Gene:
Gene Symbol:
GCN5
Gene Name:
Gcn5p
Original Clone ID:
YR337-64-439-Av6HT.5
Keyword:
None
Species:
Saccharomyces cerevisiae
Type:
cDNA
Vector Name:
pET15TEV_AVI_NESG
Format:
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
Made unavailable due to incorrect and/or unavailable vector information
Mutation / Discrepancy:
No / Yes
Publications:
None
Authors:
PSI:Biology
Northeast Structural Genomics Consortium
Northeast Structural Genomics Consortium
ScCD00584475
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UNAVAILABLE
No restriction
Special MTA: PSI/NESG
Special MTA: PSI/NESG
Insert sequence: 1134nts
Open reading frame: 51 to 1184
GGCACGAACATCACCATCACCATCACGAAAACCTGTATTTTCAGAGCCATATGTCTGAAG
TGGTTACAGATGTGGAAAAAGGAATTGTCAAATTTGAATTTGATGGTGTTGAATACACAT
TCAAAGAGAGACCCAGTGTCGTAGAGGAAAATGAAGGTAAAATTGAGTTTAGGGTGGTGA
ATAATGATAATACTAAAAAAAACATGATGGTCCTAACTGGATTAAAAAACATTTTTCAAA
AGCAATTACCAAAAATGCCCAAAGAATACATTGCCAGGTTAGTCTATGATCGAAGTCATC
TTTCCATGGCTGTCATTAGGAAGCCATTGACTGTCGTAGGTGGCATAACATATCGACCTT
TCGATAAGAGAGAATTCGCAGAAATTGTTTTCTGTGCCATCAGTTCGACGGAACAGGTAC
GCGGTTATGGTGCGCATCTAATGAATCACTTAAAAGACTATGTTAGAAATACCTCGAACA
TAAAATATTTTTTGACATATGCAGATAATTACGCTATTGGATACTTTAAAAAGCAAGGCT
TCACTAAAGAAATCACGTTGGATAAAAGTATATGGATGGGATATATTAAAGATTATGAAG
GTGGTACGCTGATGCAATGTTCTATGTTACCAAGAATACGATATTTGGACGCAGGTAAGA
TTCTATTATTACAAGAAGCGGCCCTGCGAAGAAAAATAAGAACGATTTCGAAATCGCATA
TTGTAAGGCCTGGTTTAGAGCAATTCAAAGACTTAAACAATATCAAACCGATTGATCCAA
TGACTATTCCTGGCTTGAAAGAAGCCGGCTGGACTCCCGAGATGGATGCGTTGGCACAAC
GTCCCAAGCGTGGTCCACACGATGCAGCAATACAGAATATACTCACAGAGCTACAAAATC
ATGCAGCAGCTTGGCCCTTCTTACAACCCGTTAATAAAGAGGAGGTCCCCGACTATTATG
ATTTTATCAAAGAGCCAATGGACTTGAGCACCATGGAAATAAAATTAGAGAGCAACAAAT
ATCAGAAGATGGAAGACTTCATATATGATGCCAGATTGGTGTTTAACAATTGCCGAATGT
ACAATGGCGAGAATACGTCGTATTACAAGTATGCTAATAGGCTAGAGAAATTCTTCAATA
ATAAAGTAAAAGAAATACCTGAATATTCTCACCTTATTGATTAG
Protein sequence predicted based on nucleotide sequence
MSEVVTDVEKGIVKFEFDGVEYTFKERPSVVEENEGKIEFRVVNNDNTKKNMMVLTGLKN IFQKQLPKMPKEYIARLVYDRSHLSMAVIRKPLTVVGGITYRPFDKREFAEIVFCAISST EQVRGYGAHLMNHLKDYVRNTSNIKYFLTYADNYAIGYFKKQGFTKEITLDKSIWMGYIK DYEGGTLMQCSMLPRIRYLDAGKILLLQEAALRRKIRTISKSHIVRPGLEQFKDLNNIKP IDPMTIPGLKEAGWTPEMDALAQRPKRGPHDAAIQNILTELQNHAAAWPFLQPVNKEEVP DYYDFIKEPMDLSTMEIKLESNKYQKMEDFIYDARLVFNNCRMYNGENTSYYKYANRLEK FFNNKVKEIPEYSHLID*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
g148a,E50K
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinPurified: Not_Tested
ProteinExpressed: Protein_Confirmed
SolubleProtein: Tested_Not_Soluble
Find experimental protocols in YR337
Recommended expression in:
Not Applicable
Vector Name:
pET15TEV_AVI_NESG
Synonyms:
''
Sequencing Primer:
Forward:
Unknown
Reverse:
Unknown
Description:
Bacterial expression vector; ampicillin resistance in bacteria
Comments:
None
Size (bp):
0
Parent Vector:
None
Properties:
''
Author Name:
Northeast Structural Genomics Consortium
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Not Available
Vector Features:
Type
Name
Description
Start
End
selectable marker
AmpR
0
0
