DNASU Plasmid | HHATL (Homo sapiens) in pPICZa

Explanation of Terms

Gene:

Gene Symbol: HHATL

Gene Name: hedgehog acyltransferase-like

Original Clone ID:

TPDB-0164

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pPICZa

Format:

FUSION

Source:

Center for the X-ray Structure Determination of Human Transporters

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Center for the X-ray Structure Determination of Human Transporters

HsCD00583110

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1509nts

Open reading frame: 19 to 1527

Reference Sequence Alignment

GATGACGATAAGTCTAGAGGCATCAAGACAGCATTGCCGGCGGCTGAGCTGGGCCTCTAC
TCTCTGGTGCTGAGTGGGGCCCTGGCCTATGCTGGCCGGGGCCTCCTTGAGGCTTCACAA
GATGGGGCCCACAGGAAGGCCTTCCGGGAGTCTGTGCGACCTGGCTGGGAGTACATTGGC
CGGAAGATGGATGTGGCTGACTTCGAGTGGGTGATGTGGTTCACCTCCTTTCGCAACGTC
ATCATCTTTGCCCTCTCCGGACATGTGCTGTTTGCTAAACTCTGCACGATGGTTGCCCCA
AAGCTCCGCTCCTGGATGTATGCTGTGTACGGGGCCTTGGCTGTGATGGGCACAATGGGC
CCTTGGTACCTGCTGCTGCTGCTTGGTCACTGTGTGGGCCTCTATGTGGCCTCGCTTTTG
GGCCAGCCCTGGCTCTGTCTTGGCCTTGGCTTGGCCAGCCTGGCCTCCTTCAAGATGGAC
CCCCTAATCTCTTGGCAGAGCGGGTTTGTAACAGGCACTTTTGATCTTCAAGAGGTGCTG
TTTCATGGGGGCAGCAGCTTCACAGTGCTGCGTTGCGCCAGCTTTGCACTGGAGAGCTGT
GCCCACCCTGACCGCCACTACTCCTTAGCTGACCTGCTCAAGTACAACTTCTACCTGCCC
TTCTTCTTCTTCGGGCCAATCATGACCTTTGATCGCTTCCATGCTCAGGTGAGCCAGGTG
GAGCCAGTGAGACGCGAGGGTGAGCTGTGGCACATCCGAGCCCAGGCAGGCCTAAGCGTG
GTGGCCATCATGGCCGTCGACATCTTCTTTCACTTCTTCTACATCCTCACTATCCCCAGC
GACCTCAAGTTCGCCAACCGCCTCCCAGACAGTGCCCTCGCTGGCCTAGCCTATTCAAAC
CTGGTGTATGACTGGGTGAAGGCGGCCGTCCTCTTTGGTGTTGTCAACACTGTGGCATGC
CTCGACCACCTGGACCCACCCCAGCCTCCCAAGTGCATCACCGCACTCTACGTCTTTACG
GAAACGCACTTTGACCGTGGCATCAACGACTGGCTTTGCAAATATGTGTATAACCACATT
GGTGGGGAGCATTCCGCTGTGATCCCAGAGCTGGCAGCCACAGTGGCCACATTTGCCATC
ACCACACTGTGGCTTGGGCCTTGTGACATTGTCTACCTGTGGTCATTCCTTAACTGCTTT
GGCCTCAACTTTGAGCTCTGGATGCAAAAACTGGCAGAGTGGGGGCCCCTAGCACGAATT
GAGGCCTCTCTGTCAGTGCAGATGTCCCGTAGGGTCCGGGCCCTGTTTGGAGCCATGAAC
TTCTGGGCCATCATCATGTACAACCTTGTGAGCCTGAACAGCCTCAAATTCACAGAGCTG
GTTGCCCGGCGCCTGCTACTCACAGGGTTTCCCCAGACCACGCTGTCCATCCTGTTTGTC
ACCTACTGTGGCGTCCAGCTGGTAAAGGAGCGTGAGCGAACCTTGGCACTGGAGGAGGAG
CAGAAGCAGGACAAAGAGAAGCCGGAGGGTACCAGAGGTTCTCACCACCATCACCACCAT
CACCACCATCACTAATAA

Protein sequence predicted based on nucleotide sequence

GIKTALPAAELGLYSLVLSGALAYAGRGLLEASQDGAHRKAFRESVRPGWEYIGRKMDVA
DFEWVMWFTSFRNVIIFALSGHVLFAKLCTMVAPKLRSWMYAVYGALAVMGTMGPWYLLL
LLGHCVGLYVASLLGQPWLCLGLGLASLASFKMDPLISWQSGFVTGTFDLQEVLFHGGSS
FTVLRCASFALESCAHPDRHYSLADLLKYNFYLPFFFFGPIMTFDRFHAQVSQVEPVRRE
GELWHIRAQAGLSVVAIMAVDIFFHFFYILTIPSDLKFANRLPDSALAGLAYSNLVYDWV
KAAVLFGVVNTVACLDHLDPPQPPKCITALYVFTETHFDRGINDWLCKYVYNHIGGEHSA
VIPELAATVATFAITTLWLGPCDIVYLWSFLNCFGLNFELWMQKLAEWGPLARIEASLSV
QMSRRVRALFGAMNFWAIIMYNLVSLNSLKFTELVARRLLLTGFPQTTLSILFVTYCGVQ
LVKERERTLALEEEQKQDKEKPE

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: Pichia Marker: zeocin
Host Type: bacterial Marker: zeocin

Bacterial Selection Condition: 50 ug/ml zeocin

Growth Condition: Growth in low salt LB with 50ug/ml zeocin at 37 degrees C

Comments: None

Protein Expression Results:

SolubleProtein: Not_Applicable

ProteinPurified: Protein_Purified

ProteinExpressed: Protin_Confirmed

Find experimental protocols in TransportPDB-0164

Recommended expression in:

Not Applicable

Vector Name:

pPICZa

Synonyms:

Sequencing Primer:

Forward: AOX1

Reverse: pAOX1 Reverse

Description:

with tag/fusion/marker assay, multiple cloning site clone, expression in Pichia pastoris, yeast expression expression with AOX1 and MCS and c-myc and His and TEF1 and EM7 and CYC1 and pUC ori and ZeoR; zeocin resistance in bacterial, zeocin resistance in Pichia;

Comments:

None

Size (bp):

3329

Parent Vector:

None

Properties:

expression in Pichia pastoris, multiple cloning site, with tag/fusion/marker, yeast expression

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAAACGAGGAATTCACGTGGCCCAGCCGGCCGTCTCGGATCGGTACCTCGAGCCGCGGCGGCCGCCAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTTAGCCTTAGACATGACTGTTCCTCAGTTCAAGTTGGGCACTTACGAGAAGACCGGTCTTGCTAGATTCTAATCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGACCTTCGTTTGTGCGGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAATTTTTTTTTTTAGTTTTTTTCTCTTTCAGTGACCTCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGTTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGGGGCGGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACACGTCCGACGGCGGCCCACGGGTCCCAGGCCTCGGAGATCCGTCCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGGAGACCAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATC

Vector Features:

Type

Name

Description

Start

End

MCS

multiple cloning site

932

1011

bacterial origin

pUC ori

pUC origin

2602

3275

promoter

AOX1

AOX1 promoter

941

promoter

TEF1

fragment containing TEF1 promoter

1419

1830

promoter

EM7

EM7 promoter

1831

1898

selectable marker

ZeoR

Zeocin resistance

1899

2273

tag

c-myc

N-terminal C-myc tag

1012

1044

tag

His

C-terminal 6xHis tag

1057

1077

trxn termination sequence

CYC1

CYC1 transcription termination region

2274

2591

Gene

Gene Symbol :

HHATL

Symbol Nomenclature :

HHATL

SYNONYM :

C3orf3; GUP1; MBOAT3; MSTP002; OACT3

Designation :

GUP1 glycerol uptake/transporter homolog|glycerol uptake/transporter homolog|hedgehog acyltransferase-like protein|membrane bound O-acyltransferase domain containing 3|protein-cysteine N-palmitoyltransferase HHAT-like protein

Full Nomenclature :

hedgehog acyltransferase-like

GENEID :

57467

GI :

239582769

GenBank Accession :

NM_020707

HGNC :

13242

MIM :

608116

Vega :

OTTHUMG00000133043

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583110      GATGACGATAAGTCTAGAGGCATCAAGACAGCATTGCCGGCGGCTGAGCTGGGCCTCTAC
NM_020707.3       ---------------ATGGGCATCAAGACAGCATTGCCGGCGGCTGAGCTGGGCCTCTAC
                                 * .******************************************

HsCD00583110      TCTCTGGTGCTGAGTGGGGCCCTGGCCTATGCTGGCCGGGGCCTCCTTGAGGCTTCACAA
NM_020707.3       TCTCTGGTGCTGAGTGGGGCCCTGGCCTATGCTGGCCGGGGCCTCCTTGAGGCTTCACAA
                  ************************************************************

HsCD00583110      GATGGGGCCCACAGGAAGGCCTTCCGGGAGTCTGTGCGACCTGGCTGGGAGTACATTGGC
NM_020707.3       GATGGGGCCCACAGGAAGGCCTTCCGGGAGTCTGTGCGACCTGGCTGGGAGTACATTGGC
                  ************************************************************

HsCD00583110      CGGAAGATGGATGTGGCTGACTTCGAGTGGGTGATGTGGTTCACCTCCTTTCGCAACGTC
NM_020707.3       CGGAAGATGGATGTGGCTGACTTCGAGTGGGTGATGTGGTTCACCTCCTTTCGCAACGTC
                  ************************************************************

HsCD00583110      ATCATCTTTGCCCTCTCCGGACATGTGCTGTTTGCTAAACTCTGCACGATGGTTGCCCCA
NM_020707.3       ATCATCTTTGCCCTCTCCGGACATGTGCTGTTTGCTAAACTCTGCACGATGGTTGCCCCA
                  ************************************************************

HsCD00583110      AAGCTCCGCTCCTGGATGTATGCTGTGTACGGGGCCTTGGCTGTGATGGGCACAATGGGC
NM_020707.3       AAGCTCCGCTCCTGGATGTATGCTGTGTACGGGGCCTTGGCTGTGATGGGCACAATGGGC
                  ************************************************************

HsCD00583110      CCTTGGTACCTGCTGCTGCTGCTTGGTCACTGTGTGGGCCTCTATGTGGCCTCGCTTTTG
NM_020707.3       CCTTGGTACCTGCTGCTGCTGCTTGGTCACTGTGTGGGCCTCTATGTGGCCTCGCTTTTG
                  ************************************************************

HsCD00583110      GGCCAGCCCTGGCTCTGTCTTGGCCTTGGCTTGGCCAGCCTGGCCTCCTTCAAGATGGAC
NM_020707.3       GGCCAGCCCTGGCTCTGTCTTGGCCTTGGCTTGGCCAGCCTGGCCTCCTTCAAGATGGAC
                  ************************************************************

HsCD00583110      CCCCTAATCTCTTGGCAGAGCGGGTTTGTAACAGGCACTTTTGATCTTCAAGAGGTGCTG
NM_020707.3       CCCCTAATCTCTTGGCAGAGCGGGTTTGTAACAGGCACTTTTGATCTTCAAGAGGTGCTG
                  ************************************************************

HsCD00583110      TTTCATGGGGGCAGCAGCTTCACAGTGCTGCGTTGCGCCAGCTTTGCACTGGAGAGCTGT
NM_020707.3       TTTCATGGGGGCAGCAGCTTCACAGTGCTGCGTTGCACCAGCTTTGCACTGGAGAGCTGT
                  ************************************.***********************

HsCD00583110      GCCCACCCTGACCGCCACTACTCCTTAGCTGACCTGCTCAAGTACAACTTCTACCTGCCC
NM_020707.3       GCCCACCCTGACCGCCACTACTCCTTAGCTGACCTGCTCAAGTACAACTTCTACCTGCCC
                  ************************************************************

HsCD00583110      TTCTTCTTCTTCGGGCCAATCATGACCTTTGATCGCTTCCATGCTCAGGTGAGCCAGGTG
NM_020707.3       TTCTTCTTCTTCGGGCCCATCATGACCTTTGATCGCTTCCATGCTCAGGTGAGCCAGGTG
                  *****************.******************************************

HsCD00583110      GAGCCAGTGAGACGCGAGGGTGAGCTGTGGCACATCCGAGCCCAGGCAGGCCTAAGCGTG
NM_020707.3       GAGCCAGTGAGACGCGAGGGTGAGCTGTGGCACATCCGAGCCCAGGCAGGCCTAAGCGTG
                  ************************************************************

HsCD00583110      GTGGCCATCATGGCCGTCGACATCTTCTTTCACTTCTTCTACATCCTCACTATCCCCAGC
NM_020707.3       GTGGCCATCATGGCCGTCGACATCTTCTTTCACTTCTTCTACATCCTCACTATCCCCAGC
                  ************************************************************

HsCD00583110      GACCTCAAGTTCGCCAACCGCCTCCCAGACAGTGCCCTCGCTGGCCTAGCCTATTCAAAC
NM_020707.3       GACCTCAAGTTCGCCAACCGCCTCCCAGACAGTGCCCTCGCTGGCCTAGCCTATTCAAAC
                  ************************************************************

HsCD00583110      CTGGTGTATGACTGGGTGAAGGCGGCCGTCCTCTTTGGTGTTGTCAACACTGTGGCATGC
NM_020707.3       CTGGTGTATGACTGGGTGAAGGCGGCCGTCCTCTTTGGTGTTGTCAACACTGTGGCATGC
                  ************************************************************

HsCD00583110      CTCGACCACCTGGACCCACCCCAGCCTCCCAAGTGCATCACCGCACTCTACGTCTTTACG
NM_020707.3       CTCGACCACCTGGACCCACCCCAGCCTCCCAAGTGCATCACCGCACTCTACGTCTTTGCG
                  *********************************************************.**

HsCD00583110      GAAACGCACTTTGACCGTGGCATCAACGACTGGCTTTGCAAATATGTGTATAACCACATT
NM_020707.3       GAAACGCACTTTGACCGTGGCATCAACGACTGGCTTTGCAAATATGTGTATAACCACATT
                  ************************************************************

HsCD00583110      GGTGGGGAGCATTCCGCTGTGATCCCAGAGCTGGCAGCCACAGTGGCCACATTTGCCATC
NM_020707.3       GGTGGGGAGCATTCCGCTGTGATCCCAGAGCTGGCAGCCACAGTGGCCACATTTGCCATC
                  ************************************************************

HsCD00583110      ACCACACTGTGGCTTGGGCCTTGTGACATTGTCTACCTGTGGTCATTCCTTAACTGCTTT
NM_020707.3       ACCACACTGTGGCTTGGGCCTTGTGACATTGTCTACCTGTGGTCATTCCTTAACTGCTTT
                  ************************************************************

HsCD00583110      GGCCTCAACTTTGAGCTCTGGATGCAAAAACTGGCAGAGTGGGGGCCCCTAGCACGAATT
NM_020707.3       GGCCTCAACTTTGAGCTCTGGATGCAAAAACTGGCAGAGTGGGGGCCCCTAGCACGAATT
                  ************************************************************

HsCD00583110      GAGGCCTCTCTGTCAGTGCAGATGTCCCGTAGGGTCCGGGCCCTGTTTGGAGCCATGAAC
NM_020707.3       GAGGCCTCTCTGTCAGTGCAGATGTCCCGTAGGGTCCGGGCCCTGTTTGGAGCCATGAAC
                  ************************************************************

HsCD00583110      TTCTGGGCCATCATCATGTACAACCTTGTGAGCCTGAACAGCCTCAAATTCACAGAGCTG
NM_020707.3       TTCTGGGCCATCATCATGTACAACCTTGTGAGCCTGAACAGCCTCAAATTCACAGAGCTG
                  ************************************************************

HsCD00583110      GTTGCCCGGCGCCTGCTACTCACAGGGTTTCCCCAGACCACGCTGTCCATCCTGTTTGTC
NM_020707.3       GTTGCCCGGCGCCTGCTACTCACAGGGTTCCCCCAGACCACGCTGTCCATCCTGTTTGTC
                  ***************************** ******************************

HsCD00583110      ACCTACTGTGGCGTCCAGCTGGTAAAGGAGCGTGAGCGAACCTTGGCACTGGAGGAGGAG
NM_020707.3       ACCTACTGTGGCGTCCAGCTGGTAAAGGAGCGTGAGCGAACCTTGGCACTGGAGGAGGAG
                  ************************************************************

HsCD00583110      CAGAAGCAGGACAAAGAGAAGCCGGAGGGTACCAGAGGTTCTCACCACCATCACCACCAT
NM_020707.3       CAGAAGCAGGACAAAGAGAAGCCGGAGTAG------------------------------
                  *************************** .                               

HsCD00583110      CACCACCATCACTAATAA
NM_020707.3       ------------------