DNASU Plasmid | SLC18A3 (Homo sapiens) in pPICZa

Explanation of Terms

Gene:

Gene Symbol: SLC18A3

Gene Name: solute carrier family 18 (vesicular acetylcholine transporter), member 3

Original Clone ID:

TPDB-0034

Keyword:

None

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pPICZa

Format:

FUSION

Source:

Center for the X-ray Structure Determination of Human Transporters

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

Geoffrey Chang
Chang-Wook Lee
PSI:Biology
Center for the X-ray Structure Determination of Human Transporters

HsCD00583116

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 1593nts

Open reading frame: 51 to 1643

Reference Sequence Alignment

GAGGAATTCAAAAACCATGCTACTACAAGGACGATGACGATAAGTCTAGAGAGTCCGCCG
AACCTGCAGGACAGGCTAGAGCTGCCGCAACTAAGCTGTCTGAGGCTGTTGGTGCTGCCT
TGCAGGAACCAAGAAGACAGAGAAGATTGGTCTTGGTGATCGTTTGTGTCGCCTTGTTGT
TGGACAACATGCTGTATATGGTGATCGTTCCAATTGTTCCTGATTACATTGCTCACATGA
GAGGTGGAGGCGAGGGTCCTACAAGAACTCCAGAGGTCTGGGAACCTACACTGCCACTTC
CTACCCCCGCTAACGCCTCCGCATATACCGCTAATACATCAGCCAGTCCTACTGCAGCTT
GGCCAGCCGGATCCGCATTGCGTCCCAGATACCCAACCGAGTCTGAAGACGTGAAGATCG
GTGTTTTGTTTGCTTCCAAAGCCATTCTGCAGTTACTCGTTAATCCTTTGTCCGGTCCAT
TCATCGACAGAATGTCTTATGATGTCCCATTGCTGATTGGCCTGGGTGTTATGTTCGCCT
CTACTGTCTTGTTCGCTTTTGCCGAGGATTACGCTACCCTGTTTGCCGCAAGATCATTAC
AAGGATTGGGCAGTGCATTCGCTGACACTAGCGGTATCGCAATGATTGCCGATAAGTACC
CTGAGGAACCAGAGAGATCCAGAGCTTTGGGAGTTGCCCTGGCATTCATCAGTTTTGGTA
GCCTGGTCGCCCCACCTTTCGGTGGAATTTTGTACGAATTTGCTGGTAAGAGAGTCCCTT
TCTTAGTTTTGGCTGCCGTCTCCTTGTTTGACGCCCTTTTACTCTTGGCTGTTGCCAAAC
CATTCTCTGCAGCTGCCAGAGCACGTGCTAACCTGCCTGTCGGAACCCCAATCCACAGAC
TGATGCTTGATCCATACATTGCTGTTGTCGCCGGTGCATTGACTACCTGTAATATCCCTC
TGGCCTTCTTGGAGCCAACTATTGCTACCTGGATGAAACATACTATGGCAGCTTCTGAGT
GGGAAATGGGAATGGCTTGGCTGCCTGCCTTCGTGCCACACGTTCTTGGTGTCTACTTGA
CCGTTAGACTGGCCGCAAGATATCCTCATTTGCAGTGGCTGTACGGAGCTTTAGGTTTGG
CCGTTATCGGTGCTTCTTCCTGTATTGTCCCTGCCTGCAGAAGTTTTGCTCCACTGGTGG
TTTCCCTTTGTGGATTGTGCTTCGGTATCGCCTTGGTTGACACTGCTCTGCTTCCAACCC
TGGCCTTTCTTGTCGATGTGAGACACGTTTCTGTCTATGGTTCCGTTTACGCTATCGCCG
ACATTAGCTATTCTGTCGCATACGCTTTGGGACCTATCGTTGCTGGTCACATTGTCCATT
CACTGGGTTTCGAGCAACTGTCTCTTGGAATGGGTTTGGCCAACTTACTCTACGCTCCAG
TTTTGCTGCTTTTAAGAAATGTCGGTCTCTTGACTAGATCCAGATCAGAGAGAGACGTTT
TGTTGGACGAACCCCCACAGGGTTTGTATGACGCCGTTAGACTGAGAGAGAGACCAGTCT
CCGGACAAGACGGTGAACCTAGATCTCCTCCCGGTCCATTCGATGCTTGCGAAGACGATT
ACAACTACTATTACACCAGATCTGGTACCAGAGGTTCTCACCACCATCACCACCATCACC
ACCATCACTAATAA

Protein sequence predicted based on nucleotide sequence

ESAEPAGQARAAATKLSEAVGAALQEPRRQRRLVLVIVCVALLLDNMLYMVIVPIVPDYI
AHMRGGGEGPTRTPEVWEPTLPLPTPANASAYTANTSASPTAAWPAGSALRPRYPTESED
VKIGVLFASKAILQLLVNPLSGPFIDRMSYDVPLLIGLGVMFASTVLFAFAEDYATLFAA
RSLQGLGSAFADTSGIAMIADKYPEEPERSRALGVALAFISFGSLVAPPFGGILYEFAGK
RVPFLVLAAVSLFDALLLLAVAKPFSAAARARANLPVGTPIHRLMLDPYIAVVAGALTTC
NIPLAFLEPTIATWMKHTMAASEWEMGMAWLPAFVPHVLGVYLTVRLAARYPHLQWLYGA
LGLAVIGASSCIVPACRSFAPLVVSLCGLCFGIALVDTALLPTLAFLVDVRHVSVYGSVY
AIADISYSVAYALGPIVAGHIVHSLGFEQLSLGMGLANLLYAPVLLLLRNVGLLTRSRSE
RDVLLDEPPQGLYDAVRLRERPVSGQDGEPRSPPGPFDACEDDYNYYYTRS

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: Pichia Marker: zeocin
Host Type: bacterial Marker: zeocin

Bacterial Selection Condition: 50 ug/ml zeocin

Growth Condition: Growth in low salt LB with 50ug/ml zeocin at 37 degrees C

Comments: None

Protein Expression Results:

SolubleProtein: Not_Applicable

ProteinExpressed: Protin_Confirmed

ProteinPurified: Tested_Not_Purified

Find experimental protocols in TransportPDB-0034

Recommended expression in:

Not Applicable

Vector Name:

pPICZa

Synonyms:

Sequencing Primer:

Forward: AOX1

Reverse: pAOX1 Reverse

Description:

with tag/fusion/marker assay, multiple cloning site clone, expression in Pichia pastoris, yeast expression expression with AOX1 and MCS and c-myc and His and TEF1 and EM7 and CYC1 and pUC ori and ZeoR; zeocin resistance in bacterial, zeocin resistance in Pichia;

Comments:

None

Size (bp):

3329

Parent Vector:

None

Properties:

expression in Pichia pastoris, multiple cloning site, with tag/fusion/marker, yeast expression

Author Name:

Invitrogen

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

AGATCTAACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGGCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTGACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAAACGAGGAATTCACGTGGCCCAGCCGGCCGTCTCGGATCGGTACCTCGAGCCGCGGCGGCCGCCAGCTTGGGCCCGAACAAAAACTCATCTCAGAAGAGGATCTGAATAGCGCCGTCGACCATCATCATCATCATCATTGAGTTTTAGCCTTAGACATGACTGTTCCTCAGTTCAAGTTGGGCACTTACGAGAAGACCGGTCTTGCTAGATTCTAATCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGACCTTCGTTTGTGCGGATCCCCCACACACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATACTAAATTTTCCCTCTTTCTTCCTCTAGGGTGTCGTTAATTACCCGTACTAAAGGTTTGGAAAAGAAAAAAGAGACCGCCTCGTTTCTTTTTCTTCGTCGAAAAAGGCAATAAAAATTTTTATCACGTTTCTTTTTCTTGAAATTTTTTTTTTTAGTTTTTTTCTCTTTCAGTGACCTCCATTGATATTTAAGTTAATAAACGGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGTTCATTAGAAAGAAAGCATAGCAATCTAATCTAAGGGGCGGTGTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACCATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGACACGTCCGACGGCGGCCCACGGGTCCCAGGCCTCGGAGATCCGTCCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAGCTGGAGACCAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCAATGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATC

Vector Features:

Type

Name

Description

Start

End

MCS

multiple cloning site

932

1011

bacterial origin

pUC ori

pUC origin

2602

3275

promoter

AOX1

AOX1 promoter

941

promoter

TEF1

fragment containing TEF1 promoter

1419

1830

promoter

EM7

EM7 promoter

1831

1898

selectable marker

ZeoR

Zeocin resistance

1899

2273

tag

c-myc

N-terminal C-myc tag

1012

1044

tag

His

C-terminal 6xHis tag

1057

1077

trxn termination sequence

CYC1

CYC1 transcription termination region

2274

2591

Gene

Gene Symbol :

SLC18A3

Symbol Nomenclature :

SLC18A3

SYNONYM :

VACHT

Designation :

solute carrier family 18 (vesicular acetylcholine), member 3|solute carrier family 18 member 3|solute carrier family 18, member 3|vesicular acetylcholine transporter

Full Nomenclature :

solute carrier family 18 (vesicular acetylcholine transporter), member 3

GENEID :

6572

GI :

118582257

GenBank Accession :

NM_003055

HGNC :

10936

MIM :

600336

Vega :

OTTHUMG00000018196

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00583116      GAGGAATTCAAAAACCATGCTACTACAAGGACGATGACGATAAGTCTAGAGAGTCCGCCG
NM_003055.2       -----------------------------------------------ATGGAATCCGCGG
                                                                 * .**.***** *

HsCD00583116      AACCTGCAGGACAGGCTAGAGCTGCCGCAACTAAGCTGTCTGAGGCTGTTGGTGCTGCCT
NM_003055.2       AACCTGCGGGCCAGGCCCGGGCGGCGGCCACCAAGCTGTCGGAGGCTGTGGGCGCGGCGC
                  *******.**.***** .*.** ** **.** ******** ******** ** ** **  

HsCD00583116      TGCAGGAACCAAGAAGACAGAGAAGATTGGTCTTGGTGATCGTTTGTGTCGCCTTGTTGT
NM_003055.2       TGCAGGAGCCCCGGCGGCAGAGGCGCCTGGTGCTTGTTATCGTGTGCGTGGCGCTGTTAC
                  *******.**..*..*.*****..*. ****  * ** ***** ** ** **  ****. 

HsCD00583116      TGGACAACATGCTGTATATGGTGATCGTTCCAATTGTTCCTGATTACATTGCTCACATGA
NM_003055.2       TGGACAACATGCTGTACATGGTCATCGTGCCCATAGTGCCCGACTACATCGCCCACATGC
                  **************** ***** ***** **.**:** ** ** ***** ** ******.

HsCD00583116      GAGGTGGAGGCGAGGGTCCTACAAGAACTCCAGAGGTCTGGGAACCTACACTGCCACTTC
NM_003055.2       GCGGGGGCGGCGAGGGCCCCACCCGGACTCCCGAGGTGTGGGAGCCCACCCTGCCGCTGC
                  *.** **.******** ** **..*.*****.***** *****.** **.*****.** *

HsCD00583116      CTACCCCCGCTAACGCCTCCGCATATACCGCTAATACATCAGCCAGTCCTACTGCAGCTT
NM_003055.2       CCACTCCGGCCAATGCCAGCGCCTACACGGCCAACACCTCGGCGTCCCCGACAGCTGCGT
                  * ** ** ** ** ***: ***.** ** ** ** **.**.** :  ** **:**:** *

HsCD00583116      GGCCAGCCGGATCCGCATTGCGTCCCAGATACCCAACCGAGTCTGAAGACGTGAAGATCG
NM_003055.2       GGCCAGCGGGCTCAGCCCTTCGGCCCCGCTACCCTACGGAGAGCGAAGACGTGAAGATCG
                  ******* **.**.**. * ** ***.*.*****:** ***:  ****************

HsCD00583116      GTGTTTTGTTTGCTTCCAAAGCCATTCTGCAGTTACTCGTTAATCCTTTGTCCGGTCCAT
NM_003055.2       GGGTGCTGTTTGCTTCCAAGGCTATCCTGCAGCTGCTAGTGAACCCCTTGAGCGGGCCCT
                  * **  *************.** ** ****** *.**.** ** ** ***: *** **.*

HsCD00583116      TCATCGACAGAATGTCTTATGATGTCCCATTGCTGATTGGCCTGGGTGTTATGTTCGCCT
NM_003055.2       TCATCGACCGCATGAGCTACGACGTGCCGCTGCTGATCGGCCTGGGCGTCATGTTCGCCT
                  ********.*.***:  ** ** ** **. ******* ******** ** **********

HsCD00583116      CTACTGTCTTGTTCGCTTTTGCCGAGGATTACGCTACCCTGTTTGCCGCAAGATCATTAC
NM_003055.2       CTACAGTCCTGTTCGCCTTCGCCGAGGACTACGCCACGCTGTTCGCGGCGCGCAGCCTGC
                  ****:*** ******* ** ******** ***** ** ***** ** **..*.: . *.*

HsCD00583116      AAGGATTGGGCAGTGCATTCGCTGACACTAGCGGTATCGCAATGATTGCCGATAAGTACC
NM_003055.2       AGGGCCTGGGCTCAGCCTTCGCCGACACGTCTGGCATAGCCATGATCGCCGATAAGTACC
                  *.**. *****: :**.***** ***** :  ** **.**.***** *************

HsCD00583116      CTGAGGAACCAGAGAGATCCAGAGCTTTGGGAGTTGCCCTGGCATTCATCAGTTTTGGTA
NM_003055.2       CGGAGGAGCCGGAGCGCAGTCGTGCACTGGGCGTGGCGCTGGCCTTCATTAGCTTCGGAA
                  * *****.**.***.*.:  .*:**: ****.** ** *****.***** ** ** **:*

HsCD00583116      GCCTGGTCGCCCCACCTTTCGGTGGAATTTTGTACGAATTTGCTGGTAAGAGAGTCCCTT
NM_003055.2       GCCTAGTGGCCCCGCCCTTCGGGGGCATCCTCTATGAGTTCGCCGGCAAGCGCGTGCCCT
                  ****.** *****.** ***** **.**  * ** **.** ** ** ***.*.** ** *

HsCD00583116      TCTTAGTTTTGGCTGCCGTCTCCTTGTTTGACGCCCTTTTACTCTTGGCTGTTGCCAAAC
NM_003055.2       TCTTGGTGCTAGCTGCCGTGTCGCTCTTTGACGCGCTGTTGCTGCTGGCAGTGGCCAAAC
                  ****.**  *.******** **  * ******** ** **.**  ****:** *******

HsCD00583116      CATTCTCTGCAGCTGCCAGAGCACGTGCTAACCTGCCTGTCGGAACCCCAATCCACAGAC
NM_003055.2       CCTTCTCGGCGGCTGCACGGGCTCGGGCCAACCTGCCAGTGGGCACTCCCATCCACCGCC
                  *.***** **.*****..*.**:** ** ********:** **.** **.******.*.*

HsCD00583116      TGATGCTTGATCCATACATTGCTGTTGTCGCCGGTGCATTGACTACCTGTAATATCCCTC
NM_003055.2       TCATGCTAGACCCCTACATTGCCGTGGTGGCCGGCGCGCTCACCACCTGTAACATTCCCC
                  * *****:** **.******** ** ** ***** **. * ** ******** ** ** *

HsCD00583116      TGGCCTTCTTGGAGCCAACTATTGCTACCTGGATGAAACATACTATGGCAGCTTCTGAGT
NM_003055.2       TCGCCTTCCTCGAACCCACCATTGCCACGTGGATGAAGCATACGATGGCGGCTTCCGAGT
                  * ****** * **.**.** ***** ** ********.***** *****.***** ****

HsCD00583116      GGGAAATGGGAATGGCTTGGCTGCCTGCCTTCGTGCCACACGTTCTTGGTGTCTACTTGA
NM_003055.2       GGGAGATGGGCATGGCCTGGCTGCCGGCCTTCGTGCCTCATGTGCTGGGCGTCTACCTCA
                  ****.*****.***** ******** ***********:** ** ** ** ****** * *

HsCD00583116      CCGTTAGACTGGCCGCAAGATATCCTCATTTGCAGTGGCTGTACGGAGCTTTAGGTTTGG
NM_003055.2       CCGTGCGCCTGGCGGCGCGCTACCCACACCTGCAGTGGCTGTACGGCGCGCTTGGGCTGG
                  **** .*.***** **..*.** **:**  ****************.**  *:**  ***

HsCD00583116      CCGTTATCGGTGCTTCTTCCTGTATTGTCCCTGCCTGCAGAAGTTTTGCTCCACTGGTGG
NM_003055.2       CTGTGATCGGCGCCAGCTCGTGCATCGTGCCCGCCTGCCGCTCCTTCGCGCCGCTAGTGG
                  * ** ***** ** :  ** ** ** ** ** ******.*.:  ** ** **.**.****

HsCD00583116      TTTCCCTTTGTGGATTGTGCTTCGGTATCGCCTTGGTTGACACTGCTCTGCTTCCAACCC
NM_003055.2       TCTCACTATGCGGCCTCTGTTTTGGCATAGCCCTAGTCGACACAGCACTGCTGCCCACGC
                  * **.**:** **. * ** ** ** **.*** *.** *****:**:***** **.** *

HsCD00583116      TGGCCTTTCTTGTCGATGTGAGACACGTTTCTGTCTATGGTTCCGTTTACGCTATCGCCG
NM_003055.2       TCGCCTTCCTGGTGGACGTGCGCCATGTCTCAGTCTATGGCAGCGTCTACGCCATCGCCG
                  * ***** ** ** ** ***.*.** ** **:******** : *** ***** *******

HsCD00583116      ACATTAGCTATTCTGTCGCATACGCTTTGGGACCTATCGTTGCTGGTCACATTGTCCATT
NM_003055.2       ACATCTCCTATTCGGTGGCCTACGCGCTCGGGCCCATAGTGGCAGGCCACATTGTGCACT
                  **** : ****** ** **.*****  * **.** **.** **:** ******** ** *

HsCD00583116      CACTGGGTTTCGAGCAACTGTCTCTTGGAATGGGTTTGGCCAACTTACTCTACGCTCCAG
NM_003055.2       CGCTGGGCTTTGAGCAGCTCAGCCTTGGCATGGGACTGGCCAACCTGCTCTATGCTCCCG
                  *.***** ** *****.** :  *****.*****: ******** *.***** *****.*

HsCD00583116      TTTTGCTGCTTTTAAGAAATGTCGGTCTCTTGACTAGATCCAGATCAGAGAGAGACGTTT
NM_003055.2       TCTTGCTGCTGCTCCGCAACGTGGGCCTCCTGACGCGCTCCCGTTCCGAGCGCGATGTGC
                  * ********  *..*.** ** ** *** **** .*.***.*:**.***.*.** **  

HsCD00583116      TGTTGGACGAACCCCCACAGGGTTTGTATGACGCCGTTAGACTGAGAGAGAGACCAGTCT
NM_003055.2       TGCTTGATGAGCCACCGCAAGGTCTGTACGATGCGGTGCGCCTGCGTGAGCGTCCTGTGT
                  ** * ** **.**.**.**.*** **** ** ** ** .*.***.*:***.*:**:** *

HsCD00583116      CCGGACAAGACGGTGAACCTAGATCTCCTCCCGGTCCATTCGATGCTTGCGAAGACGATT
NM_003055.2       CTGGCCAGGACGGCGAGCCTCGCAGCCCGCCTGGCCCTTTTGATGCGTGCGAGGACGACT
                  * **.**.***** **.***.*.:  ** ** ** **:** ***** *****.***** *

HsCD00583116      ACAACTACTATTACACCAGATCTGGTACCAGAGGTTCTCACCACCATCACCACCATCACC
NM_003055.2       ACAACTACTACTACACCCGCAGCTAG----------------------------------
                  ********** ******.*.:   .                                   

HsCD00583116      ACCATCACTAATAA
NM_003055.2       --------------

Pathways

Panther :

Muscarinic acetylcholine receptor 1 and 3 signaling pathway

Panther :

Muscarinic acetylcholine receptor 2 and 4 signaling pathway

Panther :

Nicotinic acetylcholine receptor signaling pathway

Reactome :

Acetylcholine Neurotransmitter Release Cycle

Reactome :

Neuronal System

Reactome :

Neurotransmitter Release Cycle

Reactome :

Transmission across Chemical Synapses

Protein

Structural Annotation Wiki :

TransportPDB-0034

HPRD :

08979

Clone

Cloning Information :

583285

Original Clone ID :

TPDB-0034

Experimental

TargetTrack - Experimental data :

TransportPDB-0034

Organism

Species Specific ID:

6572

Genome

Chromosome :

Map Location :

10q11.2

Ensembl :

ENSG00000187714

Reagents

Labome :

vesicular-acetylcholine-transporter-antibody