DNASU Plasmid | TBC1D17 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: TBC1D17

Gene Name: TBC1 domain family, member 17

Original Clone ID:

HR8990B-280-599-15.2

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00584119

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 966nts

Open reading frame: 43 to 1008

Reference Sequence Alignment

AGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGCCAGTTACAGAGGAG
GAGTGGGCACGCCACGTGGGCCCTGAAGGTCGCCTGCAGCAGGTCCCTGAGCTGAAGAAC
CGGATCTTCTCGGGGGGTCTGAGCCCCAGCCTGCGGCGCGAGGCCTGGAAGTTCCTCCTA
GGGTACCTCAGCTGGGAAGGCACAGCTGAGGAGCACAAGGCCCACATACGCAAGAAAACG
GATGAGTATTTCCGCATGAAGCTGCAGTGGAAATCTGTGAGCCCTGAGCAGGAGCGGAGA
AACTCACTTCTGCATGGATACCTCAGCCTCATCGAAAGGGATGTGAGCCGCACTGACAGG
ACCAACAAGTTCTACGAGGGTCCCGAGAACCCGGGGCTGGGCCTGCTGAACGATATCCTC
CTCACCTACTGCATGTATCACTTCGACCTCGGCTACGTCCAGGGCATGAGTGATCTTCTC
TCCCCGATCCTCTACGTCATTCAGAACGAGGTGGATGCTTTCTGGTGTTTCTGTGGCTTC
ATGGAGCTCGTGCAAGGGAACTTTGAAGAGAGCCAGGAGACCATGAAGCGGCAACTCGGG
CGACTGCTGCTGCTCCTGAGGGTGCTGGACCCCCTGCTCTGCGACTTCCTGGATTCCCAG
GACTCCGGCTCTCTCTGCTTCTGTTTCCGGTGGCTGCTCATCTGGTTCAAGAGGGAATTC
CCCTTCCCGGATGTCCTTCGGCTGTGGGAGGTGCTGTGGACAGGGCTCCCTGGCCCCAAT
CTGCACCTGCTGGTGGCCTGCGCCATCCTGGACATGGAGAGGGACACCCTCATGCTGTCC
GGCTTCGGCTCCAATGAGATCCTCAAGCACATCAACGAGCTGACTATGAAGCTGAGCGTG
GAGGACGTGCTGACCCGCGCCGAGGCCCTGCACCGCCAGCTAACCGCCTGCCCCGAGCTG
CCCCACAACGTGCAGGAGATCCTGGGGCTGGCCCCGCCCGCAGAGTAGCTCGAGATCCGG
CTGCTAACAAAGCCCGAAAG

Protein sequence predicted based on nucleotide sequence

MPVTEEEWARHVGPEGRLQQVPELKNRIFSGGLSPSLRREAWKFLLGYLSWEGTAEEHKA
HIRKKTDEYFRMKLQWKSVSPEQERRNSLLHGYLSLIERDVSRTDRTNKFYEGPENPGLG
LLNDILLTYCMYHFDLGYVQGMSDLLSPILYVIQNEVDAFWCFCGFMELVQGNFEESQET
MKRQLGRLLLLLRVLDPLLCDFLDSQDSGSLCFCFRWLLIWFKREFPFPDVLRLWEVLWT
GLPGPNLHLLVACAILDMERDTLMLSGFGSNEILKHINELTMKLSVEDVLTRAEALHRQL
TACPELPHNVQEILGLAPPAE*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

g281t,R94L

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR8990B

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

TBC1D17

Symbol Nomenclature :

TBC1D17

Designation :

TBC1 domain family member 17

Full Nomenclature :

TBC1 domain family, member 17

GENEID :

79735

GI :

270265906

GenBank Accession :

NM_024682

HGNC :

25699

Vega :

OTTHUMG00000183376

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      ------------------------------------------------------------
NM_024682.2         ATGGAAGGAGCCGGCTACAGGGTGGTGTTTGAGAAGGGCGGAGTGTACCTGCACACCAGC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      ---------------------------------------ATGGAAGGAGCCGGCTACAGG
NM_024682.2         GCTAAGAAGTATCAGGACCGAGACTCTCTCATCGCTGGTGTCATCCGTGTCGTGGAAAAG
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      GACAATGACGTCCTCCTGCACTGGGCTCCTGTAGAGGAGGCTGGAGATTCCACCCAAATC
NM_024682.2         GACAATGACGTCCTCCTGCACTGGGCTCCTGTAGAGGAGGCTGGAGATTCCACCCAAATC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      CTCTTCTCCAAGAAGGACTCCAGTGGGGGTGACTCATGTGCTTCTGAGGAGGAACCAACC
NM_024682.2         CTCTTCTCCAAGAAGGACTCCAGTGGGGGTGACTCATGTGCTTCTGAGGAGGAACCAACC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      TTTGACCCCGGCTATGAACCTGACTGGGCTGTCATCAGCACTGTGCGGCCACAGCTCTGC
NM_024682.2         TTTGACCCCGGCTATGAACCTGACTGGGCTGTCATCAGCACTGTGCGGCCACAGCTCTGC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      CACTCAGAGCCCACGAGAGGTGCAGAGCCCAGCTGCCCCCAGGGCTCCTGGGCCTTCTCA
NM_024682.2         CACTCAGAGCCCACGAGAGGTGCAGAGCCCAGCTGCCCCCAGGGCTCCTGGGCCTTCTCA
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      GTGAGTCTGGGGGAGCTAAAGTCCATCCGCCGCTCCAAGCCAGGCCTCAGCTGGGCCTAC
NM_024682.2         GTGAGTCTGGGGGAGCTAAAGTCCATCCGCCGCTCCAAGCCAGGCCTCAGCTGGGCCTAC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      CTGGTTCTGGTGACCCAGGCTGGAGGTTCCCTGCCCGCACTGCACTTCCACCGCGGGGGC
NM_024682.2         CTGGTTCTGGTGACCCAGGCTGGAGGTTCCCTGCCCGCACTGCACTTCCACCGCGGGGGC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      ACCCGCGCCCTGCTCCGCGTCCTCAGCCGCTACCTGCTGTTGGCCAGCTCCCCGCAGGAC
NM_024682.2         ACCCGCGCCCTGCTCCGCGTCCTCAGCCGCTACCTGCTGTTGGCCAGCTCCCCGCAGGAC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      TCCCGCCTCTACCTTGTCTTCCCCCACGACTCCTCTGCTCTCTCCAACTCCTTCCACCAC
NM_024682.2         TCCCGCCTCTACCTTGTCTTCCCCCACGACTCCTCTGCTCTCTCCAACTCCTTCCACCAC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      CTGCAGCTCTTTGACCAGGACAGCTCCAATGTGGTGTCACGCTTCCTCCAGGATCCCTAC
NM_024682.2         CTGCAGCTCTTTGACCAGGACAGCTCCAATGTGGTGTCACGCTTCCTCCAGGATCCCTAC
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      TCCACCACCTTCAGCAGCTTCTCCCGAGTGACCAACTTCTTCCGGGGTGCCCTGCAGCCA
NM_024682.2         TCCACCACCTTCAGCAGCTTCTCCCGAGTGACCAACTTCTTCCGGGGTGCCCTGCAGCCA
                                                                                

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      CAGCCTGAGGGAGCCGCCTCCGACCTTCCCCCGCCACCCGACGATGAGCCCGAGCCTGGA
NM_024682.2         CAGCCTGAGGGAGCCGCCTCCGACCTTCCCCCGCCACCCGACGATGAGCCCGAGCCTGGA
                                                                                

HsCD00584119        ------------AGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGCCA
NM_001168222.1      TTCGAGGTCATTTCCTGTGTGGAGCTGGGGCCTCGGCCAACCGTGGAGCGGGGCCCTCCA
NM_024682.2         TTCGAGGTCATTTCCTGTGTGGAGCTGGGGCCTCGGCCAACCGTGGAGCGGGGCCCTCCA
                                :  :*: : .. .**** *.**. *.:..* :  * .*  . .  ***

HsCD00584119        GTTACAGAGGAGGAGTGGGCACGCCACGTGGGCCCTGAAGGTCGCCTGCAGCAGGTCCCT
NM_001168222.1      GTTACAGAGGAGGAGTGGGCACGCCACGTGGGCCCTGAAGGTCGCCTGCAGCAGGTCCCT
NM_024682.2         GTTACAGAGGAGGAGTGGGCACGCCACGTGGGCCCTGAAGGTCGCCTGCAGCAGGTCCCT
                    ************************************************************

HsCD00584119        GAGCTGAAGAACCGGATCTTCTCGGGGGGTCTGAGCCCCAGCCTGCGGCGCGAGGCCTGG
NM_001168222.1      GAGCTGAAGAACCGGATCTTCTCGGGGGGTCTGAGCCCCAGCCTGCGGCGCGAGGCCTGG
NM_024682.2         GAGCTGAAGAACCGGATCTTCTCGGGGGGTCTGAGCCCCAGCCTGCGGCGCGAGGCCTGG
                    ************************************************************

HsCD00584119        AAGTTCCTCCTAGGGTACCTCAGCTGGGAAGGCACAGCTGAGGAGCACAAGGCCCACATA
NM_001168222.1      AAGTTCCTCCTAGGGTACCTCAGCTGGGAAGGCACAGCTGAGGAGCACAAGGCCCACATA
NM_024682.2         AAGTTCCTCCTAGGGTACCTCAGCTGGGAAGGCACAGCTGAGGAGCACAAGGCCCACATA
                    ************************************************************

HsCD00584119        CGCAAGAAAACGGATGAGTATTTCCGCATGAAGCTGCAGTGGAAATCTGTGAGCCCTGAG
NM_001168222.1      CGCAAGAAAACGGATGAGTATTTCCGCATGAAGCTGCAGTGGAAATCTGTGAGCCCTGAG
NM_024682.2         CGCAAGAAAACGGATGAGTATTTCCGCATGAAGCTGCAGTGGAAATCTGTGAGCCCTGAG
                    ************************************************************

HsCD00584119        CAGGAGCGGAGAAACTCACTTCTGCATGGATACCTCAGCCTCATCGAAAGGGATGTGAGC
NM_001168222.1      CAGGAGCGGAGAAACTCACTTCTGCATGGATACCGCAGCCTCATCGAAAGGGATGTGAGC
NM_024682.2         CAGGAGCGGAGAAACTCACTTCTGCATGGATACCGCAGCCTCATCGAAAGGGATGTGAGC
                    ********************************** *************************

HsCD00584119        CGCACTGACAGGACCAACAAGTTCTACGAGGGTCCCGAGAACCCGGGGCTGGGCCTGCTG
NM_001168222.1      CGCACTGACAGGACCAACAAGTTCTACGAGGGTCCCGAGAACCCGGGGCTGGGCCTGCTG
NM_024682.2         CGCACTGACAGGACCAACAAGTTCTACGAGGGTCCCGAGAACCCGGGGCTGGGCCTGCTG
                    ************************************************************

HsCD00584119        AACGATATCCTCCTCACCTACTGCATGTATCACTTCGACCTCGGCTACGTCCAGGGCATG
NM_001168222.1      AACGATATCCTCCTCACCTACTGCATGTATCACTTCGACCTCGGCTACGTCCAGGGCATG
NM_024682.2         AACGATATCCTCCTCACCTACTGCATGTATCACTTCGACCTCGGCTACGTCCAGGGCATG
                    ************************************************************

HsCD00584119        AGTGATCTTCTCTCCCCGATCCTCTACGTCATTCAGAACGAGGTGGATGCTTTCTGGTGT
NM_001168222.1      AGTGATCTTCTCTCCCCGATCCTCTACGTCATTCAGAACGAGGTGGATGCTTTCTGGTGT
NM_024682.2         AGTGATCTTCTCTCCCCGATCCTCTACGTCATTCAGAACGAGGTGGATGCTTTCTGGTGT
                    ************************************************************

HsCD00584119        TTCTGTGGCTTCATGGAGCTCGTGCAAGGGAACTTTGAAGAGAGCCAGGAGACCATGAAG
NM_001168222.1      TTCTGTGGCTTCATGGAGCTCGTGCAAGGGAACTTTGAAGAGAGCCAGGAGACCATGAAG
NM_024682.2         TTCTGTGGCTTCATGGAGCTCGTGCAAGGGAACTTTGAAGAGAGCCAGGAGACCATGAAG
                    ************************************************************

HsCD00584119        CGGCAACTCGGGCGACTGCTGCTGCTCCTGAGGGTGCTGGACCCCCTGCTCTGCGACTTC
NM_001168222.1      CGGCAACTCGGGCGACTGCTGCTGCTCCTGAGGGTGCTGGACCCCCTGCTCTGCGACTTC
NM_024682.2         CGGCAACTCGGGCGACTGCTGCTGCTCCTGAGGGTGCTGGACCCCCTGCTCTGCGACTTC
                    ************************************************************

HsCD00584119        CTGGATTCCCAGGACTCCGGCTCTCTCTGCTTCTGTTTCCGGTGGCTGCTCATCTGGTTC
NM_001168222.1      CTGGATTCCCAGGACTCCGGCTCTCTCTGCTTCTGTTTCCGGTGGCTGCTCATCTGGTTC
NM_024682.2         CTGGATTCCCAGGACTCCGGCTCTCTCTGCTTCTGTTTCCGGTGGCTGCTCATCTGGTTC
                    ************************************************************

HsCD00584119        AAGAGGGAATTCCCCTTCCCGGATGTCCTTCGGCTGTGGGAGGTGCTGTGGACAGGGCTC
NM_001168222.1      AAGAGGGAATTCCCCTTCCCGGATGTCCTTCGGCTGTGGGAGGTGCTGTGGACAGGGCTC
NM_024682.2         AAGAGGGAATTCCCCTTCCCGGATGTCCTTCGGCTGTGGGAGGTGCTGTGGACAGGGCTC
                    ************************************************************

HsCD00584119        CCTGGCCCCAATCTGCACCTGCTGGTGGCCTGCGCCATCCTGGACATGGAGAGGGACACC
NM_001168222.1      CCTGGCCCCAATCTGCACCTGCTGGTGGCCTGCGCCATCCTGGACATGGAGAGGGACACC
NM_024682.2         CCTGGCCCCAATCTGCACCTGCTGGTGGCCTGCGCCATCCTGGACATGGAGAGGGACACC
                    ************************************************************

HsCD00584119        CTCATGCTGTCCGGCTTCGGCTCCAATGAGATCCTCAAGCACATCAACGAGCTGACTATG
NM_001168222.1      CTCATGCTGTCCGGCTTCGGCTCCAATGAGATCCTCAAGCACATCAACGAGCTGACTATG
NM_024682.2         CTCATGCTGTCCGGCTTCGGCTCCAATGAGATCCTCAAGCACATCAACGAGCTGACTATG
                    ************************************************************

HsCD00584119        AAGCTGAGCGTGGAGGACGTGCTGACCCGCGCCGAGGCCCTGCACCGCCAGCTAACCGCC
NM_001168222.1      AAGCTGAGCGTGGAGGACGTGCTGACCCGCGCCGAGGCCCTGCACCGCCAGCTAACCGCC
NM_024682.2         AAGCTGAGCGTGGAGGACGTGCTGACCCGCGCCGAGGCCCTGCACCGCCAGCTAACCGCC
                    ************************************************************

HsCD00584119        TGCCCCGAGCTGCCCCACAACGTGCAGGAGATCCTGGGGCTGGCCCCGCCCGCAGAGTAG
NM_001168222.1      TGCCCCGAGCTGCCCCACAACGTGCAGGAGATCCTGGGGCTGGCCCCGCCCGCAGAGCCC
NM_024682.2         TGCCCCGAGCTGCCCCACAACGTGCAGGAGATCCTGGGGCTGGCCCCGCCCGCAGAGCCC
                    ********************************************************* . 

HsCD00584119        CTCGAGATCCGGCTGCTAACAAAGCCCGAAAG----------------------------
NM_001168222.1      CACAGCCCCTCGCCCACCGCCTCCCCGCTGCCTCTGTCGCCCACCCGGGCCCCGCCCACC
NM_024682.2         CACAGCCCCTCGCCCACCGCCTCCCCGCTGCCTCTGTCGCCCACCCGGGCCCCGCCCACC
                    *:*.. . *  **  . ..*.:. **  :..                             

HsCD00584119        ------------------------------------------------------------
NM_001168222.1      CCGCCGCCCTCCACGGACACAGCCCCGCAGCCCGACAGCAGCCTGGAGATCCTGCCCGAG
NM_024682.2         CCGCCGCCCTCCACGGACACAGCCCCGCAGCCCGACAGCAGCCTGGAGATCCTGCCCGAG
                                                                                

HsCD00584119        ---------------------------
NM_001168222.1      GAGGAGGACGAGGGCGCCGACTCCTAA
NM_024682.2         GAGGAGGACGAGGGCGCCGACTCCTAA