TSC2 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  TSC2
Gene Name:  tuberous sclerosis 2
Original Clone ID:
HR3439F-34-625-15.1
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15_NESG
Format: 
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00584166
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1782nts
Open reading frame: 5 to 1786

CCATATGAAACAGACGGAGTTTATCATCACCGCGGAAATACTGAGAGAACTGAGCATGGA
ATGTGGCCTCAACAATCGCATCCGGATGATAGGGCAGATTTGTGAAGTCGCAAAAACCAA
GAAATTTGAAGAGCACGCAGTGGAAGCACTCTGGAAGGCGGTCGCGGATCTGTTGCAGCC
GGAGCGGCCGCTGGAGGCCCGGCACGCGGTGCTGGCTCTGCTGGAGGCCATCGTGCAGGG
GCAGGGCGAGCGTTTGGGGGTCCTCAGAGCCCTCTTCTTTAAGGTCATCAAGGATTACCC
TTCCAACGAAGACCTTCACGAAAGGCTGGAGGTTTTCAAGGCCCTCACAGACAATGGGAG
ACACATCACCTACTTGGAGGAAGAGCTGGCTGACTTTGTCCTGCAGTGGATGGATGTTGG
CTTGTCCTCGGAATTCCTTCTGGTGCTGGTGAACTTGGTCAAATTCAATAGCTGTTACCT
CGACGAGTACATCGCAAGGATGGTTCAGATGATCTGTCTGCTGTGCGTCCGGACCGCGTC
CTCTGTGGACATAGAGGTCTCCCTGCAGGTGTTGGACGCCGTGGTCTGCTACAACTGCCT
GCCGGCTGAGAGCCTCCCGCTGTTCATCGTTACCCTCTGTCGCACCATCAACGTCAAGGA
GCTCTGCGAGCCTTGCTGGAAGCTGATGCGGAACCTCCTTGGCACCCACCTGGGCCACAG
CGCCATCTACAACATGTGCCACCTCATGGAGGACAGAGCCTACATGGAGGACGCGCCCCT
GCTGAGAGGAGCCGTGTTTTTTGTGGGCATGGCTCTCTGGGGAGCCCACCGGCTCTATTC
TCTCAGGAACTCGCCGACATCTGTGTTGCCATCATTTTACCAGGCCATGGCATGTCCGAA
CGAGGTGGTGTCCTATGAGATCGTCCTGTCCATCACCAGGCTCATCAAGAAGTATAGGAA
GGAGCTCCAGGTGGTGGCGTGGGACATTCTGCTGAACATCATCGAACGGCTCCTTCAGCA
GCTCCAGACCTTGGACAGCCCGGAGCTCAGGACCATCGTCCATGACCTGTTGACCACGGT
GGAGGAGCTGTGTGACCAGAACGAGTTCCACGGGTCTCAGGAGAGATACTTTGAACTGGT
GGAGAGATGTGCGGACCAGAGGCCTGAGTCCTCCCTCCTGAACCTGATCTCCTATAGAGC
GCAGTCCATCCACCCGGCCAAGGACGGCTGGATTCAGAACCTGCAGGCGCTGATGGAGAG
ATTCTTCAGGAGCGAGTCCCGAGGCGCCGTGCGCATCAAGGTGCTGGACGTGCTGTCCTT
TGTGCTGCTCATCAACAGGCAGTCCTATGAGGAGGAGCTGATTAACTCAGTGGTCATCTC
GCAGCTCTCCCACATCCCCGAGGATAAAGACCACCAGGTCCGAAAGCTGGCCACCCAGTT
GCTGGTGGACCTGGCAGAGGGCTGCCACACACACCACTTCAACAGCCTGCTGGACATCAT
CGAGAAGGTGATGGCCCGCTCCCTCTCCCCACCCCCGGAGCTGGAAGAAAGGGATGTGGC
CGCATACTCGGCCTCCTTGGAGGATGTGAAGACAGCCGTCCTGGGGCTTCTGGTCATCCT
TCAGACCAAGCTGTACACCCTGCCTGCAAGCCACGCCACGCGTGTGTATGAGATGCTGGT
CAGCCACATTCAGCTCCACTACAAGCACAGCTACACCCTGCCAATCGCGAGCAGCATCCG
GCTGCAGGCCTTTGACTTCCTGTTGCTGCTGCGGGCCGACTCATAG

Protein sequence predicted based on nucleotide sequence

MKQTEFIITAEILRELSMECGLNNRIRMIGQICEVAKTKKFEEHAVEALWKAVADLLQPE
RPLEARHAVLALLEAIVQGQGERLGVLRALFFKVIKDYPSNEDLHERLEVFKALTDNGRH
ITYLEEELADFVLQWMDVGLSSEFLLVLVNLVKFNSCYLDEYIARMVQMICLLCVRTASS
VDIEVSLQVLDAVVCYNCLPAESLPLFIVTLCRTINVKELCEPCWKLMRNLLGTHLGHSA
IYNMCHLMEDRAYMEDAPLLRGAVFFVGMALWGAHRLYSLRNSPTSVLPSFYQAMACPNE
VVSYEIVLSITRLIKKYRKELQVVAWDILLNIIERLLQQLQTLDSPELRTIVHDLLTTVE
ELCDQNEFHGSQERYFELVERCADQRPESSLLNLISYRAQSIHPAKDGWIQNLQALMERF
FRSESRGAVRIKVLDVLSFVLLINRQSYEEELINSVVISQLSHIPEDKDHQVRKLATQLL
VDLAEGCHTHHFNSLLDIIEKVMARSLSPPPELEERDVAAYSASLEDVKTAVLGLLVILQ
TKLYTLPASHATRVYEMLVSHIQLHYKHSYTLPIASSIRLQAFDFLLLLRADS*

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
a220g,K74E;c568t,L190L;

t1340c,F447S
                                                    

5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
SolubleProtein: Not_Tested
ProteinExpressed: Tested_Not_Found
Find experimental protocols in HR3439F
Recommended expression in:
Not Applicable