DNASU Plasmid | TRIM31 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TRIM31

Gene Name: tripartite motif containing 31

Original Clone ID:

HR9052A-66-385-15.4

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00584045

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 966nts

Open reading frame: 27 to 992

Reference Sequence Alignment

GCCATCACCATCACCATCACAGCCATATGAGGTTCAACTCGCTGTTGCGGAATCTGGTGG
AGAAAATCCAAGCTCTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTACATGCC
CGAGGCACCAGGAGATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTG
TGTGTCGTGAATCCAAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCC
AGAATTATCAGGGGCAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGA
CAGTACAAGTGAAGGCACAAGGTGTACACAGGGTCGATGTCTTCACGGACCAGGTAGAAC
ATGAGAAGCAAAGGATCCTCACAGAATTTGAACTCCTGCATCAAGTCCTAGAGGAGGAGA
AGAATTTCCTGCTATCACGGATTTACTGGCTGGGTCATGAGGGAACGGAAGCAGGGAAAC
ACTATGTTGCCTCCACTGAGCCACAGTTGAACGATCTCAAGAAGCTCATTGATTCCCTGA
AGACCAAGCAGAACATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCA
GAAGTGAAGAGTTTCAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAAC
TCAGTGAAGCAAAATCAAGACATGACTCCATCACAGGGAGCCTGAAAAAATTCAAAGACC
AACTCCAGGCTGATAGGAAAAAAGATGAAAACAGATTCTTCAAAAGCATGAATAAAAATG
ACATGAAGAGCTGGGGCTTGTTACAGAAAAATAATCATAAAATGAACAAAACCTCAGAGC
CCGGGTCATCTTCTGCAGGCGGCAGAACTACATCGGGGCCACCAAATCACCACTCTTCAG
CCCCATCCCACTCCCTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAGTATGTC
TCCTGGCCTCTTATGATGAGATTTCTGGTTAG

Protein sequence predicted based on nucleotide sequence

MRFNSLLRNLVEKIQALQASEVQSKRKEATCPRHQEMFHYFCEDDGKFLCFVCRESKDHK
SHNVSLIEEAAQNYQGQIQEQIQVLQQKEKETVQVKAQGVHRVDVFTDQVEHEKQRILTE
FELLHQVLEEEKNFLLSRIYWLGHEGTEAGKHYVASTEPQLNDLKKLIDSLKTKQNMPPR
QLLEDIKVVLCRSEEFQFLNPTPVPLELEKKLSEAKSRHDSITGSLKKFKDQLQADRKKD
ENRFFKSMNKNDMKSWGLLQKNNHKMNKTSEPGSSSAGGRTTSGPPNHHSSAPSHSLFRA
SSAGKVTFPVCLLASYDEISG*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g447a,A149A;g502a,V168I;

a678g,L226L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR9052A

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

TRIM31

Symbol Nomenclature :

TRIM31

SYNONYM :

C6orf13; HCG1; HCGI; RNF

Designation :

E3 ubiquitin-protein ligase TRIM31|ring finger protein|tripartite motif-containing 31 gamma|tripartite motif-containing protein 31

Full Nomenclature :

tripartite motif containing 31

GENEID :

11074

Locus Tag :

DAAP-112O13.2

GenBank Accession :

NM_052816

HGNC :

16289

MIM :

609316

Vega :

OTTHUMG00000031064

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00584045      ------------------------------------------------------------
NM_007028.3       ATGGCCAGTGGGCAGTTTGTGAACAAACTGCAAGAGGAAGTGATCTGCCCCATCTGCCTG
                                                                              

HsCD00584045      ------------------------------------------------------------
NM_007028.3       GACATTCTGCAGAAACCTGTCACCATCGACTGTGGGCACAATTTCTGCCTCAAATGCATC
                                                                              

HsCD00584045      ----------------------------------------------GCCATCACCATCAC
NM_007028.3       ACTCAGATTGGGGAAACATCATGTGGATTTTTCAAATGTCCCCTCTGCAAAACTTCCGTA
                                                                **.*:..  .  :.

HsCD00584045      CATCACAGCCATATGAGGTTCAACTCGCTGTTGCGGAATCTGGTGGAGAAAATCCAAGCT
NM_007028.3       AGGAAGAACGCAATCAGGTTCAACTCGCTGTTGCGGAATCTGGTGGAGAAAATCCAAGCT
                  .. .* *.* .:** *********************************************

HsCD00584045      CTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTACATGCCCGAGGCACCAGGAG
NM_007028.3       CTACAAGCCTCTGAGGTGCAGTCCAAAAGGAAAGAGGCTACATGCCCGAGGCACCAGGAG
                  ************************************************************

HsCD00584045      ATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTGTCGTGAATCC
NM_007028.3       ATGTTCCACTATTTCTGCGAGGATGATGGGAAGTTCCTCTGTTTTGTGTGTCGTGAATCC
                  ************************************************************

HsCD00584045      AAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG
NM_007028.3       AAGGACCACAAATCCCATAATGTCAGCTTGATCGAAGAAGCTGCCCAGAATTATCAGGGG
                  ************************************************************

HsCD00584045      CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAG
NM_007028.3       CAGATTCAAGAGCAGATCCAAGTCTTGCAGCAAAAGGAGAAGGAGACAGTACAAGTGAAG
                  ************************************************************

HsCD00584045      GCACAAGGTGTACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGG
NM_007028.3       GCACAAGGTGTACACAGGGTCGATGTCTTCACGGACCAGGTAGAACATGAGAAGCAAAGG
                  ************************************************************

HsCD00584045      ATCCTCACAGAATTTGAACTCCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTA
NM_007028.3       ATCCTCACAGAATTTGAACTCCTGCATCAAGTCCTAGAGGAGGAGAAGAATTTCCTGCTA
                  ************************************************************

HsCD00584045      TCACGGATTTACTGGCTGGGTCATGAGGGAACGGAAGCAGGGAAACACTATGTTGCCTCC
NM_007028.3       TCACGGATTTACTGGCTGGGTCATGAGGGAACGGAAGCGGGGAAACACTATGTTGCCTCC
                  **************************************.*********************

HsCD00584045      ACTGAGCCACAGTTGAACGATCTCAAGAAGCTCATTGATTCCCTGAAGACCAAGCAGAAC
NM_007028.3       ACTGAGCCACAGTTGAACGATCTCAAGAAGCTCGTTGATTCCCTGAAGACCAAGCAGAAC
                  *********************************.**************************

HsCD00584045      ATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAGTGAAGAGTTT
NM_007028.3       ATGCCACCCAGGCAGCTGCTGGAGGATATCAAAGTCGTCTTGTGCAGAAGTGAAGAGTTT
                  ************************************************************

HsCD00584045      CAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA
NM_007028.3       CAGTTTCTCAACCCAACCCCTGTTCCTCTGGAACTGGAGAAAAAACTCAGTGAAGCAAAA
                  ************************************************************

HsCD00584045      TCAAGACATGACTCCATCACAGGGAGCCTGAAAAAATTCAAAGACCAACTCCAGGCTGAT
NM_007028.3       TCAAGACATGACTCCATCACAGGGAGCCTAAAAAAATTCAAAGACCAACTCCAGGCTGAT
                  *****************************.******************************

HsCD00584045      AGGAAAAAAGATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGG
NM_007028.3       AGGAAAAAAGATGAAAACAGATTCTTCAAAAGCATGAATAAAAATGACATGAAGAGCTGG
                  ************************************************************

HsCD00584045      GGCTTGTTACAGAAAAATAATCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCT
NM_007028.3       GGCTTGTTACAGAAAAATAATCATAAAATGAACAAAACCTCAGAGCCCGGGTCATCTTCT
                  ************************************************************

HsCD00584045      GCAGGCGGCAGAACTACATCGGGGCCACCAAATCACCACTCTTCAGCCCCATCCCACTCC
NM_007028.3       GCAGGCGGCAGAACTACATCGGGGCCACCAAATCACCACTCTTCAGCCCCATCCCACTCC
                  ************************************************************

HsCD00584045      CTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAGTATGTCTCCTGGCCTCTTAT
NM_007028.3       CTGTTTCGGGCCTCGTCTGCTGGGAAAGTCACTTTTCCAGTATGTCTCCTGGCCTCTTAT
                  ************************************************************

HsCD00584045      GATGAGATTTCTGGTTAG------------------------------------------
NM_007028.3       GATGAGATTTCTGGTCAAGGAGCGAGCTCTCAGGATACGAAGACATTTGACGTTGCGCTG
                  *************** *.                                          

HsCD00584045      ------------------------------------------------------------
NM_007028.3       TCCGAGGAGCTCCATGCGGCACTGAGTGAGTGGCTGACAGCGATCCGGGCTTGGTTTTGT
                                                                              

HsCD00584045      ------------------
NM_007028.3       GAGGTTCCTTCAAGCTAA