DNASU Plasmid | Caci_7372 (C. acidiphila DSM 44928 )<br>In pMCSG73 (Bacterial Expression)

Explanation of Terms

Gene:

Gene Symbol: Caci_7372

Gene Name: glycoside hydrolase family protein

Original Clone ID:

APC103186.103

Keyword:

None

Species:

Catenulispora acidiphila DSM 44928

Type:

cDNA

Vector Name:

pMCSG73

Format:

FUSION

Source:

Midwest Center for Structural Genomics

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

Argonne National Laboratory
Robert Jedrzejczak
PSI:Biology
Midwest Center for Structural Genomics

CaCD00583127

Price:

AVAILABLE

No restriction
Special MTA: PSI

Insert Sequence
Protein Sequence

Insert sequence: 978nts

Open reading frame: 5 to 982

TGCCGTGACCATCTTCGGACCGGACATATCGTCCTACCAGAGCGGGCTAGTCCTGTCCCG
GCTCTCGGACGCCGCGTTCATCATCGCGAAGACGACCGAAGGCACCTACTACACCGATGC
CGACTATCAGGCCTGGCGCAGCCAGGCCTCAGCACTCGGTAGACCGTTCATCTGGTACCA
CTTCCTGTCCGGGGAGAGCGCCGCGTCTCAGGCCGCGCACACAGCGGCCAACATCGGAGA
CCGCTCCCTGCCGGGCATGCTCGACGCCGAGCCGGCGGGCTCGTACTCCCCGTCCCTGGC
CCAGATCGTCGCCTACGTCGACGCCGCTCTCGCCGCGGGACTCAACCTGCGTCTGGTCTA
TCTGCCCCGGTGGTACTGGCAGCAGATGGGCTCGCCGAACCTGTCCGAACTCTCCGACCG
GGGCGTCTCGCTGGTCTCCTCGTCCTACCCGGGCGGCTCCGGCGGCCCGGGCAGCATCTA
TCCCGGGGACGACGCGGCCGGATGGCAGGCCTACGGCGGGATGACACCCCTGATCTACCA
GTTCACGAACCAGGCGTCCGACGGCGGACAGCAGCTGGACTACAACGCGTTCCGGGGCAC
GGCCGCACAACTGGTCACCGCACTGGGCCCCACCCATGTTTTGGAGGACGACACCATGCC
CGCATTCGCCACCGGTGAGATCACCCCCGGCGCCGGCGCCGTGACGATGGTGCTGCCGCC
GCCGGCGAACTACGGCGCCGCCGGCTGGGGCAACGTCTGGTTCAGCCTGGGCGCCGACTT
CGGCACGGCGACCGTCCGGGTCGCCATCTTCACCCACGGCCAGGGCTGGTCGCACATCTA
CGAGGACGTGGTGGTCGACGCCGCCGCCGATCGGGTGAACCCCTTCGGCGGACCGCTGCC
GACCGCCGTGCAGAAGATCAGCGTGACACGGAGGGGGAACCCGAACGTGCCGCTCGCCTA
CCTGGTCGAGGCAGTGCAGCGG

Protein sequence predicted based on nucleotide sequence

VTIFGPDISSYQSGLVLSRLSDAAFIIAKTTEGTYYTDADYQAWRSQASALGRPFIWYHF
LSGESAASQAAHTAANIGDRSLPGMLDAEPAGSYSPSLAQIVAYVDAALAAGLNLRLVYL
PRWYWQQMGSPNLSELSDRGVSLVSSSYPGGSGGPGSIYPGDDAAGWQAYGGMTPLIYQF
TNQASDGGQQLDYNAFRGTAAQLVTALGPTHVLEDDTMPAFATGEITPGAGAVTMVLPPP
ANYGAAGWGNVWFSLGADFGTATVRVAIFTHGQGWSHIYEDVVVDAAADRVNPFGGPLPT
AVQKISVTRRGNPNVPLAYLVEAVQR

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c156t,G52G

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Protein_Soluble

Find experimental protocols in MCSG-APC103186

Recommended expression in:

Not Applicable

Vector Name:

pMCSG73

Synonyms:

Sequencing Primer:

Forward: MCSG73F

Reverse: T7 terminator

Description:

Bacterial expression vector with a T7 promoter and TVMV-cleavable N-terminal NusA tag and TEV-cleavable N-terminal 6xHis and StrepII tag; Contains rate argU and ileX tRNA genes; ampicillin resistance in bacteria; ligation independent cloning.

Comments:

None

Size (bp):

6227

Parent Vector:

Name: pMCSG53

Description:

Properties:

bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker

Author Name:

Midwest Center for Structural Genomics
PSI:Biology
Midwest Center for Structural Genomics

Publications:

None

Vector Map:

Vector Sequence:

Sequence:

ATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATGTTATCCACTTCCAATATTGGATTGGAAGTACAGGTTCTCCTTCTCGAACTGTGGATGAGACCAGTGATGATGATGGTGGTGAGACTGGAAACGCACGGTTTCGTTGTTGTTATTACGGGCAGCCATAATCAGTGCTCCGGCTTTTTCGTCGGTCAACCCTTCGATATCAGCCAGATCATCAATGCCCTGTTCGGCGAGATCTTCCAGCGTACAAACGCCACGGGCGGCCAGTTTGAATGCCAAATCACGATCTACCCCTTCAAGGTTCAGCAGATCGTCAGCCGGTTTGTTATCACCGAGGCTTTCTTCCTGGGCCTGTGCAATGGTGGCCAGTGCATTTTTAGCACGCTCGCGCAGTGCTTCAACGGTCGGCTCATCAAGGCCTTCGATTTCCAACAGCTCTTTCATCGGCACATAGGCCAATTCTTCCAGCGTCGAGAAGCCTTCTTCTACCAGAACAGTCGCGAAGTCTTCGTCGATGTCGAGATATTTGGTGAAGGTGTCGATCGCTGCGTGCGCTTCCGCCTGATGCTTAGCTTGCAGGTCGTCAACGGTCATCACGTTGAGTTCCCAACCGCTCAGTTGCGAAGCCAGACGCACGTTCTGACCGTTACGGCCAATCGCCTGCGCCAGATTACCGGCTTCAACGGCGATGTCCATGGTGTGTTTATCTTCATCCACCACGATAGAAGCAACGTCTGCCGGTGCCATTGCGTTAATCACGAACTGCGCCGGGTTATCATCCCACAGGACGATATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTGCTTTAATTTCAATCACTTCTTCGCCGATTTCTGGCACTTCAATACGGAACAGTTCGATCAGCATTTCCGGCTTGGAACGAGTGACGAACAGTTGCGCGCCACGCGCTTCCGGGCGAACGGAATAGAGCACGCCACGAACGCGGTCGCCAGGGCGGAAGTTTTCACGCGGCAGCATATCTTCGCGCAGGATCACGGCTTCAGCGTTGTTGCCCAGATCCAGAGAGATGTTGTCGCGGTTTACTTTTTTCACCACGCCGGTGATGATTTCACCTTCGTGTTCACGGAACTGATCAACCACCATCGCACGTTCGGCTTCACGCACTTTCTGCACGATAACCTGTTTTGCCGTCTGGGTAGTGATACGGTCAAAGGTAACAGACTCAATCTGATCTTCAACGTAATCGCCCAGGTTCAGGCTTTCATCTTCATAACGTGCGGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATATGTATATCTCCTTCTTAAAGTTAAACAAAATTATTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGTGGATTCAGTCAGGCGTCCCATTATCAGTGCTTCAGGAAATGGGCGGATGGGAGTCCATAGAAATGGTTCGTAGGTATGCTCACCTTGCGCCTAATCATTTGACAGAGCATGCGAGGAAAATAGACGACATTTTTGGTGATAATGTCCCAAATATGTCCCACTCTGGAATTATGGAGGATATAAAGAAGGCGTAACTGATTGAATTGTAATGGCGCGCCCTGCAGGATTCGAACCTGCGGCCCACGACTTAGAAGGTCGTTGCTCTATCCAACTGAGCTAAGGGCGCGTTGATACCGCAATGCGGTGTAATCGCGTGAATTATACGGTCAACCCTTGCTGAGTCAATGGCTTTTGATCTCGCCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCAAAAAGGCTGACGATTTCTCGTCAGCCTTTGCTATATCTGGTGGCCCCTGCTGGACTTGAACCAGCGACCAAGCGATTATGAGTCGCCTGCTCTAACCACTGAGCTAAGGGGCCATGGTAGCGGATTATAAAGTAACTCCGTGTCGCAATCCGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTACAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAAATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTAAATTGTAAGCGTTAATGTGAACCATCACCCTAATCAAGTTTTTTGGGGTCGAGGTGCCGTAAAGCACTAAATCGGAACCCTAAAGGGAGCCCCCGATTTAGAGCTTGACGGGGAAAGCCGGCGAACGTGGCGAGAAAGGAAGGGAAGAAAGCGAAAGGAGCGGGCGCTAGGGCGCTGGCAAGTGTAGCGGTCACGCTGCGCGTAACCACCACACCCGCCGCGCTTAATGCGCCGCTACAGGGCGCGTCCCATTCGCCA

Vector Features:

Type

Name

Description

Start

End

bacterial origin

pBR322 origin

pBR322 origin of replication

4220

4808

ligation independent cloning

LIC

ligation independent cloning site

138

promoter

T7 promoter

1779

1795

protease cleavage site

TVMV

TVMV protease cleavage site

218

238

protease cleavage site

TEV

TEV protease cleavage site

155

175

repressor binding site

lac operator

1754

1776

repressor protein gene

LacI

LacI CDS

2832

3791

selectable marker

AmpR

ampicillin resistance gene

4979

5839

tRNA

ileX

ileX tRNA gene

2378

2529

tRNA

argU

argU tRNA

1862

2218

tag

NusA

N-terminal NusA

249

1708

tag

His

N-terminal 6xHis tag

200

217

tag

strep

N-terminal StrepII tag

176

199

trxn termination sequence

T7 term

T7 terminator

Gene

Gene Symbol :

Caci_7372

GENEID :

8338742

Locus Tag :

Caci_7372

GI :

256362702

Target GenBank:

None

Protein

SMART domain :

Glyco_25 : Glycosyl hydrolases family 25

Structural Annotation Wiki :

MCSG-APC103186

Clone

Cloning Information :

603322

Original Clone ID :

APC103186.103

Experimental

TargetTrack - Experimental data :

MCSG-APC103186

Organism

Species Specific ID:

8338742

Caci_7372 (C. acidiphila DSM 44928 )In pMCSG73 (Bacterial Expression)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Protein

Clone

Experimental

Organism

Caci_7372 (C. acidiphila DSM 44928 )
In pMCSG73 (Bacterial Expression)