DNASU Plasmid | RASSF2 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: RASSF2

Gene Name: Ras association (RalGDS/AF-6) domain family member 2

Original Clone ID:

HR2904-9-326-15.7

Keyword:

full length protein truncation

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00584324

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 960nts

Open reading frame: 4 to 963

Reference Sequence Alignment

CATATGCTAGTCCCATGTGGACAAGATAAATACATTTCCAAAAATGAACTTCTCTTGCAT
CTGAAGACCTACAACTTGTACTATGAAGGCCAGAATTTACAGCTCCGGCACCGGGAGGAA
GAAGACGAGTTCATTGTGGAGGGGCTCCTGAACATCTCCTGGGGCCTGCGCCGGCCCATT
CGCCTGCAGATGCAGGATGACAACGAACGCATTCGACCCCCTCCATCCTCCTCCTCCTGG
CACTCTGGCTGTAACCTGGGGGCTCAGGGAGCCACTCTGAAGCCCCTGACTGTGCCCAAA
GTTCAGATCTCAGAGGTGGATGCCCCGCCGGAGGGTGACCAGATGCCAAGCTCCACAGAC
TCCAGGGGCCTGAAGCCCCTGCAGGAGGACACCCCACAGCTGATGCGCACACGCAGTGAT
GTTGGGGTGCGTCGCCGTGGCAATGTGAGGACGCCTAGTGACCAGCGGCGAATCAGACGC
CACCGCTTCTCCATCAACGCCCATTTCTACAACCATAAGACATCCGTGTTCACACCAGCC
TATGGCTCTGTCACCAACGTCCGCATCAACAGCACCATGTCCACCCCACAGGTCCTGAAG
CTGCTGCTCAACAAATTTAAGATTGAGAATTCAGCAGAGGAGTTTGCCTTGTACGTGGTC
CATACGAGTGGTGAGAAACAGAAGCTGAAGGCCACCGATTACCCGCTGATTGCCCGAATC
CTCCAGGGCCCATATGAGCAGATCTCCAAAGTGTTCCTAATGGAGAAGGACCAGGTGGAG
GAAGTCACCTACGACGTGGCCCAGTATATAAAGTTCGAGATGCCGGTACTTAAAAGCTTC
ATTCAGAAGCTCCAGGAGGAAGAAGATCGGGAAGTAAAGAAGCTGATGCGCAAGTACACC
GTGCTCCGGCTAATGATTCGACAGAGGCTGGAGGAGATAGCCGAGACCCCAGCAACAATC
TAG

Protein sequence predicted based on nucleotide sequence

MLVPCGQDKYISKNELLLHLKTYNLYYEGQNLQLRHREEEDEFIVEGLLNISWGLRRPIR
LQMQDDNERIRPPPSSSSWHSGCNLGAQGATLKPLTVPKVQISEVDAPPEGDQMPSSTDS
RGLKPLQEDTPQLMRTRSDVGVRRRGNVRTPSDQRRIRRHRFSINAHFYNHKTSVFTPAY
GSVTNVRINSTMSTPQVLKLLLNKFKIENSAEEFALYVVHTSGEKQKLKATDYPLIARIL
QGPYEQISKVFLMEKDQVEEVTYDVAQYIKFEMPVLKSFIQKLQEEEDREVKKLMRKYTV
LRLMIRQRLEEIAETPATI*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

a268g,T90A;g497c,G166A;

a577t,T193S;g731a,C244Y

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR2904

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

RASSF2

Symbol Nomenclature :

RASSF2

SYNONYM :

CENP-34; RASFADIN

Designation :

Ras association (RalGDS/AF-6) domain family 2|centromere protein 34|ras association domain-containing protein 2

Full Nomenclature :

Ras association (RalGDS/AF-6) domain family member 2

GENEID :

9770

Locus Tag :

RP4-599I11.1

GI :

25777677

GenBank Accession :

NM_170774

HGNC :

9883

MIM :

609492

Vega :

OTTHUMG00000031790

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00584324      ------------------CATATGCTAGTCCCATGTGGACAAGATAAATACATTTCCAAA
NM_014737.2       ATGGACTACAGCCACCAAACGTCCCTAGTCCCATGTGGACAAGATAAATACATTTCCAAA
NM_170774.1       ATGGACTACAGCCACCAAACGTCCCTAGTCCCATGTGGACAAGATAAATACATTTCCAAA
                                    .. :  ************************************

HsCD00584324      AATGAACTTCTCTTGCATCTGAAGACCTACAACTTGTACTATGAAGGCCAGAATTTACAG
NM_014737.2       AATGAACTTCTCTTGCATCTGAAGACCTACAACTTGTACTATGAAGGCCAGAATTTACAG
NM_170774.1       AATGAACTTCTCTTGCATCTGAAGACCTACAACTTGTACTATGAAGGCCAGAATTTACAG
                  ************************************************************

HsCD00584324      CTCCGGCACCGGGAGGAAGAAGACGAGTTCATTGTGGAGGGGCTCCTGAACATCTCCTGG
NM_014737.2       CTCCGGCACCGGGAGGAAGAAGACGAGTTCATTGTGGAGGGGCTCCTGAACATCTCCTGG
NM_170774.1       CTCCGGCACCGGGAGGAAGAAGACGAGTTCATTGTGGAGGGGCTCCTGAACATCTCCTGG
                  ************************************************************

HsCD00584324      GGCCTGCGCCGGCCCATTCGCCTGCAGATGCAGGATGACAACGAACGCATTCGACCCCCT
NM_014737.2       GGCCTGCGCCGGCCCATTCGCCTGCAGATGCAGGATGACAACGAACGCATTCGACCCCCT
NM_170774.1       GGCCTGCGCCGGCCCATTCGCCTGCAGATGCAGGATGACAACGAACGCATTCGACCCCCT
                  ************************************************************

HsCD00584324      CCATCCTCCTCCTCCTGGCACTCTGGCTGTAACCTGGGGGCTCAGGGAGCCACTCTGAAG
NM_014737.2       CCATCCTCCTCCTCCTGGCACTCTGGCTGTAACCTGGGGGCTCAGGGAACCACTCTGAAG
NM_170774.1       CCATCCTCCTCCTCCTGGCACTCTGGCTGTAACCTGGGGGCTCAGGGAACCACTCTGAAG
                  ************************************************.***********

HsCD00584324      CCCCTGACTGTGCCCAAAGTTCAGATCTCAGAGGTGGATGCCCCGCCGGAGGGTGACCAG
NM_014737.2       CCCCTGACTGTGCCCAAAGTTCAGATCTCAGAGGTGGATGCCCCGCCGGAGGGTGACCAG
NM_170774.1       CCCCTGACTGTGCCCAAAGTTCAGATCTCAGAGGTGGATGCCCCGCCGGAGGGTGACCAG
                  ************************************************************

HsCD00584324      ATGCCAAGCTCCACAGACTCCAGGGGCCTGAAGCCCCTGCAGGAGGACACCCCACAGCTG
NM_014737.2       ATGCCAAGCTCCACAGACTCCAGGGGCCTGAAGCCCCTGCAGGAGGACACCCCACAGCTG
NM_170774.1       ATGCCAAGCTCCACAGACTCCAGGGGCCTGAAGCCCCTGCAGGAGGACACCCCACAGCTG
                  ************************************************************

HsCD00584324      ATGCGCACACGCAGTGATGTTGGGGTGCGTCGCCGTGGCAATGTGAGGACGCCTAGTGAC
NM_014737.2       ATGCGCACACGCAGTGATGTTGGGGTGCGTCGCCGTGGCAATGTGAGGACGCCTAGTGAC
NM_170774.1       ATGCGCACACGCAGTGATGTTGGGGTGCGTCGCCGTGGCAATGTGAGGACGCCTAGTGAC
                  ************************************************************

HsCD00584324      CAGCGGCGAATCAGACGCCACCGCTTCTCCATCAACGCCCATTTCTACAACCATAAGACA
NM_014737.2       CAGCGGCGAATCAGACGCCACCGCTTCTCCATCAACGGCCATTTCTACAACCATAAGACA
NM_170774.1       CAGCGGCGAATCAGACGCCACCGCTTCTCCATCAACGGCCATTTCTACAACCATAAGACA
                  ************************************* **********************

HsCD00584324      TCCGTGTTCACACCAGCCTATGGCTCTGTCACCAACGTCCGCATCAACAGCACCATGTCC
NM_014737.2       TCCGTGTTCACACCAGCCTATGGCTCTGTCACCAACGTCCGCATCAACAGCACCATGACC
NM_170774.1       TCCGTGTTCACACCAGCCTATGGCTCTGTCACCAACGTCCGCATCAACAGCACCATGACC
                  *********************************************************:**

HsCD00584324      ACCCCACAGGTCCTGAAGCTGCTGCTCAACAAATTTAAGATTGAGAATTCAGCAGAGGAG
NM_014737.2       ACCCCACAGGTCCTGAAGCTGCTGCTCAACAAATTTAAGATTGAGAATTCAGCAGAGGAG
NM_170774.1       ACCCCACAGGTCCTGAAGCTGCTGCTCAACAAATTTAAGATTGAGAATTCAGCAGAGGAG
                  ************************************************************

HsCD00584324      TTTGCCTTGTACGTGGTCCATACGAGTGGTGAGAAACAGAAGCTGAAGGCCACCGATTAC
NM_014737.2       TTTGCCTTGTACGTGGTCCATACGAGTGGTGAGAAACAGAAGCTGAAGGCCACCGATTAC
NM_170774.1       TTTGCCTTGTACGTGGTCCATACGAGTGGTGAGAAACAGAAGCTGAAGGCCACCGATTAC
                  ************************************************************

HsCD00584324      CCGCTGATTGCCCGAATCCTCCAGGGCCCATATGAGCAGATCTCCAAAGTGTTCCTAATG
NM_014737.2       CCGCTGATTGCCCGAATCCTCCAGGGCCCATGTGAGCAGATCTCCAAAGTGTTCCTAATG
NM_170774.1       CCGCTGATTGCCCGAATCCTCCAGGGCCCATGTGAGCAGATCTCCAAAGTGTTCCTAATG
                  *******************************.****************************

HsCD00584324      GAGAAGGACCAGGTGGAGGAAGTCACCTACGACGTGGCCCAGTATATAAAGTTCGAGATG
NM_014737.2       GAGAAGGACCAGGTGGAGGAAGTCACCTACGACGTGGCCCAGTATATAAAGTTCGAGATG
NM_170774.1       GAGAAGGACCAGGTGGAGGAAGTCACCTACGACGTGGCCCAGTATATAAAGTTCGAGATG
                  ************************************************************

HsCD00584324      CCGGTACTTAAAAGCTTCATTCAGAAGCTCCAGGAGGAAGAAGATCGGGAAGTAAAGAAG
NM_014737.2       CCGGTACTTAAAAGCTTCATTCAGAAGCTCCAGGAGGAAGAAGATCGGGAAGTAAAGAAG
NM_170774.1       CCGGTACTTAAAAGCTTCATTCAGAAGCTCCAGGAGGAAGAAGATCGGGAAGTAAAGAAG
                  ************************************************************

HsCD00584324      CTGATGCGCAAGTACACCGTGCTCCGGCTAATGATTCGACAGAGGCTGGAGGAGATAGCC
NM_014737.2       CTGATGCGCAAGTACACCGTGCTCCGGCTAATGATTCGACAGAGGCTGGAGGAGATAGCC
NM_170774.1       CTGATGCGCAAGTACACCGTGCTCCGGCTAATGATTCGACAGAGGCTGGAGGAGATAGCC
                  ************************************************************

HsCD00584324      GAGACCCCAGCAACAATCTAG
NM_014737.2       GAGACCCCAGCAACAATCTGA
NM_170774.1       GAGACCCCAGCAACAATCTGA
                  *******************..