DNASU Plasmid | MCM5 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: MCM5

Gene Name: minichromosome maintenance complex component 5

Original Clone ID:

HR3328-1-574-15.18

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / Yes

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00584992

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 1725nts

Open reading frame: 51 to 1775

Reference Sequence Alignment

ACTTTAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGTCGGGAT
TCGACGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGTGACGCCCAGACCGACGAGG
GGCAGGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAG
TGGGCACCGACCGCACGGGCTTCACCTTCAAATACAGGGACGAACTCAAGCGGCATTACA
ACCTGGGGGAGTACTGGATTGAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACC
TGGCCGACTACTTGTACAAGCAGCCAGCCGAGCACCTGCAGCTGCTGGAGGAAGCTGCCA
AGGAGGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGGGGAGGAGGTGCTCCAGGACA
TCCAGGTCATGCTCAAGCCGGACGCCAGCCCTTCCAGCATTCGTAGCCTGAAGTCGGACA
TGATGTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCA
AGGCCACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGCCA
TGCGCCCTGGCCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGATCAGGCTGGGC
GCCCCAAATGCCCATTGGACCCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACT
TCCAGACCCTGAAGCTGCAGGAGCTGCCTGATGCAGTCCCCCACGGGGAGATGCCCAGAC
ACATGCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGTCGTCCCTGGGAACAGGGTTA
CCATCATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACTACCAGCAGGGGCCGTGACA
GGGTGGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAGGTGGACACAG
ATGGCTCTGACCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAGGAGTTCCGTC
GCCTGGCTGCCCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCCCCCTCCATCT
TTGGGGGCACAGACATGAAGAAGGCCATTGCCTGCCTGCTCTTTGGGGGCTCCCGAAAGA
GGCTCCCTGATGGACTTACTCGCCGAGGAGACATCAACCTGCTGATGCTAGGGGACCCTG
GGACAGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTGTTCTCCCATTGGGGTATACA
CGTCTGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGGGACCCTTCGT
CCCGGAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGGGTCGTCTGTA
TTGACGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAAGCCATGGAGC
AGCAGACCATCTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCCCGCTGCTCCG
TCCTGGCTGCTGCCAACTCAGTGTTCGGCCGCCGGGATGAGACGAAGGGGGAGGACAACA
TTGACTTCATGCCCACCATCTTGTCGCGCTTCGACATGATCTTCATCGTCAAGGATGAGC
ACAATGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCATCACTCTGCACGTGAGCGCAC
TGACACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAGAAGTTTATTG
CCTACTGCCGAGTGAAGTGTGGCCCCCGGCTGTAG

Protein sequence predicted based on nucleotide sequence

MSGFDDPGIFYSDSFGGDAQTDEGQARKSQLQRRFKEFLRQYRVGTDRTGFTFKYRDELK
RHYNLGEYWIEVEMEDLASFDEDLADYLYKQPAEHLQLLEEAAKEVADEVTRPRPSGEEV
LQDIQVMLKPDASPSSIRSLKSDMMSHLVKIPGIIIAASAVRAKATRISIQCRSCRNTLT
NIAMRPGLEGYALPRKCNTDQAGRPKCPLDPYFIMPDKCKCVDFQTLKLQELPDAVPHGE
MPRHMQLYCDRYLCDKVVPGNRVTIMGIYSIKKFGLTTSRGRDRVGVGIRSSYIRVLGIQ
VDTDGSDRSFAGAVSPQEEEEFRRLAALPNVYEVISKSIAPSIFGGTDMKKAIACLLFGG
SRKRLPDGLTRRGDINLLMLGDPGTAKSQLLKFVEKCSPIGVYTSGKGSSAAGLTASVMR
DPSSRNFIMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNS
RCSVLAAANSVFGRRDETKGEDNIDFMPTILSRFDMIFIVKDEHNEERDVMLAKHVITLH
VSALTQTQAVEGEIDLAKLKKFIAYCRVKCGPRL*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

c51t,G17G;g61a,A21T;

t171c,D57D;t388c,S130P;

g920a,G307D;t1483c,W495R

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

ProteinExpressed: Protein_Confirmed

SolubleProtein: Tested_Not_Soluble

Find experimental protocols in HR3328

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

MCM5

Symbol Nomenclature :

MCM5

SYNONYM :

CDC46; P1-CDC46

Designation :

CDC46 homolog|DNA replication licensing factor MCM5|MCM5 minichromosome maintenance deficient 5, cell division cycle 46|minichromosome maintenance deficient 5 (cell division cycle 46)

Full Nomenclature :

minichromosome maintenance complex component 5

GENEID :

4174

Locus Tag :

RP5-824I19__A.1

GI :

23510448

GenBank Accession :

NM_006739

HGNC :

6948

MIM :

602696

Vega :

OTTHUMG00000150961

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00584992      ACTTTAGAAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGTCGGGAT
NM_006739.3       --------------------------------------------------ATGTCGGGAT
                                                                    **********

HsCD00584992      TCGACGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGTGACGCCCAGACCGACGAGG
NM_006739.3       TCGACGATCCTGGCATTTTCTACAGCGACAGCTTCGGGGGCGACGCCCAGGCCGACGAGG
                  **************************************** *********.*********

HsCD00584992      GGCAGGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAG
NM_006739.3       GGCAGGCCCGCAAATCGCAGCTGCAGAGGCGCTTCAAGGAGTTCCTGCGGCAGTACCGAG
                  ************************************************************

HsCD00584992      TGGGCACCGACCGCACGGGCTTCACCTTCAAATACAGGGACGAACTCAAGCGGCATTACA
NM_006739.3       TGGGCACCGACCGCACGGGCTTCACCTTCAAATACAGGGATGAACTCAAGCGGCATTACA
                  **************************************** *******************

HsCD00584992      ACCTGGGGGAGTACTGGATTGAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACC
NM_006739.3       ACCTGGGGGAGTACTGGATTGAGGTGGAGATGGAGGATCTGGCCAGCTTTGATGAGGACC
                  ************************************************************

HsCD00584992      TGGCCGACTACTTGTACAAGCAGCCAGCCGAGCACCTGCAGCTGCTGGAGGAAGCTGCCA
NM_006739.3       TGGCCGACTACTTGTACAAGCAGCCAGCCGAGCACCTGCAGCTGCTGGAGGAAGCTGCCA
                  ************************************************************

HsCD00584992      AGGAGGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGGGGAGGAGGTGCTCCAGGACA
NM_006739.3       AGGAGGTAGCTGATGAGGTGACCCGGCCCCGGCCTTCTGGGGAGGAGGTGCTCCAGGACA
                  ************************************************************

HsCD00584992      TCCAGGTCATGCTCAAGCCGGACGCCAGCCCTTCCAGCATTCGTAGCCTGAAGTCGGACA
NM_006739.3       TCCAGGTCATGCTCAAGTCGGACGCCAGCCCTTCCAGCATTCGTAGCCTGAAGTCGGACA
                  ***************** ******************************************

HsCD00584992      TGATGTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCA
NM_006739.3       TGATGTCACACCTGGTGAAGATCCCTGGCATCATCATCGCGGCCTCTGCGGTCCGTGCCA
                  ************************************************************

HsCD00584992      AGGCCACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGCCA
NM_006739.3       AGGCCACCCGCATCTCTATCCAGTGCCGCAGCTGCCGCAACACCCTCACCAACATTGCCA
                  ************************************************************

HsCD00584992      TGCGCCCTGGCCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGATCAGGCTGGGC
NM_006739.3       TGCGCCCTGGCCTCGAGGGCTATGCCCTGCCCAGGAAGTGCAACACAGATCAGGCTGGGC
                  ************************************************************

HsCD00584992      GCCCCAAATGCCCATTGGACCCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACT
NM_006739.3       GCCCCAAATGCCCATTGGACCCGTACTTCATCATGCCCGACAAATGCAAATGCGTGGACT
                  ************************************************************

HsCD00584992      TCCAGACCCTGAAGCTGCAGGAGCTGCCTGATGCAGTCCCCCACGGGGAGATGCCCAGAC
NM_006739.3       TCCAGACCCTGAAGCTGCAGGAGCTGCCTGATGCAGTCCCCCACGGGGAGATGCCCAGAC
                  ************************************************************

HsCD00584992      ACATGCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGTCGTCCCTGGGAACAGGGTTA
NM_006739.3       ACATGCAGCTCTACTGCGACAGGTACCTGTGTGACAAGGTCGTCCCTGGGAACAGGGTTA
                  ************************************************************

HsCD00584992      CCATCATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACTACCAGCAGGGGCCGTGACA
NM_006739.3       CCATCATGGGCATCTACTCCATCAAGAAGTTTGGCCTGACTACCAGCAGGGGCCGTGACA
                  ************************************************************

HsCD00584992      GGGTGGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAGGTGGACACAG
NM_006739.3       GGGTGGGCGTGGGCATCCGAAGCTCCTACATCCGTGTCCTGGGCATCCAGGTGGACACAG
                  ************************************************************

HsCD00584992      ATGGCTCTGACCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAGGAGTTCCGTC
NM_006739.3       ATGGCTCTGGCCGCAGCTTTGCTGGGGCCGTGAGCCCCCAGGAGGAGGAGGAGTTCCGTC
                  *********.**************************************************

HsCD00584992      GCCTGGCTGCCCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCCCCCTCCATCT
NM_006739.3       GCCTGGCTGCCCTCCCAAATGTCTATGAGGTCATCTCCAAGAGCATCGCCCCCTCCATCT
                  ************************************************************

HsCD00584992      TTGGGGGCACAGACATGAAGAAGGCCATTGCCTGCCTGCTCTTTGGGGGCTCCCGAAAGA
NM_006739.3       TTGGGGGCACAGACATGAAGAAGGCCATTGCCTGCCTGCTCTTTGGGGGCTCCCGAAAGA
                  ************************************************************

HsCD00584992      GGCTCCCTGATGGACTTACTCGCCGAGGAGACATCAACCTGCTGATGCTAGGGGACCCTG
NM_006739.3       GGCTCCCTGATGGACTTACTCGCCGAGGAGACATCAACCTGCTGATGCTAGGGGACCCTG
                  ************************************************************

HsCD00584992      GGACAGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTGTTCTCCCATTGGGGTATACA
NM_006739.3       GGACAGCCAAGTCCCAGCTTCTGAAGTTTGTGGAGAAGTGTTCTCCCATTGGGGTATACA
                  ************************************************************

HsCD00584992      CGTCTGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGGGACCCTTCGT
NM_006739.3       CGTCTGGGAAAGGCAGCAGCGCAGCTGGACTGACAGCCTCGGTGATGAGGGACCCTTCGT
                  ************************************************************

HsCD00584992      CCCGGAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGGGTCGTCTGTA
NM_006739.3       CCCGGAATTTCATCATGGAGGGCGGAGCCATGGTCCTGGCCGATGGTGGGGTCGTCTGTA
                  ************************************************************

HsCD00584992      TTGACGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAAGCCATGGAGC
NM_006739.3       TTGACGAGTTTGACAAGATGCGAGAAGATGACCGTGTGGCAATCCACGAAGCCATGGAGC
                  ************************************************************

HsCD00584992      AGCAGACCATCTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCCCGCTGCTCCG
NM_006739.3       AGCAGACCATCTCTATCGCCAAGGCTGGGATCACCACCACCCTGAACTCCCGCTGCTCCG
                  ************************************************************

HsCD00584992      TCCTGGCTGCTGCCAACTCAGTGTTCGGCCGCCGGGATGAGACGAAGGGGGAGGACAACA
NM_006739.3       TCCTGGCTGCTGCCAACTCAGTGTTCGGCCGCTGGGATGAGACGAAGGGGGAGGACAACA
                  ******************************** ***************************

HsCD00584992      TTGACTTCATGCCCACCATCTTGTCGCGCTTCGACATGATCTTCATCGTCAAGGATGAGC
NM_006739.3       TTGACTTCATGCCCACCATCTTGTCGCGCTTCGACATGATCTTCATCGTCAAGGATGAGC
                  ************************************************************

HsCD00584992      ACAATGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCATCACTCTGCACGTGAGCGCAC
NM_006739.3       ACAATGAGGAGAGGGATGTGATGCTGGCCAAGCATGTCATCACTCTGCACGTGAGCGCAC
                  ************************************************************

HsCD00584992      TGACACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAGAAGTTTATTG
NM_006739.3       TGACACAGACACAGGCTGTGGAGGGCGAGATTGACCTGGCCAAGCTGAAGAAGTTTATTG
                  ************************************************************

HsCD00584992      CCTACTGCCGAGTGAAGTGTGGCCCCCGGCTGTAG-------------------------
NM_006739.3       CCTACTGCCGAGTGAAGTGTGGCCCCCGGCTGTCAGCAGAGGCTGCAGAGAAACTGAAGA
                  *********************************..                         

HsCD00584992      ------------------------------------------------------------
NM_006739.3       ACCGCTACATCATCATGCGGAGCGGGGCCCGTCAGCACGAGAGGGACAGTGACCGCCGCT
                                                                              

HsCD00584992      ------------------------------------------------------------
NM_006739.3       CCAGCATCCCCATCACTGTGCGGCAGCTGGAGGCCATTGTGCGCATCGCGGAAGCCCTCA
                                                                              

HsCD00584992      ------------------------------------------------------------
NM_006739.3       GCAAGATGAAGCTGCAGCCCTTCGCCACAGAGGCAGATGTGGAGGAGGCCCTGCGGCTCT
                                                                              

HsCD00584992      ------------------------------------------------------------
NM_006739.3       TCCAAGTGTCCACGTTGGATGCTGCCTTGTCCGGTACCCTGTCAGGGGTGGAGGGCTTCA
                                                                              

HsCD00584992      ------------------------------------------------------------
NM_006739.3       CCAGCCAGGAGGACCAGGAGATGCTGAGCCGCATCGAGAAGCAGCTCAAGCGCCGCTTTG
                                                                              

HsCD00584992      ------------------------------------------------------------
NM_006739.3       CCATTGGCTCCCAGGTGTCTGAGCACAGCATCATCAAGGACTTCACCAAGCAGAAATACC
                                                                              

HsCD00584992      ------------------------------------------------------------
NM_006739.3       CGGAGCACGCCATCCACAAGGTGCTGCAGCTCATGCTGCGGCGCGGCGAGATCCAGCATC
                                                                              

HsCD00584992      -----------------------------------
NM_006739.3       GCATGCAGCGCAAGGTTCTCTACCGCCTCAAGTGA

Pathways

Reactome :

Activation of ATR in response to replication stress

Reactome :

Activation of the pre-replicative complex

Reactome :

Assembly of the pre-replicative complex

Reactome :

Cell Cycle

Reactome :

Cell Cycle Checkpoints

Reactome :

Cell Cycle, Mitotic

Reactome :

DNA Replication

Reactome :

DNA Replication Pre-Initiation

Reactome :

DNA strand elongation

Reactome :

G1/S Transition

Reactome :

G2/M Checkpoints

Reactome :

M/G1 Transition

Reactome :

Mitotic G1-G1/S phases

Reactome :

Mitotic M-M/G1 phases

Reactome :

Orc1 removal from chromatin

Reactome :

Regulation of DNA replication

Reactome :

Removal of licensing factors from origins

Reactome :

S Phase

Reactome :

Switching of origins to a post-replicative state

Reactome :

Synthesis of DNA

Reactome :

Unwinding of DNA

WikiPathway :

Cell cycle

WikiPathway :

DNA Replication

WikiPathway :

G1 to S cell cycle control

Protein

SMART domain :

MCM : minichromosome maintenance proteins

Structural Annotation Wiki :

HR3328

UniProt :

P33992

HPRD :

04075

Clone

Cloning Information :

626724

Original Clone ID :

HR3328-1-574-15.18

Experimental

TargetTrack - Experimental data :

HR3328

Organism

Species Specific ID:

4174

Genome

Chromosome :

Map Location :

22q13.1

Ensembl :

ENSG00000100297

Reagents

Labome :

MCM5-antibody

MCM5 (H. sapiens )In pET15_NESG (His-tagged bacterial expression vector)

Coding Sequence Details

Recommended Growth Condition:

Vector Features:

Gene

Reference Sequence Alignment

Pathways

Protein

Clone

Experimental

Organism

Genome

Reagents

MCM5 (H. sapiens )
In pET15_NESG (His-tagged bacterial expression vector)