DNASU Plasmid | MAPK8IP1 (H. sapiens )<br>In pET15_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:

Gene Symbol: MAPK8IP1

Gene Name: mitogen-activated protein kinase 8 interacting protein 1

Original Clone ID:

HR4648B-454-705-14.54

Keyword:

targeted domain

Species:

Homo sapiens

Type:

cDNA

Vector Name:

pET15_NESG

Format:

CLOSED

Source:

Northeast Structural Genomics Consortium

Description:

None

Comments:

None

Mutation / Discrepancy:

No / No

Publications:

None

Authors:

PSI:Biology
Northeast Structural Genomics Consortium

HsCD00585251

Price:

AVAILABLE

No restriction
Special MTA: PSI/NESG

Insert Sequence
Protein Sequence

Insert sequence: 762nts

Open reading frame: 44 to 805

Reference Sequence Alignment

AAGGAGATATACCATGGGCCATCACCATCACCATCACAGCCATATGGTCCATTTCTCCAA
GAAATTCCTGAACGTCTTCATGAGTGGCCGCTCCCGCTCCTCCAGTGCTGAGTCCTTCGG
GCTGTTCTCCTGCATCATCAACGGGGAGGAGCAGGAGCAGACCCACCGGGCCATATTCAG
GTTTGTGCCTCGACACGAAGACGAACTTGAGCTGGAAGTGGATGACCCTCTGCTAGTGGA
GCTCCAGGCTGAAGACTACTGGTACGAGGCCTACAACATGCGCACTGGTGCCCGGGGTGT
CTTTCCTGCCTATTACGCCATCGAGGTCACCAAGGAGCCCGAGCACATGGCAGCCCTGGC
CAAAAACAGTGACTGGGTGGACCAGTTCCGGGTGAAGTTCCTGGGCTCAGTCCAGGTTCC
CTATCACAAGGGCAATGACGTCCTCTGTGCTGCTATGCAAAAGATTGCCACCACCCGCCG
GCTCACCGTGCACTTTAACCCGCCCTCCAGCTGTGTCCTGGAGATCAGCGTGCGGGGTGT
GAAGATAGGCGTCAAGGCCGATGACTCCCAGGAGGCCAAGGGGAATAAATGTAGCCACTT
TTTCCAGTTAAAAAACATCTCTTTCTGCGGATATCATCCAAAGAACAACAAGTACTTTGG
GTTCATCACCAAGCACCCCGCCGACCACCGGTTTGCCTGCCACGTCTTTGTGTCTGAAGA
CTCCACCAAAGCCCTGGCAGAGTCCGTGGGGAGAGCATTCCAGCAGTTCTACAAGCAGTT
TGTGGAGTACACCTGCCCCACATAG

Protein sequence predicted based on nucleotide sequence

MVHFSKKFLNVFMSGRSRSSSAESFGLFSCIINGEEQEQTHRAIFRFVPRHEDELELEVD
DPLLVELQAEDYWYEAYNMRTGARGVFPAYYAIEVTKEPEHMAALAKNSDWVDQFRVKFL
GSVQVPYHKGNDVLCAAMQKIATTRRLTVHFNPPSSCVLEISVRGVKIGVKADDSQEAKG
NKCSHFFQLKNISFCGYHPKNNKYFGFITKHPADHRFACHVFVSEDSTKALAESVGRAFQ
QFYKQFVEYTCPT*

Coding Sequence Details

Insert Sequence Verified?:

Verification Method:

Sequence Verification

Mutations:

None

Discrepancy:

None

5' Linker Sequence:

None

3' Linker Sequence:

None

Recommended Growth Condition:

Distributed in bacterial strain:

DH5-alpha T1 phage resistant

Antibiotic Selection:

Host Type: bacterial Marker: ampicillin

Bacterial Selection Condition: 100 ug/mL ampicillin

Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.

Comments: Commonly used conditions for ampicillin resistant plasmid clones.

Protein Expression Results:

ProteinPurified: Not_Tested

SolubleProtein: Not_Tested

ProteinExpressed: Tested_Not_Found

Find experimental protocols in HR4648B

Recommended expression in:

Not Applicable

Vector Name:

pET15_NESG

Synonyms:

pET15, pET15-modified, pET15Cc, pET15_NESG

Sequencing Primer:

Forward: T7

Reverse: T7 terminator

Description:

Bacterial (E. coli) expression vector with T7 lac promoter, adds N-terminal 6x His tag; amp resistance; restriction enzyme cloning.

Comments:

Modified Novagen pET vector-common MCS

Size (bp):

5723

Parent Vector:

None

Properties:

bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker

Author Name:

Gaetano T. Montleione
Jessica Bearden
Northeast Structural Genomics Consortium

Publications:

PMID: 15808222

Title: Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium

Vector Map:

Vector Sequence:

Sequence:

TTCTCATGTTTGACAGCTTATCATCGATAAGCTAGCTTTAATGCGGTAGTTTATCACAGTTAAATTGCTAACGCAGTCAGGCACCGTGTATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGGATATCCGGATATAGTTCCTCCTTTCAGCAAAAAACCCCTCAAGACCCGTTTAGAGGCCCCAAGGGGTTATGCTAGTTATTGCTCAGCGGTGGCAGCAGCCAACTCAGCTTCCTTTCGGGCTTTGTTAGCAGCCGGATCTCGAGAAGCTTTGCGTCGACAGAGCTCGCAGGATCCGCAGAATTCGCCATATGGCTGTGATGGTGATGGTGATGGCCCATGGTATATCTCCTTCTTAAAGTTAAACAAAATTATTTCTAGAGGGGAATTGTTATCCGCTCACAATTCCCCTATAGTGAGTCGTATTAATTTCGCGGGATCGAGATCTCGATCCTCTACGCCGGACGCATCGTGGCCGGCATCACCGGCGCCACAGGTGCGGTTGCTGGCGCCTATATCGCCGACATCACCGATGGGGAAGATCGGGCTCGCCACTTCGGGCTCATGAGCGCTTGTTTCGGCGTGGGTATGGTGGCAGGCCCCGTGGCCGGGGGACTGTTGGGCGCCATCTCCTTGCATGCACCATTCCTTGCGGCGGCGGTGCTCAACGGCCTCAACCTACTACTGGGCTGCTTCCTAATGCAGGAGTCGCATAAGGGAGAGCGTCGAGATCCCGGACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAATGTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAACGCGGGAAAAAGTGGAAGCGGCGATGGCGGAGCTGAATTACATTCCCAACCGCGTGGCACAACAACTGGCGGGCAAACAGTCGTTGCTGATTGGCGTTGCCACCTCCAGTCTGGCCCTGCACGCGCCGTCGCAAATTGTCGCGGCGATTAAATCTCGCGCCGATCAACTGGGTGCCAGCGTGGTGGTGTCGATGGTAGAACGAAGCGGCGTCGAAGCCTGTAAAGCGGCGGTGCACAATCTTCTCGCGCAACGCGTCAGTGGGCTGATCATTAACTATCCGCTGGATGACCAGGATGCCATTGCTGTGGAAGCTGCCTGCACTAATGTTCCGGCGTTATTTCTTGATGTCTCTGACCAGACACCCATCAACAGTATTATTTTCTCCCATGAAGACGGTACGCGACTGGGCGTGGAGCATCTGGTCGCATTGGGTCACCAGCAAATCGCGCTGTTAGCGGGCCCATTAAGTTCTGTCTCGGCGCGTCTGCGTCTGGCTGGCTGGCATAAATATCTCACTCGCAATCAAATTCAGCCGATAGCGGAACGGGAAGGCGACTGGAGTGCCATGTCCGGTTTTCAACAAACCATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAACGATCAGATGGCGCTGGGCGCAATGCGCGCCATTACCGAGTCCGGGCTGCGCGTTGGTGCGGATATCTCGGTAGTGGGATACGACGATACCGAAGACAGCTCATGTTATATCCCGCCGTTAACCACCATCAAACAGGATTTTCGCCTGCTGGGGCAAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCAGCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTAAGTTAGCTCACTCATTAGGCACCGGGATCTCGACCGATGCCCTTGAGAGCCTTCAACCCAGTCAGCTCCTTCCGGTGGGCGCGGGGCATGACTATCGTCGCCGCACTTATGACTGTCTTCTTTATCATGCAACTCGTAGGACAGGTGCCGGCAGCGCTCTGGGTCATTTTCGGCGAGGACCGCTTTCGCTGGAGCGCGACGATGATCGGCCTGTCGCTTGCGGTATTCGGAATCTTGCACGCCCTCGCTCAAGCCTTCGTCACTGGTCCCGCCACCAAACGTTTCGGCGAGAAGCAGGCCATTATCGCCGGCATGGCGGCCGACGCGCTGGGCTACGTCTTGCTGGCGTTCGCGACGCGAGGCTGGATGGCCTTCCCCATTATGATTCTTCTCGCTTCCGGCGGCATCGGGATGCCCGCGTTGCAGGCCATGCTGTCCAGGCAGGTAGATGACGACCATCAGGGACAGCTTCAAGGATCGCTCGCGGCTCTTACCAGCCTAACTTCGATCACTGGACCGCTGATCGTCACGGCGATTTATGCCGCCTCGGCGAGCACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGAATGGAAGCCGGCGGCACCTCGCTAACGGATTCACCACTCCAAGAATTGGAGCCAATCAATTCTTGCGGAGAACTGTGAATGCGCAAACCAACCCTTGGCAGAACATATCCATCGCGTCCGCCATCTCCAGCAGCCGCACGCGGCGCATCTCGGGCAGCGTTGGGTCCTGGCCACGGGTGCGCATGATCGTGCTCCTGTCGTTGAGGACCCGGCTAGGCTGGCGGGGTTGCCTTACTGGTTAGCAGAATGAATCACCGATACGCGAGCGAACGTGAAGCGACTGCTGCTGCAAAACGTCTGCGACCTGAGCAACAACATGAATGGTCTTCGGTTTCCGTGTTTCGTAAAGTCTGGAAACGCGGAAGTCAGCGCCCTGCACCATTATGTTCCGGATCTGCATCGCAGGATGCTGCTGGCTACCCTGTGGAACACCTACATCTGTATTAACGAAGCGCTGGCATTGACCCTGAGTGATTTTTCTCTGGTCCCGCCGCATCCATACCGCCAGTTGTTTACCCTCACAACGTTCCAGTAACCGGGCATGTTCATCATCAGTAACCCGTATCGTGAGCATCCTCTCTCGTTTCATCGGTATCATTACCCCCATGAACAGAAATCCCCCTTACACGGAGGCATCAGTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACAGCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGCACCATATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTGCAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAACACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGCCCTTTCGTCTTCAAGAA

Vector Features:

Type

Name

Description

Start

End

MCS

MCS (includes SphI, XbaI, EcoRI, BamHI, XhoI)

327

452

bacterial origin

ori

ColE1 origin of replication

3881

4563

gene fragment

ROP

repressor of primer ROP sequence

3281

3457

primer

T7 forward

T7 forward sequencing primer

481

496

primer

T7 reverse

T7 reverse sequencing primer

257

275

promoter

T7 promoter

478

497

repressor binding site

lacO

lacO regulatory sequence

456

478

selectable marker

AmpR

ampicillin resistance gene

4661

5230

tag

His

N-terminal 6x His tag

387

404

trxn termination sequence

T7 term

T7 termination sequence

257

275

Gene

Gene Symbol :

MAPK8IP1

Symbol Nomenclature :

MAPK8IP1

SYNONYM :

IB1; JIP-1; JIP1; PRKM8IP

Designation :

Full Nomenclature :

mitogen-activated protein kinase 8 interacting protein 1

GENEID :

9479

GI :

4885433

GenBank Accession :

NM_005456

HGNC :

6882

MIM :

604641

Vega :

OTTHUMG00000134324

Target GenBank:

None

Reference Sequence Alignment

  

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      ------------------------------ATGCAGCTGGTGCTGAAGATGGATTCGAGC
NM_005456.3         ATGGCGGAGCGAGAAAGCGGCGGCCTGGGAGGGGGGGCCGCGTCCCCGCCCGCCGCCTCC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CCAGACAATGACAGCTGGTTGGAGGATCAATGGGAGCGCTGGCTCACCCATGACATCAGC
NM_005456.3         CCGTTCCTGGGGCTGCACATCGCTTCGCCTCCCAATTTCAGGCTCACCCATGACATCAGC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CTGGAGGAGTTTGAGGATGAAGACCTCTCGGAGATCACTGATGAGTGTGGCATCAGCTTA
NM_005456.3         CTGGAGGAGTTTGAGGATGAAGACCTCTCGGAGATCACTGATGAGTGTGGCATCAGCTTA
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CAGTGCAAAGACACCCTGTCCTTACGGCCCCCGCGCGCCGGGCTGCTCTCTGCGGGCGGC
NM_005456.3         CAGTGCAAAGACACCCTGTCCTTACGGCCCCCGCGCGCCGGGCTGCTCTCTGCGGGCGGC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      GGCGGCGCGGGGAGCCGGTTGCAGGCCGAGATGCTGCAGATGGACCTGATCGACGCGACG
NM_005456.3         GGCGGCGCGGGGAGCCGGTTGCAGGCCGAGATGCTGCAGATGGACCTGATCGACGCGACG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      GGGGACACTCCCGGGGCCGAGGACGACGAGGAGGACGACGACGAGGAGCGCGCGGCCCGG
NM_005456.3         GGGGACACTCCCGGGGCCGAGGACGACGAGGAGGACGACGACGAGGAGCGCGCGGCCCGG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CGGCCGGGAGCGGGGCCGCCCAAGGCCGAGTCCGGCCAGGAGCCGGCGTCCCGCGGCCAG
NM_005456.3         CGGCCGGGAGCGGGGCCGCCCAAGGCCGAGTCCGGCCAGGAGCCGGCGTCCCGCGGCCAG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      GGCCAGAGCCAAGGCCAGAGCCAGGGCCCGGGCAGCGGGGACACGTACCGGCCCAAGCGG
NM_005456.3         GGCCAGAGCCAAGGCCAGAGCCAGGGCCCGGGCAGCGGGGACACGTACCGGCCCAAGCGG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CCCACCACGCTCAACCTCTTTCCGCAGGTGCCGCGGTCTCAGGACACACTGAATAATAAT
NM_005456.3         CCCACCACGCTCAACCTCTTTCCGCAGGTGCCGCGGTCTCAGGACACACTGAATAATAAT
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      TCTCTGGGCAAAAAGCACAGTTGGCAGGATCGGGTGTCTCGATCATCCTCACCCCTGAAG
NM_005456.3         TCTCTGGGCAAAAAGCACAGTTGGCAGGATCGGGTGTCTCGATCATCCTCACCCCTGAAG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      ACAGGGGAGCAGACACCACCGCATGAACACATCTGCCTGAGCGATGAGCTGCCCCCCCAG
NM_005456.3         ACAGGGGAGCAGACACCACCGCATGAACACATCTGCCTGAGCGATGAGCTGCCCCCCCAG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      AGCGGCCCCGCCCCCACCACAGATCGAGGCACCTCCACCGACAGCCCTTGCCGCCGCAGC
NM_005456.3         AGCGGCCCCGCCCCCACCACAGATCGAGGCACCTCCACCGACAGCCCTTGCCGCCGCAGC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      ACAGCCACCCAGATGGCACCTCCGGGTGGTCCCCCTGCTGCCCCGCCTGGGGGTCGGGGC
NM_005456.3         ACAGCCACCCAGATGGCACCTCCGGGTGGTCCCCCTGCTGCCCCGCCTGGGGGTCGGGGC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CACTCGCATCGAGACCGAATCCACTACCAGGCCGATGTGCGACTAGAGGCCACTGAGGAG
NM_005456.3         CACTCGCATCGAGACCGAATCCACTACCAGGCCGATGTGCGACTAGAGGCCACTGAGGAG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      ATCTACCTGACCCCAGTGCAGAGGCCCCCAGACGCTGCAGAGCCCACCTCCGCCTTCCTG
NM_005456.3         ATCTACCTGACCCCAGTGCAGAGGCCCCCAGACGCTGCAGAGCCCACCTCCGCCTTCCTG
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      CCGCCCACTGAGAGCCGGATGTCAGTCAGCTCCGATCCAGACCCTGCCGCCTACCCCTCC
NM_005456.3         CCGCCCACTGAGAGCCGGATGTCAGTCAGCTCCGATCCAGACCCTGCCGCCTACCCCTCC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      ACGGCAGGGCGGCCGCACCCCTCCATCAGTGAAGAGGAAGAGGGCTTCGACTGCCTGTCG
NM_005456.3         ACGGCAGGGCGGCCGCACCCCTCCATCAGTGAAGAGGAAGAGGGCTTCGACTGCCTGTCG
                                                                                

HsCD00585251        ---------------------------------AAGGAGATATACCATGGGCCATCACCA
XM_005253226.1      TCCCCAGAGCGGGCTGAGCCCCCAGGCGGAGGGTGGCGGGGGAGCCTGGGGGAGCCGCCG
NM_005456.3         TCCCCAGAGCGGGCTGAGCCCCCAGGCGGAGGGTGGCGGGGGAGCCTGGGGGAGCCGCCG
                                                     :.* .*. .:.**: *** .. *.**.

HsCD00585251        TCACCATCACAGCCATATGG----------------------------------------
XM_005253226.1      CCACCTCCACGGGCCTCTCTGAGCTCGGACACCAGCGCCCTGTCCTATGACTCTGTCAAG
NM_005456.3         CCACCTCCACGGGCCTCTCTGAGCTCGGACACCAGCGCCCTGTCCTATGACTCTGTCAAG
                     ****: ***.* *.*.*                                          

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      TACACGCTGGTGGTAGATGAGCATGCACAGCTGGAGCTGGTGAGCCTGCGGCCGTGCTTC
NM_005456.3         TACACGCTGGTGGTAGATGAGCATGCACAGCTGGAGCTGGTGAGCCTGCGGCCGTGCTTC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      GGAGACTACAGTGACGAGAGTGACTCTGCCACCGTCTATGACAACTGTGCCTCCGTCTCC
NM_005456.3         GGAGACTACAGTGACGAGAGTGACTCTGCCACCGTCTATGACAACTGTGCCTCCGTCTCC
                                                                                

HsCD00585251        ------------------------------------------------------------
XM_005253226.1      TCGCCCTATGAGTCGGCCATCGGAGAGGAATATGAGGAGGCCCCGCGGCCCCAGCCCCCT
NM_005456.3         TCGCCCTATGAGTCGGCCATCGGAGAGGAATATGAGGAGGCCCCGCGGCCCCAGCCCCCT
                                                                                

HsCD00585251        ----------------------------------------TCCATTTCTCCAAGAAATTC
XM_005253226.1      GCCTGCCTCTCCGAGGACTCCACGCCTGATGAACCCGACGTCCATTTCTCCAAGAAATTC
NM_005456.3         GCCTGCCTCTCCGAGGACTCCACGCCTGATGAACCCGACGTCCATTTCTCCAAGAAATTC
                                                            ********************

HsCD00585251        CTGAACGTCTTCATGAGTGGCCGCTCCCGCTCCTCCAGTGCTGAGTCCTTCGGGCTGTTC
XM_005253226.1      CTGAACGTCTTCATGAGTGGCCGCTCCCGCTCCTCCAGTGCTGAGTCCTTCGGGCTGTTC
NM_005456.3         CTGAACGTCTTCATGAGTGGCCGCTCCCGCTCCTCCAGTGCTGAGTCCTTCGGGCTGTTC
                    ************************************************************

HsCD00585251        TCCTGCATCATCAACGGGGAGGAGCAGGAGCAGACCCACCGGGCCATATTCAGGTTTGTG
XM_005253226.1      TCCTGCATCATCAACGGGGAGGAGCAGGAGCAGACCCACCGGGCCATATTCAGGTTTGTG
NM_005456.3         TCCTGCATCATCAACGGGGAGGAGCAGGAGCAGACCCACCGGGCCATATTCAGGTTTGTG
                    ************************************************************

HsCD00585251        CCTCGACACGAAGACGAACTTGAGCTGGAAGTGGATGACCCTCTGCTAGTGGAGCTCCAG
XM_005253226.1      CCTCGACACGAAGACGAACTTGAGCTGGAAGTGGATGACCCTCTGCTAGTGGAGCTCCAG
NM_005456.3         CCTCGACACGAAGACGAACTTGAGCTGGAAGTGGATGACCCTCTGCTAGTGGAGCTCCAG
                    ************************************************************

HsCD00585251        GCTGAAGACTACTGGTACGAGGCCTACAACATGCGCACTGGTGCCCGGGGTGTCTTTCCT
XM_005253226.1      GCTGAAGACTACTGGTACGAGGCCTACAACATGCGCACTGGTGCCCGGGGTGTCTTTCCT
NM_005456.3         GCTGAAGACTACTGGTACGAGGCCTACAACATGCGCACTGGTGCCCGGGGTGTCTTTCCT
                    ************************************************************

HsCD00585251        GCCTATTACGCCATCGAGGTCACCAAGGAGCCCGAGCACATGGCAGCCCTGGCCAAAAAC
XM_005253226.1      GCCTATTACGCCATCGAGGTCACCAAGGAGCCCGAGCACATGGCAGCCCTGGCCAAAAAC
NM_005456.3         GCCTATTACGCCATCGAGGTCACCAAGGAGCCCGAGCACATGGCAGCCCTGGCCAAAAAC
                    ************************************************************

HsCD00585251        AGTGACTGGGTGGACCAGTTCCGGGTGAAGTTCCTGGGCTCAGTCCAGGTTCCCTATCAC
XM_005253226.1      AGTGACTGGGTGGACCAGTTCCGGGTGAAGTTCCTGGGCTCAGTCCAGGTTCCCTATCAC
NM_005456.3         AGTGACTGGGTGGACCAGTTCCGGGTGAAGTTCCTGGGCTCAGTCCAGGTTCCCTATCAC
                    ************************************************************

HsCD00585251        AAGGGCAATGACGTCCTCTGTGCTGCTATGCAAAAGATTGCCACCACCCGCCGGCTCACC
XM_005253226.1      AAGGGCAATGACGTCCTCTGTGCTGCTATGCAAAAGATTGCCACCACCCGCCGGCTCACC
NM_005456.3         AAGGGCAATGACGTCCTCTGTGCTGCTATGCAAAAGATTGCCACCACCCGCCGGCTCACC
                    ************************************************************

HsCD00585251        GTGCACTTTAACCCGCCCTCCAGCTGTGTCCTGGAGATCAGCGTGCGGGGTGTGAAGATA
XM_005253226.1      GTGCACTTTAACCCGCCCTCCAGCTGTGTCCTGGAGATCAGCGTGCGGGGTGTGAAGATA
NM_005456.3         GTGCACTTTAACCCGCCCTCCAGCTGTGTCCTGGAGATCAGCGTGCGGGGTGTGAAGATA
                    ************************************************************

HsCD00585251        GGCGTCAAGGCCGATGACTCCCAGGAGGCCAAGGGGAATAAATGTAGCCACTTTTTCCAG
XM_005253226.1      GGCGTCAAGGCCGATGACTCCCAGGAGGCCAAGGGGAATAAATGTAGCCACTTTTTCCAG
NM_005456.3         GGCGTCAAGGCCGATGACTCCCAGGAGGCCAAGGGGAATAAATGTAGCCACTTTTTCCAG
                    ************************************************************

HsCD00585251        TTAAAAAACATCTCTTTCTGCGGATATCATCCAAAGAACAACAAGTACTTTGGGTTCATC
XM_005253226.1      TTAAAAAACATCTCTTTCTGCGGATATCATCCAAAGAACAACAAGTACTTTGGGTTCATC
NM_005456.3         TTAAAAAACATCTCTTTCTGCGGATATCATCCAAAGAACAACAAGTACTTTGGGTTCATC
                    ************************************************************

HsCD00585251        ACCAAGCACCCCGCCGACCACCGGTTTGCCTGCCACGTCTTTGTGTCTGAAGACTCCACC
XM_005253226.1      ACCAAGCACCCCGCCGACCACCGGTTTGCCTGCCACGTCTTTGTGTCTGAAGACTCCACC
NM_005456.3         ACCAAGCACCCCGCCGACCACCGGTTTGCCTGCCACGTCTTTGTGTCTGAAGACTCCACC
                    ************************************************************

HsCD00585251        AAAGCCCTGGCAGAGTCCGTGGGGAGAGCATTCCAGCAGTTCTACAAGCAGTTTGTGGAG
XM_005253226.1      AAAGCCCTGGCAGAGTCCGTGGGGAGAGCATTCCAGCAGTTCTACAAGCAGTTTGTGGAG
NM_005456.3         AAAGCCCTGGCAGAGTCCGTGGGGAGAGCATTCCAGCAGTTCTACAAGCAGTTTGTGGAG
                    ************************************************************

HsCD00585251        TACACCTGCCCCACATAG------------------
XM_005253226.1      TACACCTGCCCCACAGAAGATATCTACCTGGAGTAG
NM_005456.3         TACACCTGCCCCACAGAAGATATCTACCTGGAGTAG
                    *************** *.