDKPLIKDLSLKISEGQSLLITG
NTGTGKTSLLRVLGGLWTSTRGSVQMLTDFGPHGVLFLPQKPFFTDGTLREQVIYPLKEV
YPDSGSADDERILRFLELAGLSNLVARTEGLDQQVDWNWYDVLSPGEMQRLSFARLFYLQ
PKYAVLDEATSALTEEVESELYRIGQQLGMTFISVGHRQSLEKFHSLVLKLCGGGRWELM
RIKVE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: Pichia Marker: zeocin
Host Type: bacterial Marker: zeocin

Bacterial Selection Condition: 50 ug/ml zeocin

Growth Condition: Growth in low salt LB with 50ug/ml zeocin at 37 degrees C

Comments: None

Protein Expression Results:

SolubleProtein: Not_Applicable

ProteinExpressed: Protin_Confirmed

ProteinPurified: Tested_Not_Purified

Find experimental protocols in TransportPDB-0094

Recommended expression in:

Not Applicable

Vector Name:

pPICZa

Synonyms:

Sequencing Primer:

Forward: AOX1

Reverse: pAOX1 Reverse

Description:

with tag/fusion/marker assay, multiple cloning site clone, expression in Pichia pastoris, yeast expression expression with AOX1 and MCS and c-myc and His and TEF1 and EM7 and CYC1 and pUC ori and ZeoR; zeocin resistance in bacterial, zeocin resistance in Pichia;

Comments:

None

Size (bp):

3329

Parent Vector:

None

Properties:

expression in Pichia pastoris, multiple cloning site, with tag/fusion/marker, yeast expression

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAAACGAGGAATTCACGTGGCCCAGCCGGCCGTCTCGGATCGGTACCTCGAGCCGCGGCGGCCGCCAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTTAGCCTTAGACATGACTGTTCCTCAGTTCAAGTTGGGCACTTACGAGAAGACCGGTCTTGCTAGATTCTAATCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGACCTTCGTTTGTGCGGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAATTTTTTTTTTTAGTTTTTTTCTCTTTCAGTGACCTCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGTTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGGGGCGGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACACGTCCGACGGCGGCCCACGGGTCCCAGGCCTCGGAGATCCGTCCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGGAGACCAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATC

Vector Features:

Type

Name

Description

Start

End

MCS

multiple cloning site

932

1011

bacterial origin

pUC ori

pUC origin

2602

3275

promoter

AOX1

AOX1 promoter

941

promoter

TEF1

fragment containing TEF1 promoter

1419

1830

promoter

EM7

EM7 promoter

1831

1898

selectable marker

ZeoR

Zeocin resistance

1899

2273

tag

c-myc

N-terminal C-myc tag

1012

1044

tag

His

C-terminal 6xHis tag

1057

1077

trxn termination sequence

CYC1

CYC1 transcription termination region

2274

2591

Gene

Gene Symbol :

ABCD4

Symbol Nomenclature :

ABCD4

SYNONYM :

ABC41; EST352188; MAHCJ; P70R; P79R; PMP69; PXMP1L

Designation :

69 kDa peroxisomal ABC-transporter|ATP-binding cassette sub-family D member 4|PMP70-related protein|PXMP1-L|peroxisomal membrane protein 69

Full Nomenclature :

ATP-binding cassette, sub-family D (ALD), member 4

GENEID :

5826

GI :

4826958

GenBank Accession :

NM_005050

HGNC :

MIM :

603214

Vega :

OTTHUMG00000171207

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583074      CGATGACGATAAGTCTAGAGCGGTCGCGGGGCCCGCGCCCGGAGCTGGCGCCAGGCCCAG
NM_005050.3       ----------------ATGGCGGTCGCGGGGCCCGCGCCCGGAGCTGGCGCCAGGCCCAG
                                  * .*****************************************

HsCD00583074      GTTAGATCTGCAATTTCTCCAGCGGTTCCTGCAGATACTGAAGGTTTTGTTTCCTTCTTG
NM_005050.3       GTTAGATCTGCAATTTCTCCAGCGGTTCCTGCAGATACTGAAGGTTTTGTTTCCTTCTTG
                  ************************************************************

HsCD00583074      GTCATCACAAAATGCCTTGATGTTCCTGACCCTTTTGTGCCTGACCCTACTGGAGCAATT
NM_005050.3       GTCATCACAAAATGCCTTGATGTTCCTGACCCTTTTGTGCCTGACCCTACTGGAGCAATT
                  ************************************************************

HsCD00583074      TGTGATCTACCAGGTTGGCCTGATCCCCAGTCAGTACTATGGGGTCCTGGGAAACAAAGA
NM_005050.3       TGTGATCTACCAGGTTGGCTTGATCCCCAGTCAGTACTATGGGGTCCTGGGAAACAAAGA
                  ******************* ****************************************

HsCD00583074      CTTGGAAGGGTTTAAGACTCTGACATTCCTGGCTGTCATGCTCATTGTTCTGAACTCCAC
NM_005050.3       CTTGGAAGGGTTTAAGACTCTGACATTCCTGGCTGTCATGCTCATTGTTCTGAACTCCAC
                  ************************************************************

HsCD00583074      GCTGAAGAGCTTTGATCAGTTCACCTGCAACCTGCTGTATGTGAGCTGGAGGAAGGACCT
NM_005050.3       GCTGAAGAGCTTTGATCAGTTCACCTGCAACCTGCTGTATGTGAGCTGGAGGAAGGACCT
                  ************************************************************

HsCD00583074      CACTGAGCACCTTCACCGCCTCTACTTCCGGGGCCGTGCGTACTACACCCTCAACGTGCT
NM_005050.3       CACTGAGCACCTTCACCGCCTCTACTTCCGGGGCCGTGCGTACTACACCCTCAACGTGCT
                  ************************************************************

HsCD00583074      GCGGGATGACATCGATAACCCGGACCAGCGCATCAGCCAGGACGTGGAGCGATTCTGCCG
NM_005050.3       GCGGGATGACATCGATAACCCGGACCAGCGCATCAGCCAGGACGTGGAGCGATTCTGCCG
                  ************************************************************

HsCD00583074      GCAGCTCAGCAGCATGGCCAGCAAGCTCATCATCTCCCCGTTCACCCTCGTCTACTACAC
NM_005050.3       GCAGCTCAGCAGCATGGCCAGCAAGCTCATCATCTCCCCGTTCACCCTCGTCTACTACAC
                  ************************************************************

HsCD00583074      TTACCAGTGCTTCCAAAGCACAGGCTGGCTCGGGCCTGTGAGCATCTTCGGGTATTTCAT
NM_005050.3       TTACCAGTGCTTCCAAAGCACAGGCTGGCTCGGGCCTGTGAGCATCTTCGGGTATTTCAT
                  ************************************************************

HsCD00583074      CCTGGGGACCGTGGTGAACAAAACTTTGATGGGCCCCATTGTGATGAAGCTGGTGCATCA
NM_005050.3       CCTGGGGACCGTGGTGAACAAAACTTTGATGGGCCCCATTGTGATGAAGCTGGTGCATCA
                  ************************************************************

HsCD00583074      GGAGAAGCTGGAGGGAGATTTTAGGTTCAAGCACATGCAGATTCGGGTGAATGCGGAGCC
NM_005050.3       GGAGAAGCTGGAGGGAGATTTTAGGTTCAAGCACATGCAGATTCGGGTGAATGCGGAGCC
                  ************************************************************

HsCD00583074      TGCTGCTTTCTACAGAGCTGGGCATGTGGAGCACATGAGGACAGACCGCAGGCTGCAGAG
NM_005050.3       TGCTGCTTTCTACAGAGCTGGGCATGTGGAGCACATGAGGACAGACCGCAGGCTGCAGAG
                  ************************************************************

HsCD00583074      ACTCCTTCAGACCCAGAGGGAGCTGATGTCCAAGGAGCTCTGGCTGTACATCGGCATCAA
NM_005050.3       ACTCCTTCAGACCCAGAGGGAGCTGATGTCCAAGGAGCTCTGGCTGTACATCGGCATCAA
                  ************************************************************

HsCD00583074      CACCTTTGACTATCTGGGCAGCATCCTGAGTTACGTTGTCATCGCAATCCCCATTTTCAG
NM_005050.3       CACCTTTGACTATCTGGGCAGCATCCTGAGTTACGTTGTCATCGCAATCCCCATTTTCAG
                  ************************************************************

HsCD00583074      CGGGGTCTATGGAGACCTGAGTCCCACAGAGCTTAGCACCCTGGTCAGCAAGAATGCCTT
NM_005050.3       CGGGGTCTATGGAGACCTGAGTCCCGCAGAGCTTAGCACCCTGGTCAGCAAGAATGCCTT
                  *************************.**********************************

HsCD00583074      TGTGTGCATCTACCTCATCAGCTGCTTCACCCAGCTAATCGACCTGTCCACGACGCTCTC
NM_005050.3       TGTGTGCATCTACCTCATCAGCTGCTTCACCCAGCTCATCGACCTGTCCACGACGCTCTC
                  ************************************.***********************

HsCD00583074      AGATGTGGCTGGCTACACGCACAGAATTGGGCAGCTTCGGGAGACGCTTCTGGACATGTC
NM_005050.3       AGATGTGGCTGGCTACACGCACAGAATTGGGCAGCTTCGGGAGACGCTTCTGGACATGTC
                  ************************************************************

HsCD00583074      CCTGAAGTCACAGGACTGCGAGATCCTGGGCGAGAGCAAGTGGGGCTTGGACACACCCCC
NM_005050.3       CCTGAAGTCACAGGACTGCGAGATCCTGGGCGAGAGCGAGTGGGGCTTGGACACACCCCC
                  *************************************.**********************

HsCD00583074      AGGGTGGCCAGCGGCAGAGCCAGCAGACACAGCATTTCTCCTTGAGCGGGTCTCCATCTC
NM_005050.3       AGGGTGGCCAGCGGCAGAGCCAGCAGACACAGCATTTCTCCTTGAGCGGGTCTCCATCTC
                  ************************************************************

HsCD00583074      TGCCCCCTCCTCTGACAAACCCCTAATCAAGGATCTGAGCCTAAAGATCTCCGAGGGACA
NM_005050.3       TGCCCCCTCCTCTGACAAACCCCTAATCAAGGATCTGAGCCTAAAGATCTCCGAGGGACA
                  ************************************************************

HsCD00583074      GAGCCTGCTCATCACAGGCAACACGGGCACTGGCAAGACCTCCTTGCTCCGGGTTCTGGG
NM_005050.3       GAGCCTGCTCATCACAGGCAACACGGGCACTGGCAAGACCTCCTTGCTCCGGGTTCTGGG
                  ************************************************************

HsCD00583074      TGGCCTCTGGACGAGTACACGGGGCTCAGTGCAGATGCTGACGGACTTTGGGCCCCATGG
NM_005050.3       TGGCCTCTGGACGAGTACACGGGGCTCAGTGCAGATGCTGACGGACTTTGGGCCCCATGG
                  ************************************************************

HsCD00583074      GGTGCTATTCCTGCCACAAAAGCCATTCTTCACTGACGGGACCCTTCGGGAGCAGGTGAT
NM_005050.3       GGTGCTATTCCTGCCACAAAAGCCATTCTTCACTGACGGGACCCTTCGGGAGCAGGTGAT
                  ************************************************************

HsCD00583074      ATATCCCCTGAAGGAGGTCTACCCCGACTCAGGTTCTGCCGATGATGAGAGGATCTTGAG
NM_005050.3       ATATCCCCTGAAGGAGGTCTACCCCGACTCAGGTTCTGCCGATGATGAGAGGATCTTGAG
                  ************************************************************

HsCD00583074      GTTCTTGGAATTGGCAGGCCTGTCCAACTTGGTGGCAAGGACAGAGGGCCTGGACCAGCA
NM_005050.3       GTTCTTGGAATTGGCAGGCCTGTCCAACTTGGTGGCAAGGACAGAGGGCCTGGACCAGCA
                  ************************************************************

HsCD00583074      GGTGGACTGGAACTGGTATGATGTTCTGTCCCCGGGGGAGATGCAACGGCTCTCCTTTGC
NM_005050.3       GGTGGACTGGAACTGGTATGATGTTCTGTCCCCGGGGGAGATGCAACGGCTCTCCTTTGC
                  ************************************************************

HsCD00583074      CCGACTCTTCTACCTGCAGCCGAAGTACGCAGTGCTTGATGAAGCCACCAGTGCCCTGAC
NM_005050.3       CCGACTCTTCTACCTGCAGCCGAAGTACGCAGTGCTTGATGAAGCCACCAGTGCCCTGAC
                  ************************************************************

HsCD00583074      AGAGGAAGTGGAGAGCGAGCTCTATCGCATCGGCCAGCAGCTGGGGATGACGTTCATCAG
NM_005050.3       AGAGGAAGTGGAGAGCGAGCTCTATCGCATCGGCCAGCAGCTGGGGATGACGTTCATCAG
                  ************************************************************

HsCD00583074      TGTGGGACATCGGCAGAGCCTTGAGAAGTTTCATTCCTTGGTTCTGAAACTCTGTGGAGG
NM_005050.3       TGTGGGACATCGGCAGAGCCTTGAGAAGTTTCATTCCTTGGTTCTGAAACTCTGTGGAGG
                  ************************************************************

HsCD00583074      AGGAAGATGGGAGCTGATGAGAATCAAAGTGGAAGGTACCAGAGGTTCTCACCACCATCA
NM_005050.3       AGGAAGATGGGAGCTGATGAGAATCAAAGTGGAATGA-----------------------
                  ********************************** *:                       

HsCD00583074      CCACCATCACCACCATCACTAATAA
NM_005050.3       -------------------------