DNASU Plasmid | SNX18 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: SNX18

Gene Name: sorting nexin 18

Original Clone ID:

HR9055-227-628-15.3

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00584047

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1212nts

Open reading frame: 10 to 1221

Reference Sequence Alignment

CACAGCCATATGCGCAACCTCAATCGCTTCTCCACCTTCGTCGAGTCCGGCGGGGAGGCC
TTCGTGCTGGGGGAGGCGTCAGGCTTCGTGAAGGACGGGGACAAGCTGTGCGTGGTGCTG
GGGCCCTATGGCCCCGAGTGGCAGGAGAACCCCTACCCGTTCCAGTGCACCATCGACGAC
CCCACCAAGCAGACCAAGTTCAAGGGCATGAAGAGCTACATCTCCTACAAGCTGGTGCCC
ACGCACACGCAGGTGCCGGTGCATCGGCGCTACAAGCACTTCGACTGGCTGTACGCGCGC
CTGGCGGAGAAGTTCCCGGTCATCTCCGTGCCCCACCTGCCCGAGAAGCAGGCCACCGGC
CGCTTCGAGGAGGACTTCATCTCTAAGCGCAGGAAGGGCCTGATCTGGTGGATGAACCAC
ATGGCCAGCCACCCAGTGCTGGCGCAGTGCGACGTCTTCCAGCACTTCCTGACGTGCCCC
AGCAGCACCGACGAGAAAGCCTGGAAGCAGGGCAAGAGGAAGGCCGAGAAGGACGAGATG
GTGGGCGCCAACTTCTTCCTGACCCTTAGCACGCCCCCCGCCGCTGCCCTTGACCTGCAG
GAGGTGGAGAGCAAGATAGACGGCTTCAAGTGCTTCACCAAGAAGATGGACGACAGCGCG
CTGCAGCTCAACCACACGGCCAACGAGTTCGCGCGCAAGCAGGTGACCGGCTTCAAAAAG
GAGTATCAGAAGGTGGGCCAGTCCTTCCGCGGCCTCAGCCAGGCCTTTGAGCTGGACCAG
CAGGCCTTCTCGGTGGGCCTGAACCAGGCTATCGCCTTCACCGGAGATGCCTATGACGCC
ATTGGCGAGCTCTTCGCGGAGCAGCCCAGGCAGGACCTGGATCCCGTCATGGACCTATTA
GCGCTGTATCAGGGGCATCTGGCTAACTTCCCGGACATCATCCACGTTCAGAAAGGTAAA
GCCTGGCCCTTAGAGCAGGTGATATGGAGTGTATTGTGCAGGCTGAAAGGGGCGACTTTG
ACAGCAGTACCACTGTGGGTTTCAGAATCATATTCTACAGGTGAGGAAGCGAGCAGAGAC
GTGGACGCCTGGGTCTTTTCCCTAGAGTGTAAGTTGGATTGCTCGACAGGCAGCTTCCTC
CTCGAGTATCTTGCATTAGGGAATGAGTACTCTTTCTCGAAGGTTCAAAGAGTACCTTTG
ATGACAGTGCTATCATTTTAG

Protein sequence predicted based on nucleotide sequence

MRNLNRFSTFVESGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPTK
QTKFKGMKSYISYKLVPTHTQVPVHRRYKHFDWLYARLAEKFPVISVPHLPEKQATGRFE
EDFISKRRKGLIWWMNHMASHPVLAQCDVFQHFLTCPSSTDEKAWKQGKRKAEKDEMVGA
NFFLTLSTPPAAALDLQEVESKIDGFKCFTKKMDDSALQLNHTANEFARKQVTGFKKEYQ
KVGQSFRGLSQAFELDQQAFSVGLNQAIAFTGDAYDAIGELFAEQPRQDLDPVMDLLALY
QGHLANFPDIIHVQKGKAWPLEQVIWSVLCRLKGATLTAVPLWVSESYSTGEEASRDVDA
WVFSLECKLDCSTGSFLLEYLALGNEYSFSKVQRVPLMTVLSF*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a34g,K12E;c609a,I203I

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR9055

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

SNX18

Symbol Nomenclature :

SNX18

SYNONYM :

SH3PX2; SH3PXD3B; SNAG1

Designation :

SH3 and PX domain-containing protein 3B|sorting nexin associated golgi protein 1|sorting nexin-18|sorting nexin-associated Golgi protein 1

Full Nomenclature :

sorting nexin 18

GENEID :

112574

GI :

157057543

GenBank Accession :

NM_052870

HGNC :

19245

Vega :

OTTHUMG00000096994

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00584047        ------------------------------------------------------------
NM_052870.2         ATGGCGCTGCGCGCCCGGGCGCTGTACGACTTCAGGTCGGAGAACCCAGGAGAGATCTCG
NM_001145427.1      ATGGCGCTGCGCGCCCGGGCGCTGTACGACTTCAGGTCGGAGAACCCAGGAGAGATCTCG
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         CTGCGAGAGCACGAGGTGCTGAGCCTGTGCAGCGAGCAGGACATCGAGGGCTGGCTCGAG
NM_001145427.1      CTGCGAGAGCACGAGGTGCTGAGCCTGTGCAGCGAGCAGGACATCGAGGGCTGGCTCGAG
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         GGGGTCAACAGCCGCGGCGACCGCGGCCTCTTCCCGGCCTCCTATGTGCAGGTGATCCGC
NM_001145427.1      GGGGTCAACAGCCGCGGCGACCGCGGCCTCTTCCCGGCCTCCTATGTGCAGGTGATCCGC
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         GCCCCCGAGCCTGGCCCGGCGGGAGACGGCGGCCCGGGCGCCCCGGCCCGCTACGCCAAT
NM_001145427.1      GCCCCCGAGCCTGGCCCGGCGGGAGACGGCGGCCCGGGCGCCCCGGCCCGCTACGCCAAT
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         GTGCCCCCCGGGGGCTTCGAGCCCCTGCCTGTCGCGCCCCCCGCCTCCTTCAAGCCGCCG
NM_001145427.1      GTGCCCCCCGGGGGCTTCGAGCCCCTGCCTGTCGCGCCCCCCGCCTCCTTCAAGCCGCCG
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         CCTGACGCCTTCCAGGCGCTGCTGCAGCCACAGCAGGCGCCGCCTCCGAGCACCTTCCAG
NM_001145427.1      CCTGACGCCTTCCAGGCGCTGCTGCAGCCACAGCAGGCGCCGCCTCCGAGCACCTTCCAG
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         CCGCCCGGCGCGGGCTTCCCGTACGGCGGGGGCGCCCTGCAGCCGTCGCCTCAGCAGCTC
NM_001145427.1      CCGCCCGGCGCGGGCTTCCCGTACGGCGGGGGCGCCCTGCAGCCGTCGCCTCAGCAGCTC
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         TACGGCGGCTACCAGGCCAGCCAAGGCAGCGATGATGACTGGGACGACGAGTGGGACGAC
NM_001145427.1      TACGGCGGCTACCAGGCCAGCCAAGGCAGCGATGATGACTGGGACGACGAGTGGGACGAC
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         AGCTCCACGGTGGCGGACGAGCCGGGCGCTCTGGGCAGCGGAGCATACCCGGACCTCGAC
NM_001145427.1      AGCTCCACGGTGGCGGACGAGCCGGGCGCTCTGGGCAGCGGAGCATACCCGGACCTCGAC
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         GGCTCGTCTTCGGCGGGTGTGGGCGCAGCCGGCCGCTACCGCCTGTCCACGCGCTCCGAC
NM_001145427.1      GGCTCGTCTTCGGCGGGTGTGGGCGCAGCCGGCCGCTACCGCCTGTCCACGCGCTCCGAC
                                                                                

HsCD00584047        ------------------------------------------------------------
NM_052870.2         CTGTCCCTGGGTTCCCGCGGCGGCTCGGTCCCCCCGCAGCACCACCCGTCGGGGCCCAAG
NM_001145427.1      CTGTCCCTGGGTTCCCGCGGCGGCTCGGTCCCCCCGCAGCACCACCCGTCGGGGCCCAAG
                                                                                

HsCD00584047        ------CACAGCCATATGCGCAACCTCAATCGCTTCTCCACCTTCGTCGAGTCCGGCGGG
NM_052870.2         AGCTCGGCCACCGTGAGCCGCAACCTCAATCGCTTCTCCACCTTCGTCAAGTCCGGCGGG
NM_001145427.1      AGCTCGGCCACCGTGAGCCGCAACCTCAATCGCTTCTCCACCTTCGTCAAGTCCGGCGGG
                           .** * : *  ******************************.***********

HsCD00584047        GAGGCCTTCGTGCTGGGGGAGGCGTCAGGCTTCGTGAAGGACGGGGACAAGCTGTGCGTG
NM_052870.2         GAGGCCTTCGTGCTGGGGGAGGCGTCAGGCTTCGTGAAGGACGGGGACAAGCTGTGCGTG
NM_001145427.1      GAGGCCTTCGTGCTGGGGGAGGCGTCAGGCTTCGTGAAGGACGGGGACAAGCTGTGCGTG
                    ************************************************************

HsCD00584047        GTGCTGGGGCCCTATGGCCCCGAGTGGCAGGAGAACCCCTACCCGTTCCAGTGCACCATC
NM_052870.2         GTGCTGGGGCCCTATGGCCCCGAGTGGCAGGAGAACCCCTACCCGTTCCAGTGCACCATC
NM_001145427.1      GTGCTGGGGCCCTATGGCCCCGAGTGGCAGGAGAACCCCTACCCGTTCCAGTGCACCATC
                    ************************************************************

HsCD00584047        GACGACCCCACCAAGCAGACCAAGTTCAAGGGCATGAAGAGCTACATCTCCTACAAGCTG
NM_052870.2         GACGACCCCACCAAGCAGACCAAGTTCAAGGGCATGAAGAGCTACATCTCCTACAAGCTG
NM_001145427.1      GACGACCCCACCAAGCAGACCAAGTTCAAGGGCATGAAGAGCTACATCTCCTACAAGCTG
                    ************************************************************

HsCD00584047        GTGCCCACGCACACGCAGGTGCCGGTGCATCGGCGCTACAAGCACTTCGACTGGCTGTAC
NM_052870.2         GTGCCCACGCACACGCAGGTGCCGGTGCATCGGCGCTACAAGCACTTCGACTGGCTGTAC
NM_001145427.1      GTGCCCACGCACACGCAGGTGCCGGTGCATCGGCGCTACAAGCACTTCGACTGGCTGTAC
                    ************************************************************

HsCD00584047        GCGCGCCTGGCGGAGAAGTTCCCGGTCATCTCCGTGCCCCACCTGCCCGAGAAGCAGGCC
NM_052870.2         GCGCGCCTGGCGGAGAAGTTCCCGGTCATCTCCGTGCCCCACCTGCCCGAGAAGCAGGCC
NM_001145427.1      GCGCGCCTGGCGGAGAAGTTCCCGGTCATCTCCGTGCCCCACCTGCCCGAGAAGCAGGCC
                    ************************************************************

HsCD00584047        ACCGGCCGCTTCGAGGAGGACTTCATCTCTAAGCGCAGGAAGGGCCTGATCTGGTGGATG
NM_052870.2         ACCGGCCGCTTCGAGGAGGACTTCATCTCTAAGCGCAGGAAGGGCCTGATCTGGTGGATG
NM_001145427.1      ACCGGCCGCTTCGAGGAGGACTTCATCTCTAAGCGCAGGAAGGGCCTGATCTGGTGGATG
                    ************************************************************

HsCD00584047        AACCACATGGCCAGCCACCCAGTGCTGGCGCAGTGCGACGTCTTCCAGCACTTCCTGACG
NM_052870.2         AACCACATGGCCAGCCACCCAGTGCTGGCGCAGTGCGACGTCTTCCAGCACTTCCTGACG
NM_001145427.1      AACCACATGGCCAGCCACCCAGTGCTGGCGCAGTGCGACGTCTTCCAGCACTTCCTGACG
                    ************************************************************

HsCD00584047        TGCCCCAGCAGCACCGACGAGAAAGCCTGGAAGCAGGGCAAGAGGAAGGCCGAGAAGGAC
NM_052870.2         TGCCCCAGCAGCACCGACGAGAAAGCCTGGAAGCAGGGCAAGAGGAAGGCCGAGAAGGAC
NM_001145427.1      TGCCCCAGCAGCACCGACGAGAAAGCCTGGAAGCAGGGCAAGAGGAAGGCCGAGAAGGAC
                    ************************************************************

HsCD00584047        GAGATGGTGGGCGCCAACTTCTTCCTGACCCTTAGCACGCCCCCCGCCGCTGCCCTTGAC
NM_052870.2         GAGATGGTGGGCGCCAACTTCTTCCTGACCCTTAGCACGCCCCCCGCCGCTGCCCTTGAC
NM_001145427.1      GAGATGGTGGGCGCCAACTTCTTCCTGACCCTTAGCACGCCCCCCGCCGCTGCCCTTGAC
                    ************************************************************

HsCD00584047        CTGCAGGAGGTGGAGAGCAAGATAGACGGCTTCAAGTGCTTCACCAAGAAGATGGACGAC
NM_052870.2         CTGCAGGAGGTGGAGAGCAAGATCGACGGCTTCAAGTGCTTCACCAAGAAGATGGACGAC
NM_001145427.1      CTGCAGGAGGTGGAGAGCAAGATCGACGGCTTCAAGTGCTTCACCAAGAAGATGGACGAC
                    ***********************.************************************

HsCD00584047        AGCGCGCTGCAGCTCAACCACACGGCCAACGAGTTCGCGCGCAAGCAGGTGACCGGCTTC
NM_052870.2         AGCGCGCTGCAGCTCAACCACACGGCCAACGAGTTCGCGCGCAAGCAGGTGACCGGCTTC
NM_001145427.1      AGCGCGCTGCAGCTCAACCACACGGCCAACGAGTTCGCGCGCAAGCAGGTGACCGGCTTC
                    ************************************************************

HsCD00584047        AAAAAGGAGTATCAGAAGGTGGGCCAGTCCTTCCGCGGCCTCAGCCAGGCCTTTGAGCTG
NM_052870.2         AAAAAGGAGTATCAGAAGGTGGGCCAGTCCTTCCGCGGCCTCAGCCAGGCCTTTGAGCTG
NM_001145427.1      AAAAAGGAGTATCAGAAGGTGGGCCAGTCCTTCCGCGGCCTCAGCCAGGCCTTTGAGCTG
                    ************************************************************

HsCD00584047        GACCAGCAGGCCTTCTCGGTGGGCCTGAACCAGGCTATCGCCTTCACCGGAGATGCCTAT
NM_052870.2         GACCAGCAGGCCTTCTCGGTGGGCCTGAACCAGGCTATCGCCTTCACCGGAGATGCCTAT
NM_001145427.1      GACCAGCAGGCCTTCTCGGTGGGCCTGAACCAGGCTATCGCCTTCACCGGAGATGCCTAT
                    ************************************************************

HsCD00584047        GACGCCATTGGCGAGCTCTTCGCGGAGCAGCCCAGGCAGGACCTGGATCCCGTCATGGAC
NM_052870.2         GACGCCATTGGCGAGCTCTTCGCGGAGCAGCCCAGGCAGGACCTGGATCCCGTCATGGAC
NM_001145427.1      GACGCCATTGGCGAGCTCTTCGCGGAGCAGCCCAGGCAGGACCTGGATCCCGTCATGGAC
                    ************************************************************

HsCD00584047        CTATTAGCGCTGTATCAGGGGCATCTGGCTAACTTCCCGGACATCATCCACGTTCAGAAA
NM_052870.2         CTATTAGCGCTGTATCAGGGGCATCTGGCTAACTTCCCGGACATCATCCACGTTCAGAAA
NM_001145427.1      CTATTAGCGCTGTATCAGGGGCATCTGGCTAACTTCCCGGACATCATCCACGTTCAGAAA
                    ************************************************************

HsCD00584047        GGTAAAGCCTGGCCCTTAGAGCAGGTGATATGGAGTGTATTGTGCAGGCTGAAAGGGGCG
NM_052870.2         GGTAAAGCCTGGCCCTTAGAGCAGGTGATATGGAGTGTATTGTGCAGGCTGAAAGGGGCG
NM_001145427.1      GGTAAAGCCTGGCCCTTAGAGCAGGTGATATGGAGTGTATTGTGCAGGCTGAAAGGGGCG
                    ************************************************************

HsCD00584047        ACTTTGACAGCAGTACCACTGTGGGTTTCAGAATCATATTCTACAGGTGAGGAAGCGAGC
NM_052870.2         ACTTTGACAGCAGTACCACTGTGGGTTTCAGAATCATATTCTACAGGTGAGGAAGCGAGC
NM_001145427.1      ACTTTGACAGCAGTACCACTGTGGGTTTCAGAATCATATTCTACAGGTGAGGAAGCGAGC
                    ************************************************************

HsCD00584047        AGAGACGTGGACGCCTGGGTCTTTTCCCTAGAGTGTAAGTTGGATTGCTCGACAGGCAGC
NM_052870.2         AGAGACGTGGACGCCTGGGTCTTTTCCCTAGAGTGTAAGTTGGATTGCTCGACAGGCAGC
NM_001145427.1      AGAGACGTGGACGCCTGGGTCTTTTCCCTAGAGTGA------------------------
                    ***********************************:                        

HsCD00584047        TTCCTCCTCGAGTATCTTGCATTAGGGAATGAGTACTCTTTCTCGAAGGTTCAAAGAGTA
NM_052870.2         TTCCTCCTCGAGTATCTTGCATTAGGGAATGAGTACTCTTTCTCGAAGGTTCAAAGAGTA
NM_001145427.1      ------------------------------------------------------------
                                                                                

HsCD00584047        CCTTTGATGACAGTGCTATCATTTTAG
NM_052870.2         CCTTTGATGACAGTGCTATCATTTTAG
NM_001145427.1      ---------------------------